Restriction map for
pFN21AB6286
(Linear) (Six-base) MAPSORT of: KIBB6286.seq Check: 4037 from: 1 to: 2352
KIBB6286.orf
With 182 enzymes: SgfI *
July 3, 2012 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1593 1787 2154 2352
Size: 1593 194 367 198
Fragments arranged by size:
1593 367 198 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 819 1596 2352
Size: 129 690 777 756
Fragments arranged by size:
777 756 690 129
AfeI AGC'GCT
Cuts at: 0 1579 2352
Size: 1579 773
AflIII A'CryG_T
Cuts at: 0 1640 2352
Size: 1640 712
AleI CACnn'nnGTG
Cuts at: 0 1457 2352
Size: 1457 895
AlwNI CAG_nnn'CTG
Cuts at: 0 510 886 1023 1186 1212 1257 2352
Size: 510 376 137 163 26 45 1095
Fragments arranged by size:
1095 510 376 163 137 45 26
ApaI G_GGCC'C
Cuts at: 0 255 2107 2352
Size: 255 1852 245
Fragments arranged by size:
1852 255 245
ApaLI G'TGCA_C
Cuts at: 0 169 782 1450 1606 2035 2352
Size: 169 613 668 156 429 317
Fragments arranged by size:
668 613 429 317 169 156
AvaI C'yCGr_G
Cuts at: 0 486 2146 2216 2352
Size: 486 1660 70 136
Fragments arranged by size:
1660 486 136 70
BanI G'GyrC_C
Cuts at: 0 138 312 710 794 985 1168 1290 1775
Size: 138 174 398 84 191 183 122 485
Cuts at: 1775 1984 2352
Size: 209 368
Fragments arranged by size:
485 398 368 209 191 183 174 138
122 84
BanII G_rGCy'C
Cuts at: 0 255 341 518 700 1058 1076 1681 2107
Size: 255 86 177 182 358 18 605 426
Cuts at: 2107 2352
Size: 245
Fragments arranged by size:
605 426 358 255 245 182 177 86
18
BbeI G_GCGC'C
Cuts at: 0 142 2352
Size: 142 2210
BbsI GAAGACnn'nnnn_
Cuts at: 0 1874 2352
Size: 1874 478
BbvCI CC'TCA_GC
Cuts at: 0 449 1284 2352
Size: 449 835 1068
Fragments arranged by size:
1068 835 449
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1921 1955 2352
Size: 34 1887 34 397
Fragments arranged by size:
1887 397 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 379 1279 2218 2352
Size: 379 900 939 134
Fragments arranged by size:
939 900 379 134
Bme1580I G_kGCm'C
Cuts at: 0 173 255 425 786 988 1173 1295 1454
Size: 173 82 170 361 202 185 122 159
Cuts at: 1454 1610 1987 2039 2107 2203 2352
Size: 156 377 52 68 96 149
Fragments arranged by size:
377 361 202 185 173 170 159 156
149 122 96 82 68 52
BmgBI CAC'GTC
Cuts at: 0 852 1008 2352
Size: 852 156 1344
Fragments arranged by size:
1344 852 156
BmtI G_CTAG'C
Cuts at: 0 2048 2352
Size: 2048 304
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 390 1695 2352
Size: 390 1305 657
Fragments arranged by size:
1305 657 390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 908 914 1068 1195 1279 1369 1645 1825
Size: 908 6 154 127 84 90 276 180
Cuts at: 1825 2305 2350 2352
Size: 480 45 2
Fragments arranged by size:
908 480 276 180 154 127 90 84
45 6 2
Bpu10I CC'TnA_GC
Cuts at: 0 449 1284 2352
Size: 449 835 1068
Fragments arranged by size:
1068 835 449
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 784 1165 1417 2352
Size: 604 180 381 252 935
Fragments arranged by size:
935 604 381 252 180
BsaI GGTCTCn'nnnn_
Cuts at: 0 1318 1948 2352
Size: 1318 630 404
Fragments arranged by size:
1318 630 404
BsaAI yAC'GTr
Cuts at: 0 877 1455 1641 1695 2352
Size: 877 578 186 54 657
Fragments arranged by size:
877 657 578 186 54
BsaHI Gr'CG_yC
Cuts at: 0 139 2352
Size: 139 2213
BsaWI w'CCGG_w
Cuts at: 0 258 2352
Size: 258 2094
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 733 763 1882 1912 2352
Size: 733 30 1119 30 440
Fragments arranged by size:
1119 733 440 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 349 1177 1642 1828 2104 2352
Size: 349 828 465 186 276 248
Fragments arranged by size:
828 465 349 276 248 186
BseYI C'CCAG_C
Cuts at: 0 115 297 459 510 1156 1205 2100 2352
Size: 115 182 162 51 646 49 895 252
Fragments arranged by size:
895 646 252 182 162 115 51 49
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 801 899 1096 1270 1338 2044 2089
Size: 104 697 98 197 174 68 706 45
Cuts at: 2089 2352
Size: 263
Fragments arranged by size:
706 697 263 197 174 104 98 68
45
BsiEI CG_ry'CG
Cuts at: 0 32 183 1743 2352
Size: 32 151 1560 609
Fragments arranged by size:
1560 609 151 32
BsiHKAI G_wGCw'C
Cuts at: 0 173 751 786 1076 1454 1610 1760 2039
Size: 173 578 35 290 378 156 150 279
Cuts at: 2039 2352
Size: 313
Fragments arranged by size:
578 378 313 290 279 173 156 150
35
BsmBI CGTCTCn'nnnn_
Cuts at: 0 458 1068 2086 2352
Size: 458 610 1018 266
Fragments arranged by size:
1018 610 458 266
Bsp1286I G_dGCh'C
Cuts at: 0 173 255 341 425 518 700 751 786
Size: 173 82 86 84 93 182 51 35
Cuts at: 786 988 1058 1076 1173 1295 1454 1610 1681
Size: 202 70 18 97 122 159 156 71
Cuts at: 1681 1760 1987 2039 2107 2203 2352
Size: 79 227 52 68 96 149
Fragments arranged by size:
227 202 182 173 159 156 149 122
97 96 93 86 84 82 79 71
70 68 52 51 35 18
BspEI T'CCGG_A
Cuts at: 0 258 2352
Size: 258 2094
BspHI T'CATG_A
Cuts at: 0 2012 2352
Size: 2012 340
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 871 1115 1436 1593 1787 2154 2352
Size: 871 244 321 157 194 367 198
Fragments arranged by size:
871 367 321 244 198 194 157
BsrBI CCG'CTC
Cuts at: 0 34 1548 2352
Size: 34 1514 804
Fragments arranged by size:
1514 804 34
BsrDI GCAATG_nn'
Cuts at: 0 1505 2135 2352
Size: 1505 630 217
Fragments arranged by size:
1505 630 217
BsrFI r'CCGG_y
Cuts at: 0 315 716 901 1236 1526 1772 2050 2352
Size: 315 401 185 335 290 246 278 302
Fragments arranged by size:
401 335 315 302 290 278 246 185
BssHII G'CGCG_C
Cuts at: 0 1149 1394 2352
Size: 1149 245 958
Fragments arranged by size:
1149 958 245
BssSI C'ACGA_G
Cuts at: 0 658 2352
Size: 658 1694
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2034 2352
Size: 2034 318
BstXI CCAn_nnnn'nTGG
Cuts at: 0 348 1519 2178 2352
Size: 348 1171 659 174
Fragments arranged by size:
1171 659 348 174
BstYI r'GATC_y
Cuts at: 0 2279 2352
Size: 2279 73
BtgI C'CryG_G
Cuts at: 0 1659 1743 1841 1901 1967 2081 2255 2352
Size: 1659 84 98 60 66 114 174 97
Fragments arranged by size:
1659 174 114 98 97 84 66 60
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 25 2292 2352
Size: 25 2267 60
Fragments arranged by size:
2267 60 25
BtsI GCAGTG_nn'
Cuts at: 0 23 1223 1326 1405 1937 2352
Size: 23 1200 103 79 532 415
Fragments arranged by size:
1200 532 415 103 79 23
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 654 689 2352
Size: 654 35 1663
Fragments arranged by size:
1663 654 35
DraIII CAC_nnn'GTG
Cuts at: 0 360 969 1881 2352
Size: 360 609 912 471
Fragments arranged by size:
912 609 471 360
DrdI GACnn_nn'nnGTC
Cuts at: 0 856 2259 2352
Size: 856 1403 93
Fragments arranged by size:
1403 856 93
EaeI y'GGCC_r
Cuts at: 0 29 180 476 567 1271 1521 1740 2052
Size: 29 151 296 91 704 250 219 312
Cuts at: 2052 2168 2186 2352
Size: 116 18 166
Fragments arranged by size:
704 312 296 250 219 166 151 116
91 29 18
EagI C'GGCC_G
Cuts at: 0 29 180 1740 2352
Size: 29 151 1560 612
Fragments arranged by size:
1560 612 151 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 469 2352
Size: 469 1883
EcoICRI GAG'CTC
Cuts at: 0 1074 2352
Size: 1074 1278
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 819 908 914 1068 1195 1279 1369
Size: 129 690 89 6 154 127 84 90
Cuts at: 1369 1596 1645 1825 2305 2350 2352
Size: 227 49 180 480 45 2
Fragments arranged by size:
690 480 227 180 154 129 127 90
89 84 49 45 6 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1804 2352
Size: 1804 548
EcoO109I rG'GnC_Cy
Cuts at: 0 252 264 483 677 1298 2352
Size: 252 12 219 194 621 1054
Fragments arranged by size:
1054 621 252 219 194 12
FspI TGC'GCA
Cuts at: 0 1783 2352
Size: 1783 569
HaeII r_GCGC'y
Cuts at: 0 142 738 1467 1581 2051 2101 2352
Size: 142 596 729 114 470 50 251
Fragments arranged by size:
729 596 470 251 142 114 50
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 18 50 1882 1914 2352
Size: 18 32 1832 32 438
Fragments arranged by size:
1832 438 32 32 18
KasI G'GCGC_C
Cuts at: 0 138 2352
Size: 138 2214
MscI TGG'CCA
Cuts at: 0 1273 1523 2170 2352
Size: 1273 250 647 182
Fragments arranged by size:
1273 647 250 182
MslI CAynn'nnrTG
Cuts at: 0 346 361 728 1457 1517 2017 2352
Size: 346 15 367 729 60 500 335
Fragments arranged by size:
729 500 367 346 335 60 15
MspA1I CmG'CkG
Cuts at: 0 119 185 249 1233 1257 1288 1475 1843
Size: 119 66 64 984 24 31 187 368
Cuts at: 1843 2328 2352
Size: 485 24
Fragments arranged by size:
984 485 368 187 119 66 64 31
24 24
NaeI GCC'GGC
Cuts at: 0 317 903 2052 2352
Size: 317 586 1149 300
Fragments arranged by size:
1149 586 317 300
NarI GG'CG_CC
Cuts at: 0 139 2352
Size: 139 2213
NcoI C'CATG_G
Cuts at: 0 2081 2255 2352
Size: 2081 174 97
Fragments arranged by size:
2081 174 97
NgoMIV G'CCGG_C
Cuts at: 0 315 901 2050 2352
Size: 315 586 1149 302
Fragments arranged by size:
1149 586 315 302
NheI G'CTAG_C
Cuts at: 0 2044 2352
Size: 2044 308
NotI GC'GGCC_GC
Cuts at: 0 180 2352
Size: 180 2172
NspI r_CATG'y
Cuts at: 0 2293 2352
Size: 2293 59
PasI CC'CwG_GG
Cuts at: 0 1029 2352
Size: 1029 1323
PflMI CCAn_nnn'nTGG
Cuts at: 0 309 360 1212 2352
Size: 309 51 852 1140
Fragments arranged by size:
1140 852 309 51
PfoI T'CCnGG_A
Cuts at: 0 770 2282 2352
Size: 770 1512 70
Fragments arranged by size:
1512 770 70
PmlI CAC'GTG
Cuts at: 0 877 1455 1641 1695 2352
Size: 877 578 186 54 657
Fragments arranged by size:
877 657 578 186 54
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1705 1737 2352
Size: 1705 32 615
Fragments arranged by size:
1705 615 32
PpuMI rG'GwC_Cy
Cuts at: 0 1298 2352
Size: 1298 1054
PspOMI G'GGCC_C
Cuts at: 0 251 2103 2352
Size: 251 1852 249
Fragments arranged by size:
1852 251 249
PstI C_TGCA'G
Cuts at: 0 1657 2352
Size: 1657 695
PvuII CAG'CTG
Cuts at: 0 1233 2328 2352
Size: 1233 1095 24
Fragments arranged by size:
1233 1095 24
SacI G_AGCT'C
Cuts at: 0 1076 2352
Size: 1076 1276
SacII CC_GC'GG
Cuts at: 0 1844 2352
Size: 1844 508
SexAI A'CCwGG_T
Cuts at: 0 965 2352
Size: 965 1387
SfcI C'TryA_G
Cuts at: 0 941 1653 2352
Size: 941 712 699
Fragments arranged by size:
941 712 699
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1279 2352
Size: 1279 1073
SfoI GGC'GCC
Cuts at: 0 140 2352
Size: 140 2212
SgrAI Cr'CCGG_yG
Cuts at: 0 716 1526 2352
Size: 716 810 826
Fragments arranged by size:
826 810 716
SmaI CCC'GGG
Cuts at: 0 488 2218 2352
Size: 488 1730 134
Fragments arranged by size:
1730 488 134
SmlI C'TyrA_G
Cuts at: 0 619 799 1144 1432 2352
Size: 619 180 345 288 920
Fragments arranged by size:
920 619 345 288 180
SphI G_CATG'C
Cuts at: 0 2293 2352
Size: 2293 59
SrfI GCCC'GGGC
Cuts at: 0 2218 2352
Size: 2218 134
StuI AGG'CCT
Cuts at: 0 241 1282 1411 1423 2352
Size: 241 1041 129 12 929
Fragments arranged by size:
1041 929 241 129 12
StyI C'CwwG_G
Cuts at: 0 107 206 692 980 1418 2081 2255 2352
Size: 107 99 486 288 438 663 174 97
Fragments arranged by size:
663 486 438 288 174 107 99 97
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1319 1930 2002 2352
Size: 1319 611 72 350
Fragments arranged by size:
1319 611 350 72
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 646 678 2352
Size: 646 32 1674
Fragments arranged by size:
1674 646 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 294 2253 2352
Size: 294 1959 99
Fragments arranged by size:
1959 294 99
XmaI C'CCGG_G
Cuts at: 0 486 2216 2352
Size: 486 1730 136
Fragments arranged by size:
1730 486 136
XmnI GAAnn'nnTTC
Cuts at: 0 1720 2352
Size: 1720 632
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
Bme1580I BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI DraIII DrdI EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NotI
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PstI PvuII SacI SacII SexAI SfcI SfiI SfoI
SgrAI SmaI SmlI SphI SrfI StuI StyI TaqII
TstI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BlpI BmrI BsaBI BsiWI
BsmI BsrGI BstBI BstEII BstZ17I Bsu36I ClaI DraI
EarI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI KpnI MfeI MluI MmeI NdeI NruI NsiI
PacI PciI PmeI PshAI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SgfI SnaBI
SpeI SspI SwaI TatI Tth111I XbaI XhoI ZraI