Restriction map for
pFN21AB6280
(Linear) (Six-base) MAPSORT of: KIBB6280.seq Check: 8840 from: 1 to: 2343
KIBB6280.orf
With 182 enzymes: SgfI *
July 3, 2012 16:19 ..
AccI GT'mk_AC
Cuts at: 0 614 1237 1603 1661 2343
Size: 614 623 366 58 682
Fragments arranged by size:
682 623 614 366 58
Acc65I G'GTAC_C
Cuts at: 0 1062 2343
Size: 1062 1281
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 908 1559 1975 2096 2343
Size: 466 442 651 416 121 247
Fragments arranged by size:
651 466 442 416 247 121
AgeI A'CCGG_T
Cuts at: 0 1935 2343
Size: 1935 408
AhdI GACnn_n'nnGTC
Cuts at: 0 2025 2343
Size: 2025 318
AleI CACnn'nnGTG
Cuts at: 0 1354 2343
Size: 1354 989
AlwNI CAG_nnn'CTG
Cuts at: 0 806 2343
Size: 806 1537
ApoI r'AATT_y
Cuts at: 0 637 1098 1258 2169 2181 2343
Size: 637 461 160 911 12 162
Fragments arranged by size:
911 637 461 162 160 12
AvaI C'yCGr_G
Cuts at: 0 487 505 1797 2343
Size: 487 18 1292 546
Fragments arranged by size:
1292 546 487 18
BanI G'GyrC_C
Cuts at: 0 278 761 1062 2343
Size: 278 483 301 1281
Fragments arranged by size:
1281 483 301 278
BanII G_rGCy'C
Cuts at: 0 133 494 591 722 1639 2343
Size: 133 361 97 131 917 704
Fragments arranged by size:
917 704 361 133 131 97
BbsI GAAGACnn'nnnn_
Cuts at: 0 1077 1775 2276 2343
Size: 1077 698 501 67
Fragments arranged by size:
1077 698 501 67
BbvCI CC'TCA_GC
Cuts at: 0 964 2343
Size: 964 1379
BglI GCCn_nnn'nGGC
Cuts at: 0 235 2343
Size: 235 2108
BlpI GC'TnA_GC
Cuts at: 0 127 2343
Size: 127 2216
BmgBI CAC'GTC
Cuts at: 0 336 1251 1301 2343
Size: 336 915 50 1042
Fragments arranged by size:
1042 915 336 50
BmrI ACTGGGnnnn_n'
Cuts at: 0 846 1112 1240 1307 1643 2343
Size: 846 266 128 67 336 700
Fragments arranged by size:
846 700 336 266 128 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 178 921 1058 1255 2290 2343
Size: 125 53 743 137 197 1035 53
Fragments arranged by size:
1035 743 197 137 125 53 53
Bpu10I CC'TnA_GC
Cuts at: 0 964 2343
Size: 964 1379
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 697 1551 1653 2343
Size: 697 854 102 690
Fragments arranged by size:
854 697 690 102
BsaI GGTCTCn'nnnn_
Cuts at: 0 841 2343
Size: 841 1502
BsaAI yAC'GTr
Cuts at: 0 427 2343
Size: 427 1916
BsaHI Gr'CG_yC
Cuts at: 0 170 2343
Size: 170 2173
BsaWI w'CCGG_w
Cuts at: 0 1465 1935 2343
Size: 1465 470 408
Fragments arranged by size:
1465 470 408
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 919 949 1388 1418 2343
Size: 664 30 225 30 439 30 925
Fragments arranged by size:
925 664 439 225 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 685 914 1815 2343
Size: 685 229 901 528
Fragments arranged by size:
901 685 528 229
BseYI C'CCAG_C
Cuts at: 0 603 880 1345 2100 2343
Size: 603 277 465 755 243
Fragments arranged by size:
755 603 465 277 243
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1673 1916 2343
Size: 1673 243 427
Fragments arranged by size:
1673 427 243
BsiEI CG_ry'CG
Cuts at: 0 179 230 597 601 2343
Size: 179 51 367 4 1742
Fragments arranged by size:
1742 367 179 51 4
BsiHKAI G_wGCw'C
Cuts at: 0 494 722 1639 2343
Size: 494 228 917 704
Fragments arranged by size:
917 704 494 228
BsmI GAATG_Cn'
Cuts at: 0 1370 1764 2343
Size: 1370 394 579
Fragments arranged by size:
1370 579 394
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 2343
Size: 667 1676
Bsp1286I G_dGCh'C
Cuts at: 0 133 494 591 722 1639 2343
Size: 133 361 97 131 917 704
Fragments arranged by size:
917 704 361 133 131 97
BspEI T'CCGG_A
Cuts at: 0 1465 2343
Size: 1465 878
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 1074 2343
Size: 428 646 1269
Fragments arranged by size:
1269 646 428
BsrBI CCG'CTC
Cuts at: 0 197 235 2343
Size: 197 38 2108
Fragments arranged by size:
2108 197 38
BsrDI GCAATG_nn'
Cuts at: 0 1212 1907 2343
Size: 1212 695 436
Fragments arranged by size:
1212 695 436
BsrFI r'CCGG_y
Cuts at: 0 596 623 778 821 1935 2343
Size: 596 27 155 43 1114 408
Fragments arranged by size:
1114 596 408 155 43 27
BssHII G'CGCG_C
Cuts at: 0 565 2343
Size: 565 1778
BssSI C'ACGA_G
Cuts at: 0 460 2343
Size: 460 1883
BstEII G'GTnAC_C
Cuts at: 0 2089 2343
Size: 2089 254
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1656 2343
Size: 981 675 687
Fragments arranged by size:
981 687 675
BstYI r'GATC_y
Cuts at: 0 1454 1693 2343
Size: 1454 239 650
Fragments arranged by size:
1454 650 239
BstZ17I GTA'TAC
Cuts at: 0 1662 2343
Size: 1662 681
BtgI C'CryG_G
Cuts at: 0 591 2343
Size: 591 1752
BtsI GCAGTG_nn'
Cuts at: 0 218 355 1169 1510 1661 2343
Size: 218 137 814 341 151 682
Fragments arranged by size:
814 682 341 218 151 137
EaeI y'GGCC_r
Cuts at: 0 227 527 594 598 767 2343
Size: 227 300 67 4 169 1576
Fragments arranged by size:
1576 300 227 169 67 4
EagI C'GGCC_G
Cuts at: 0 227 594 598 2343
Size: 227 367 4 1745
Fragments arranged by size:
1745 367 227 4
EarI CTCTTCn'nnn_
Cuts at: 0 12 1038 1994 2343
Size: 12 1026 956 349
Fragments arranged by size:
1026 956 349 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 256 2343
Size: 55 201 2087
Fragments arranged by size:
2087 201 55
EcoICRI GAG'CTC
Cuts at: 0 492 720 1637 2343
Size: 492 228 917 706
Fragments arranged by size:
917 706 492 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 178 466 908 921 1058 1255 1559
Size: 125 53 288 442 13 137 197 304
Cuts at: 1559 1975 2096 2290 2343
Size: 416 121 194 53
Fragments arranged by size:
442 416 304 288 197 194 137 125
121 53 53 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 445 793 1676 2343
Size: 445 348 883 667
Fragments arranged by size:
883 667 445 348
EcoO109I rG'GnC_Cy
Cuts at: 0 1114 2343
Size: 1114 1229
EcoRI G'AATT_C
Cuts at: 0 1258 2343
Size: 1258 1085
EcoRV GAT'ATC
Cuts at: 0 1022 2343
Size: 1022 1321
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1647 2256 2343
Size: 1647 609 87
Fragments arranged by size:
1647 609 87
FseI GG_CCGG'CC
Cuts at: 0 600 2343
Size: 600 1743
FspI TGC'GCA
Cuts at: 0 631 2343
Size: 631 1712
HaeII r_GCGC'y
Cuts at: 0 74 460 2343
Size: 74 386 1883
Fragments arranged by size:
1883 386 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 838 870 904 936 1384 1416 2011 2043
Size: 838 32 34 32 448 32 595 32
Cuts at: 2043 2212 2244 2343
Size: 169 32 99
Fragments arranged by size:
838 595 448 169 99 34 32 32
32 32 32
HindIII A'AGCT_T
Cuts at: 0 1056 2343
Size: 1056 1287
KpnI G_GTAC'C
Cuts at: 0 1066 2343
Size: 1066 1277
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1870 2343
Size: 1870 473
MscI TGG'CCA
Cuts at: 0 769 2343
Size: 769 1574
MslI CAynn'nnrTG
Cuts at: 0 1354 2343
Size: 1354 989
MspA1I CmG'CkG
Cuts at: 0 181 315 540 593 603 1518 2074 2319
Size: 181 134 225 53 10 915 556 245
Cuts at: 2319 2343
Size: 24
Fragments arranged by size:
915 556 245 225 181 134 53 24
10
NaeI GCC'GGC
Cuts at: 0 598 823 2343
Size: 598 225 1520
Fragments arranged by size:
1520 598 225
NdeI CA'TA_TG
Cuts at: 0 980 2343
Size: 980 1363
NgoMIV G'CCGG_C
Cuts at: 0 596 821 2343
Size: 596 225 1522
Fragments arranged by size:
1522 596 225
PpuMI rG'GwC_Cy
Cuts at: 0 1114 2343
Size: 1114 1229
PshAI GACnn'nnGTC
Cuts at: 0 1963 2343
Size: 1963 380
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1652 1684 2343
Size: 1652 32 659
Fragments arranged by size:
1652 659 32
PstI C_TGCA'G
Cuts at: 0 362 548 1517 2343
Size: 362 186 969 826
Fragments arranged by size:
969 826 362 186
PvuII CAG'CTG
Cuts at: 0 1518 2074 2319 2343
Size: 1518 556 245 24
Fragments arranged by size:
1518 556 245 24
SacI G_AGCT'C
Cuts at: 0 494 722 1639 2343
Size: 494 228 917 704
Fragments arranged by size:
917 704 494 228
SacII CC_GC'GG
Cuts at: 0 594 2343
Size: 594 1749
SanDI GG'GwC_CC
Cuts at: 0 1114 2343
Size: 1114 1229
SapI GCTCTTCn'nnn_
Cuts at: 0 1038 2343
Size: 1038 1305
ScaI AGT'ACT
Cuts at: 0 1074 2343
Size: 1074 1269
SfcI C'TryA_G
Cuts at: 0 358 495 544 1305 1403 1513 1688 2069
Size: 358 137 49 761 98 110 175 381
Cuts at: 2069 2343
Size: 274
Fragments arranged by size:
761 381 358 274 175 137 110 98
49
SgrAI Cr'CCGG_yG
Cuts at: 0 1935 2343
Size: 1935 408
SmlI C'TyrA_G
Cuts at: 0 505 712 1566 1632 2343
Size: 505 207 854 66 711
Fragments arranged by size:
854 711 505 207 66
StuI AGG'CCT
Cuts at: 0 1337 2343
Size: 1337 1006
StyI C'CwwG_G
Cuts at: 0 2052 2343
Size: 2052 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 808 944 2343
Size: 808 136 1399
Fragments arranged by size:
1399 808 136
TatI w'GTAC_w
Cuts at: 0 1072 2106 2343
Size: 1072 1034 237
Fragments arranged by size:
1072 1034 237
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 919 951 1070 1102 1130 1162 2343
Size: 919 32 119 32 28 32 1181
Fragments arranged by size:
1181 919 119 32 32 32 28
Tth111I GACn'n_nGTC
Cuts at: 0 334 852 2343
Size: 334 518 1491
Fragments arranged by size:
1491 518 334
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1486 2343
Size: 1486 857
XhoI C'TCGA_G
Cuts at: 0 505 2343
Size: 505 1838
XmnI GAAnn'nnTTC
Cuts at: 0 1258 1370 2063 2343
Size: 1258 112 693 280
Fragments arranged by size:
1258 693 280 112
Enzymes that do cut:
AccI Acc65I AcuI AgeI AhdI AleI AlwNI ApoI
AvaI BanI BanII BbsI BbvCI BglI BlpI BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstEII BstXI BstYI BstZ17I BtgI BtsI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI HaeII Hin4I HindIII KpnI MmeI
MscI MslI MspA1I NaeI NdeI NgoMIV PpuMI PshAI
PsrI PstI PvuII SacI SacII SanDI SapI ScaI
SfcI SgrAI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BcgI BciVI BclI BfrBI BglII Bme1580I BmtI
BplI BsaBI BsiWI BspHI BsrGI BstAPI BstBI Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI FspAI HincII
HpaI KasI MfeI MluI NarI NcoI NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PsiI PspOMI PspXI PvuI RsrII
SalI SbfI SexAI SfiI SfoI SgfI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XmaI ZraI