Restriction map for
pFN21AB6273
(Linear) (Six-base) MAPSORT of: KIBB6273.seq Check: 7959 from: 1 to: 2337
KIBB6273.orf
With 182 enzymes: SgfI *
February 5, 2010 16:50 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 655 1581 1804 2337
Size: 655 926 223 533
Fragments arranged by size:
926 655 533 223
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 932 2337
Size: 176 756 1405
Fragments arranged by size:
1405 756 176
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 663 2337
Size: 663 1674
ApaI G_GGCC'C
Cuts at: 0 1668 2337
Size: 1668 669
ApoI r'AATT_y
Cuts at: 0 845 2332 2337
Size: 845 1487 5
Fragments arranged by size:
1487 845 5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2188 2221 2337
Size: 2188 33 116
Fragments arranged by size:
2188 116 33
BanI G'GyrC_C
Cuts at: 0 1714 2337
Size: 1714 623
BanII G_rGCy'C
Cuts at: 0 1654 1668 2337
Size: 1654 14 669
Fragments arranged by size:
1654 669 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 575 1346 2337
Size: 575 771 991
Fragments arranged by size:
991 771 575
BbvCI CC'TCA_GC
Cuts at: 0 776 781 1949 2147 2337
Size: 776 5 1168 198 190
Fragments arranged by size:
1168 776 198 190 5
BclI T'GATC_A
Cuts at: 0 1164 2337
Size: 1164 1173
BglII A'GATC_T
Cuts at: 0 750 2337
Size: 750 1587
BlpI GC'TnA_GC
Cuts at: 0 1287 2036 2258 2337
Size: 1287 749 222 79
Fragments arranged by size:
1287 749 222 79
Bme1580I G_kGCm'C
Cuts at: 0 57 1668 2337
Size: 57 1611 669
Fragments arranged by size:
1611 669 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 1781 1923 2337
Size: 1781 142 414
Fragments arranged by size:
1781 414 142
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1263 1295 1541 1852 2084 2337
Size: 1263 32 246 311 232 253
Fragments arranged by size:
1263 311 253 246 232 32
Bpu10I CC'TnA_GC
Cuts at: 0 271 776 781 1413 1741 1949 2132 2147
Size: 271 505 5 632 328 208 183 15
Cuts at: 2147 2337
Size: 190
Fragments arranged by size:
632 505 328 271 208 190 183 15
5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1392 1793 2337
Size: 604 788 401 544
Fragments arranged by size:
788 604 544 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 57 292 2337
Size: 57 235 2045
Fragments arranged by size:
2045 235 57
BsaAI yAC'GTr
Cuts at: 0 436 2337
Size: 436 1901
BsaBI GATnn'nnATC
Cuts at: 0 1535 2337
Size: 1535 802
BsaWI w'CCGG_w
Cuts at: 0 1257 2337
Size: 1257 1080
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 876 906 1470 1500 2337
Size: 876 30 564 30 837
Fragments arranged by size:
876 837 564 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 764 1472 1475 2157 2337
Size: 761 3 708 3 682 180
Fragments arranged by size:
761 708 682 180 3 3
BseYI C'CCAG_C
Cuts at: 0 1698 2337
Size: 1698 639
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 633 2337
Size: 53 580 1704
Fragments arranged by size:
1704 580 53
Bsp1286I G_dGCh'C
Cuts at: 0 57 1654 1668 2337
Size: 57 1597 14 669
Fragments arranged by size:
1597 669 57 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 655 814 1581 1804 2337
Size: 655 159 767 223 533
Fragments arranged by size:
767 655 533 223 159
BsrBI CCG'CTC
Cuts at: 0 1745 2337
Size: 1745 592
BsrDI GCAATG_nn'
Cuts at: 0 408 1799 2337
Size: 408 1391 538
Fragments arranged by size:
1391 538 408
BsrGI T'GTAC_A
Cuts at: 0 528 2337
Size: 528 1809
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1621 2337
Size: 1621 716
BstEII G'GTnAC_C
Cuts at: 0 472 2337
Size: 472 1865
BstXI CCAn_nnnn'nTGG
Cuts at: 0 980 2337
Size: 980 1357
BstYI r'GATC_y
Cuts at: 0 727 750 1077 1530 2337
Size: 727 23 327 453 807
Fragments arranged by size:
807 727 453 327 23
Bsu36I CC'TnA_GG
Cuts at: 0 1428 1758 2337
Size: 1428 330 579
Fragments arranged by size:
1428 579 330
BtgI C'CryG_G
Cuts at: 0 1595 2337
Size: 1595 742
BtsI GCAGTG_nn'
Cuts at: 0 650 1780 2337
Size: 650 1130 557
Fragments arranged by size:
1130 650 557
DraI TTT'AAA
Cuts at: 0 1832 2337
Size: 1832 505
EaeI y'GGCC_r
Cuts at: 0 899 982 1598 2337
Size: 899 83 616 739
Fragments arranged by size:
899 739 616 83
EarI CTCTTCn'nnn_
Cuts at: 0 2302 2337
Size: 2302 35
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 932 1263 1295 1541 1852 2084 2337
Size: 176 756 331 32 246 311 232 253
Fragments arranged by size:
756 331 311 253 246 232 176 32
EcoO109I rG'GnC_Cy
Cuts at: 0 1664 1853 2337
Size: 1664 189 484
Fragments arranged by size:
1664 484 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 592 2337
Size: 592 1745
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 202 234 675 707 2147 2179 2337
Size: 202 32 441 32 1440 32 158
Fragments arranged by size:
1440 441 202 158 32 32 32
HindIII A'AGCT_T
Cuts at: 0 8 196 2337
Size: 8 188 2141
Fragments arranged by size:
2141 188 8
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1501 2337
Size: 526 975 836
Fragments arranged by size:
975 836 526
MscI TGG'CCA
Cuts at: 0 901 984 2337
Size: 901 83 1353
Fragments arranged by size:
1353 901 83
MspA1I CmG'CkG
Cuts at: 0 610 780 1561 2095 2337
Size: 610 170 781 534 242
Fragments arranged by size:
781 610 534 242 170
NspI r_CATG'y
Cuts at: 0 1551 2337
Size: 1551 786
PflMI CCAn_nnn'nTGG
Cuts at: 0 1611 2337
Size: 1611 726
PspOMI G'GGCC_C
Cuts at: 0 1664 2337
Size: 1664 673
PstI C_TGCA'G
Cuts at: 0 1731 1907 2337
Size: 1731 176 430
Fragments arranged by size:
1731 430 176
PvuII CAG'CTG
Cuts at: 0 610 780 1561 2095 2337
Size: 610 170 781 534 242
Fragments arranged by size:
781 610 534 242 170
SbfI CC_TGCA'GG
Cuts at: 0 1731 2337
Size: 1731 606
SfcI C'TryA_G
Cuts at: 0 441 605 635 1727 1903 2337
Size: 441 164 30 1092 176 434
Fragments arranged by size:
1092 441 434 176 164 30
SmlI C'TyrA_G
Cuts at: 0 583 1407 1808 2337
Size: 583 824 401 529
Fragments arranged by size:
824 583 529 401
SphI G_CATG'C
Cuts at: 0 1551 2337
Size: 1551 786
SspI AAT'ATT
Cuts at: 0 340 402 2337
Size: 340 62 1935
Fragments arranged by size:
1935 340 62
StuI AGG'CCT
Cuts at: 0 2026 2337
Size: 2026 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1213 1618 2337
Size: 1213 405 719
Fragments arranged by size:
1213 719 405
TatI w'GTAC_w
Cuts at: 0 528 2337
Size: 528 1809
Tth111I GACn'n_nGTC
Cuts at: 0 216 2337
Size: 216 2121
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1608 1876 2337
Size: 1608 268 461
Fragments arranged by size:
1608 461 268
XmnI GAAnn'nnTTC
Cuts at: 0 1422 2337
Size: 1422 915
Enzymes that do cut:
AarI AcuI AlfI ApaI ApoI BaeI BanI BanII
BbsI BbvCI BclI BglII BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI
BseYI BsgI Bsp1286I BspMI BsrBI BsrDI BsrGI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtsI DraI EaeI
EarI Eco57MI EcoO109I FalI Hin4I HindIII MmeI MscI
MspA1I NspI PflMI PspOMI PstI PvuII SbfI SfcI
SmlI SphI SspI StuI TaqII TatI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AlwNI ApaLI AscI AseI AsiSI
AvaI AvrII BamHI BbeI BcgI BciVI BfrBI BglI
BmgBI BmtI BplI BsaHI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrFI BssHII BssSI BstBI BstZ17I
BtgZI ClaI CspCI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI StyI SwaI TstI XbaI
XhoI XmaI ZraI