Restriction map for
pFN21AB6247
(Linear) (Six-base) MAPSORT of: KIBB6247.seq Check: 2285 from: 1 to: 2262
KIBB6247.orf
With 182 enzymes: SgfI *
July 3, 2012 16:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 802 2262
Size: 802 1460
AccI GT'mk_AC
Cuts at: 0 713 2262
Size: 713 1549
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 153 1242 1662 2262
Size: 153 1089 420 600
Fragments arranged by size:
1089 600 420 153
AhdI GACnn_n'nnGTC
Cuts at: 0 1540 2262
Size: 1540 722
AleI CACnn'nnGTG
Cuts at: 0 407 1889 1973 2262
Size: 407 1482 84 289
Fragments arranged by size:
1482 407 289 84
AlwNI CAG_nnn'CTG
Cuts at: 0 279 306 529 931 1545 2262
Size: 279 27 223 402 614 717
Fragments arranged by size:
717 614 402 279 223 27
ApaLI G'TGCA_C
Cuts at: 0 259 2262
Size: 259 2003
ApoI r'AATT_y
Cuts at: 0 21 856 1796 1964 2262
Size: 21 835 940 168 298
Fragments arranged by size:
940 835 298 168 21
BanI G'GyrC_C
Cuts at: 0 68 1871 2262
Size: 68 1803 391
Fragments arranged by size:
1803 391 68
BanII G_rGCy'C
Cuts at: 0 233 329 523 537 762 2262
Size: 233 96 194 14 225 1500
Fragments arranged by size:
1500 233 225 194 96 14
BbeI G_GCGC'C
Cuts at: 0 72 2262
Size: 72 2190
BbsI GAAGACnn'nnnn_
Cuts at: 0 1091 1258 2262
Size: 1091 167 1004
Fragments arranged by size:
1091 1004 167
BciVI GTATCCnnnnn_n'
Cuts at: 0 678 2262
Size: 678 1584
BfrBI ATG'CAT
Cuts at: 0 453 2262
Size: 453 1809
BglI GCCn_nnn'nGGC
Cuts at: 0 808 2262
Size: 808 1454
BlpI GC'TnA_GC
Cuts at: 0 522 2262
Size: 522 1740
Bme1580I G_kGCm'C
Cuts at: 0 263 2262
Size: 263 1999
BmrI ACTGGGnnnn_n'
Cuts at: 0 572 1478 1646 1730 2066 2262
Size: 572 906 168 84 336 196
Fragments arranged by size:
906 572 336 196 168 84
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2253 2262
Size: 2253 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 301 551 562 590 953 1238 1826
Size: 109 192 250 11 28 363 285 588
Cuts at: 1826 2010 2246 2262
Size: 184 236 16
Fragments arranged by size:
588 363 285 250 236 192 184 109
28 16 11
Bpu10I CC'TnA_GC
Cuts at: 0 307 2262
Size: 307 1955
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 608 2262
Size: 316 292 1654
Fragments arranged by size:
1654 316 292
BsaI GGTCTCn'nnnn_
Cuts at: 0 635 2262
Size: 635 1627
BsaHI Gr'CG_yC
Cuts at: 0 69 2262
Size: 69 2193
BsaWI w'CCGG_w
Cuts at: 0 329 2140 2262
Size: 329 1811 122
Fragments arranged by size:
1811 329 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 557 587 1677 1707 2262
Size: 557 30 1090 30 555
Fragments arranged by size:
1090 557 555 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 382 433 475 485 2262
Size: 382 51 42 10 1777
Fragments arranged by size:
1777 382 51 42 10
BseYI C'CCAG_C
Cuts at: 0 432 1271 2262
Size: 432 839 991
Fragments arranged by size:
991 839 432
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 1531 2262
Size: 361 1170 731
Fragments arranged by size:
1170 731 361
BsiHKAI G_wGCw'C
Cuts at: 0 46 233 263 329 2262
Size: 46 187 30 66 1933
Fragments arranged by size:
1933 187 66 46 30
BsmI GAATG_Cn'
Cuts at: 0 1324 1492 1507 1591 2262
Size: 1324 168 15 84 671
Fragments arranged by size:
1324 671 168 84 15
Bsp1286I G_dGCh'C
Cuts at: 0 46 233 263 329 523 537 762 2262
Size: 46 187 30 66 194 14 225 1500
Fragments arranged by size:
1500 225 194 187 66 46 30 14
BspEI T'CCGG_A
Cuts at: 0 329 2262
Size: 329 1933
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 802 2262
Size: 802 1460
BsrDI GCAATG_nn'
Cuts at: 0 1497 1581 1917 2085 2262
Size: 1497 84 336 168 177
Fragments arranged by size:
1497 336 177 168 84
BsrFI r'CCGG_y
Cuts at: 0 218 2262
Size: 218 2044
BsrGI T'GTAC_A
Cuts at: 0 2121 2262
Size: 2121 141
BssSI C'ACGA_G
Cuts at: 0 187 2262
Size: 187 2075
BstBI TT'CG_AA
Cuts at: 0 1515 2262
Size: 1515 747
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2034 2262
Size: 2034 228
BstYI r'GATC_y
Cuts at: 0 275 2262
Size: 275 1987
Bsu36I CC'TnA_GG
Cuts at: 0 2238 2262
Size: 2238 24
BtsI GCAGTG_nn'
Cuts at: 0 5 44 772 1329 2262
Size: 5 39 728 557 933
Fragments arranged by size:
933 728 557 39 5
ClaI AT'CG_AT
Cuts at: 0 1149 2262
Size: 1149 1113
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1479 1514 1563 1598 1899 1934 2067 2102
Size: 1479 35 49 35 301 35 133 35
Cuts at: 2102 2262
Size: 160
Fragments arranged by size:
1479 301 160 133 49 35 35 35
35
DraI TTT'AAA
Cuts at: 0 864 1130 2262
Size: 864 266 1132
Fragments arranged by size:
1132 864 266
EaeI y'GGCC_r
Cuts at: 0 1197 2262
Size: 1197 1065
EcoICRI GAG'CTC
Cuts at: 0 231 327 2262
Size: 231 96 1935
Fragments arranged by size:
1935 231 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 153 301 551 562 590 953 1238
Size: 109 44 148 250 11 28 363 285
Cuts at: 1238 1242 1662 1826 2010 2246 2262
Size: 4 420 164 184 236 16
Fragments arranged by size:
420 363 285 250 236 184 164 148
109 44 28 16 11 4
EcoO109I rG'GnC_Cy
Cuts at: 0 677 702 1542 2262
Size: 677 25 840 720
Fragments arranged by size:
840 720 677 25
EcoRI G'AATT_C
Cuts at: 0 1796 1964 2262
Size: 1796 168 298
Fragments arranged by size:
1796 298 168
HaeII r_GCGC'y
Cuts at: 0 72 2262
Size: 72 2190
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 456 488 691 723 1526 1558 1677 1709
Size: 456 32 203 32 803 32 119 32
Cuts at: 1709 1845 1877 2262
Size: 136 32 385
Fragments arranged by size:
803 456 385 203 136 119 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 842 2262
Size: 842 1420
HindIII A'AGCT_T
Cuts at: 0 1169 2093 2262
Size: 1169 924 169
Fragments arranged by size:
1169 924 169
KasI G'GCGC_C
Cuts at: 0 68 2262
Size: 68 2194
MfeI C'AATT_G
Cuts at: 0 175 2262
Size: 175 2087
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1663 2262
Size: 1663 599
MscI TGG'CCA
Cuts at: 0 1199 2262
Size: 1199 1063
MslI CAynn'nnrTG
Cuts at: 0 407 1737 1889 1973 2262
Size: 407 1330 152 84 289
Fragments arranged by size:
1330 407 289 152 84
MspA1I CmG'CkG
Cuts at: 0 239 345 2262
Size: 239 106 1917
Fragments arranged by size:
1917 239 106
NaeI GCC'GGC
Cuts at: 0 220 2262
Size: 220 2042
NarI GG'CG_CC
Cuts at: 0 69 2262
Size: 69 2193
NdeI CA'TA_TG
Cuts at: 0 696 2262
Size: 696 1566
NgoMIV G'CCGG_C
Cuts at: 0 218 2262
Size: 218 2044
NsiI A_TGCA'T
Cuts at: 0 455 2262
Size: 455 1807
PasI CC'CwG_GG
Cuts at: 0 319 559 2262
Size: 319 240 1703
Fragments arranged by size:
1703 319 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 798 2262
Size: 798 1464
PfoI T'CCnGG_A
Cuts at: 0 278 2262
Size: 278 1984
PpuMI rG'GwC_Cy
Cuts at: 0 702 1542 2262
Size: 702 840 720
Fragments arranged by size:
840 720 702
PstI C_TGCA'G
Cuts at: 0 12 767 2262
Size: 12 755 1495
Fragments arranged by size:
1495 755 12
PvuII CAG'CTG
Cuts at: 0 345 2262
Size: 345 1917
SacI G_AGCT'C
Cuts at: 0 233 329 2262
Size: 233 96 1933
Fragments arranged by size:
1933 233 96
SfcI C'TryA_G
Cuts at: 0 8 710 763 1341 2262
Size: 8 702 53 578 921
Fragments arranged by size:
921 702 578 53 8
SfoI GGC'GCC
Cuts at: 0 70 2262
Size: 70 2192
SmlI C'TyrA_G
Cuts at: 0 295 623 2262
Size: 295 328 1639
Fragments arranged by size:
1639 328 295
StuI AGG'CCT
Cuts at: 0 29 1339 2262
Size: 29 1310 923
Fragments arranged by size:
1310 923 29
StyI C'CwwG_G
Cuts at: 0 266 806 2262
Size: 266 540 1456
Fragments arranged by size:
1456 540 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 327 2262
Size: 327 1935
TatI w'GTAC_w
Cuts at: 0 598 660 2121 2262
Size: 598 62 1461 141
Fragments arranged by size:
1461 598 141 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 557 589 2262
Size: 557 32 1673
Fragments arranged by size:
1673 557 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 193 577 2262
Size: 193 384 1685
Fragments arranged by size:
1685 384 193
XmnI GAAnn'nnTTC
Cuts at: 0 1255 1759 2011 2262
Size: 1255 504 252 251
Fragments arranged by size:
1255 504 252 251
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlwNI ApaLI ApoI
BanI BanII BbeI BbsI BciVI BfrBI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspMI BsrDI BsrFI BsrGI BssSI BstBI BstXI
BstYI Bsu36I BtsI ClaI CspCI DraI EaeI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII HindIII KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NdeI
NgoMIV NsiI PasI PflMI PfoI PpuMI PstI PvuII
SacI SfcI SfoI SmlI StuI StyI TaqII TatI
TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AlfI
AloI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BclI BglII BmgBI BmtI BsaAI
BsaBI BsiEI BsiWI BsmBI BspHI BsrBI BssHII BstAPI
BstEII BstZ17I BtgI BtgZI DraIII DrdI EagI EarI
EciI EcoNI EcoRV FalI FseI FspI FspAI HpaI
KpnI MluI NcoI NheI NotI NruI NspI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XhoI XmaI
ZraI