(Linear) (Six-base) MAPSORT of: KIBB6244.seq Check: 3800 from: 1 to: 2040 KIBB6244.orf With 182 enzymes: SgfI * July 3, 2012 16:16 .. AccI GT'mk_AC Cuts at: 0 689 2040 Size: 689 1351 Acc65I G'GTAC_C Cuts at: 0 861 2040 Size: 861 1179 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 1324 2040 Size: 613 711 716 Fragments arranged by size: 716 711 613 AflIII A'CryG_T Cuts at: 0 533 2040 Size: 533 1507 AleI CACnn'nnGTG Cuts at: 0 715 2040 Size: 715 1325 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 639 1543 2040 Size: 639 904 497 Fragments arranged by size: 904 639 497 AlwNI CAG_nnn'CTG Cuts at: 0 1707 2040 Size: 1707 333 ApoI r'AATT_y Cuts at: 0 3 142 754 892 1825 2040 Size: 3 139 612 138 933 215 Fragments arranged by size: 933 612 215 139 138 3 AvaI C'yCGr_G Cuts at: 0 1898 1961 2040 Size: 1898 63 79 Fragments arranged by size: 1898 79 63 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 1609 1642 2040 Size: 1609 33 398 Fragments arranged by size: 1609 398 33 BanI G'GyrC_C Cuts at: 0 432 728 861 1519 2040 Size: 432 296 133 658 521 Fragments arranged by size: 658 521 432 296 133 BanII G_rGCy'C Cuts at: 0 347 1888 2040 Size: 347 1541 152 Fragments arranged by size: 1541 347 152 BbeI G_GCGC'C Cuts at: 0 436 2040 Size: 436 1604 BbsI GAAGACnn'nnnn_ Cuts at: 0 852 2040 Size: 852 1188 BbvCI CC'TCA_GC Cuts at: 0 1283 1451 2040 Size: 1283 168 589 Fragments arranged by size: 1283 589 168 BciVI GTATCCnnnnn_n' Cuts at: 0 295 1162 1637 2040 Size: 295 867 475 403 Fragments arranged by size: 867 475 403 295 BglI GCCn_nnn'nGGC Cuts at: 0 565 2040 Size: 565 1475 BlpI GC'TnA_GC Cuts at: 0 701 745 2040 Size: 701 44 1295 Fragments arranged by size: 1295 701 44 Bme1580I G_kGCm'C Cuts at: 0 733 1522 2040 Size: 733 789 518 Fragments arranged by size: 789 733 518 BmgBI CAC'GTC Cuts at: 0 534 708 2040 Size: 534 174 1332 Fragments arranged by size: 1332 534 174 BmrI ACTGGGnnnn_n' Cuts at: 0 813 2040 Size: 813 1227 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1752 2040 Size: 1752 288 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1559 1872 2040 Size: 1559 313 168 Fragments arranged by size: 1559 313 168 Bpu10I CC'TnA_GC Cuts at: 0 1283 1451 1769 2040 Size: 1283 168 318 271 Fragments arranged by size: 1283 318 271 168 BsaI GGTCTCn'nnnn_ Cuts at: 0 1888 2040 Size: 1888 152 BsaHI Gr'CG_yC Cuts at: 0 433 2040 Size: 433 1607 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 639 669 1813 1843 2040 Size: 639 30 1144 30 197 Fragments arranged by size: 1144 639 197 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 372 647 1821 2040 Size: 372 275 1174 219 Fragments arranged by size: 1174 372 275 219 BseYI C'CCAG_C Cuts at: 0 75 454 2040 Size: 75 379 1586 Fragments arranged by size: 1586 379 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1593 2040 Size: 1593 447 BsiHKAI G_wGCw'C Cuts at: 0 130 556 707 2040 Size: 130 426 151 1333 Fragments arranged by size: 1333 426 151 130 Bsp1286I G_dGCh'C Cuts at: 0 130 347 556 707 733 1522 1888 2040 Size: 130 217 209 151 26 789 366 152 Fragments arranged by size: 789 366 217 209 152 151 130 26 BsrFI r'CCGG_y Cuts at: 0 1811 2040 Size: 1811 229 BsrGI T'GTAC_A Cuts at: 0 908 2040 Size: 908 1132 BstYI r'GATC_y Cuts at: 0 831 1028 1180 2040 Size: 831 197 152 860 Fragments arranged by size: 860 831 197 152 Bsu36I CC'TnA_GG Cuts at: 0 659 2040 Size: 659 1381 BtgI C'CryG_G Cuts at: 0 1739 2040 Size: 1739 301 BtsI GCAGTG_nn' Cuts at: 0 1605 1869 2040 Size: 1605 264 171 Fragments arranged by size: 1605 264 171 DraIII CAC_nnn'GTG Cuts at: 0 22 2040 Size: 22 2018 EaeI y'GGCC_r Cuts at: 0 322 566 2040 Size: 322 244 1474 Fragments arranged by size: 1474 322 244 EarI CTCTTCn'nnn_ Cuts at: 0 1041 2040 Size: 1041 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1075 1927 2040 Size: 1075 852 113 Fragments arranged by size: 1075 852 113 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 613 1324 1559 1872 2040 Size: 613 711 235 313 168 Fragments arranged by size: 711 613 313 235 168 EcoRI G'AATT_C Cuts at: 0 142 754 892 1825 2040 Size: 142 612 138 933 215 Fragments arranged by size: 933 612 215 142 138 HaeII r_GCGC'y Cuts at: 0 436 1128 2040 Size: 436 692 912 Fragments arranged by size: 912 692 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 223 255 460 492 639 656 671 688 Size: 223 32 205 32 147 17 15 17 Cuts at: 688 762 794 1772 1804 1919 1951 2040 Size: 74 32 978 32 115 32 89 Fragments arranged by size: 978 223 205 147 115 89 74 32 32 32 32 32 17 17 15 KasI G'GCGC_C Cuts at: 0 432 2040 Size: 432 1608 KpnI G_GTAC'C Cuts at: 0 865 2040 Size: 865 1175 MfeI C'AATT_G Cuts at: 0 1292 2040 Size: 1292 748 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 524 918 2040 Size: 524 394 1122 Fragments arranged by size: 1122 524 394 MscI TGG'CCA Cuts at: 0 324 2040 Size: 324 1716 MslI CAynn'nnrTG Cuts at: 0 715 1510 1525 1531 2040 Size: 715 795 15 6 509 Fragments arranged by size: 795 715 509 15 6 NarI GG'CG_CC Cuts at: 0 433 2040 Size: 433 1607 NcoI C'CATG_G Cuts at: 0 1739 2040 Size: 1739 301 NdeI CA'TA_TG Cuts at: 0 1374 2040 Size: 1374 666 NspI r_CATG'y Cuts at: 0 294 626 1530 1786 2040 Size: 294 332 904 256 254 Fragments arranged by size: 904 332 294 256 254 PasI CC'CwG_GG Cuts at: 0 1701 2040 Size: 1701 339 PflMI CCAn_nnn'nTGG Cuts at: 0 1537 2040 Size: 1537 503 PfoI T'CCnGG_A Cuts at: 0 1802 2040 Size: 1802 238 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 367 399 2040 Size: 367 32 1641 Fragments arranged by size: 1641 367 32 ScaI AGT'ACT Cuts at: 0 37 2040 Size: 37 2003 SfcI C'TryA_G Cuts at: 0 31 1871 2040 Size: 31 1840 169 Fragments arranged by size: 1840 169 31 SfoI GGC'GCC Cuts at: 0 434 2040 Size: 434 1606 SmaI CCC'GGG Cuts at: 0 1900 2040 Size: 1900 140 SphI G_CATG'C Cuts at: 0 294 2040 Size: 294 1746 StyI C'CwwG_G Cuts at: 0 1739 2040 Size: 1739 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1537 1579 2040 Size: 1537 42 461 Fragments arranged by size: 1537 461 42 TatI w'GTAC_w Cuts at: 0 35 329 908 1099 2040 Size: 35 294 579 191 941 Fragments arranged by size: 941 579 294 191 35 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 476 508 1136 1168 1813 1845 2040 Size: 476 32 628 32 645 32 195 Fragments arranged by size: 645 628 476 195 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1746 2040 Size: 1746 294 XmaI C'CCGG_G Cuts at: 0 1898 2040 Size: 1898 142 XmnI GAAnn'nnTTC Cuts at: 0 1978 2040 Size: 1978 62 Enzymes that do cut: AccI Acc65I AcuI AflIII AleI AlfI AlwNI ApoI AvaI BaeI BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI BsrGI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI EciI Eco57MI EcoRI HaeII Hin4I KasI KpnI MfeI MmeI MscI MslI NarI NcoI NdeI NspI PasI PflMI PfoI PpiI ScaI SfcI SfoI SmaI SphI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AclI AfeI AflII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BamHI BcgI BclI BfrBI BglII BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HincII HindIII HpaI MluI MspA1I NaeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI