Restriction map for
pFN21AB6231
(Linear) (Six-base) MAPSORT of: KIBB6231.seq Check: 5426 from: 1 to: 2229
KIBB6231.orf
With 182 enzymes: SgfI *
March 22, 2012 15:33 ..
AflIII A'CryG_T
Cuts at: 0 1814 2229
Size: 1814 415
AgeI A'CCGG_T
Cuts at: 0 1651 2229
Size: 1651 578
AhdI GACnn_n'nnGTC
Cuts at: 0 1290 2229
Size: 1290 939
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1978 2229
Size: 1978 251
AlwNI CAG_nnn'CTG
Cuts at: 0 1707 1773 2229
Size: 1707 66 456
Fragments arranged by size:
1707 456 66
ApaI G_GGCC'C
Cuts at: 0 248 1489 2006 2229
Size: 248 1241 517 223
Fragments arranged by size:
1241 517 248 223
ApaLI G'TGCA_C
Cuts at: 0 313 1867 2229
Size: 313 1554 362
Fragments arranged by size:
1554 362 313
ApoI r'AATT_y
Cuts at: 0 490 829 2229
Size: 490 339 1400
Fragments arranged by size:
1400 490 339
AvaI C'yCGr_G
Cuts at: 0 1159 1265 1419 1512 1659 2061 2229
Size: 1159 106 154 93 147 402 168
Fragments arranged by size:
1159 402 168 154 147 106 93
BanI G'GyrC_C
Cuts at: 0 72 172 358 1544 1585 1938 2229
Size: 72 100 186 1186 41 353 291
Fragments arranged by size:
1186 353 291 186 100 72 41
BanII G_rGCy'C
Cuts at: 0 248 284 1208 1369 1489 2006 2068 2229
Size: 248 36 924 161 120 517 62 161
Fragments arranged by size:
924 517 248 161 161 120 62 36
BbeI G_GCGC'C
Cuts at: 0 76 176 1589 2229
Size: 76 100 1413 640
Fragments arranged by size:
1413 640 100 76
BbsI GAAGACnn'nnnn_
Cuts at: 0 277 2229
Size: 277 1952
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2042 2076 2229
Size: 2042 34 153
Fragments arranged by size:
2042 153 34
BclI T'GATC_A
Cuts at: 0 675 2229
Size: 675 1554
BglI GCCn_nnn'nGGC
Cuts at: 0 1523 1696 1831 2029 2041 2050 2229
Size: 1523 173 135 198 12 9 179
Fragments arranged by size:
1523 198 179 173 135 12 9
BglII A'GATC_T
Cuts at: 0 1181 2229
Size: 1181 1048
BlpI GC'TnA_GC
Cuts at: 0 1256 2229
Size: 1256 973
Bme1580I G_kGCm'C
Cuts at: 0 248 317 361 1384 1489 1547 1871 2006
Size: 248 69 44 1023 105 58 324 135
Cuts at: 2006 2229
Size: 223
Fragments arranged by size:
1023 324 248 223 135 105 69 58
44
BmgBI CAC'GTC
Cuts at: 0 1196 1755 2229
Size: 1196 559 474
Fragments arranged by size:
1196 559 474
BmrI ACTGGGnnnn_n'
Cuts at: 0 587 864 2229
Size: 587 277 1365
Fragments arranged by size:
1365 587 277
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1002 1046 1578 1729 1842 2085 2229
Size: 340 662 44 532 151 113 243 144
Fragments arranged by size:
662 532 340 243 151 144 113 44
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 2229
Size: 211 2018
BsaAI yAC'GTr
Cuts at: 0 686 1817 2229
Size: 686 1131 412
Fragments arranged by size:
1131 686 412
BsaHI Gr'CG_yC
Cuts at: 0 73 173 1127 1586 1717 2229
Size: 73 100 954 459 131 512
Fragments arranged by size:
954 512 459 131 100 73
BsaWI w'CCGG_w
Cuts at: 0 21 1651 2229
Size: 21 1630 578
Fragments arranged by size:
1630 578 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 123 153 2068 2098 2229
Size: 123 30 1915 30 131
Fragments arranged by size:
1915 131 123 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 225 1162 1216 1369 1833 2076 2088 2229
Size: 225 937 54 153 464 243 12 141
Fragments arranged by size:
937 464 243 225 153 141 54 12
BseYI C'CCAG_C
Cuts at: 0 99 1108 1564 2229
Size: 99 1009 456 665
Fragments arranged by size:
1009 665 456 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1471 1543 2229
Size: 1471 72 686
Fragments arranged by size:
1471 686 72
BsiEI CG_ry'CG
Cuts at: 0 215 1466 1607 1838 1997 2229
Size: 215 1251 141 231 159 232
Fragments arranged by size:
1251 232 231 215 159 141
BsiHKAI G_wGCw'C
Cuts at: 0 317 1871 2229
Size: 317 1554 358
Fragments arranged by size:
1554 358 317
Bsp1286I G_dGCh'C
Cuts at: 0 248 284 317 361 1208 1369 1384 1489
Size: 248 36 33 44 847 161 15 105
Cuts at: 1489 1547 1871 2006 2068 2229
Size: 58 324 135 62 161
Fragments arranged by size:
847 324 248 161 161 135 105 62
58 44 36 33 15
BsrBI CCG'CTC
Cuts at: 0 1260 1443 1551 2084 2229
Size: 1260 183 108 533 145
Fragments arranged by size:
1260 533 183 145 108
BsrFI r'CCGG_y
Cuts at: 0 220 1389 1651 1941 2229
Size: 220 1169 262 290 288
Fragments arranged by size:
1169 290 288 262 220
BsrGI T'GTAC_A
Cuts at: 0 551 2229
Size: 551 1678
BssHII G'CGCG_C
Cuts at: 0 1408 1410 1412 2229
Size: 1408 2 2 817
Fragments arranged by size:
1408 817 2 2
BstEII G'GTnAC_C
Cuts at: 0 103 2229
Size: 103 2126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1680 2229
Size: 1680 549
BstYI r'GATC_y
Cuts at: 0 18 1181 2229
Size: 18 1163 1048
Fragments arranged by size:
1163 1048 18
BtgI C'CryG_G
Cuts at: 0 275 1229 1577 1631 1673 1679 1721 1906
Size: 275 954 348 54 42 6 42 185
Cuts at: 1906 1985 1997 2229
Size: 79 12 232
Fragments arranged by size:
954 348 275 232 185 79 54 42
42 12 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 819 2229
Size: 819 1410
BtsI GCAGTG_nn'
Cuts at: 0 2060 2229
Size: 2060 169
DraI TTT'AAA
Cuts at: 0 408 2229
Size: 408 1821
DrdI GACnn_nn'nnGTC
Cuts at: 0 2017 2229
Size: 2017 212
EaeI y'GGCC_r
Cuts at: 0 179 212 924 1175 1391 1463 1604 1628
Size: 179 33 712 251 216 72 141 24
Cuts at: 1628 1676 1724 1775 1823 1835 1994 2030 2147
Size: 48 48 51 48 12 159 36 117
Cuts at: 2147 2229
Size: 82
Fragments arranged by size:
712 251 216 179 159 141 117 82
72 51 48 48 48 36 33 24
12
EagI C'GGCC_G
Cuts at: 0 212 1463 1604 1835 1994 2229
Size: 212 1251 141 231 159 235
Fragments arranged by size:
1251 235 231 212 159 141
EarI CTCTTCn'nnn_
Cuts at: 0 284 924 1397 1558 2229
Size: 284 640 473 161 671
Fragments arranged by size:
671 640 473 284 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1002 1046 1578 1729 1842 2085 2229
Size: 340 662 44 532 151 113 243 144
Fragments arranged by size:
662 532 340 243 151 144 113 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1480 2229
Size: 1480 749
EcoO109I rG'GnC_Cy
Cuts at: 0 244 245 1001 1485 1486 1618 1643 1770
Size: 244 1 756 484 1 132 25 127
Cuts at: 1770 2002 2003 2210 2229
Size: 232 1 207 19
Fragments arranged by size:
756 484 244 232 207 132 127 25
19 1 1 1
EcoRI G'AATT_C
Cuts at: 0 829 2229
Size: 829 1400
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1449 2229
Size: 1449 780
HaeII r_GCGC'y
Cuts at: 0 76 160 176 1390 1589 2229
Size: 76 84 16 1214 199 640
Fragments arranged by size:
1214 640 199 84 76 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 8 40 123 155 180 212 1000 1032
Size: 8 32 83 32 25 32 788 32
Cuts at: 1032 1276 1308 2050 2082 2229
Size: 244 32 742 32 147
Fragments arranged by size:
788 742 244 147 83 32 32 32
32 32 32 25 8
KasI G'GCGC_C
Cuts at: 0 72 172 1585 2229
Size: 72 100 1413 644
Fragments arranged by size:
1413 644 100 72
MfeI C'AATT_G
Cuts at: 0 199 2229
Size: 199 2030
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1178 2229
Size: 1178 1051
MscI TGG'CCA
Cuts at: 0 181 1630 1777 2229
Size: 181 1449 147 452
Fragments arranged by size:
1449 452 181 147
MslI CAynn'nnrTG
Cuts at: 0 364 838 967 1678 1819 1936 2229
Size: 364 474 129 711 141 117 293
Fragments arranged by size:
711 474 364 293 141 129 117
MspA1I CmG'CkG
Cuts at: 0 1231 1274 1609 1693 1723 1831 1908 1999
Size: 1231 43 335 84 30 108 77 91
Cuts at: 1999 2175 2229
Size: 176 54
Fragments arranged by size:
1231 335 176 108 91 84 77 54
43 30
NaeI GCC'GGC
Cuts at: 0 1391 2229
Size: 1391 838
NarI GG'CG_CC
Cuts at: 0 73 173 1586 2229
Size: 73 100 1413 643
Fragments arranged by size:
1413 643 100 73
NcoI C'CATG_G
Cuts at: 0 275 1577 1679 1985 2229
Size: 275 1302 102 306 244
Fragments arranged by size:
1302 306 275 244 102
NgoMIV G'CCGG_C
Cuts at: 0 1389 2229
Size: 1389 840
NotI GC'GGCC_GC
Cuts at: 0 1463 1604 1835 1994 2229
Size: 1463 141 231 159 235
Fragments arranged by size:
1463 235 231 159 141
NruI TCG'CGA
Cuts at: 0 1376 2229
Size: 1376 853
PasI CC'CwG_GG
Cuts at: 0 240 1482 2229
Size: 240 1242 747
Fragments arranged by size:
1242 747 240
PflMI CCAn_nnn'nTGG
Cuts at: 0 2178 2229
Size: 2178 51
PfoI T'CCnGG_A
Cuts at: 0 908 1946 2229
Size: 908 1038 283
Fragments arranged by size:
1038 908 283
PpuMI rG'GwC_Cy
Cuts at: 0 1001 1618 2210 2229
Size: 1001 617 592 19
Fragments arranged by size:
1001 617 592 19
PsiI TTA'TAA
Cuts at: 0 488 2229
Size: 488 1741
PspOMI G'GGCC_C
Cuts at: 0 244 1485 2002 2229
Size: 244 1241 517 227
Fragments arranged by size:
1241 517 244 227
PstI C_TGCA'G
Cuts at: 0 959 2165 2229
Size: 959 1206 64
Fragments arranged by size:
1206 959 64
SacII CC_GC'GG
Cuts at: 0 1232 1724 1909 2000 2229
Size: 1232 492 185 91 229
Fragments arranged by size:
1232 492 229 185 91
SanDI GG'GwC_CC
Cuts at: 0 1618 2229
Size: 1618 611
SapI GCTCTTCn'nnn_
Cuts at: 0 1558 2229
Size: 1558 671
SfcI C'TryA_G
Cuts at: 0 955 1076 1917 2161 2229
Size: 955 121 841 244 68
Fragments arranged by size:
955 841 244 121 68
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1523 2229
Size: 1523 706
SfoI GGC'GCC
Cuts at: 0 74 174 1587 2229
Size: 74 100 1413 642
Fragments arranged by size:
1413 642 100 74
SgrAI Cr'CCGG_yG
Cuts at: 0 1651 2229
Size: 1651 578
SmaI CCC'GGG
Cuts at: 0 1267 1421 1514 2229
Size: 1267 154 93 715
Fragments arranged by size:
1267 715 154 93
StuI AGG'CCT
Cuts at: 0 264 1165 1432 2229
Size: 264 901 267 797
Fragments arranged by size:
901 797 267 264
StyI C'CwwG_G
Cuts at: 0 275 308 1394 1577 1679 1985 2229
Size: 275 33 1086 183 102 306 244
Fragments arranged by size:
1086 306 275 244 183 102 33
TatI w'GTAC_w
Cuts at: 0 551 796 2229
Size: 551 245 1433
Fragments arranged by size:
1433 551 245
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 189 315 2229
Size: 189 126 1914
Fragments arranged by size:
1914 189 126
XmaI C'CCGG_G
Cuts at: 0 1265 1419 1512 2229
Size: 1265 154 93 717
Fragments arranged by size:
1265 717 154 93
Enzymes that do cut:
AflIII AgeI AhdI AlfI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BpmI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BsrBI BsrFI BsrGI BssHII BstEII BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KasI
MfeI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NruI PasI PflMI PfoI PpuMI PsiI
PspOMI PstI SacII SanDI SapI SfcI SfiI SfoI
SgrAI SmaI StuI StyI TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AleI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BciVI BfrBI BmtI BplI Bpu10I BpuEI BsaBI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BssSI
BstAPI BstBI BstZ17I Bsu36I ClaI CspCI DraIII EciI
EcoICRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MluI NdeI NheI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PspXI PsrI PvuI PvuII
RsrII SacI SalI SbfI ScaI SexAI SgfI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI XmnI ZraI