Restriction map for
pFN21AB6166
(Linear) (Six-base) MAPSORT of: KIBB6166.seq Check: 3248 from: 1 to: 1809
KIBB6166.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 220 287 1809
Size: 220 67 1522
Fragments arranged by size:
1522 220 67
AatII G_ACGT'C
Cuts at: 0 369 1809
Size: 369 1440
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1506 1525 1715 1809
Size: 1506 19 190 94
Fragments arranged by size:
1506 190 94 19
AflII C'TTAA_G
Cuts at: 0 900 1809
Size: 900 909
AflIII A'CryG_T
Cuts at: 0 1116 1809
Size: 1116 693
ApaLI G'TGCA_C
Cuts at: 0 1168 1809
Size: 1168 641
ApoI r'AATT_y
Cuts at: 0 429 928 1567 1591 1809
Size: 429 499 639 24 218
Fragments arranged by size:
639 499 429 218 24
AseI AT'TA_AT
Cuts at: 0 470 1809
Size: 470 1339
BanII G_rGCy'C
Cuts at: 0 1024 1809
Size: 1024 785
BbsI GAAGACnn'nnnn_
Cuts at: 0 1363 1375 1809
Size: 1363 12 434
Fragments arranged by size:
1363 434 12
BciVI GTATCCnnnnn_n'
Cuts at: 0 1480 1809
Size: 1480 329
BclI T'GATC_A
Cuts at: 0 105 1809
Size: 105 1704
BglII A'GATC_T
Cuts at: 0 858 1809
Size: 858 951
Bme1580I G_kGCm'C
Cuts at: 0 1172 1224 1809
Size: 1172 52 585
Fragments arranged by size:
1172 585 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 83 1227 1311 1809
Size: 83 1144 84 498
Fragments arranged by size:
1144 498 84 83
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1206 1809
Size: 1206 603
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 287 761 1019 1082 1176 1809
Size: 203 84 474 258 63 94 633
Fragments arranged by size:
633 474 258 203 94 84 63
BsaI GGTCTCn'nnnn_
Cuts at: 0 1720 1809
Size: 1720 89
BsaHI Gr'CG_yC
Cuts at: 0 366 1809
Size: 366 1443
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 41 71 642 672 1809
Size: 41 30 571 30 1137
Fragments arranged by size:
1137 571 41 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1809
Size: 254 1555
BsiHKAI G_wGCw'C
Cuts at: 0 1009 1024 1172 1433 1809
Size: 1009 15 148 261 376
Fragments arranged by size:
1009 376 261 148 15
BsmI GAATG_Cn'
Cuts at: 0 1686 1809
Size: 1686 123
Bsp1286I G_dGCh'C
Cuts at: 0 1009 1024 1172 1224 1433 1809
Size: 1009 15 148 52 209 376
Fragments arranged by size:
1009 376 209 148 52 15
BspHI T'CATG_A
Cuts at: 0 722 1809
Size: 722 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 220 287 494 1809
Size: 220 67 207 1315
Fragments arranged by size:
1315 220 207 67
BsrDI GCAATG_nn'
Cuts at: 0 912 1809
Size: 912 897
BsrGI T'GTAC_A
Cuts at: 0 1047 1809
Size: 1047 762
BssSI C'ACGA_G
Cuts at: 0 578 1809
Size: 578 1231
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1013 1809
Size: 1013 796
BstYI r'GATC_y
Cuts at: 0 858 1809
Size: 858 951
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1739 1774 1809
Size: 1739 35 35
Fragments arranged by size:
1739 35 35
DraI TTT'AAA
Cuts at: 0 411 1809
Size: 411 1398
EaeI y'GGCC_r
Cuts at: 0 176 815 1809
Size: 176 639 994
Fragments arranged by size:
994 639 176
EarI CTCTTCn'nnn_
Cuts at: 0 1525 1809
Size: 1525 284
EcoICRI GAG'CTC
Cuts at: 0 1022 1809
Size: 1022 787
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 287 761 1019 1082 1176 1506 1525
Size: 203 84 474 258 63 94 330 19
Cuts at: 1525 1715 1809
Size: 190 94
Fragments arranged by size:
474 330 258 203 190 94 94 84
63 19
EcoO109I rG'GnC_Cy
Cuts at: 0 633 1809
Size: 633 1176
EcoRI G'AATT_C
Cuts at: 0 1591 1809
Size: 1591 218
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 226 1809
Size: 226 1583
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 343 375 901 933 1384 1416 1637 1669
Size: 343 32 526 32 451 32 221 32
Cuts at: 1669 1768 1800 1809
Size: 99 32 9
Fragments arranged by size:
526 451 343 221 99 32 32 32
32 32 9
HincII GTy'rAC
Cuts at: 0 237 695 1403 1809
Size: 237 458 708 406
Fragments arranged by size:
708 458 406 237
HindIII A'AGCT_T
Cuts at: 0 897 1280 1809
Size: 897 383 529
Fragments arranged by size:
897 529 383
MfeI C'AATT_G
Cuts at: 0 1365 1809
Size: 1365 444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 617 685 1809
Size: 617 68 1124
Fragments arranged by size:
1124 617 68
MscI TGG'CCA
Cuts at: 0 178 817 1809
Size: 178 639 992
Fragments arranged by size:
992 639 178
MspA1I CmG'CkG
Cuts at: 0 491 760 1809
Size: 491 269 1049
Fragments arranged by size:
1049 491 269
NspI r_CATG'y
Cuts at: 0 1120 1809
Size: 1120 689
PciI A'CATG_T
Cuts at: 0 1116 1809
Size: 1116 693
PfoI T'CCnGG_A
Cuts at: 0 195 996 1809
Size: 195 801 813
Fragments arranged by size:
813 801 195
PpuMI rG'GwC_Cy
Cuts at: 0 633 1809
Size: 633 1176
PsiI TTA'TAA
Cuts at: 0 1781 1809
Size: 1781 28
PstI C_TGCA'G
Cuts at: 0 284 505 1776 1809
Size: 284 221 1271 33
Fragments arranged by size:
1271 284 221 33
PvuII CAG'CTG
Cuts at: 0 760 1809
Size: 760 1049
SacI G_AGCT'C
Cuts at: 0 1024 1809
Size: 1024 785
ScaI AGT'ACT
Cuts at: 0 1517 1809
Size: 1517 292
SfcI C'TryA_G
Cuts at: 0 280 501 1444 1632 1772 1809
Size: 280 221 943 188 140 37
Fragments arranged by size:
943 280 221 188 140 37
SmlI C'TyrA_G
Cuts at: 0 900 1809
Size: 900 909
SspI AAT'ATT
Cuts at: 0 729 1809
Size: 729 1080
StyI C'CwwG_G
Cuts at: 0 117 309 1809
Size: 117 192 1500
Fragments arranged by size:
1500 192 117
TatI w'GTAC_w
Cuts at: 0 685 808 1047 1515 1809
Size: 685 123 239 468 294
Fragments arranged by size:
685 468 294 239 123
Tth111I GACn'n_nGTC
Cuts at: 0 544 1809
Size: 544 1265
ZraI GAC'GTC
Cuts at: 0 367 1809
Size: 367 1442
Enzymes that do cut:
AarI AatII AcuI AflII AflIII ApaLI ApoI AseI
BanII BbsI BciVI BclI BglII Bme1580I BmrI BplI
BpmI BsaI BsaHI BsaXI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BsrGI BssSI BstAPI BstYI CspCI
DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI FalI
Hin4I HincII HindIII MfeI MmeI MscI MspA1I NspI
PciI PfoI PpuMI PsiI PstI PvuII SacI ScaI
SfcI SmlI SspI StyI TatI Tth111I ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI AlwNI ApaI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BfrBI BglI BlpI
BmgBI BmtI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BssHII
BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI DraIII DrdI EagI EciI EcoNI EcoRV FseI
FspI FspAI HaeII HpaI KasI KpnI MluI MslI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
XbaI XcmI XhoI XmaI XmnI