Restriction map for
pFN21AB6165
(Linear) (Six-base) MAPSORT of: KIBB6165.seq Check: 4012 from: 1 to: 1944
KIBB6165.orf
With 182 enzymes: SgfI *
December 27, 2011 16:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1771 1944
Size: 1771 173
AatII G_ACGT'C
Cuts at: 0 32 401 1944
Size: 32 369 1543
Fragments arranged by size:
1543 369 32
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 120 255 1944
Size: 120 135 1689
Fragments arranged by size:
1689 135 120
AflII C'TTAA_G
Cuts at: 0 850 1944
Size: 850 1094
ApaI G_GGCC'C
Cuts at: 0 46 224 269 1041 1449 1754 1944
Size: 46 178 45 772 408 305 190
Fragments arranged by size:
772 408 305 190 178 46 45
ApoI r'AATT_y
Cuts at: 0 948 1944
Size: 948 996
AvaI C'yCGr_G
Cuts at: 0 223 1797 1944
Size: 223 1574 147
Fragments arranged by size:
1574 223 147
BanI G'GyrC_C
Cuts at: 0 87 768 791 1239 1430 1456 1944
Size: 87 681 23 448 191 26 488
Fragments arranged by size:
681 488 448 191 87 26 23
BanII G_rGCy'C
Cuts at: 0 46 224 269 551 742 1041 1180 1449
Size: 46 178 45 282 191 299 139 269
Cuts at: 1449 1754 1874 1944
Size: 305 120 70
Fragments arranged by size:
305 299 282 269 191 178 139 120
70 46 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 393 1944
Size: 393 1551
BbvCI CC'TCA_GC
Cuts at: 0 1912 1944
Size: 1912 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 305 1944
Size: 305 1639
BglI GCCn_nnn'nGGC
Cuts at: 0 101 251 273 1206 1428 1944
Size: 101 150 22 933 222 516
Fragments arranged by size:
933 516 222 150 101 22
BglII A'GATC_T
Cuts at: 0 887 1944
Size: 887 1057
Bme1580I G_kGCm'C
Cuts at: 0 46 224 269 794 1041 1337 1433 1449
Size: 46 178 45 525 247 296 96 16
Cuts at: 1449 1754 1821 1944
Size: 305 67 123
Fragments arranged by size:
525 305 296 247 178 123 96 67
46 45 16
BmgBI CAC'GTC
Cuts at: 0 492 1944
Size: 492 1452
BmrI ACTGGGnnnn_n'
Cuts at: 0 478 607 875 1831 1944
Size: 478 129 268 956 113
Fragments arranged by size:
956 478 268 129 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 152 484 640 1271 1888 1944
Size: 76 76 332 156 631 617 56
Fragments arranged by size:
631 617 332 156 76 76 56
Bpu10I CC'TnA_GC
Cuts at: 0 1218 1912 1944
Size: 1218 694 32
Fragments arranged by size:
1218 694 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1456 1944
Size: 331 1125 488
Fragments arranged by size:
1125 488 331
BsaI GGTCTCn'nnnn_
Cuts at: 0 409 1562 1944
Size: 409 1153 382
Fragments arranged by size:
1153 409 382
BsaHI Gr'CG_yC
Cuts at: 0 29 398 1944
Size: 29 369 1546
Fragments arranged by size:
1546 369 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 80 110 661 691 724 754 1327 1357
Size: 80 30 551 30 33 30 573 30
Cuts at: 1357 1944
Size: 587
Fragments arranged by size:
587 573 551 80 33 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 328 490 666 732 1087 1944
Size: 328 162 176 66 355 857
Fragments arranged by size:
857 355 328 176 162 66
BseYI C'CCAG_C
Cuts at: 0 246 559 631 680 1173 1245 1281 1448
Size: 246 313 72 49 493 72 36 167
Cuts at: 1448 1495 1530 1721 1918 1944
Size: 47 35 191 197 26
Fragments arranged by size:
493 313 246 197 191 167 72 72
49 47 36 35 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 1816 1944
Size: 1252 564 128
Fragments arranged by size:
1252 564 128
BsiHKAI G_wGCw'C
Cuts at: 0 911 1944
Size: 911 1033
Bsp1286I G_dGCh'C
Cuts at: 0 46 224 269 551 742 794 911 1041
Size: 46 178 45 282 191 52 117 130
Cuts at: 1041 1180 1337 1433 1449 1754 1821 1874 1944
Size: 139 157 96 16 305 67 53 70
Fragments arranged by size:
305 282 191 178 157 139 130 117
96 70 67 53 52 46 45 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1771 1838 1944
Size: 1771 67 106
Fragments arranged by size:
1771 106 67
BsrDI GCAATG_nn'
Cuts at: 0 206 1103 1944
Size: 206 897 841
Fragments arranged by size:
897 841 206
BsrFI r'CCGG_y
Cuts at: 0 130 250 1944
Size: 130 120 1694
Fragments arranged by size:
1694 130 120
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1028 1944
Size: 1028 916
BstEII G'GTnAC_C
Cuts at: 0 1683 1825 1944
Size: 1683 142 119
Fragments arranged by size:
1683 142 119
BstXI CCAn_nnnn'nTGG
Cuts at: 0 54 571 1515 1932 1944
Size: 54 517 944 417 12
Fragments arranged by size:
944 517 417 54 12
BstYI r'GATC_y
Cuts at: 0 479 887 1098 1944
Size: 479 408 211 846
Fragments arranged by size:
846 479 408 211
BtgI C'CryG_G
Cuts at: 0 1883 1931 1944
Size: 1883 48 13
Fragments arranged by size:
1883 48 13
BtsI GCAGTG_nn'
Cuts at: 0 184 240 1264 1734 1779 1809 1944
Size: 184 56 1024 470 45 30 135
Fragments arranged by size:
1024 470 184 135 56 45 30
DraIII CAC_nnn'GTG
Cuts at: 0 1825 1944
Size: 1825 119
EaeI y'GGCC_r
Cuts at: 0 1049 1124 1517 1944
Size: 1049 75 393 427
Fragments arranged by size:
1049 427 393 75
EarI CTCTTCn'nnn_
Cuts at: 0 808 1084 1130 1944
Size: 808 276 46 814
Fragments arranged by size:
814 808 276 46
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 511 1187 1944
Size: 511 676 757
Fragments arranged by size:
757 676 511
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 120 152 255 484 640 1271 1888
Size: 76 44 32 103 229 156 631 617
Cuts at: 1888 1944
Size: 56
Fragments arranged by size:
631 617 229 156 103 76 56 44
32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 82 1944
Size: 82 1862
EcoO109I rG'GnC_Cy
Cuts at: 0 43 75 220 266 1038 1222 1291 1320
Size: 43 32 145 46 772 184 69 29
Cuts at: 1320 1751 1897 1944
Size: 431 146 47
Fragments arranged by size:
772 431 184 146 145 69 47 46
43 32 29
EcoRI G'AATT_C
Cuts at: 0 948 1944
Size: 948 996
FspI TGC'GCA
Cuts at: 0 1114 1944
Size: 1114 830
HaeII r_GCGC'y
Cuts at: 0 69 1944
Size: 69 1875
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 988 1020 1083 1115 1944
Size: 988 32 63 32 829
Fragments arranged by size:
988 829 63 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 182 1944
Size: 182 1762
MscI TGG'CCA
Cuts at: 0 1126 1944
Size: 1126 818
MslI CAynn'nnrTG
Cuts at: 0 841 1108 1944
Size: 841 267 836
Fragments arranged by size:
841 836 267
MspA1I CmG'CkG
Cuts at: 0 140 462 1249 1944
Size: 140 322 787 695
Fragments arranged by size:
787 695 322 140
NaeI GCC'GGC
Cuts at: 0 132 252 1944
Size: 132 120 1692
Fragments arranged by size:
1692 132 120
NdeI CA'TA_TG
Cuts at: 0 665 1944
Size: 665 1279
NgoMIV G'CCGG_C
Cuts at: 0 130 250 1944
Size: 130 120 1694
Fragments arranged by size:
1694 130 120
NspI r_CATG'y
Cuts at: 0 130 1944
Size: 130 1814
PasI CC'CwG_GG
Cuts at: 0 262 334 454 1204 1747 1944
Size: 262 72 120 750 543 197
Fragments arranged by size:
750 543 262 197 120 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 585 712 765 1944
Size: 585 127 53 1179
Fragments arranged by size:
1179 585 127 53
PfoI T'CCnGG_A
Cuts at: 0 1392 1944
Size: 1392 552
PpuMI rG'GwC_Cy
Cuts at: 0 75 1320 1944
Size: 75 1245 624
Fragments arranged by size:
1245 624 75
PspOMI G'GGCC_C
Cuts at: 0 42 220 265 1037 1445 1750 1944
Size: 42 178 45 772 408 305 194
Fragments arranged by size:
772 408 305 194 178 45 42
PstI C_TGCA'G
Cuts at: 0 1319 1896 1944
Size: 1319 577 48
Fragments arranged by size:
1319 577 48
PvuII CAG'CTG
Cuts at: 0 140 1249 1944
Size: 140 1109 695
Fragments arranged by size:
1109 695 140
SapI GCTCTTCn'nnn_
Cuts at: 0 1130 1944
Size: 1130 814
SbfI CC_TGCA'GG
Cuts at: 0 1319 1896 1944
Size: 1319 577 48
Fragments arranged by size:
1319 577 48
ScaI AGT'ACT
Cuts at: 0 700 1944
Size: 700 1244
SfcI C'TryA_G
Cuts at: 0 825 1315 1892 1944
Size: 825 490 577 52
Fragments arranged by size:
825 577 490 52
SmaI CCC'GGG
Cuts at: 0 1799 1944
Size: 1799 145
SmlI C'TyrA_G
Cuts at: 0 346 850 1435 1944
Size: 346 504 585 509
Fragments arranged by size:
585 509 504 346
SphI G_CATG'C
Cuts at: 0 130 1944
Size: 130 1814
StuI AGG'CCT
Cuts at: 0 469 508 535 1382 1944
Size: 469 39 27 847 562
Fragments arranged by size:
847 562 469 39 27
StyI C'CwwG_G
Cuts at: 0 69 1572 1944
Size: 69 1503 372
Fragments arranged by size:
1503 372 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1295 1509 1813 1944
Size: 1295 214 304 131
Fragments arranged by size:
1295 304 214 131
TatI w'GTAC_w
Cuts at: 0 668 698 1944
Size: 668 30 1246
Fragments arranged by size:
1246 668 30
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 80 112 952 984 1327 1359 1944
Size: 80 32 840 32 343 32 585
Fragments arranged by size:
840 585 343 80 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 732 1233 1944
Size: 732 501 711
Fragments arranged by size:
732 711 501
XmaI C'CCGG_G
Cuts at: 0 1797 1944
Size: 1797 147
ZraI GAC'GTC
Cuts at: 0 30 399 1944
Size: 30 369 1545
Fragments arranged by size:
1545 369 30
Enzymes that do cut:
AarI AatII AcuI AflII ApaI ApoI AvaI BanI
BanII BbsI BbvCI BciVI BglI BglII Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NaeI NdeI NgoMIV NspI
PasI PflMI PfoI PpuMI PspOMI PstI PvuII SapI
SbfI ScaI SfcI SmaI SmlI SphI StuI StyI
TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BclI BfrBI BlpI BmtI
BplI BsaAI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NarI NcoI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI