Restriction map for
pFN21AB6158
(Linear) (Six-base) MAPSORT of: KIBB6158.seq Check: 2956 from: 1 to: 1938
KIBB6158.orf
With 182 enzymes: SgfI *
July 3, 2012 16:14 ..
AatII G_ACGT'C
Cuts at: 0 139 1938
Size: 139 1799
Acc65I G'GTAC_C
Cuts at: 0 1668 1938
Size: 1668 270
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 616 814 1235 1938
Size: 616 198 421 703
Fragments arranged by size:
703 616 421 198
AleI CACnn'nnGTG
Cuts at: 0 1288 1938
Size: 1288 650
AlwNI CAG_nnn'CTG
Cuts at: 0 250 870 924 980 1177 1189 1213 1790
Size: 250 620 54 56 197 12 24 577
Cuts at: 1790 1938
Size: 148
Fragments arranged by size:
620 577 250 197 148 56 54 24
12
ApaI G_GGCC'C
Cuts at: 0 77 1572 1938
Size: 77 1495 366
Fragments arranged by size:
1495 366 77
ApaLI G'TGCA_C
Cuts at: 0 1510 1938
Size: 1510 428
ApoI r'AATT_y
Cuts at: 0 567 1938
Size: 567 1371
AvaI C'yCGr_G
Cuts at: 0 76 145 896 1565 1938
Size: 76 69 751 669 373
Fragments arranged by size:
751 669 373 76 69
BanI G'GyrC_C
Cuts at: 0 149 664 1668 1938
Size: 149 515 1004 270
Fragments arranged by size:
1004 515 270 149
BanII G_rGCy'C
Cuts at: 0 77 422 772 1572 1799 1938
Size: 77 345 350 800 227 139
Fragments arranged by size:
800 350 345 227 139 77
BbeI G_GCGC'C
Cuts at: 0 153 1938
Size: 153 1785
BbsI GAAGACnn'nnnn_
Cuts at: 0 658 1223 1938
Size: 658 565 715
Fragments arranged by size:
715 658 565
BbvCI CC'TCA_GC
Cuts at: 0 1580 1938
Size: 1580 358
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 78 112 1938
Size: 78 34 1826
Fragments arranged by size:
1826 78 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1600 1938
Size: 1600 338
BglI GCCn_nnn'nGGC
Cuts at: 0 861 1055 1363 1492 1938
Size: 861 194 308 129 446
Fragments arranged by size:
861 446 308 194 129
BglII A'GATC_T
Cuts at: 0 1641 1938
Size: 1641 297
Bme1580I G_kGCm'C
Cuts at: 0 77 1514 1565 1572 1938
Size: 77 1437 51 7 366
Fragments arranged by size:
1437 366 77 51 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 745 1643 1938
Size: 745 898 295
Fragments arranged by size:
898 745 295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 668 1151 1862 1938
Size: 133 535 483 711 76
Fragments arranged by size:
711 535 483 133 76
Bpu10I CC'TnA_GC
Cuts at: 0 107 1580 1938
Size: 107 1473 358
Fragments arranged by size:
1473 358 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 502 541 1938
Size: 502 39 1397
Fragments arranged by size:
1397 502 39
BsaI GGTCTCn'nnnn_
Cuts at: 0 338 893 1107 1938
Size: 338 555 214 831
Fragments arranged by size:
831 555 338 214
BsaHI Gr'CG_yC
Cuts at: 0 136 150 1938
Size: 136 14 1788
Fragments arranged by size:
1788 136 14
BsaWI w'CCGG_w
Cuts at: 0 636 1105 1938
Size: 636 469 833
Fragments arranged by size:
833 636 469
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1591 1621 1938
Size: 1591 30 317
Fragments arranged by size:
1591 317 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1708 1938
Size: 1708 230
BseYI C'CCAG_C
Cuts at: 0 1014 1182 1338 1367 1584 1790 1866 1932
Size: 1014 168 156 29 217 206 76 66
Cuts at: 1932 1938
Size: 6
Fragments arranged by size:
1014 217 206 168 156 76 66 29
6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1938
Size: 322 1616
BsiEI CG_ry'CG
Cuts at: 0 50 71 177 963 1938
Size: 50 21 106 786 975
Fragments arranged by size:
975 786 106 50 21
BsiHKAI G_wGCw'C
Cuts at: 0 422 1311 1463 1514 1938
Size: 422 889 152 51 424
Fragments arranged by size:
889 424 422 152 51
BsmBI CGTCTCn'nnnn_
Cuts at: 0 775 793 1938
Size: 775 18 1145
Fragments arranged by size:
1145 775 18
Bsp1286I G_dGCh'C
Cuts at: 0 77 422 772 1311 1463 1514 1565 1572
Size: 77 345 350 539 152 51 51 7
Cuts at: 1572 1799 1938
Size: 227 139
Fragments arranged by size:
539 350 345 227 152 139 77 51
51 7
BspEI T'CCGG_A
Cuts at: 0 636 1938
Size: 636 1302
BspHI T'CATG_A
Cuts at: 0 1628 1938
Size: 1628 310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 503 810 1680 1892 1938
Size: 356 147 307 870 212 46
Fragments arranged by size:
870 356 307 212 147 46
BsrBI CCG'CTC
Cuts at: 0 61 1118 1938
Size: 61 1057 820
Fragments arranged by size:
1057 820 61
BsrFI r'CCGG_y
Cuts at: 0 13 1938
Size: 13 1925
BsrGI T'GTAC_A
Cuts at: 0 749 1938
Size: 749 1189
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 1290 1938
Size: 678 612 648
Fragments arranged by size:
678 648 612
BstYI r'GATC_y
Cuts at: 0 317 503 920 1641 1938
Size: 317 186 417 721 297
Fragments arranged by size:
721 417 317 297 186
Bsu36I CC'TnA_GG
Cuts at: 0 716 1938
Size: 716 1222
BtgI C'CryG_G
Cuts at: 0 708 1230 1550 1938
Size: 708 522 320 388
Fragments arranged by size:
708 522 388 320
BtsI GCAGTG_nn'
Cuts at: 0 850 1254 1699 1938
Size: 850 404 445 239
Fragments arranged by size:
850 445 404 239
DraIII CAC_nnn'GTG
Cuts at: 0 366 1231 1938
Size: 366 865 707
Fragments arranged by size:
865 707 366
EaeI y'GGCC_r
Cuts at: 0 47 68 122 174 872 1140 1280 1493
Size: 47 21 54 52 698 268 140 213
Cuts at: 1493 1938
Size: 445
Fragments arranged by size:
698 445 268 213 140 54 52 47
21
EagI C'GGCC_G
Cuts at: 0 47 68 174 1938
Size: 47 21 106 1764
Fragments arranged by size:
1764 106 47 21
EarI CTCTTCn'nnn_
Cuts at: 0 1063 1468 1652 1938
Size: 1063 405 184 286
Fragments arranged by size:
1063 405 286 184
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 736 1667 1938
Size: 736 931 271
Fragments arranged by size:
931 736 271
EcoICRI GAG'CTC
Cuts at: 0 420 1938
Size: 420 1518
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 616 668 814 1151 1235 1862 1938
Size: 133 483 52 146 337 84 627 76
Fragments arranged by size:
627 483 337 146 133 84 76 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1630 1938
Size: 1630 308
EcoO109I rG'GnC_Cy
Cuts at: 0 552 1569 1938
Size: 552 1017 369
Fragments arranged by size:
1017 552 369
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 231 1068 1440 1938
Size: 231 837 372 498
Fragments arranged by size:
837 498 372 231
FspI TGC'GCA
Cuts at: 0 1129 1938
Size: 1129 809
FspAI rTGC'GCAy
Cuts at: 0 1129 1938
Size: 1129 809
HaeII r_GCGC'y
Cuts at: 0 153 260 1938
Size: 153 107 1678
Fragments arranged by size:
1678 153 107
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 715 747 892 924 956 988 1062 1087
Size: 715 32 145 32 32 32 74 25
Cuts at: 1087 1094 1119 1938
Size: 7 25 819
Fragments arranged by size:
819 715 145 74 32 32 32 32
25 25 7
HincII GTy'rAC
Cuts at: 0 1404 1938
Size: 1404 534
KasI G'GCGC_C
Cuts at: 0 149 1938
Size: 149 1789
KpnI G_GTAC'C
Cuts at: 0 1672 1938
Size: 1672 266
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 1938
Size: 452 1486
MscI TGG'CCA
Cuts at: 0 1142 1282 1938
Size: 1142 140 656
Fragments arranged by size:
1142 656 140
MslI CAynn'nnrTG
Cuts at: 0 1148 1288 1480 1938
Size: 1148 140 192 458
Fragments arranged by size:
1148 458 192 140
MspA1I CmG'CkG
Cuts at: 0 173 224 809 980 1342 1584 1866 1938
Size: 173 51 585 171 362 242 282 72
Fragments arranged by size:
585 362 282 242 173 171 72 51
NarI GG'CG_CC
Cuts at: 0 150 1938
Size: 150 1788
NdeI CA'TA_TG
Cuts at: 0 1481 1938
Size: 1481 457
NotI GC'GGCC_GC
Cuts at: 0 47 174 1938
Size: 47 127 1764
Fragments arranged by size:
1764 127 47
PfoI T'CCnGG_A
Cuts at: 0 1919 1938
Size: 1919 19
PpuMI rG'GwC_Cy
Cuts at: 0 552 1938
Size: 552 1386
PspOMI G'GGCC_C
Cuts at: 0 73 1568 1938
Size: 73 1495 370
Fragments arranged by size:
1495 370 73
PstI C_TGCA'G
Cuts at: 0 353 427 1577 1694 1938
Size: 353 74 1150 117 244
Fragments arranged by size:
1150 353 244 117 74
PvuI CG_AT'CG
Cuts at: 0 963 1938
Size: 963 975
PvuII CAG'CTG
Cuts at: 0 980 1584 1866 1938
Size: 980 604 282 72
Fragments arranged by size:
980 604 282 72
SacI G_AGCT'C
Cuts at: 0 422 1938
Size: 422 1516
SapI GCTCTTCn'nnn_
Cuts at: 0 1468 1938
Size: 1468 470
ScaI AGT'ACT
Cuts at: 0 523 1938
Size: 523 1415
SfcI C'TryA_G
Cuts at: 0 349 423 1573 1690 1938
Size: 349 74 1150 117 248
Fragments arranged by size:
1150 349 248 117 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1363 1492 1938
Size: 1363 129 446
Fragments arranged by size:
1363 446 129
SfoI GGC'GCC
Cuts at: 0 151 1938
Size: 151 1787
SmaI CCC'GGG
Cuts at: 0 78 147 1567 1938
Size: 78 69 1420 371
Fragments arranged by size:
1420 371 78 69
SmlI C'TyrA_G
Cuts at: 0 517 556 1938
Size: 517 39 1382
Fragments arranged by size:
1382 517 39
SrfI GCCC'GGGC
Cuts at: 0 78 1938
Size: 78 1860
StyI C'CwwG_G
Cuts at: 0 944 1938
Size: 944 994
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1543 1938
Size: 1543 395
TatI w'GTAC_w
Cuts at: 0 521 749 1837 1938
Size: 521 228 1088 101
Fragments arranged by size:
1088 521 228 101
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1449 1938
Size: 1449 489
XmaI C'CCGG_G
Cuts at: 0 76 145 1565 1938
Size: 76 69 1420 373
Fragments arranged by size:
1420 373 76 69
ZraI GAC'GTC
Cuts at: 0 137 1938
Size: 137 1801
Enzymes that do cut:
AatII Acc65I AcuI AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
FspAI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NarI NdeI NotI PfoI PpuMI PspOMI
PstI PvuI PvuII SacI SapI ScaI SfcI SfiI
SfoI SmaI SmlI SrfI StyI TaqII TatI XcmI
XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BclI BfrBI BlpI BmgBI BmtI BplI BsaAI BsaBI
BsiWI BsmI BsrDI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV
FseI HindIII HpaI MfeI MluI NaeI NcoI NgoMIV
NheI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI RsrII
SacII SalI SanDI SbfI SexAI SgfI SgrAI SnaBI
SpeI SphI SspI StuI SwaI TstI Tth111I XbaI
XhoI XmnI