Restriction map for
pFN21AB6150
(Linear) (Six-base) MAPSORT of: KIBB6150.seq Check: 1729 from: 1 to: 1920
KIBB6150.orf
With 182 enzymes: SgfI *
March 22, 2012 15:33 ..
AatII G_ACGT'C
Cuts at: 0 1808 1920
Size: 1808 112
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 659 875 1697 1920
Size: 521 138 216 822 223
Fragments arranged by size:
822 521 223 216 138
AflIII A'CryG_T
Cuts at: 0 198 1920
Size: 198 1722
AgeI A'CCGG_T
Cuts at: 0 115 1920
Size: 115 1805
ApaI G_GGCC'C
Cuts at: 0 1667 1920
Size: 1667 253
ApaLI G'TGCA_C
Cuts at: 0 1295 1920
Size: 1295 625
ApoI r'AATT_y
Cuts at: 0 310 1414 1522 1627 1920
Size: 310 1104 108 105 293
Fragments arranged by size:
1104 310 293 108 105
AvaI C'yCGr_G
Cuts at: 0 23 1920
Size: 23 1897
AvrII C'CTAG_G
Cuts at: 0 1611 1920
Size: 1611 309
BamHI G'GATC_C
Cuts at: 0 1302 1920
Size: 1302 618
BanII G_rGCy'C
Cuts at: 0 95 1025 1667 1722 1920
Size: 95 930 642 55 198
Fragments arranged by size:
930 642 198 95 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 1613 1920
Size: 1613 307
BbvCI CC'TCA_GC
Cuts at: 0 35 1920
Size: 35 1885
BciVI GTATCCnnnnn_n'
Cuts at: 0 141 1920
Size: 141 1779
BclI T'GATC_A
Cuts at: 0 606 1586 1920
Size: 606 980 334
Fragments arranged by size:
980 606 334
BfrBI ATG'CAT
Cuts at: 0 886 1089 1920
Size: 886 203 831
Fragments arranged by size:
886 831 203
BlpI GC'TnA_GC
Cuts at: 0 1019 1721 1920
Size: 1019 702 199
Fragments arranged by size:
1019 702 199
Bme1580I G_kGCm'C
Cuts at: 0 1299 1667 1920
Size: 1299 368 253
Fragments arranged by size:
1299 368 253
BmgBI CAC'GTC
Cuts at: 0 958 1920
Size: 958 962
BmrI ACTGGGnnnn_n'
Cuts at: 0 858 903 1920
Size: 858 45 1017
Fragments arranged by size:
1017 858 45
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1039 1920
Size: 1039 881
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 1552 1920
Size: 818 734 368
Fragments arranged by size:
818 734 368
Bpu10I CC'TnA_GC
Cuts at: 0 35 488 1920
Size: 35 453 1432
Fragments arranged by size:
1432 453 35
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 1727 1920
Size: 82 1645 193
Fragments arranged by size:
1645 193 82
BsaAI yAC'GTr
Cuts at: 0 1876 1920
Size: 1876 44
BsaBI GATnn'nnATC
Cuts at: 0 605 1591 1920
Size: 605 986 329
Fragments arranged by size:
986 605 329
BsaHI Gr'CG_yC
Cuts at: 0 1805 1920
Size: 1805 115
BsaWI w'CCGG_w
Cuts at: 0 115 1920
Size: 115 1805
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 1920
Size: 85 1835
BseYI C'CCAG_C
Cuts at: 0 424 1183 1920
Size: 424 759 737
Fragments arranged by size:
759 737 424
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1257 1920
Size: 1257 663
BsiHKAI G_wGCw'C
Cuts at: 0 95 113 1025 1299 1722 1920
Size: 95 18 912 274 423 198
Fragments arranged by size:
912 423 274 198 95 18
BsmI GAATG_Cn'
Cuts at: 0 345 888 1920
Size: 345 543 1032
Fragments arranged by size:
1032 543 345
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1483 1920
Size: 1483 437
Bsp1286I G_dGCh'C
Cuts at: 0 95 113 1025 1299 1667 1722 1920
Size: 95 18 912 274 368 55 198
Fragments arranged by size:
912 368 274 198 95 55 18
BspHI T'CATG_A
Cuts at: 0 287 1920
Size: 287 1633
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 1920
Size: 921 999
BsrDI GCAATG_nn'
Cuts at: 0 549 1058 1920
Size: 549 509 862
Fragments arranged by size:
862 549 509
BsrFI r'CCGG_y
Cuts at: 0 115 1544 1920
Size: 115 1429 376
Fragments arranged by size:
1429 376 115
BsrGI T'GTAC_A
Cuts at: 0 517 1920
Size: 517 1403
BssSI C'ACGA_G
Cuts at: 0 106 1920
Size: 106 1814
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 223 1180 1920
Size: 86 137 957 740
Fragments arranged by size:
957 740 137 86
BstXI CCAn_nnnn'nTGG
Cuts at: 0 589 1920
Size: 589 1331
BstYI r'GATC_y
Cuts at: 0 1302 1920
Size: 1302 618
Bsu36I CC'TnA_GG
Cuts at: 0 1081 1920
Size: 1081 839
BtsI GCAGTG_nn'
Cuts at: 0 1713 1920
Size: 1713 207
DraI TTT'AAA
Cuts at: 0 777 1920
Size: 777 1143
EaeI y'GGCC_r
Cuts at: 0 44 1920
Size: 44 1876
EarI CTCTTCn'nnn_
Cuts at: 0 1105 1475 1811 1886 1920
Size: 1105 370 336 75 34
Fragments arranged by size:
1105 370 336 75 34
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 834 1920
Size: 834 1086
EcoICRI GAG'CTC
Cuts at: 0 93 1023 1720 1920
Size: 93 930 697 200
Fragments arranged by size:
930 697 200 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 659 818 875 1552 1697 1920
Size: 521 138 159 57 677 145 223
Fragments arranged by size:
677 521 223 159 145 138 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1615 1920
Size: 1615 305
EcoO109I rG'GnC_Cy
Cuts at: 0 1663 1920
Size: 1663 257
EcoRV GAT'ATC
Cuts at: 0 1125 1920
Size: 1125 795
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 1109 1114 1141 1146 1920
Size: 1030 32 47 5 27 5 774
Fragments arranged by size:
1030 774 47 32 27 5 5
HincII GTy'rAC
Cuts at: 0 800 1920
Size: 800 1120
HindIII A'AGCT_T
Cuts at: 0 769 1279 1920
Size: 769 510 641
Fragments arranged by size:
769 641 510
MfeI C'AATT_G
Cuts at: 0 1317 1920
Size: 1317 603
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 342 824 1920
Size: 183 159 482 1096
Fragments arranged by size:
1096 482 183 159
MspA1I CmG'CkG
Cuts at: 0 394 424 1920
Size: 394 30 1496
Fragments arranged by size:
1496 394 30
NaeI GCC'GGC
Cuts at: 0 1546 1920
Size: 1546 374
NdeI CA'TA_TG
Cuts at: 0 1027 1920
Size: 1027 893
NgoMIV G'CCGG_C
Cuts at: 0 1544 1920
Size: 1544 376
NsiI A_TGCA'T
Cuts at: 0 888 1091 1920
Size: 888 203 829
Fragments arranged by size:
888 829 203
NspI r_CATG'y
Cuts at: 0 202 1920
Size: 202 1718
PciI A'CATG_T
Cuts at: 0 198 1920
Size: 198 1722
PfoI T'CCnGG_A
Cuts at: 0 1568 1920
Size: 1568 352
PspOMI G'GGCC_C
Cuts at: 0 1663 1920
Size: 1663 257
PstI C_TGCA'G
Cuts at: 0 245 1920
Size: 245 1675
PvuII CAG'CTG
Cuts at: 0 394 424 1920
Size: 394 30 1496
Fragments arranged by size:
1496 394 30
SacI G_AGCT'C
Cuts at: 0 95 1025 1722 1920
Size: 95 930 697 198
Fragments arranged by size:
930 697 198 95
SapI GCTCTTCn'nnn_
Cuts at: 0 1886 1920
Size: 1886 34
ScaI AGT'ACT
Cuts at: 0 571 1920
Size: 571 1349
SfcI C'TryA_G
Cuts at: 0 241 1920
Size: 241 1679
SmaI CCC'GGG
Cuts at: 0 25 1920
Size: 25 1895
SmlI C'TyrA_G
Cuts at: 0 97 1742 1920
Size: 97 1645 178
Fragments arranged by size:
1645 178 97
StuI AGG'CCT
Cuts at: 0 917 1920
Size: 917 1003
StyI C'CwwG_G
Cuts at: 0 227 1611 1920
Size: 227 1384 309
Fragments arranged by size:
1384 309 227
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 481 1920
Size: 481 1439
TatI w'GTAC_w
Cuts at: 0 237 517 569 858 1920
Size: 237 280 52 289 1062
Fragments arranged by size:
1062 289 280 237 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1421 1453 1920
Size: 1421 32 467
Fragments arranged by size:
1421 467 32
XbaI T'CTAG_A
Cuts at: 0 936 1920
Size: 936 984
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1447 1920
Size: 1447 473
XmaI C'CCGG_G
Cuts at: 0 23 1920
Size: 23 1897
ZraI GAC'GTC
Cuts at: 0 1806 1920
Size: 1806 114
Enzymes that do cut:
AatII AcuI AflIII AgeI ApaI ApaLI ApoI AvaI
AvrII BamHI BanII BbsI BbvCI BciVI BclI BfrBI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BssSI BstAPI BstXI BstYI Bsu36I BtsI DraI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV Hin4I
HincII HindIII MfeI MmeI MspA1I NaeI NdeI NgoMIV
NsiI NspI PciI PfoI PspOMI PstI PvuII SacI
SapI ScaI SfcI SmaI SmlI StuI StyI TaqII
TatI TstI XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AhdI AleI
AlfI AloI AlwNI AscI AseI AsiSI BaeI BanI
BbeI BcgI BglI BglII BmtI BsaI BsaXI BsiEI
BsiWI BspEI BsrBI BssHII BstBI BstEII BstZ17I BtgI
BtgZI ClaI CspCI DraIII DrdI EagI EcoRI FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MscI MslI NarI NcoI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XhoI XmnI