Restriction map for
pFN21AB6149
(Linear) (Six-base) MAPSORT of: KIBB6149.seq Check: 764 from: 1 to: 1788
KIBB6149.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AccI GT'mk_AC
Cuts at: 0 185 536 1070 1788
Size: 185 351 534 718
Fragments arranged by size:
718 534 351 185
AclI AA'CG_TT
Cuts at: 0 848 1788
Size: 848 940
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 317 1371 1379 1676 1788
Size: 317 1054 8 297 112
Fragments arranged by size:
1054 317 297 112 8
AflII C'TTAA_G
Cuts at: 0 1659 1788
Size: 1659 129
AlwNI CAG_nnn'CTG
Cuts at: 0 700 1788
Size: 700 1088
ApaI G_GGCC'C
Cuts at: 0 21 1788
Size: 21 1767
ApoI r'AATT_y
Cuts at: 0 489 1236 1750 1788
Size: 489 747 514 38
Fragments arranged by size:
747 514 489 38
AseI AT'TA_AT
Cuts at: 0 811 1788
Size: 811 977
BanII G_rGCy'C
Cuts at: 0 21 83 1788
Size: 21 62 1705
Fragments arranged by size:
1705 62 21
BclI T'GATC_A
Cuts at: 0 906 1788
Size: 906 882
Bme1580I G_kGCm'C
Cuts at: 0 21 231 1788
Size: 21 210 1557
Fragments arranged by size:
1557 210 21
BmrI ACTGGGnnnn_n'
Cuts at: 0 1598 1788
Size: 1598 190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 1432 1685 1788
Size: 253 1179 253 103
Fragments arranged by size:
1179 253 253 103
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 1788
Size: 1322 466
BsaWI w'CCGG_w
Cuts at: 0 950 1788
Size: 950 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 251 281 1788
Size: 251 30 1507
Fragments arranged by size:
1507 251 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1391 1788
Size: 1391 397
BseYI C'CCAG_C
Cuts at: 0 114 1788
Size: 114 1674
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1788
Size: 826 962
BsiHKAI G_wGCw'C
Cuts at: 0 83 1788
Size: 83 1705
BsmI GAATG_Cn'
Cuts at: 0 1528 1707 1788
Size: 1528 179 81
Fragments arranged by size:
1528 179 81
Bsp1286I G_dGCh'C
Cuts at: 0 21 83 231 1788
Size: 21 62 148 1557
Fragments arranged by size:
1557 148 62 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 848 1788
Size: 65 783 940
Fragments arranged by size:
940 783 65
BsrFI r'CCGG_y
Cuts at: 0 1268 1788
Size: 1268 520
BssHII G'CGCG_C
Cuts at: 0 26 110 1788
Size: 26 84 1678
Fragments arranged by size:
1678 84 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 1788
Size: 45 1743
BstBI TT'CG_AA
Cuts at: 0 798 1788
Size: 798 990
BstEII G'GTnAC_C
Cuts at: 0 250 1788
Size: 250 1538
BstXI CCAn_nnnn'nTGG
Cuts at: 0 788 1788
Size: 788 1000
BstYI r'GATC_y
Cuts at: 0 1152 1362 1683 1788
Size: 1152 210 321 105
Fragments arranged by size:
1152 321 210 105
BstZ17I GTA'TAC
Cuts at: 0 537 1788
Size: 537 1251
Bsu36I CC'TnA_GG
Cuts at: 0 1486 1788
Size: 1486 302
BtgI C'CryG_G
Cuts at: 0 21 398 938 1788
Size: 21 377 540 850
Fragments arranged by size:
850 540 377 21
BtsI GCAGTG_nn'
Cuts at: 0 326 607 766 1788
Size: 326 281 159 1022
Fragments arranged by size:
1022 326 281 159
DraI TTT'AAA
Cuts at: 0 582 1319 1788
Size: 582 737 469
Fragments arranged by size:
737 582 469
EarI CTCTTCn'nnn_
Cuts at: 0 334 613 1022 1171 1258 1788
Size: 334 279 409 149 87 530
Fragments arranged by size:
530 409 334 279 149 87
EcoICRI GAG'CTC
Cuts at: 0 81 1788
Size: 81 1707
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 317 1371 1379 1432 1676 1685 1788
Size: 253 64 1054 8 53 244 9 103
Fragments arranged by size:
1054 253 244 103 64 53 9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 1788
Size: 259 1529
EcoO109I rG'GnC_Cy
Cuts at: 0 17 264 1664 1788
Size: 17 247 1400 124
Fragments arranged by size:
1400 247 124 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 267 299 1788
Size: 267 32 1489
Fragments arranged by size:
1489 267 32
HindIII A'AGCT_T
Cuts at: 0 1589 1788
Size: 1589 199
MslI CAynn'nnrTG
Cuts at: 0 985 1788
Size: 985 803
MspA1I CmG'CkG
Cuts at: 0 23 101 152 1146 1739 1788
Size: 23 78 51 994 593 49
Fragments arranged by size:
994 593 78 51 49 23
NcoI C'CATG_G
Cuts at: 0 938 1788
Size: 938 850
NdeI CA'TA_TG
Cuts at: 0 564 1788
Size: 564 1224
NspI r_CATG'y
Cuts at: 0 1023 1602 1788
Size: 1023 579 186
Fragments arranged by size:
1023 579 186
PflMI CCAn_nnn'nTGG
Cuts at: 0 276 1788
Size: 276 1512
PfoI T'CCnGG_A
Cuts at: 0 269 1409 1788
Size: 269 1140 379
Fragments arranged by size:
1140 379 269
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1725 1757 1788
Size: 1725 32 31
Fragments arranged by size:
1725 32 31
PpuMI rG'GwC_Cy
Cuts at: 0 264 1788
Size: 264 1524
PspOMI G'GGCC_C
Cuts at: 0 17 1788
Size: 17 1771
PvuII CAG'CTG
Cuts at: 0 101 152 1146 1739 1788
Size: 101 51 994 593 49
Fragments arranged by size:
994 593 101 51 49
SacI G_AGCT'C
Cuts at: 0 83 1788
Size: 83 1705
SacII CC_GC'GG
Cuts at: 0 24 1788
Size: 24 1764
SapI GCTCTTCn'nnn_
Cuts at: 0 1258 1788
Size: 1258 530
ScaI AGT'ACT
Cuts at: 0 440 1335 1788
Size: 440 895 453
Fragments arranged by size:
895 453 440
SfcI C'TryA_G
Cuts at: 0 1071 1788
Size: 1071 717
SmlI C'TyrA_G
Cuts at: 0 1337 1659 1788
Size: 1337 322 129
Fragments arranged by size:
1337 322 129
StuI AGG'CCT
Cuts at: 0 1184 1484 1788
Size: 1184 300 304
Fragments arranged by size:
1184 304 300
StyI C'CwwG_G
Cuts at: 0 189 787 938 1788
Size: 189 598 151 850
Fragments arranged by size:
850 598 189 151
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 677 852 1788
Size: 677 175 936
Fragments arranged by size:
936 677 175
TatI w'GTAC_w
Cuts at: 0 438 1031 1333 1775 1788
Size: 438 593 302 442 13
Fragments arranged by size:
593 442 438 302 13
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 937 969 1788
Size: 937 32 819
Fragments arranged by size:
937 819 32
Tth111I GACn'n_nGTC
Cuts at: 0 284 1788
Size: 284 1504
XbaI T'CTAG_A
Cuts at: 0 1206 1788
Size: 1206 582
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 196 396 1788
Size: 196 200 1392
Fragments arranged by size:
1392 200 196
Enzymes that do cut:
AccI AclI AcuI AflII AlwNI ApaI ApoI AseI
BanII BclI Bme1580I BmrI BpmI BpuEI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrFI
BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtsI DraI EarI EcoICRI Eco57MI EcoNI EcoO109I
Hin4I HindIII MslI MspA1I NcoI NdeI NspI PflMI
PfoI PpiI PpuMI PspOMI PvuII SacI SacII SapI
ScaI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BplI Bpu10I BsaI
BsaAI BsaBI BsaHI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssSI BtgZI ClaI CspCI DraIII
DrdI EaeI EagI EciI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XhoI XmaI XmnI
ZraI