(Linear) (Six-base) MAPSORT of: KIBB6106.seq  Check: 7461  from: 1  to: 1803



KIBB6106.orf

 With 182 enzymes: SgfI * 

                        July 3, 2012 16:13  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503    1803 
   Size:        503    1300
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1590    1803 
   Size:       1590     213
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     832    1803 
   Size:        832     971
 
AlwNI CAG_nnn'CTG

Cuts at:      0     972    1356    1803 
   Size:        972     384     447

  Fragments arranged by size: 

                 972     447     384
 
ApoI r'AATT_y

Cuts at:      0     554     671    1803 
   Size:        554     117    1132

  Fragments arranged by size: 

                1132     554     117
 
AvaI C'yCGr_G

Cuts at:      0    1297    1561    1803 
   Size:       1297     264     242

  Fragments arranged by size: 

                1297     264     242
 
BamHI G'GATC_C

Cuts at:      0     615    1040    1803 
   Size:        615     425     763

  Fragments arranged by size: 

                 763     615     425
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      56    1589    1658    1694    1715    1803 
   Size:         56    1533      69      36      21      88

  Fragments arranged by size: 

                1533      88      69      56      36      21
 
BlpI GC'TnA_GC

Cuts at:      0     125    1360    1803 
   Size:        125    1235     443

  Fragments arranged by size: 

                1235     443     125
 
BmgBI CAC'GTC

Cuts at:      0    1099    1607    1803 
   Size:       1099     508     196

  Fragments arranged by size: 

                1099     508     196
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      30    1803 
   Size:         30    1773
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     832    1803 
   Size:        832     971
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     298    1803 
   Size:        298    1505
 
BsaBI GATnn'nnATC

Cuts at:      0    1394    1803 
   Size:       1394     409
 
BsaHI Gr'CG_yC

Cuts at:      0    1290    1803 
   Size:       1290     513
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     535    1803 
   Size:        535    1268
 
BseYI C'CCAG_C

Cuts at:      0    1346    1803 
   Size:       1346     457
 
BsmI GAATG_Cn'

Cuts at:      0     154     898    1803 
   Size:        154     744     905

  Fragments arranged by size: 

                 905     744     154
 
BspHI T'CATG_A

Cuts at:      0     717    1803 
   Size:        717    1086
 
BsrGI T'GTAC_A

Cuts at:      0    1454    1803 
   Size:       1454     349
 
BssSI C'ACGA_G

Cuts at:      0    1474    1803 
   Size:       1474     329
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1356    1803 
   Size:       1356     447
 
BstYI r'GATC_y

Cuts at:      0     615    1040    1803 
   Size:        615     425     763

  Fragments arranged by size: 

                 763     615     425
 
Bsu36I CC'TnA_GG

Cuts at:      0    1189    1803 
   Size:       1189     614
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     472    1803 
   Size:        472    1331
 
DraI TTT'AAA

Cuts at:      0     942    1110    1803 
   Size:        942     168     693

  Fragments arranged by size: 

                 942     693     168
 
DraIII CAC_nnn'GTG

Cuts at:      0     450    1803 
   Size:        450    1353
 
EaeI y'GGCC_r

Cuts at:      0    1220    1478    1520    1803 
   Size:       1220     258      42     283

  Fragments arranged by size: 

                1220     283     258      42
 
EarI CTCTTCn'nnn_

Cuts at:      0     479     512    1803 
   Size:        479      33    1291

  Fragments arranged by size: 

                1291     479      33
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     865    1803 
   Size:        865     938
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     503     832    1803 
   Size:        503     329     971

  Fragments arranged by size: 

                 971     503     329
 
EcoO109I rG'GnC_Cy

Cuts at:      0     117    1803 
   Size:        117    1686
 
EcoRI G'AATT_C

Cuts at:      0     671    1803 
   Size:        671    1132
 
EcoRV GAT'ATC

Cuts at:      0     715    1803 
   Size:        715    1088
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1198    1732    1803 
   Size:       1198     534      71

  Fragments arranged by size: 

                1198     534      71
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     302     334    1280    1312    1496    1521    1528    1553 
   Size:        302      32     946      32     184      25       7      25

Cuts at:   1553    1576    1608    1747    1779    1803 
   Size:         23      32     139      32      24

  Fragments arranged by size: 

                 946     302     184     139      32      32      32      32
                  25      25      24      23       7
 
MfeI C'AATT_G

Cuts at:      0     200    1803 
   Size:        200    1603
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     281     758    1803 
   Size:        281     477    1045

  Fragments arranged by size: 

                1045     477     281
 
MspA1I CmG'CkG

Cuts at:      0     852     888    1518    1572    1803 
   Size:        852      36     630      54     231

  Fragments arranged by size: 

                 852     630     231      54      36
 
PpuMI rG'GwC_Cy

Cuts at:      0     117    1803 
   Size:        117    1686
 
PshAI GACnn'nnGTC

Cuts at:      0    1412    1803 
   Size:       1412     391
 
PvuII CAG'CTG

Cuts at:      0     852     888    1518    1803 
   Size:        852      36     630     285

  Fragments arranged by size: 

                 852     630     285      36
 
ScaI AGT'ACT

Cuts at:      0     133    1803 
   Size:        133    1670
 
SmlI C'TyrA_G

Cuts at:      0    1297    1803 
   Size:       1297     506
 
SspI AAT'ATT

Cuts at:      0     793    1803 
   Size:        793    1010
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1460    1803 
   Size:       1460     343
 
TatI w'GTAC_w

Cuts at:      0     131    1454    1803 
   Size:        131    1323     349

  Fragments arranged by size: 

                1323     349     131
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     847     879    1803 
   Size:        847      32     924

  Fragments arranged by size: 

                 924     847      32
 
XhoI C'TCGA_G

Cuts at:      0    1297    1803 
   Size:       1297     506
 
XmnI GAAnn'nnTTC

Cuts at:      0     502    1691    1803 
   Size:        502    1189     112

  Fragments arranged by size: 

                1189     502     112
 

 Enzymes that do cut:

     AcuI     AhdI     AlfI    AlwNI     ApoI     AvaI    BamHI     BbsI
     BlpI    BmgBI     BmrI     BpmI     BsaI    BsaBI    BsaHI    BseRI
    BseYI     BsmI    BspHI    BsrGI    BssSI   BstAPI    BstYI   Bsu36I
    BtgZI     DraI   DraIII     EaeI     EarI     EciI  Eco57MI EcoO109I
    EcoRI    EcoRV     FalI    Hin4I     MfeI     MmeI   MspA1I    PpuMI
    PshAI    PvuII     ScaI     SmlI     SspI    TaqII     TatI     TstI
     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII Bme1580I     BmtI     BplI   Bpu10I
    BpuEI    BsaAI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII
    BstBI   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI
     DrdI     EagI  EcoICRI    EcoNI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI  Tth111I     XbaI     XcmI
     XmaI     ZraI