Restriction map for
pFN21AB6015
(Linear) (Six-base) MAPSORT of: KIBB6015.seq Check: 5322 from: 1 to: 5484
KIBB6015.orf
With 182 enzymes: SgfI *
November 3, 2018 13:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1940 3498 3534 3735 4707 4968 5484
Size: 1940 1558 36 201 972 261 516
Fragments arranged by size:
1940 1558 972 516 261 201 36
AccI GT'mk_AC
Cuts at: 0 5275 5484
Size: 5275 209
Acc65I G'GTAC_C
Cuts at: 0 3441 5484
Size: 3441 2043
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1790 2515 2659 2670 3100 3121 3673
Size: 449 1341 725 144 11 430 21 552
Cuts at: 3673 4060 4423 4765 5484
Size: 387 363 342 719
Fragments arranged by size:
1341 725 719 552 449 430 387 363
342 144 21 11
AfeI AGC'GCT
Cuts at: 0 2749 5484
Size: 2749 2735
AflII C'TTAA_G
Cuts at: 0 3466 3804 5484
Size: 3466 338 1680
Fragments arranged by size:
3466 1680 338
AflIII A'CryG_T
Cuts at: 0 3452 5045 5484
Size: 3452 1593 439
Fragments arranged by size:
3452 1593 439
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1056 1238 5484
Size: 1056 182 4246
Fragments arranged by size:
4246 1056 182
AlwNI CAG_nnn'CTG
Cuts at: 0 427 2040 4038 4068 4071 4083 4722 5484
Size: 427 1613 1998 30 3 12 639 762
Fragments arranged by size:
1998 1613 762 639 427 30 12 3
ApaI G_GGCC'C
Cuts at: 0 1572 5484
Size: 1572 3912
ApaLI G'TGCA_C
Cuts at: 0 1604 3722 5484
Size: 1604 2118 1762
Fragments arranged by size:
2118 1762 1604
ApoI r'AATT_y
Cuts at: 0 817 971 1442 2113 5268 5484
Size: 817 154 471 671 3155 216
Fragments arranged by size:
3155 817 671 471 216 154
AseI AT'TA_AT
Cuts at: 0 371 1487 5484
Size: 371 1116 3997
Fragments arranged by size:
3997 1116 371
AvaI C'yCGr_G
Cuts at: 0 124 1025 5484
Size: 124 901 4459
Fragments arranged by size:
4459 901 124
BamHI G'GATC_C
Cuts at: 0 405 2315 5484
Size: 405 1910 3169
Fragments arranged by size:
3169 1910 405
BanI G'GyrC_C
Cuts at: 0 706 3441 4888 5484
Size: 706 2735 1447 596
Fragments arranged by size:
2735 1447 706 596
BanII G_rGCy'C
Cuts at: 0 17 1572 5484
Size: 17 1555 3912
Fragments arranged by size:
3912 1555 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 1398 2329 2902 3407 5484
Size: 1398 931 573 505 2077
Fragments arranged by size:
2077 1398 931 573 505
BbvCI CC'TCA_GC
Cuts at: 0 229 801 5484
Size: 229 572 4683
Fragments arranged by size:
4683 572 229
BciVI GTATCCnnnnn_n'
Cuts at: 0 35 5484
Size: 35 5449
BclI T'GATC_A
Cuts at: 0 4494 4970 5484
Size: 4494 476 514
Fragments arranged by size:
4494 514 476
BfrBI ATG'CAT
Cuts at: 0 376 1401 2658 3023 5484
Size: 376 1025 1257 365 2461
Fragments arranged by size:
2461 1257 1025 376 365
BglI GCCn_nnn'nGGC
Cuts at: 0 565 5484
Size: 565 4919
BglII A'GATC_T
Cuts at: 0 2240 4742 5484
Size: 2240 2502 742
Fragments arranged by size:
2502 2240 742
Bme1580I G_kGCm'C
Cuts at: 0 1572 1608 3702 3726 4891 5484
Size: 1572 36 2094 24 1165 593
Fragments arranged by size:
2094 1572 1165 593 36 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 2954 5484
Size: 2954 2530
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2754 5484
Size: 2754 2730
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3016 3457 3514 4854 5076 5264 5484
Size: 3016 441 57 1340 222 188 220
Fragments arranged by size:
3016 1340 441 222 220 188 57
Bpu10I CC'TnA_GC
Cuts at: 0 229 801 3146 3317 5484
Size: 229 572 2345 171 2167
Fragments arranged by size:
2345 2167 572 229 171
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3268 3601 5484
Size: 3268 333 1883
Fragments arranged by size:
3268 1883 333
BsaI GGTCTCn'nnnn_
Cuts at: 0 3815 5484
Size: 3815 1669
BsaAI yAC'GTr
Cuts at: 0 302 2397 4411 5484
Size: 302 2095 2014 1073
Fragments arranged by size:
2095 2014 1073 302
BsaBI GATnn'nnATC
Cuts at: 0 404 1020 5484
Size: 404 616 4464
Fragments arranged by size:
4464 616 404
BsaWI w'CCGG_w
Cuts at: 0 2312 2984 5484
Size: 2312 672 2500
Fragments arranged by size:
2500 2312 672
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 1035 1065 1827 1857 3160 3190
Size: 92 30 913 30 762 30 1303 30
Cuts at: 3190 5035 5065 5484
Size: 1845 30 419
Fragments arranged by size:
1845 1303 913 762 419 92 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 778 1120 1806 2664 3553 3784 4005 4060
Size: 778 342 686 858 889 231 221 55
Cuts at: 4060 5484
Size: 1424
Fragments arranged by size:
1424 889 858 778 686 342 231 221
55
BseYI C'CCAG_C
Cuts at: 0 3971 5363 5484
Size: 3971 1392 121
Fragments arranged by size:
3971 1392 121
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 1026 1912 2053 2746 3157 3614 5484
Size: 918 108 886 141 693 411 457 1870
Fragments arranged by size:
1870 918 886 693 457 411 141 108
BsiEI CG_ry'CG
Cuts at: 0 2084 5484
Size: 2084 3400
BsiHKAI G_wGCw'C
Cuts at: 0 17 257 1608 1892 2591 3551 3726 5053
Size: 17 240 1351 284 699 960 175 1327
Cuts at: 5053 5484
Size: 431
Fragments arranged by size:
1351 1327 960 699 431 284 240 175
17
BsmI GAATG_Cn'
Cuts at: 0 1197 1511 1540 5484
Size: 1197 314 29 3944
Fragments arranged by size:
3944 1197 314 29
BsmBI CGTCTCn'nnnn_
Cuts at: 0 4584 5484
Size: 4584 900
Bsp1286I G_dGCh'C
Cuts at: 0 17 257 1572 1608 1892 2591 3551 3702
Size: 17 240 1315 36 284 699 960 151
Cuts at: 3702 3726 4891 5053 5484
Size: 24 1165 162 431
Fragments arranged by size:
1315 1165 960 699 431 284 240 162
151 36 24 17
BspEI T'CCGG_A
Cuts at: 0 2312 2984 5484
Size: 2312 672 2500
Fragments arranged by size:
2500 2312 672
BspHI T'CATG_A
Cuts at: 0 240 5484
Size: 240 5244
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1940 3498 3534 3735 4091 4707 4968 5484
Size: 1940 1558 36 201 356 616 261 516
Fragments arranged by size:
1940 1558 616 516 356 261 201 36
BsrDI GCAATG_nn'
Cuts at: 0 1014 5238 5484
Size: 1014 4224 246
Fragments arranged by size:
4224 1014 246
BsrFI r'CCGG_y
Cuts at: 0 2083 2254 4443 5484
Size: 2083 171 2189 1041
Fragments arranged by size:
2189 2083 1041 171
BsrGI T'GTAC_A
Cuts at: 0 1580 3894 5484
Size: 1580 2314 1590
Fragments arranged by size:
2314 1590 1580
BssSI C'ACGA_G
Cuts at: 0 4126 4756 5484
Size: 4126 630 728
Fragments arranged by size:
4126 728 630
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3513 4068 4758 5484
Size: 3513 555 690 726
Fragments arranged by size:
3513 726 690 555
BstEII G'GTnAC_C
Cuts at: 0 3708 5484
Size: 3708 1776
BstYI r'GATC_y
Cuts at: 0 156 405 2240 2315 4028 4742 5484
Size: 156 249 1835 75 1713 714 742
Fragments arranged by size:
1835 1713 742 714 249 156 75
BstZ17I GTA'TAC
Cuts at: 0 5276 5484
Size: 5276 208
Bsu36I CC'TnA_GG
Cuts at: 0 1818 5484
Size: 1818 3666
BtgI C'CryG_G
Cuts at: 0 1447 4020 5484
Size: 1447 2573 1464
Fragments arranged by size:
2573 1464 1447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1214 5484
Size: 1214 4270
BtsI GCAGTG_nn'
Cuts at: 0 2036 4614 4680 5484
Size: 2036 2578 66 804
Fragments arranged by size:
2578 2036 804 66
ClaI AT'CG_AT
Cuts at: 0 3042 5484
Size: 3042 2442
DraI TTT'AAA
Cuts at: 0 822 5484
Size: 822 4662
DraIII CAC_nnn'GTG
Cuts at: 0 1971 4414 5484
Size: 1971 2443 1070
Fragments arranged by size:
2443 1971 1070
EaeI y'GGCC_r
Cuts at: 0 449 1208 1880 2081 2555 2699 4733 5484
Size: 449 759 672 201 474 144 2034 751
Fragments arranged by size:
2034 759 751 672 474 449 201 144
EagI C'GGCC_G
Cuts at: 0 2081 5484
Size: 2081 3403
EarI CTCTTCn'nnn_
Cuts at: 0 755 2017 2918 3284 3982 4634 5484
Size: 755 1262 901 366 698 652 850
Fragments arranged by size:
1262 901 850 755 698 652 366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2706 5484
Size: 2706 2778
EcoICRI GAG'CTC
Cuts at: 0 15 5484
Size: 15 5469
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1790 2515 2659 2670 3016 3100 3121
Size: 449 1341 725 144 11 346 84 21
Cuts at: 3121 3457 3514 3673 4060 4423 4765 4854 5076
Size: 336 57 159 387 363 342 89 222
Cuts at: 5076 5264 5484
Size: 188 220
Fragments arranged by size:
1341 725 449 387 363 346 342 336
222 220 188 159 144 89 84 57
21 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4042 5038 5484
Size: 4042 996 446
Fragments arranged by size:
4042 996 446
EcoO109I rG'GnC_Cy
Cuts at: 0 2952 3681 4014 5484
Size: 2952 729 333 1470
Fragments arranged by size:
2952 1470 729 333
EcoRV GAT'ATC
Cuts at: 0 694 3232 5484
Size: 694 2538 2252
Fragments arranged by size:
2538 2252 694
FspI TGC'GCA
Cuts at: 0 2437 3676 5484
Size: 2437 1239 1808
Fragments arranged by size:
2437 1808 1239
HaeII r_GCGC'y
Cuts at: 0 2751 5484
Size: 2751 2733
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 92 124 683 708 715 740
Size: 33 32 27 32 559 25 7 25
Cuts at: 740 759 791 3394 3426 4255 4287 5035 5067
Size: 19 32 2603 32 829 32 748 32
Cuts at: 5067 5484
Size: 417
Fragments arranged by size:
2603 829 748 559 417 33 32 32
32 32 32 32 27 25 25 19
7
HincII GTy'rAC
Cuts at: 0 4311 5092 5484
Size: 4311 781 392
Fragments arranged by size:
4311 781 392
HindIII A'AGCT_T
Cuts at: 0 434 1637 5484
Size: 434 1203 3847
Fragments arranged by size:
3847 1203 434
KpnI G_GTAC'C
Cuts at: 0 3445 5484
Size: 3445 2039
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 620 993 1674 1825 3864 4326 5484
Size: 620 373 681 151 2039 462 1158
Fragments arranged by size:
2039 1158 681 620 462 373 151
MscI TGG'CCA
Cuts at: 0 451 1210 1882 2557 2701 4735 5484
Size: 451 759 672 675 144 2034 749
Fragments arranged by size:
2034 759 749 675 672 451 144
MslI CAynn'nnrTG
Cuts at: 0 1093 1220 2383 2482 2770 4001 5484
Size: 1093 127 1163 99 288 1231 1483
Fragments arranged by size:
1483 1231 1163 1093 288 127 99
MspA1I CmG'CkG
Cuts at: 0 345 1040 2040 2793 3066 3753 3975 4068
Size: 345 695 1000 753 273 687 222 93
Cuts at: 4068 4089 5367 5484
Size: 21 1278 117
Fragments arranged by size:
1278 1000 753 695 687 345 273 222
117 93 21
NcoI C'CATG_G
Cuts at: 0 1447 5484
Size: 1447 4037
NsiI A_TGCA'T
Cuts at: 0 378 1403 2660 3025 5484
Size: 378 1025 1257 365 2459
Fragments arranged by size:
2459 1257 1025 378 365
NspI r_CATG'y
Cuts at: 0 1405 2051 2180 2367 2481 2658 2662 3037
Size: 1405 646 129 187 114 177 4 375
Cuts at: 3037 3313 3456 4477 4604 4806 5049 5070 5484
Size: 276 143 1021 127 202 243 21 414
Fragments arranged by size:
1405 1021 646 414 375 276 243 202
187 177 143 129 127 114 21 4
PciI A'CATG_T
Cuts at: 0 3452 5045 5484
Size: 3452 1593 439
Fragments arranged by size:
3452 1593 439
PflMI CCAn_nnn'nTGG
Cuts at: 0 38 5484
Size: 38 5446
PfoI T'CCnGG_A
Cuts at: 0 2057 3962 4434 5053 5484
Size: 2057 1905 472 619 431
Fragments arranged by size:
2057 1905 619 472 431
PmlI CAC'GTG
Cuts at: 0 4411 5484
Size: 4411 1073
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 4670 4702 5484
Size: 4670 32 782
Fragments arranged by size:
4670 782 32
PpuMI rG'GwC_Cy
Cuts at: 0 2952 5484
Size: 2952 2532
PsiI TTA'TAA
Cuts at: 0 92 5484
Size: 92 5392
PspOMI G'GGCC_C
Cuts at: 0 1568 5484
Size: 1568 3916
PspXI vC'TCGA_Gb
Cuts at: 0 124 5484
Size: 124 5360
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2809 2841 5484
Size: 2809 32 2643
Fragments arranged by size:
2809 2643 32
PstI C_TGCA'G
Cuts at: 0 1184 2798 2966 3980 4088 4124 5484
Size: 1184 1614 168 1014 108 36 1360
Fragments arranged by size:
1614 1360 1184 1014 168 108 36
PvuII CAG'CTG
Cuts at: 0 345 1040 2040 2793 3066 3753 3975 4068
Size: 345 695 1000 753 273 687 222 93
Cuts at: 4068 4089 5367 5484
Size: 21 1278 117
Fragments arranged by size:
1278 1000 753 695 687 345 273 222
117 93 21
RsrII CG'GwC_CG
Cuts at: 0 4194 5484
Size: 4194 1290
SacI G_AGCT'C
Cuts at: 0 17 5484
Size: 17 5467
ScaI AGT'ACT
Cuts at: 0 2470 5484
Size: 2470 3014
SfcI C'TryA_G
Cuts at: 0 1180 1381 2794 2962 3976 4084 4120 5484
Size: 1180 201 1413 168 1014 108 36 1364
Fragments arranged by size:
1413 1364 1180 1014 201 168 108 36
SmlI C'TyrA_G
Cuts at: 0 124 3283 3466 3616 3804 5484
Size: 124 3159 183 150 188 1680
Fragments arranged by size:
3159 1680 188 183 150 124
SnaBI TAC'GTA
Cuts at: 0 302 5484
Size: 302 5182
SphI G_CATG'C
Cuts at: 0 2367 2658 2662 4806 5070 5484
Size: 2367 291 4 2144 264 414
Fragments arranged by size:
2367 2144 414 291 264 4
StuI AGG'CCT
Cuts at: 0 931 5484
Size: 931 4553
StyI C'CwwG_G
Cuts at: 0 167 1447 2444 3563 3845 4338 5484
Size: 167 1280 997 1119 282 493 1146
Fragments arranged by size:
1280 1146 1119 997 493 282 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 385 1081 3361 4926 5484
Size: 385 696 2280 1565 558
Fragments arranged by size:
2280 1565 696 558 385
TatI w'GTAC_w
Cuts at: 0 1580 2468 3894 4628 5484
Size: 1580 888 1426 734 856
Fragments arranged by size:
1580 1426 888 856 734
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1035 1067 1663 1695 1827 1859 5484
Size: 1035 32 596 32 132 32 3625
Fragments arranged by size:
3625 1035 596 132 32 32 32
XbaI T'CTAG_A
Cuts at: 0 153 1515 2271 5484
Size: 153 1362 756 3213
Fragments arranged by size:
3213 1362 756 153
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 59 603 3882 4152 4698 5460 5484
Size: 59 544 3279 270 546 762 24
Fragments arranged by size:
3279 762 546 544 270 59 24
XhoI C'TCGA_G
Cuts at: 0 124 5484
Size: 124 5360
XmnI GAAnn'nnTTC
Cuts at: 0 1442 1688 1772 2909 3250 3404 4820 5484
Size: 1442 246 84 1137 341 154 1416 664
Fragments arranged by size:
1442 1416 1137 664 341 246 154 84
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AlfI
AlwNI ApaI ApaLI ApoI AseI AvaI BamHI BanI
BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI DraI DraIII EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I
NcoI NsiI NspI PciI PflMI PfoI PmlI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuII RsrII
SacI ScaI SfcI SmlI SnaBI SphI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AgeI AhdI AleI AloI AscI AsiSI
AvrII BaeI BbeI BcgI BlpI BmgBI BmtI BsaHI
BsiWI BsrBI BssHII BstBI BstXI CspCI DrdI EcoRI
FalI FseI FspAI HpaI KasI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PshAI PvuI SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SpeI SrfI
SspI SwaI Tth111I XmaI ZraI