Restriction map for
pFN21AB5989
(Linear) (Six-base) MAPSORT of: KIBB5989.seq Check: 5169 from: 1 to: 1719
KIBB5989.orf
With 182 enzymes: SgfI *
July 3, 2012 16:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 331 1719
Size: 331 1388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1641 1719
Size: 1641 78
AfeI AGC'GCT
Cuts at: 0 955 1719
Size: 955 764
AleI CACnn'nnGTG
Cuts at: 0 40 1719
Size: 40 1679
AlwNI CAG_nnn'CTG
Cuts at: 0 176 575 597 890 933 1429 1512 1719
Size: 176 399 22 293 43 496 83 207
Fragments arranged by size:
496 399 293 207 176 83 43 22
ApaI G_GGCC'C
Cuts at: 0 824 1719
Size: 824 895
AvaI C'yCGr_G
Cuts at: 0 287 1719
Size: 287 1432
BanI G'GyrC_C
Cuts at: 0 1144 1161 1719
Size: 1144 17 558
Fragments arranged by size:
1144 558 17
BanII G_rGCy'C
Cuts at: 0 146 294 385 824 856 1172 1448 1719
Size: 146 148 91 439 32 316 276 271
Fragments arranged by size:
439 316 276 271 148 146 91 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 1460 1598 1719
Size: 1460 138 121
Fragments arranged by size:
1460 138 121
BbvCI CC'TCA_GC
Cuts at: 0 1438 1637 1691 1719
Size: 1438 199 54 28
Fragments arranged by size:
1438 199 54 28
BglI GCCn_nnn'nGGC
Cuts at: 0 688 1321 1719
Size: 688 633 398
Fragments arranged by size:
688 633 398
BglII A'GATC_T
Cuts at: 0 996 1719
Size: 996 723
Bme1580I G_kGCm'C
Cuts at: 0 216 824 1719
Size: 216 608 895
Fragments arranged by size:
895 608 216
BmrI ACTGGGnnnn_n'
Cuts at: 0 124 870 900 1339 1719
Size: 124 746 30 439 380
Fragments arranged by size:
746 439 380 124 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 359 597 1719
Size: 110 249 238 1122
Fragments arranged by size:
1122 249 238 110
Bpu10I CC'TnA_GC
Cuts at: 0 602 1438 1637 1691 1719
Size: 602 836 199 54 28
Fragments arranged by size:
836 602 199 54 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1517 1719
Size: 1517 202
BsaI GGTCTCn'nnnn_
Cuts at: 0 1043 1719
Size: 1043 676
BsaAI yAC'GTr
Cuts at: 0 1567 1719
Size: 1567 152
BsaHI Gr'CG_yC
Cuts at: 0 1386 1719
Size: 1386 333
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1695 1719
Size: 1695 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 362 457 460 964 1395 1719
Size: 362 95 3 504 431 324
Fragments arranged by size:
504 431 362 324 95 3
BseYI C'CCAG_C
Cuts at: 0 298 453 483 1122 1719
Size: 298 155 30 639 597
Fragments arranged by size:
639 597 298 155 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1393 1719
Size: 1393 326
BsiHKAI G_wGCw'C
Cuts at: 0 146 1019 1073 1172 1448 1719
Size: 146 873 54 99 276 271
Fragments arranged by size:
873 276 271 146 99 54
Bsp1286I G_dGCh'C
Cuts at: 0 146 216 294 385 824 856 1019 1073
Size: 146 70 78 91 439 32 163 54
Cuts at: 1073 1172 1448 1719
Size: 99 276 271
Fragments arranged by size:
439 276 271 163 146 99 91 78
70 54 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 331 1719
Size: 331 1388
BsrDI GCAATG_nn'
Cuts at: 0 354 1719
Size: 354 1365
BsrGI T'GTAC_A
Cuts at: 0 1500 1719
Size: 1500 219
BstAPI GCAn_nnn'nTGC
Cuts at: 0 597 1719
Size: 597 1122
BstXI CCAn_nnnn'nTGG
Cuts at: 0 42 438 1406 1719
Size: 42 396 968 313
Fragments arranged by size:
968 396 313 42
BstYI r'GATC_y
Cuts at: 0 188 996 1719
Size: 188 808 723
Fragments arranged by size:
808 723 188
BtsI GCAGTG_nn'
Cuts at: 0 650 782 1376 1719
Size: 650 132 594 343
Fragments arranged by size:
650 594 343 132
DrdI GACnn_nn'nnGTC
Cuts at: 0 769 1719
Size: 769 950
EaeI y'GGCC_r
Cuts at: 0 804 966 1719
Size: 804 162 753
Fragments arranged by size:
804 753 162
EarI CTCTTCn'nnn_
Cuts at: 0 322 1719
Size: 322 1397
EcoICRI GAG'CTC
Cuts at: 0 144 1170 1446 1719
Size: 144 1026 276 273
Fragments arranged by size:
1026 276 273 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 359 597 1641 1719
Size: 110 249 238 1044 78
Fragments arranged by size:
1044 249 238 110 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 374 925 1719
Size: 374 551 794
Fragments arranged by size:
794 551 374
EcoO109I rG'GnC_Cy
Cuts at: 0 84 406 799 821 930 1672 1719
Size: 84 322 393 22 109 742 47
Fragments arranged by size:
742 393 322 109 84 47 22
HaeII r_GCGC'y
Cuts at: 0 957 1413 1512 1719
Size: 957 456 99 207
Fragments arranged by size:
957 456 207 99
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1092 1124 1216 1248 1371 1403 1719
Size: 1092 32 92 32 123 32 316
Fragments arranged by size:
1092 316 123 92 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1239 1332 1719
Size: 1239 93 387
Fragments arranged by size:
1239 387 93
MscI TGG'CCA
Cuts at: 0 968 1719
Size: 968 751
MslI CAynn'nnrTG
Cuts at: 0 40 436 1719
Size: 40 396 1283
Fragments arranged by size:
1283 396 40
MspA1I CmG'CkG
Cuts at: 0 40 161 176 663 1126 1268 1492 1641
Size: 40 121 15 487 463 142 224 149
Cuts at: 1641 1719
Size: 78
Fragments arranged by size:
487 463 224 149 142 121 78 40
15
PasI CC'CwG_GG
Cuts at: 0 385 402 927 1719
Size: 385 17 525 792
Fragments arranged by size:
792 525 385 17
PflMI CCAn_nnn'nTGG
Cuts at: 0 890 933 1719
Size: 890 43 786
Fragments arranged by size:
890 786 43
PfoI T'CCnGG_A
Cuts at: 0 626 1681 1719
Size: 626 1055 38
Fragments arranged by size:
1055 626 38
PmlI CAC'GTG
Cuts at: 0 1567 1719
Size: 1567 152
PpuMI rG'GwC_Cy
Cuts at: 0 406 799 1719
Size: 406 393 920
Fragments arranged by size:
920 406 393
PshAI GACnn'nnGTC
Cuts at: 0 281 1292 1719
Size: 281 1011 427
Fragments arranged by size:
1011 427 281
PspOMI G'GGCC_C
Cuts at: 0 820 1719
Size: 820 899
PstI C_TGCA'G
Cuts at: 0 593 1719
Size: 593 1126
PvuII CAG'CTG
Cuts at: 0 40 663 1126 1492 1719
Size: 40 623 463 366 227
Fragments arranged by size:
623 463 366 227 40
SacI G_AGCT'C
Cuts at: 0 146 1172 1448 1719
Size: 146 1026 276 271
Fragments arranged by size:
1026 276 271 146
SanDI GG'GwC_CC
Cuts at: 0 406 1719
Size: 406 1313
SexAI A'CCwGG_T
Cuts at: 0 978 1719
Size: 978 741
SfcI C'TryA_G
Cuts at: 0 168 508 522 589 754 1192 1629 1719
Size: 168 340 14 67 165 438 437 90
Fragments arranged by size:
438 437 340 168 165 90 67 14
SmlI C'TyrA_G
Cuts at: 0 1532 1719
Size: 1532 187
StuI AGG'CCT
Cuts at: 0 691 752 1284 1635 1719
Size: 691 61 532 351 84
Fragments arranged by size:
691 532 351 84 61
StyI C'CwwG_G
Cuts at: 0 553 848 1064 1364 1520 1719
Size: 553 295 216 300 156 199
Fragments arranged by size:
553 300 295 216 199 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1162 1719
Size: 1162 557
TatI w'GTAC_w
Cuts at: 0 878 1500 1597 1719
Size: 878 622 97 122
Fragments arranged by size:
878 622 122 97
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1289 1321 1719
Size: 1289 32 398
Fragments arranged by size:
1289 398 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 301 1050 1719
Size: 301 749 669
Fragments arranged by size:
749 669 301
Enzymes that do cut:
AarI AcuI AfeI AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BglI BglII Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspMI BsrDI BsrGI BstAPI BstXI
BstYI BtsI DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MmeI MscI MslI MspA1I PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SexAI SfcI SmlI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BlpI
BmgBI BmtI BplI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrFI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PsiI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI