Restriction map for
pFN21AB5958
(Linear) (Six-base) MAPSORT of: KIBB5958.seq Check: 5878 from: 1 to: 1650
KIBB5958.orf
With 182 enzymes: SgfI *
March 22, 2012 15:33 ..
AccI GT'mk_AC
Cuts at: 0 442 1650
Size: 442 1208
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 1637 1650
Size: 489 1148 13
Fragments arranged by size:
1148 489 13
AleI CACnn'nnGTG
Cuts at: 0 1360 1650
Size: 1360 290
AlwNI CAG_nnn'CTG
Cuts at: 0 1615 1650
Size: 1615 35
ApoI r'AATT_y
Cuts at: 0 415 660 984 1650
Size: 415 245 324 666
Fragments arranged by size:
666 415 324 245
AvaI C'yCGr_G
Cuts at: 0 1165 1650
Size: 1165 485
BamHI G'GATC_C
Cuts at: 0 758 1650
Size: 758 892
BanI G'GyrC_C
Cuts at: 0 88 1650
Size: 88 1562
BanII G_rGCy'C
Cuts at: 0 72 1166 1196 1650
Size: 72 1094 30 454
Fragments arranged by size:
1094 454 72 30
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 500 534 1650
Size: 500 34 1116
Fragments arranged by size:
1116 500 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1590 1650
Size: 1590 60
BmgBI CAC'GTC
Cuts at: 0 27 1650
Size: 27 1623
BmrI ACTGGGnnnn_n'
Cuts at: 0 1169 1650
Size: 1169 481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 649 953 1509 1650
Size: 151 498 304 556 141
Fragments arranged by size:
556 498 304 151 141
BsaI GGTCTCn'nnnn_
Cuts at: 0 778 1030 1528 1650
Size: 778 252 498 122
Fragments arranged by size:
778 498 252 122
BsaHI Gr'CG_yC
Cuts at: 0 16 1650
Size: 16 1634
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 616 646 1650
Size: 616 30 1004
Fragments arranged by size:
1004 616 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 1546 1650
Size: 118 1428 104
Fragments arranged by size:
1428 118 104
BseYI C'CCAG_C
Cuts at: 0 9 71 848 917 1650
Size: 9 62 777 69 733
Fragments arranged by size:
777 733 69 62 9
BsiHKAI G_wGCw'C
Cuts at: 0 1196 1355 1650
Size: 1196 159 295
Fragments arranged by size:
1196 295 159
Bsp1286I G_dGCh'C
Cuts at: 0 72 1166 1196 1355 1650
Size: 72 1094 30 159 295
Fragments arranged by size:
1094 295 159 72 30
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 788 1650
Size: 788 862
BsrDI GCAATG_nn'
Cuts at: 0 516 1254 1650
Size: 516 738 396
Fragments arranged by size:
738 516 396
BssSI C'ACGA_G
Cuts at: 0 890 1528 1650
Size: 890 638 122
Fragments arranged by size:
890 638 122
BstAPI GCAn_nnn'nTGC
Cuts at: 0 871 1650
Size: 871 779
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1596 1650
Size: 1596 54
BstYI r'GATC_y
Cuts at: 0 758 1175 1650
Size: 758 417 475
Fragments arranged by size:
758 475 417
BstZ17I GTA'TAC
Cuts at: 0 443 1650
Size: 443 1207
BtgI C'CryG_G
Cuts at: 0 1301 1650
Size: 1301 349
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 662 697 1650
Size: 662 35 953
Fragments arranged by size:
953 662 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 598 1650
Size: 598 1052
EaeI y'GGCC_r
Cuts at: 0 2 62 1082 1397 1650
Size: 2 60 1020 315 253
Fragments arranged by size:
1020 315 253 60 2
EarI CTCTTCn'nnn_
Cuts at: 0 1201 1650
Size: 1201 449
EcoICRI GAG'CTC
Cuts at: 0 1194 1650
Size: 1194 456
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 489 649 953 1509 1637 1650
Size: 151 338 160 304 556 128 13
Fragments arranged by size:
556 338 304 160 151 128 13
EcoRI G'AATT_C
Cuts at: 0 415 1650
Size: 415 1235
EcoRV GAT'ATC
Cuts at: 0 396 1650
Size: 396 1254
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 616 648 1269 1301 1650
Size: 616 32 621 32 349
Fragments arranged by size:
621 616 349 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 324 1650
Size: 324 1326
MscI TGG'CCA
Cuts at: 0 64 1084 1650
Size: 64 1020 566
Fragments arranged by size:
1020 566 64
MslI CAynn'nnrTG
Cuts at: 0 796 1323 1360 1408 1650
Size: 796 527 37 48 242
Fragments arranged by size:
796 527 242 48 37
MspA1I CmG'CkG
Cuts at: 0 53 75 1650
Size: 53 22 1575
Fragments arranged by size:
1575 53 22
NspI r_CATG'y
Cuts at: 0 543 1185 1650
Size: 543 642 465
Fragments arranged by size:
642 543 465
PflMI CCAn_nnn'nTGG
Cuts at: 0 1031 1650
Size: 1031 619
PfoI T'CCnGG_A
Cuts at: 0 167 1650
Size: 167 1483
PshAI GACnn'nnGTC
Cuts at: 0 1096 1650
Size: 1096 554
PstI C_TGCA'G
Cuts at: 0 389 1650
Size: 389 1261
PvuII CAG'CTG
Cuts at: 0 53 75 1650
Size: 53 22 1575
Fragments arranged by size:
1575 53 22
SacI G_AGCT'C
Cuts at: 0 1196 1650
Size: 1196 454
SapI GCTCTTCn'nnn_
Cuts at: 0 1201 1650
Size: 1201 449
SfcI C'TryA_G
Cuts at: 0 98 385 1650
Size: 98 287 1265
Fragments arranged by size:
1265 287 98
SphI G_CATG'C
Cuts at: 0 1185 1650
Size: 1185 465
StuI AGG'CCT
Cuts at: 0 1593 1650
Size: 1593 57
StyI C'CwwG_G
Cuts at: 0 473 1538 1626 1650
Size: 473 1065 88 24
Fragments arranged by size:
1065 473 88 24
TatI w'GTAC_w
Cuts at: 0 135 1650
Size: 135 1515
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1508 1540 1650
Size: 1508 32 110
Fragments arranged by size:
1508 110 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 72 228 1092 1407 1650
Size: 72 156 864 315 243
Fragments arranged by size:
864 315 243 156 72
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI AvaI BamHI BanI
BanII BcgI BglI BmgBI BmrI BpmI BsaI BsaHI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrDI BssSI
BstAPI BstXI BstYI BstZ17I BtgI CspCI DrdI EaeI
EarI EcoICRI Eco57MI EcoRI EcoRV Hin4I MmeI MscI
MslI MspA1I NspI PflMI PfoI PshAI PstI PvuII
SacI SapI SfcI SphI StuI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BbvCI BciVI BclI BfrBI
BglII BlpI Bme1580I BmtI BplI Bpu10I BpuEI BsaAI
BsaBI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrFI BsrGI BssHII BstBI BstEII Bsu36I
BtgZI BtsI ClaI DraI DraIII EagI EciI EcoNI
EcoO109I FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI