Restriction map for
pFN21AB5921
(Linear) (Six-base) MAPSORT of: KIBB5921.seq Check: 8754 from: 1 to: 1515
KIBB5921.orf
With 182 enzymes: SgfI *
March 22, 2012 15:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 312 1515
Size: 312 1203
Acc65I G'GTAC_C
Cuts at: 0 266 1203 1515
Size: 266 937 312
Fragments arranged by size:
937 312 266
AflIII A'CryG_T
Cuts at: 0 1472 1515
Size: 1472 43
AleI CACnn'nnGTG
Cuts at: 0 1270 1515
Size: 1270 245
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 1515
Size: 166 1349
ApaI G_GGCC'C
Cuts at: 0 46 1127 1515
Size: 46 1081 388
Fragments arranged by size:
1081 388 46
ApoI r'AATT_y
Cuts at: 0 481 1515
Size: 481 1034
AvaI C'yCGr_G
Cuts at: 0 45 375 782 871 1515
Size: 45 330 407 89 644
Fragments arranged by size:
644 407 330 89 45
BanI G'GyrC_C
Cuts at: 0 266 295 1203 1515
Size: 266 29 908 312
Fragments arranged by size:
908 312 266 29
BanII G_rGCy'C
Cuts at: 0 46 1127 1515
Size: 46 1081 388
Fragments arranged by size:
1081 388 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 1508 1515
Size: 1508 7
BbvCI CC'TCA_GC
Cuts at: 0 588 1515
Size: 588 927
BglI GCCn_nnn'nGGC
Cuts at: 0 763 1515
Size: 763 752
Bme1580I G_kGCm'C
Cuts at: 0 26 46 1127 1515
Size: 26 20 1081 388
Fragments arranged by size:
1081 388 26 20
BmgBI CAC'GTC
Cuts at: 0 339 1515
Size: 339 1176
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 297 603 1515
Size: 297 306 912
Fragments arranged by size:
912 306 297
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 721 1515
Size: 712 9 794
Fragments arranged by size:
794 712 9
Bpu10I CC'TnA_GC
Cuts at: 0 574 588 1515
Size: 574 14 927
Fragments arranged by size:
927 574 14
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 1420 1515
Size: 397 1023 95
Fragments arranged by size:
1023 397 95
BsaI GGTCTCn'nnnn_
Cuts at: 0 919 1515
Size: 919 596
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1219 1249 1515
Size: 1219 30 266
Fragments arranged by size:
1219 266 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 517 1515
Size: 514 3 998
Fragments arranged by size:
998 514 3
BseYI C'CCAG_C
Cuts at: 0 389 832 970 1069 1515
Size: 389 443 138 99 446
Fragments arranged by size:
446 443 389 138 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 282 1515
Size: 282 1233
BsiEI CG_ry'CG
Cuts at: 0 40 1515
Size: 40 1475
BsmBI CGTCTCn'nnnn_
Cuts at: 0 252 1515
Size: 252 1263
Bsp1286I G_dGCh'C
Cuts at: 0 26 46 1127 1515
Size: 26 20 1081 388
Fragments arranged by size:
1081 388 26 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 254 312 1515
Size: 254 58 1203
Fragments arranged by size:
1203 254 58
BsrDI GCAATG_nn'
Cuts at: 0 1161 1515
Size: 1161 354
BsrFI r'CCGG_y
Cuts at: 0 13 1515
Size: 13 1502
BsrGI T'GTAC_A
Cuts at: 0 1014 1515
Size: 1014 501
BstXI CCAn_nnnn'nTGG
Cuts at: 0 277 1515
Size: 277 1238
BstYI r'GATC_y
Cuts at: 0 254 966 1515
Size: 254 712 549
Fragments arranged by size:
712 549 254
Bsu36I CC'TnA_GG
Cuts at: 0 1169 1515
Size: 1169 346
BtgI C'CryG_G
Cuts at: 0 192 237 1194 1268 1515
Size: 192 45 957 74 247
Fragments arranged by size:
957 247 192 74 45
BtsI GCAGTG_nn'
Cuts at: 0 887 1494 1515
Size: 887 607 21
Fragments arranged by size:
887 607 21
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1179 1214 1470 1505 1515
Size: 1179 35 256 35 10
Fragments arranged by size:
1179 256 35 35 10
EaeI y'GGCC_r
Cuts at: 0 11 37 99 1277 1515
Size: 11 26 62 1178 238
Fragments arranged by size:
1178 238 62 26 11
EagI C'GGCC_G
Cuts at: 0 37 1515
Size: 37 1478
EarI CTCTTCn'nnn_
Cuts at: 0 491 1303 1490 1515
Size: 491 812 187 25
Fragments arranged by size:
812 491 187 25
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1515
Size: 154 1361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 712 721 1515
Size: 712 9 794
Fragments arranged by size:
794 712 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 100 1388 1515
Size: 100 1288 127
Fragments arranged by size:
1288 127 100
EcoO109I rG'GnC_Cy
Cuts at: 0 1123 1124 1515
Size: 1123 1 391
Fragments arranged by size:
1123 391 1
EcoRV GAT'ATC
Cuts at: 0 1446 1515
Size: 1446 69
HaeII r_GCGC'y
Cuts at: 0 20 1515
Size: 20 1495
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 595 607 627 639 776 808 1036 1068
Size: 595 12 20 12 137 32 228 32
Cuts at: 1068 1515
Size: 447
Fragments arranged by size:
595 447 228 137 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 114 1515
Size: 114 1401
KpnI G_GTAC'C
Cuts at: 0 270 1207 1515
Size: 270 937 308
Fragments arranged by size:
937 308 270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 902 1061 1154 1515
Size: 902 159 93 361
Fragments arranged by size:
902 361 159 93
MslI CAynn'nnrTG
Cuts at: 0 892 1270 1515
Size: 892 378 245
Fragments arranged by size:
892 378 245
MspA1I CmG'CkG
Cuts at: 0 78 150 225 389 690 918 1283 1515
Size: 78 72 75 164 301 228 365 232
Fragments arranged by size:
365 301 232 228 164 78 75 72
NaeI GCC'GGC
Cuts at: 0 15 1515
Size: 15 1500
NdeI CA'TA_TG
Cuts at: 0 1304 1515
Size: 1304 211
NgoMIV G'CCGG_C
Cuts at: 0 13 1515
Size: 13 1502
NspI r_CATG'y
Cuts at: 0 384 1515
Size: 384 1131
PspOMI G'GGCC_C
Cuts at: 0 42 1123 1515
Size: 42 1081 392
Fragments arranged by size:
1081 392 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 620 652 1515
Size: 620 32 863
Fragments arranged by size:
863 620 32
PstI C_TGCA'G
Cuts at: 0 1288 1515
Size: 1288 227
PvuII CAG'CTG
Cuts at: 0 225 389 690 918 1283 1515
Size: 225 164 301 228 365 232
Fragments arranged by size:
365 301 232 228 225 164
SapI GCTCTTCn'nnn_
Cuts at: 0 1303 1515
Size: 1303 212
SfcI C'TryA_G
Cuts at: 0 1284 1356 1371 1515
Size: 1284 72 15 144
Fragments arranged by size:
1284 144 72 15
SmaI CCC'GGG
Cuts at: 0 47 377 873 1515
Size: 47 330 496 642
Fragments arranged by size:
642 496 330 47
SmlI C'TyrA_G
Cuts at: 0 412 1435 1515
Size: 412 1023 80
Fragments arranged by size:
1023 412 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1365 1515
Size: 1365 150
TatI w'GTAC_w
Cuts at: 0 786 1014 1515
Size: 786 228 501
Fragments arranged by size:
786 501 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 933 965 1515
Size: 933 32 550
Fragments arranged by size:
933 550 32
Tth111I GACn'n_nGTC
Cuts at: 0 421 1384 1515
Size: 421 963 131
Fragments arranged by size:
963 421 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 846 1448 1515
Size: 846 602 67
Fragments arranged by size:
846 602 67
XmaI C'CCGG_G
Cuts at: 0 45 375 871 1515
Size: 45 330 496 644
Fragments arranged by size:
644 496 330 45
XmnI GAAnn'nnTTC
Cuts at: 0 1474 1515
Size: 1474 41
Enzymes that do cut:
AarI Acc65I AflIII AleI AlfI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BglI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI BsgI
BsiEI BsmBI Bsp1286I BspMI BsrDI BsrFI BsrGI BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII
KpnI MmeI MslI MspA1I NaeI NdeI NgoMIV NspI
PspOMI PsrI PstI PvuII SapI SfcI SmaI SmlI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AgeI AhdI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglII BlpI
BmrI BmtI BsaAI BsaBI BsaHI BsaWI BsiHKAI BsiWI
BsmI BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI DraI DraIII DrdI EcoICRI
EcoRI FalI FseI FspI FspAI HindIII HpaI KasI
MfeI MluI MscI NarI NcoI NheI NotI NruI
NsiI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI XbaI XhoI ZraI