Restriction map for
pFN21AB5917
(Linear) (Six-base) MAPSORT of: KIBB5917.seq Check: 330 from: 1 to: 1515
KIBB5917.orf
With 182 enzymes: SgfI *
July 15, 2010 18:30 ..
AatII G_ACGT'C
Cuts at: 0 515 1515
Size: 515 1000
AccI GT'mk_AC
Cuts at: 0 1231 1515
Size: 1231 284
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 609 1352 1433 1515
Size: 568 41 743 81 82
Fragments arranged by size:
743 568 82 81 41
AflIII A'CryG_T
Cuts at: 0 641 1114 1515
Size: 641 473 401
Fragments arranged by size:
641 473 401
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 49 1515
Size: 49 1466
AlwNI CAG_nnn'CTG
Cuts at: 0 843 1164 1515
Size: 843 321 351
Fragments arranged by size:
843 351 321
ApaI G_GGCC'C
Cuts at: 0 221 1515
Size: 221 1294
ApaLI G'TGCA_C
Cuts at: 0 675 1045 1273 1515
Size: 675 370 228 242
Fragments arranged by size:
675 370 242 228
AscI GG'CGCG_CC
Cuts at: 0 15 1515
Size: 15 1500
AvaI C'yCGr_G
Cuts at: 0 60 151 982 1322 1501 1515
Size: 60 91 831 340 179 14
Fragments arranged by size:
831 340 179 91 60 14
BanI G'GyrC_C
Cuts at: 0 196 211 254 1378 1515
Size: 196 15 43 1124 137
Fragments arranged by size:
1124 196 137 43 15
BanII G_rGCy'C
Cuts at: 0 61 130 158 182 221 698 1069 1091
Size: 61 69 28 24 39 477 371 22
Cuts at: 1091 1515
Size: 424
Fragments arranged by size:
477 424 371 69 61 39 28 24
22
BbeI G_GCGC'C
Cuts at: 0 200 215 258 1515
Size: 200 15 43 1257
Fragments arranged by size:
1257 200 43 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 428 475 1358 1452 1515
Size: 428 47 883 94 63
Fragments arranged by size:
883 428 94 63 47
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 647 681 1515
Size: 647 34 834
Fragments arranged by size:
834 647 34
BglII A'GATC_T
Cuts at: 0 347 710 1515
Size: 347 363 805
Fragments arranged by size:
805 363 347
BlpI GC'TnA_GC
Cuts at: 0 1307 1515
Size: 1307 208
Bme1580I G_kGCm'C
Cuts at: 0 221 679 925 1049 1076 1114 1277 1515
Size: 221 458 246 124 27 38 163 238
Fragments arranged by size:
458 246 238 221 163 124 38 27
BmgBI CAC'GTC
Cuts at: 0 114 1515
Size: 114 1401
BmrI ACTGGGnnnn_n'
Cuts at: 0 908 1515
Size: 908 607
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 732 1348 1515
Size: 732 616 167
Fragments arranged by size:
732 616 167
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 232 511 727 1515
Size: 58 174 279 216 788
Fragments arranged by size:
788 279 216 174 58
Bpu10I CC'TnA_GC
Cuts at: 0 1000 1515
Size: 1000 515
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 448 1515
Size: 448 1067
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1515
Size: 979 536
BsaAI yAC'GTr
Cuts at: 0 1244 1515
Size: 1244 271
BsaHI Gr'CG_yC
Cuts at: 0 4 197 212 255 281 512 814 1254
Size: 4 193 15 43 26 231 302 440
Cuts at: 1254 1515
Size: 261
Fragments arranged by size:
440 302 261 231 193 43 26 15
4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 484 514 685 715 1515
Size: 484 30 171 30 800
Fragments arranged by size:
800 484 171 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 955 1515
Size: 955 560
BseYI C'CCAG_C
Cuts at: 0 291 557 865 1515
Size: 291 266 308 650
Fragments arranged by size:
650 308 291 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 475 1224 1240 1337 1420 1515
Size: 475 749 16 97 83 95
Fragments arranged by size:
749 475 97 95 83 16
BsiEI CG_ry'CG
Cuts at: 0 315 554 1515
Size: 315 239 961
Fragments arranged by size:
961 315 239
BsiHKAI G_wGCw'C
Cuts at: 0 679 1049 1091 1277 1334 1515
Size: 679 370 42 186 57 181
Fragments arranged by size:
679 370 186 181 57 42
BsmBI CGTCTCn'nnnn_
Cuts at: 0 519 1515
Size: 519 996
Bsp1286I G_dGCh'C
Cuts at: 0 61 130 158 182 221 679 698 925
Size: 61 69 28 24 39 458 19 227
Cuts at: 925 1049 1069 1076 1091 1114 1277 1334 1515
Size: 124 20 7 15 23 163 57 181
Fragments arranged by size:
458 227 181 163 124 69 61 57
39 28 24 23 20 19 15 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 1442 1515
Size: 447 995 73
Fragments arranged by size:
995 447 73
BsrFI r'CCGG_y
Cuts at: 0 67 283 1004 1515
Size: 67 216 721 511
Fragments arranged by size:
721 511 216 67
BssHII G'CGCG_C
Cuts at: 0 15 45 1515
Size: 15 30 1470
Fragments arranged by size:
1470 30 15
BssSI C'ACGA_G
Cuts at: 0 1440 1515
Size: 1440 75
BstAPI GCAn_nnn'nTGC
Cuts at: 0 90 1515
Size: 90 1425
BstXI CCAn_nnnn'nTGG
Cuts at: 0 705 1041 1515
Size: 705 336 474
Fragments arranged by size:
705 474 336
BstYI r'GATC_y
Cuts at: 0 347 710 1421 1515
Size: 347 363 711 94
Fragments arranged by size:
711 363 347 94
Bsu36I CC'TnA_GG
Cuts at: 0 745 1515
Size: 745 770
BtgI C'CryG_G
Cuts at: 0 166 200 817 1515
Size: 166 34 617 698
Fragments arranged by size:
698 617 166 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 93 1014 1515
Size: 93 921 501
Fragments arranged by size:
921 501 93
DraIII CAC_nnn'GTG
Cuts at: 0 1125 1298 1515
Size: 1125 173 217
Fragments arranged by size:
1125 217 173
EaeI y'GGCC_r
Cuts at: 0 312 1006 1484 1515
Size: 312 694 478 31
Fragments arranged by size:
694 478 312 31
EagI C'GGCC_G
Cuts at: 0 312 1515
Size: 312 1203
EarI CTCTTCn'nnn_
Cuts at: 0 664 745 1515
Size: 664 81 770
Fragments arranged by size:
770 664 81
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 121 1515
Size: 121 1394
EcoICRI GAG'CTC
Cuts at: 0 1089 1515
Size: 1089 426
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 232 511 568 609 727 1352 1433
Size: 58 174 279 57 41 118 625 81
Cuts at: 1433 1515
Size: 82
Fragments arranged by size:
625 279 174 118 82 81 58 57
41
EcoO109I rG'GnC_Cy
Cuts at: 0 218 381 459 545 996 1054 1515
Size: 218 163 78 86 451 58 461
Fragments arranged by size:
461 451 218 163 86 78 58
FspI TGC'GCA
Cuts at: 0 271 1105 1515
Size: 271 834 410
Fragments arranged by size:
834 410 271
HaeII r_GCGC'y
Cuts at: 0 164 200 215 241 258 1515
Size: 164 36 15 26 17 1257
Fragments arranged by size:
1257 164 36 26 17 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 1423 1455 1515
Size: 415 32 976 32 60
Fragments arranged by size:
976 415 60 32 32
KasI G'GCGC_C
Cuts at: 0 196 211 254 1515
Size: 196 15 43 1261
Fragments arranged by size:
1261 196 43 15
MluI A'CGCG_T
Cuts at: 0 1114 1515
Size: 1114 401
MspA1I CmG'CkG
Cuts at: 0 87 168 202 228 252 367 588 819
Size: 87 81 34 26 24 115 221 231
Cuts at: 819 865 909 1428 1515
Size: 46 44 519 87
Fragments arranged by size:
519 231 221 115 87 87 81 46
44 34 26 24
NaeI GCC'GGC
Cuts at: 0 69 1006 1515
Size: 69 937 509
Fragments arranged by size:
937 509 69
NarI GG'CG_CC
Cuts at: 0 197 212 255 1515
Size: 197 15 43 1260
Fragments arranged by size:
1260 197 43 15
NdeI CA'TA_TG
Cuts at: 0 660 1515
Size: 660 855
NgoMIV G'CCGG_C
Cuts at: 0 67 1004 1515
Size: 67 937 511
Fragments arranged by size:
937 511 67
NspI r_CATG'y
Cuts at: 0 645 1515
Size: 645 870
PciI A'CATG_T
Cuts at: 0 641 1515
Size: 641 874
PflMI CCAn_nnn'nTGG
Cuts at: 0 1188 1515
Size: 1188 327
PfoI T'CCnGG_A
Cuts at: 0 1394 1515
Size: 1394 121
PmlI CAC'GTG
Cuts at: 0 1244 1515
Size: 1244 271
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 950 982 1515
Size: 950 32 533
Fragments arranged by size:
950 533 32
PpuMI rG'GwC_Cy
Cuts at: 0 459 545 1515
Size: 459 86 970
Fragments arranged by size:
970 459 86
PshAI GACnn'nnGTC
Cuts at: 0 146 550 1515
Size: 146 404 965
Fragments arranged by size:
965 404 146
PspOMI G'GGCC_C
Cuts at: 0 217 1515
Size: 217 1298
PstI C_TGCA'G
Cuts at: 0 839 1264 1361 1515
Size: 839 425 97 154
Fragments arranged by size:
839 425 154 97
PvuII CAG'CTG
Cuts at: 0 228 367 865 909 1428 1515
Size: 228 139 498 44 519 87
Fragments arranged by size:
519 498 228 139 87 44
SacI G_AGCT'C
Cuts at: 0 1091 1515
Size: 1091 424
SacII CC_GC'GG
Cuts at: 0 169 203 820 1515
Size: 169 34 617 695
Fragments arranged by size:
695 617 169 34
SfcI C'TryA_G
Cuts at: 0 835 1232 1260 1357 1515
Size: 835 397 28 97 158
Fragments arranged by size:
835 397 158 97 28
SfoI GGC'GCC
Cuts at: 0 198 213 256 1515
Size: 198 15 43 1259
Fragments arranged by size:
1259 198 43 15
SgrAI Cr'CCGG_yG
Cuts at: 0 283 1515
Size: 283 1232
SmlI C'TyrA_G
Cuts at: 0 463 1515
Size: 463 1052
StyI C'CwwG_G
Cuts at: 0 752 1061 1515
Size: 752 309 454
Fragments arranged by size:
752 454 309
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 540 1515
Size: 540 975
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1189 1515
Size: 1189 326
ZraI GAC'GTC
Cuts at: 0 513 1515
Size: 513 1002
Enzymes that do cut:
AatII AccI AcuI AflIII AlfI AlwNI ApaI ApaLI
AscI AvaI BanI BanII BbeI BbsI BcgI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI DraIII EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I FspI HaeII Hin4I
KasI MluI MspA1I NaeI NarI NdeI NgoMIV NspI
PciI PflMI PfoI PmlI PpiI PpuMI PshAI PspOMI
PstI PvuII SacI SacII SfcI SfoI SgrAI SmlI
StyI TaqII XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApoI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BglI BmtI BsaBI BsaWI BsiWI
BsmI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII
BstZ17I BtsI ClaI CspCI DraI DrdI EcoNI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI
MfeI MmeI MscI MslI NcoI NheI NotI NruI
NsiI PacI PasI PmeI PsiI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI