Restriction map for
pFN21AB5896
(Linear) (Six-base) MAPSORT of: KIBB5896.seq Check: 7744 from: 1 to: 1572
KIBB5896.orf
With 182 enzymes: SgfI *
December 3, 2014 09:29 ..
AccI GT'mk_AC
Cuts at: 0 1271 1572
Size: 1271 301
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1372 1572
Size: 1372 200
AflIII A'CryG_T
Cuts at: 0 627 1572
Size: 627 945
AhdI GACnn_n'nnGTC
Cuts at: 0 90 1278 1572
Size: 90 1188 294
Fragments arranged by size:
1188 294 90
AleI CACnn'nnGTG
Cuts at: 0 997 1572
Size: 997 575
ApoI r'AATT_y
Cuts at: 0 218 280 1572
Size: 218 62 1292
Fragments arranged by size:
1292 218 62
AseI AT'TA_AT
Cuts at: 0 839 1572
Size: 839 733
BanI G'GyrC_C
Cuts at: 0 1230 1572
Size: 1230 342
BanII G_rGCy'C
Cuts at: 0 309 1572
Size: 309 1263
BbsI GAAGACnn'nnnn_
Cuts at: 0 676 1572
Size: 676 896
BclI T'GATC_A
Cuts at: 0 869 1535 1572
Size: 869 666 37
Fragments arranged by size:
869 666 37
Bme1580I G_kGCm'C
Cuts at: 0 1173 1572
Size: 1173 399
BmrI ACTGGGnnnn_n'
Cuts at: 0 820 1572
Size: 820 752
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 443 1572
Size: 443 1129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 95 296 1572
Size: 17 78 201 1276
Fragments arranged by size:
1276 201 78 17
Bpu10I CC'TnA_GC
Cuts at: 0 1184 1572
Size: 1184 388
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1365 1572
Size: 1365 207
BsaI GGTCTCn'nnnn_
Cuts at: 0 1286 1572
Size: 1286 286
BsaWI w'CCGG_w
Cuts at: 0 558 1572
Size: 558 1014
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 522 552 1572
Size: 522 30 1020
Fragments arranged by size:
1020 522 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 215 419 523 526 527 1572
Size: 215 204 104 3 1 1045
Fragments arranged by size:
1045 215 204 104 3 1
BseYI C'CCAG_C
Cuts at: 0 979 1572
Size: 979 593
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1572
Size: 593 979
BsiEI CG_ry'CG
Cuts at: 0 559 1572
Size: 559 1013
BsiHKAI G_wGCw'C
Cuts at: 0 245 1572
Size: 245 1327
BsmI GAATG_Cn'
Cuts at: 0 477 1572
Size: 477 1095
Bsp1286I G_dGCh'C
Cuts at: 0 245 309 1173 1572
Size: 245 64 864 399
Fragments arranged by size:
864 399 245 64
BsrDI GCAATG_nn'
Cuts at: 0 1562 1572
Size: 1562 10
BstAPI GCAn_nnn'nTGC
Cuts at: 0 472 1033 1572
Size: 472 561 539
Fragments arranged by size:
561 539 472
BstEII G'GTnAC_C
Cuts at: 0 727 1572
Size: 727 845
BstXI CCAn_nnnn'nTGG
Cuts at: 0 302 1572
Size: 302 1270
BstYI r'GATC_y
Cuts at: 0 1138 1572
Size: 1138 434
Bsu36I CC'TnA_GG
Cuts at: 0 359 1572
Size: 359 1213
BtgI C'CryG_G
Cuts at: 0 1205 1572
Size: 1205 367
BtsI GCAGTG_nn'
Cuts at: 0 791 1572
Size: 791 781
ClaI AT'CG_AT
Cuts at: 0 1434 1572
Size: 1434 138
EaeI y'GGCC_r
Cuts at: 0 211 1505 1572
Size: 211 1294 67
Fragments arranged by size:
1294 211 67
EarI CTCTTCn'nnn_
Cuts at: 0 128 521 683 1555 1572
Size: 128 393 162 872 17
Fragments arranged by size:
872 393 162 128 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 17 95 296 1372 1572
Size: 17 78 201 1076 200
Fragments arranged by size:
1076 201 200 78 17
EcoO109I rG'GnC_Cy
Cuts at: 0 1349 1572
Size: 1349 223
EcoRI G'AATT_C
Cuts at: 0 280 1572
Size: 280 1292
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 522 554 1264 1296 1572
Size: 76 32 414 32 710 32 276
Fragments arranged by size:
710 414 276 76 32 32 32
HincII GTy'rAC
Cuts at: 0 921 1272 1572
Size: 921 351 300
Fragments arranged by size:
921 351 300
HindIII A'AGCT_T
Cuts at: 0 1253 1572
Size: 1253 319
HpaI GTT'AAC
Cuts at: 0 921 1572
Size: 921 651
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 1191 1572
Size: 419 772 381
Fragments arranged by size:
772 419 381
MscI TGG'CCA
Cuts at: 0 1507 1572
Size: 1507 65
MslI CAynn'nnrTG
Cuts at: 0 289 632 997 1572
Size: 289 343 365 575
Fragments arranged by size:
575 365 343 289
NcoI C'CATG_G
Cuts at: 0 1205 1572
Size: 1205 367
NdeI CA'TA_TG
Cuts at: 0 1276 1572
Size: 1276 296
NspI r_CATG'y
Cuts at: 0 294 631 1081 1572
Size: 294 337 450 491
Fragments arranged by size:
491 450 337 294
PciI A'CATG_T
Cuts at: 0 627 1572
Size: 627 945
SalI G'TCGA_C
Cuts at: 0 1270 1572
Size: 1270 302
SexAI A'CCwGG_T
Cuts at: 0 938 1572
Size: 938 634
SfcI C'TryA_G
Cuts at: 0 316 1572
Size: 316 1256
SmlI C'TyrA_G
Cuts at: 0 1344 1572
Size: 1344 228
SphI G_CATG'C
Cuts at: 0 294 1572
Size: 294 1278
SspI AAT'ATT
Cuts at: 0 1415 1572
Size: 1415 157
StyI C'CwwG_G
Cuts at: 0 476 1205 1572
Size: 476 729 367
Fragments arranged by size:
729 476 367
TatI w'GTAC_w
Cuts at: 0 1119 1572
Size: 1119 453
Enzymes that do cut:
AccI AcuI AflIII AhdI AleI ApoI AseI BanI
BanII BbsI BclI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BsrDI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtsI ClaI EaeI EarI Eco57MI EcoO109I
EcoRI Hin4I HincII HindIII HpaI MmeI MscI MslI
NcoI NdeI NspI PciI SalI SexAI SfcI SmlI
SphI SspI StyI TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AlfI
AloI AlwNI ApaI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsiWI
BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstZ17I BtgZI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoNI EcoRV FalI FseI FspI
FspAI HaeII KasI KpnI MfeI MluI MspA1I NaeI
NarI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI StuI SwaI TaqII
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI