Restriction map for
pFN21AB5877
(Linear) (Six-base) MAPSORT of: KIBB5877.seq Check: 7871 from: 1 to: 1395
KIBB5877.orf
With 182 enzymes: SgfI *
March 22, 2012 15:32 ..
AccI GT'mk_AC
Cuts at: 0 1010 1395
Size: 1010 385
AflIII A'CryG_T
Cuts at: 0 413 1395
Size: 413 982
AleI CACnn'nnGTG
Cuts at: 0 502 1395
Size: 502 893
ApoI r'AATT_y
Cuts at: 0 1326 1395
Size: 1326 69
BanI G'GyrC_C
Cuts at: 0 460 1395
Size: 460 935
BanII G_rGCy'C
Cuts at: 0 1042 1160 1395
Size: 1042 118 235
Fragments arranged by size:
1042 235 118
BbeI G_GCGC'C
Cuts at: 0 464 1395
Size: 464 931
BglI GCCn_nnn'nGGC
Cuts at: 0 94 103 246 400 1395
Size: 94 9 143 154 995
Fragments arranged by size:
995 154 143 94 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 125 1395
Size: 125 1270
BmtI G_CTAG'C
Cuts at: 0 85 1022 1395
Size: 85 937 373
Fragments arranged by size:
937 373 85
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1144 1395
Size: 1144 251
BsaI GGTCTCn'nnnn_
Cuts at: 0 1289 1395
Size: 1289 106
BsaHI Gr'CG_yC
Cuts at: 0 59 392 461 1395
Size: 59 333 69 934
Fragments arranged by size:
934 333 69 59
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 1395
Size: 139 30 1226
Fragments arranged by size:
1226 139 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 144 147 842 1395
Size: 144 3 695 553
Fragments arranged by size:
695 553 144 3
BseYI C'CCAG_C
Cuts at: 0 12 171 203 1395
Size: 12 159 32 1192
Fragments arranged by size:
1192 159 32 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 256 1395
Size: 74 182 1139
Fragments arranged by size:
1139 182 74
BsiHKAI G_wGCw'C
Cuts at: 0 1042 1160 1395
Size: 1042 118 235
Fragments arranged by size:
1042 235 118
Bsp1286I G_dGCh'C
Cuts at: 0 1042 1160 1395
Size: 1042 118 235
Fragments arranged by size:
1042 235 118
BspHI T'CATG_A
Cuts at: 0 861 914 1395
Size: 861 53 481
Fragments arranged by size:
861 481 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 996 1395
Size: 996 399
BsrDI GCAATG_nn'
Cuts at: 0 533 712 775 1040 1395
Size: 533 179 63 265 355
Fragments arranged by size:
533 355 265 179 63
BsrFI r'CCGG_y
Cuts at: 0 1184 1395
Size: 1184 211
BsrGI T'GTAC_A
Cuts at: 0 1049 1199 1395
Size: 1049 150 196
Fragments arranged by size:
1049 196 150
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 1395
Size: 540 855
BtgI C'CryG_G
Cuts at: 0 98 398 1395
Size: 98 300 997
Fragments arranged by size:
997 300 98
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 237 1395
Size: 237 1158
BtsI GCAGTG_nn'
Cuts at: 0 296 962 1395
Size: 296 666 433
Fragments arranged by size:
666 433 296
EaeI y'GGCC_r
Cuts at: 0 2 95 401 530 709 1395
Size: 2 93 306 129 179 686
Fragments arranged by size:
686 306 179 129 93 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1332 1395
Size: 1332 63
EcoICRI GAG'CTC
Cuts at: 0 1040 1158 1395
Size: 1040 118 237
Fragments arranged by size:
1040 237 118
EcoO109I rG'GnC_Cy
Cuts at: 0 718 1395
Size: 718 677
EcoRI G'AATT_C
Cuts at: 0 1326 1395
Size: 1326 69
HaeII r_GCGC'y
Cuts at: 0 464 1395
Size: 464 931
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 818 850 1395
Size: 818 32 545
Fragments arranged by size:
818 545 32
KasI G'GCGC_C
Cuts at: 0 460 1395
Size: 460 935
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 789 1252 1395
Size: 413 376 463 143
Fragments arranged by size:
463 413 376 143
MscI TGG'CCA
Cuts at: 0 4 711 1395
Size: 4 707 684
Fragments arranged by size:
707 684 4
MslI CAynn'nnrTG
Cuts at: 0 502 538 1395
Size: 502 36 857
Fragments arranged by size:
857 502 36
MspA1I CmG'CkG
Cuts at: 0 502 1395
Size: 502 893
NarI GG'CG_CC
Cuts at: 0 461 1395
Size: 461 934
NcoI C'CATG_G
Cuts at: 0 398 1395
Size: 398 997
NheI G'CTAG_C
Cuts at: 0 81 1018 1395
Size: 81 937 377
Fragments arranged by size:
937 377 81
PasI CC'CwG_GG
Cuts at: 0 209 1395
Size: 209 1186
PpuMI rG'GwC_Cy
Cuts at: 0 718 1395
Size: 718 677
PvuII CAG'CTG
Cuts at: 0 502 1395
Size: 502 893
SacI G_AGCT'C
Cuts at: 0 1042 1160 1395
Size: 1042 118 235
Fragments arranged by size:
1042 235 118
SexAI A'CCwGG_T
Cuts at: 0 1168 1261 1395
Size: 1168 93 134
Fragments arranged by size:
1168 134 93
SfcI C'TryA_G
Cuts at: 0 590 1395
Size: 590 805
SfoI GGC'GCC
Cuts at: 0 462 1395
Size: 462 933
SmlI C'TyrA_G
Cuts at: 0 1159 1395
Size: 1159 236
SspI AAT'ATT
Cuts at: 0 1390 1395
Size: 1390 5
StyI C'CwwG_G
Cuts at: 0 398 1395
Size: 398 997
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 40 107 1395
Size: 40 67 1288
Fragments arranged by size:
1288 67 40
TatI w'GTAC_w
Cuts at: 0 764 1049 1199 1395
Size: 764 285 150 196
Fragments arranged by size:
764 285 196 150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 167 199 1395
Size: 167 32 1196
Fragments arranged by size:
1196 167 32
Enzymes that do cut:
AccI AflIII AleI ApoI BanI BanII BbeI BglI
BmrI BmtI BpuEI BsaI BsaHI BsaXI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI
BstXI BtgI BtgZI BtsI EaeI EciI EcoICRI EcoO109I
EcoRI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NarI NcoI NheI PasI PpuMI PvuII SacI SexAI
SfcI SfoI SmlI SspI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BsaAI BsaBI BsaWI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI
EagI EarI Eco57MI EcoNI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI