Restriction map for
pFN21AB5844
(Linear) (Six-base) MAPSORT of: KIBB5844.seq Check: 8981 from: 1 to: 1452
KIBB5844.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 758 1452
Size: 758 694
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 635 1426 1452
Size: 391 244 791 26
Fragments arranged by size:
791 391 244 26
AflIII A'CryG_T
Cuts at: 0 440 1452
Size: 440 1012
AhdI GACnn_n'nnGTC
Cuts at: 0 365 480 1452
Size: 365 115 972
Fragments arranged by size:
972 365 115
AleI CACnn'nnGTG
Cuts at: 0 1234 1452
Size: 1234 218
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1183 1452
Size: 1183 269
ApaI G_GGCC'C
Cuts at: 0 1020 1452
Size: 1020 432
ApoI r'AATT_y
Cuts at: 0 518 1114 1295 1363 1452
Size: 518 596 181 68 89
Fragments arranged by size:
596 518 181 89 68
AvaI C'yCGr_G
Cuts at: 0 93 253 844 946 1020 1452
Size: 93 160 591 102 74 432
Fragments arranged by size:
591 432 160 102 93 74
BanI G'GyrC_C
Cuts at: 0 22 1243 1452
Size: 22 1221 209
Fragments arranged by size:
1221 209 22
BanII G_rGCy'C
Cuts at: 0 238 1020 1137 1452
Size: 238 782 117 315
Fragments arranged by size:
782 315 238 117
BbeI G_GCGC'C
Cuts at: 0 26 1247 1452
Size: 26 1221 205
Fragments arranged by size:
1221 205 26
BbvCI CC'TCA_GC
Cuts at: 0 986 1452
Size: 986 466
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 858 892 1452
Size: 858 34 560
Fragments arranged by size:
858 560 34
BglI GCCn_nnn'nGGC
Cuts at: 0 70 1157 1452
Size: 70 1087 295
Fragments arranged by size:
1087 295 70
Bme1580I G_kGCm'C
Cuts at: 0 1020 1452
Size: 1020 432
BmgBI CAC'GTC
Cuts at: 0 1428 1452
Size: 1428 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 392 1452
Size: 392 1060
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 642 1452
Size: 642 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 577 679 1340 1452
Size: 139 438 102 661 112
Fragments arranged by size:
661 438 139 112 102
Bpu10I CC'TnA_GC
Cuts at: 0 832 986 1452
Size: 832 154 466
Fragments arranged by size:
832 466 154
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1117 1452
Size: 1117 335
BsaI GGTCTCn'nnnn_
Cuts at: 0 718 922 1416 1452
Size: 718 204 494 36
Fragments arranged by size:
718 494 204 36
BsaAI yAC'GTr
Cuts at: 0 441 1452
Size: 441 1011
BsaHI Gr'CG_yC
Cuts at: 0 23 1244 1452
Size: 23 1221 208
Fragments arranged by size:
1221 208 23
BsaWI w'CCGG_w
Cuts at: 0 1129 1452
Size: 1129 323
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 951 1273 1452
Size: 951 322 179
Fragments arranged by size:
951 322 179
BseYI C'CCAG_C
Cuts at: 0 632 1452
Size: 632 820
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 583 594 1452
Size: 85 498 11 858
Fragments arranged by size:
858 498 85 11
BsiEI CG_ry'CG
Cuts at: 0 1014 1452
Size: 1014 438
BsiHKAI G_wGCw'C
Cuts at: 0 260 1452
Size: 260 1192
Bsp1286I G_dGCh'C
Cuts at: 0 238 260 1020 1137 1452
Size: 238 22 760 117 315
Fragments arranged by size:
760 315 238 117 22
BspEI T'CCGG_A
Cuts at: 0 1129 1452
Size: 1129 323
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 758 1452
Size: 758 694
BsrBI CCG'CTC
Cuts at: 0 153 1452
Size: 153 1299
BsrFI r'CCGG_y
Cuts at: 0 836 1452
Size: 836 616
BsrGI T'GTAC_A
Cuts at: 0 218 1452
Size: 218 1234
BstAPI GCAn_nnn'nTGC
Cuts at: 0 531 561 1452
Size: 531 30 891
Fragments arranged by size:
891 531 30
BstEII G'GTnAC_C
Cuts at: 0 159 1452
Size: 159 1293
BstXI CCAn_nnnn'nTGG
Cuts at: 0 404 601 1236 1452
Size: 404 197 635 216
Fragments arranged by size:
635 404 216 197
BstYI r'GATC_y
Cuts at: 0 920 1452
Size: 920 532
Bsu36I CC'TnA_GG
Cuts at: 0 934 1153 1452
Size: 934 219 299
Fragments arranged by size:
934 299 219
BtgI C'CryG_G
Cuts at: 0 1055 1229 1452
Size: 1055 174 223
Fragments arranged by size:
1055 223 174
BtsI GCAGTG_nn'
Cuts at: 0 474 643 1452
Size: 474 169 809
Fragments arranged by size:
809 474 169
ClaI AT'CG_AT
Cuts at: 0 740 1452
Size: 740 712
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 780 815 1452
Size: 780 35 637
Fragments arranged by size:
780 637 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 307 1452
Size: 307 1145
EaeI y'GGCC_r
Cuts at: 0 1058 1211 1452
Size: 1058 153 241
Fragments arranged by size:
1058 241 153
EarI CTCTTCn'nnn_
Cuts at: 0 367 611 1304 1452
Size: 367 244 693 148
Fragments arranged by size:
693 367 244 148
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1452
Size: 19 1433
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 391 577 635 679 1340 1426 1452
Size: 139 252 186 58 44 661 86 26
Fragments arranged by size:
661 252 186 139 86 58 44 26
EcoO109I rG'GnC_Cy
Cuts at: 0 543 1017 1452
Size: 543 474 435
Fragments arranged by size:
543 474 435
EcoRI G'AATT_C
Cuts at: 0 518 1363 1452
Size: 518 845 89
Fragments arranged by size:
845 518 89
HaeII r_GCGC'y
Cuts at: 0 26 1247 1452
Size: 26 1221 205
Fragments arranged by size:
1221 205 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 466 498 935 967 1092 1124
Size: 351 32 83 32 437 32 125 32
Cuts at: 1124 1452
Size: 328
Fragments arranged by size:
437 351 328 125 83 32 32 32
32
HincII GTy'rAC
Cuts at: 0 359 765 1304 1452
Size: 359 406 539 148
Fragments arranged by size:
539 406 359 148
KasI G'GCGC_C
Cuts at: 0 22 1243 1452
Size: 22 1221 209
Fragments arranged by size:
1221 209 22
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 815 1310 1452
Size: 815 495 142
Fragments arranged by size:
815 495 142
MscI TGG'CCA
Cuts at: 0 1213 1452
Size: 1213 239
MslI CAynn'nnrTG
Cuts at: 0 402 1234 1452
Size: 402 832 218
Fragments arranged by size:
832 402 218
MspA1I CmG'CkG
Cuts at: 0 990 1388 1452
Size: 990 398 64
Fragments arranged by size:
990 398 64
NaeI GCC'GGC
Cuts at: 0 838 1452
Size: 838 614
NarI GG'CG_CC
Cuts at: 0 23 1244 1452
Size: 23 1221 208
Fragments arranged by size:
1221 208 23
NgoMIV G'CCGG_C
Cuts at: 0 836 1452
Size: 836 616
NspI r_CATG'y
Cuts at: 0 219 531 714 1452
Size: 219 312 183 738
Fragments arranged by size:
738 312 219 183
PfoI T'CCnGG_A
Cuts at: 0 656 923 1452
Size: 656 267 529
Fragments arranged by size:
656 529 267
PpuMI rG'GwC_Cy
Cuts at: 0 543 1452
Size: 543 909
PspOMI G'GGCC_C
Cuts at: 0 1016 1452
Size: 1016 436
PstI C_TGCA'G
Cuts at: 0 469 1452
Size: 469 983
PvuII CAG'CTG
Cuts at: 0 1388 1452
Size: 1388 64
SapI GCTCTTCn'nnn_
Cuts at: 0 611 1452
Size: 611 841
ScaI AGT'ACT
Cuts at: 0 178 1452
Size: 178 1274
SfcI C'TryA_G
Cuts at: 0 465 1452
Size: 465 987
SfoI GGC'GCC
Cuts at: 0 24 1245 1452
Size: 24 1221 207
Fragments arranged by size:
1221 207 24
SmaI CCC'GGG
Cuts at: 0 95 1452
Size: 95 1357
SmlI C'TyrA_G
Cuts at: 0 946 1096 1452
Size: 946 150 356
Fragments arranged by size:
946 356 150
StyI C'CwwG_G
Cuts at: 0 200 1452
Size: 200 1252
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 175 1321 1452
Size: 175 1146 131
Fragments arranged by size:
1146 175 131
TatI w'GTAC_w
Cuts at: 0 176 218 1382 1452
Size: 176 42 1164 70
Fragments arranged by size:
1164 176 70 42
XhoI C'TCGA_G
Cuts at: 0 946 1452
Size: 946 506
XmaI C'CCGG_G
Cuts at: 0 93 1452
Size: 93 1359
XmnI GAAnn'nnTTC
Cuts at: 0 460 1452
Size: 460 992
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI ApaI ApoI
AvaI BanI BanII BbeI BbvCI BcgI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI ClaI CspCI DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PfoI PpuMI PspOMI PstI PvuII SapI ScaI
SfcI SfoI SmaI SmlI StyI TaqII TatI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BciVI BclI BfrBI BglII BlpI BmtI BsaBI
BsaXI BsiWI BsmI BsmBI BspHI BsrDI BssHII BssSI
BstBI BstZ17I BtgZI DraI DraIII EagI EcoICRI EcoNI
EcoRV FalI FseI FspI FspAI HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SbfI SexAI SfiI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI Tth111I XbaI
XcmI ZraI