Restriction map for
pFN21AB5820
(Linear) (Six-base) MAPSORT of: KIBB5820.seq Check: 7201 from: 1 to: 1593
KIBB5820.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 252 1593
Size: 252 1341
AccI GT'mk_AC
Cuts at: 0 1184 1593
Size: 1184 409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1267 1369 1593
Size: 1071 196 102 224
Fragments arranged by size:
1071 224 196 102
AleI CACnn'nnGTG
Cuts at: 0 1450 1593
Size: 1450 143
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 1550 1593
Size: 916 634 43
Fragments arranged by size:
916 634 43
AlwNI CAG_nnn'CTG
Cuts at: 0 640 1002 1563 1575 1593
Size: 640 362 561 12 18
Fragments arranged by size:
640 561 362 18 12
ApaI G_GGCC'C
Cuts at: 0 160 190 199 1509 1593
Size: 160 30 9 1310 84
Fragments arranged by size:
1310 160 84 30 9
ApaLI G'TGCA_C
Cuts at: 0 891 967 1593
Size: 891 76 626
Fragments arranged by size:
891 626 76
ApoI r'AATT_y
Cuts at: 0 1580 1593
Size: 1580 13
AvaI C'yCGr_G
Cuts at: 0 160 1593
Size: 160 1433
BamHI G'GATC_C
Cuts at: 0 336 1593
Size: 336 1257
BanI G'GyrC_C
Cuts at: 0 55 821 1593
Size: 55 766 772
Fragments arranged by size:
772 766 55
BanII G_rGCy'C
Cuts at: 0 160 190 199 1509 1593
Size: 160 30 9 1310 84
Fragments arranged by size:
1310 160 84 30 9
BbeI G_GCGC'C
Cuts at: 0 59 1593
Size: 59 1534
BbsI GAAGACnn'nnnn_
Cuts at: 0 1210 1357 1593
Size: 1210 147 236
Fragments arranged by size:
1210 236 147
BbvCI CC'TCA_GC
Cuts at: 0 437 1593
Size: 437 1156
BglI GCCn_nnn'nGGC
Cuts at: 0 50 185 194 1593
Size: 50 135 9 1399
Fragments arranged by size:
1399 135 50 9
BglII A'GATC_T
Cuts at: 0 1082 1593
Size: 1082 511
BlpI GC'TnA_GC
Cuts at: 0 40 1593
Size: 40 1553
Bme1580I G_kGCm'C
Cuts at: 0 160 190 199 365 895 971 1043 1509
Size: 160 30 9 166 530 76 72 466
Cuts at: 1509 1593
Size: 84
Fragments arranged by size:
530 466 166 160 84 76 72 30
9
BmrI ACTGGGnnnn_n'
Cuts at: 0 921 1593
Size: 921 672
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1317 1593
Size: 1317 276
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1593
Size: 893 700
Bpu10I CC'TnA_GC
Cuts at: 0 437 1593
Size: 437 1156
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 1593
Size: 1420 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 869 1318 1424 1593
Size: 869 449 106 169
Fragments arranged by size:
869 449 169 106
BsaBI GATnn'nnATC
Cuts at: 0 535 1593
Size: 535 1058
BsaHI Gr'CG_yC
Cuts at: 0 56 1593
Size: 56 1537
BsaWI w'CCGG_w
Cuts at: 0 239 824 1593
Size: 239 585 769
Fragments arranged by size:
769 585 239
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 107 137 1593
Size: 107 30 1456
Fragments arranged by size:
1456 107 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 423 1483 1486 1593
Size: 423 1060 3 107
Fragments arranged by size:
1060 423 107 3
BseYI C'CCAG_C
Cuts at: 0 389 610 663 960 1421 1575 1593
Size: 389 221 53 297 461 154 18
Fragments arranged by size:
461 389 297 221 154 53 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 653 1564 1593
Size: 314 339 911 29
Fragments arranged by size:
911 339 314 29
BsiHKAI G_wGCw'C
Cuts at: 0 895 971 1593
Size: 895 76 622
Fragments arranged by size:
895 622 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1470 1593
Size: 1470 123
Bsp1286I G_dGCh'C
Cuts at: 0 160 190 199 365 895 971 1043 1509
Size: 160 30 9 166 530 76 72 466
Cuts at: 1509 1593
Size: 84
Fragments arranged by size:
530 466 166 160 84 76 72 30
9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 252 1593
Size: 252 1341
BsrBI CCG'CTC
Cuts at: 0 498 1047 1593
Size: 498 549 546
Fragments arranged by size:
549 546 498
BsrDI GCAATG_nn'
Cuts at: 0 515 1593
Size: 515 1078
BsrFI r'CCGG_y
Cuts at: 0 117 1593
Size: 117 1476
BssSI C'ACGA_G
Cuts at: 0 1042 1593
Size: 1042 551
BstAPI GCAn_nnn'nTGC
Cuts at: 0 640 828 1593
Size: 640 188 765
Fragments arranged by size:
765 640 188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 220 333 1128 1593
Size: 220 113 795 465
Fragments arranged by size:
795 465 220 113
BstYI r'GATC_y
Cuts at: 0 336 480 536 584 1082 1316 1593
Size: 336 144 56 48 498 234 277
Fragments arranged by size:
498 336 277 234 144 56 48
Bsu36I CC'TnA_GG
Cuts at: 0 1466 1593
Size: 1466 127
BtgI C'CryG_G
Cuts at: 0 59 92 182 191 200 248 1593
Size: 59 33 90 9 9 48 1345
Fragments arranged by size:
1345 90 59 48 33 9 9
ClaI AT'CG_AT
Cuts at: 0 1223 1593
Size: 1223 370
DraIII CAC_nnn'GTG
Cuts at: 0 813 1593
Size: 813 780
DrdI GACnn_nn'nnGTC
Cuts at: 0 883 1159 1332 1593
Size: 883 276 173 261
Fragments arranged by size:
883 276 261 173
EaeI y'GGCC_r
Cuts at: 0 1095 1593
Size: 1095 498
EarI CTCTTCn'nnn_
Cuts at: 0 730 1593
Size: 730 863
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 166 634 1066 1593
Size: 166 468 432 527
Fragments arranged by size:
527 468 432 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1071 1267 1369 1593
Size: 893 178 196 102 224
Fragments arranged by size:
893 224 196 178 102
EcoO109I rG'GnC_Cy
Cuts at: 0 97 156 157 187 196 268 647 999
Size: 97 59 1 30 9 72 379 352
Cuts at: 999 1593
Size: 594
Fragments arranged by size:
594 379 352 97 72 59 30 9
1
EcoRI G'AATT_C
Cuts at: 0 1580 1593
Size: 1580 13
HaeII r_GCGC'y
Cuts at: 0 59 82 124 396 1428 1593
Size: 59 23 42 272 1032 165
Fragments arranged by size:
1032 272 165 59 42 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 107 139 504 536 1446 1478 1593
Size: 107 32 365 32 910 32 115
Fragments arranged by size:
910 365 115 107 32 32 32
HincII GTy'rAC
Cuts at: 0 1185 1593
Size: 1185 408
HindIII A'AGCT_T
Cuts at: 0 577 1593
Size: 577 1016
KasI G'GCGC_C
Cuts at: 0 55 1593
Size: 55 1538
MslI CAynn'nnrTG
Cuts at: 0 331 1225 1450 1593
Size: 331 894 225 143
Fragments arranged by size:
894 331 225 143
MspA1I CmG'CkG
Cuts at: 0 30 94 193 202 1029 1347 1593
Size: 30 64 99 9 827 318 246
Fragments arranged by size:
827 318 246 99 64 30 9
NarI GG'CG_CC
Cuts at: 0 56 1593
Size: 56 1537
NcoI C'CATG_G
Cuts at: 0 182 248 1593
Size: 182 66 1345
Fragments arranged by size:
1345 182 66
NspI r_CATG'y
Cuts at: 0 108 228 321 1593
Size: 108 120 93 1272
Fragments arranged by size:
1272 120 108 93
PasI CC'CwG_GG
Cuts at: 0 72 652 1502 1593
Size: 72 580 850 91
Fragments arranged by size:
850 580 91 72
PfoI T'CCnGG_A
Cuts at: 0 446 539 1403 1593
Size: 446 93 864 190
Fragments arranged by size:
864 446 190 93
PpuMI rG'GwC_Cy
Cuts at: 0 97 268 647 1593
Size: 97 171 379 946
Fragments arranged by size:
946 379 171 97
PspOMI G'GGCC_C
Cuts at: 0 156 186 195 1505 1593
Size: 156 30 9 1310 88
Fragments arranged by size:
1310 156 88 30 9
PstI C_TGCA'G
Cuts at: 0 645 1034 1145 1593
Size: 645 389 111 448
Fragments arranged by size:
645 448 389 111
PvuII CAG'CTG
Cuts at: 0 1029 1347 1593
Size: 1029 318 246
Fragments arranged by size:
1029 318 246
RsrII CG'GwC_CG
Cuts at: 0 115 1593
Size: 115 1478
SacII CC_GC'GG
Cuts at: 0 95 194 203 1593
Size: 95 99 9 1390
Fragments arranged by size:
1390 99 95 9
SalI G'TCGA_C
Cuts at: 0 1183 1593
Size: 1183 410
SanDI GG'GwC_CC
Cuts at: 0 97 268 647 1593
Size: 97 171 379 946
Fragments arranged by size:
946 379 171 97
SexAI A'CCwGG_T
Cuts at: 0 809 1593
Size: 809 784
SfcI C'TryA_G
Cuts at: 0 641 1030 1141 1593
Size: 641 389 111 452
Fragments arranged by size:
641 452 389 111
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 194 1593
Size: 194 1399
SfoI GGC'GCC
Cuts at: 0 57 1593
Size: 57 1536
SmaI CCC'GGG
Cuts at: 0 162 1593
Size: 162 1431
SmlI C'TyrA_G
Cuts at: 0 1435 1593
Size: 1435 158
SphI G_CATG'C
Cuts at: 0 108 321 1593
Size: 108 213 1272
Fragments arranged by size:
1272 213 108
StyI C'CwwG_G
Cuts at: 0 182 248 1538 1593
Size: 182 66 1290 55
Fragments arranged by size:
1290 182 66 55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 853 1593
Size: 339 514 740
Fragments arranged by size:
740 514 339
TatI w'GTAC_w
Cuts at: 0 929 1052 1593
Size: 929 123 541
Fragments arranged by size:
929 541 123
Tth111I GACn'n_nGTC
Cuts at: 0 134 1593
Size: 134 1459
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 847 1033 1593
Size: 847 186 560
Fragments arranged by size:
847 560 186
XmaI C'CCGG_G
Cuts at: 0 160 1593
Size: 160 1433
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI ClaI DraIII
DrdI EaeI EarI EciI Eco57MI EcoO109I EcoRI HaeII
Hin4I HincII HindIII KasI MslI MspA1I NarI NcoI
NspI PasI PfoI PpuMI PspOMI PstI PvuII RsrII
SacII SalI SanDI SexAI SfcI SfiI SfoI SmaI
SmlI SphI StyI TaqII TatI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BmgBI BmtI BsaAI BsiEI BsiWI BsmI
BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I BtgZI
BtsI CspCI DraI EagI EcoICRI EcoNI EcoRV FalI
FseI FspI FspAI HpaI KpnI MfeI MluI MmeI
MscI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI SacI SapI SbfI ScaI SgfI
SgrAI SnaBI SpeI SrfI SspI StuI SwaI TstI
XbaI XhoI XmnI ZraI