Restriction map for
pFN21AB5806
(Linear) (Six-base) MAPSORT of: KIBB5806.seq Check: 321 from: 1 to: 1356
KIBB5806.orf
With 182 enzymes: SgfI *
March 22, 2012 15:32 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 1356
Size: 1298 58
AleI CACnn'nnGTG
Cuts at: 0 607 723 1356
Size: 607 116 633
Fragments arranged by size:
633 607 116
AlwNI CAG_nnn'CTG
Cuts at: 0 1321 1356
Size: 1321 35
ApaI G_GGCC'C
Cuts at: 0 497 1356
Size: 497 859
ApoI r'AATT_y
Cuts at: 0 1039 1356
Size: 1039 317
AvaI C'yCGr_G
Cuts at: 0 902 1356
Size: 902 454
BamHI G'GATC_C
Cuts at: 0 1078 1356
Size: 1078 278
BanI G'GyrC_C
Cuts at: 0 154 601 707 1356
Size: 154 447 106 649
Fragments arranged by size:
649 447 154 106
BanII G_rGCy'C
Cuts at: 0 329 497 541 1356
Size: 329 168 44 815
Fragments arranged by size:
815 329 168 44
BbeI G_GCGC'C
Cuts at: 0 711 1356
Size: 711 645
BbsI GAAGACnn'nnnn_
Cuts at: 0 843 1356
Size: 843 513
BbvCI CC'TCA_GC
Cuts at: 0 695 1356
Size: 695 661
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 883 917 1356
Size: 883 34 439
Fragments arranged by size:
883 439 34
BclI T'GATC_A
Cuts at: 0 1142 1356
Size: 1142 214
Bme1580I G_kGCm'C
Cuts at: 0 497 1356
Size: 497 859
BmrI ACTGGGnnnn_n'
Cuts at: 0 546 1084 1356
Size: 546 538 272
Fragments arranged by size:
546 538 272
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1068 1356
Size: 109 959 288
Fragments arranged by size:
959 288 109
Bpu10I CC'TnA_GC
Cuts at: 0 695 1356
Size: 695 661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1074 1356
Size: 349 725 282
Fragments arranged by size:
725 349 282
BsaHI Gr'CG_yC
Cuts at: 0 263 708 1356
Size: 263 445 648
Fragments arranged by size:
648 445 263
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 538 568 1356
Size: 538 30 788
Fragments arranged by size:
788 538 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 114 117 1356
Size: 114 3 1239
Fragments arranged by size:
1239 114 3
BseYI C'CCAG_C
Cuts at: 0 399 496 1356
Size: 399 97 860
Fragments arranged by size:
860 399 97
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1196 1356
Size: 331 865 160
Fragments arranged by size:
865 331 160
BsiEI CG_ry'CG
Cuts at: 0 251 1356
Size: 251 1105
BsmI GAATG_Cn'
Cuts at: 0 470 1356
Size: 470 886
Bsp1286I G_dGCh'C
Cuts at: 0 329 497 541 1356
Size: 329 168 44 815
Fragments arranged by size:
815 329 168 44
BspHI T'CATG_A
Cuts at: 0 440 1356
Size: 440 916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 353 1356
Size: 353 1003
BsrBI CCG'CTC
Cuts at: 0 204 1258 1356
Size: 204 1054 98
Fragments arranged by size:
1054 204 98
BsrDI GCAATG_nn'
Cuts at: 0 689 1356
Size: 689 667
BsrFI r'CCGG_y
Cuts at: 0 151 1356
Size: 151 1205
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1174 1356
Size: 1174 182
BstEII G'GTnAC_C
Cuts at: 0 684 1356
Size: 684 672
BstYI r'GATC_y
Cuts at: 0 311 1078 1356
Size: 311 767 278
Fragments arranged by size:
767 311 278
BtgI C'CryG_G
Cuts at: 0 318 447 515 1356
Size: 318 129 68 841
Fragments arranged by size:
841 318 129 68
EaeI y'GGCC_r
Cuts at: 0 248 509 573 645 1269 1356
Size: 248 261 64 72 624 87
Fragments arranged by size:
624 261 248 87 72 64
EagI C'GGCC_G
Cuts at: 0 248 1356
Size: 248 1108
EarI CTCTTCn'nnn_
Cuts at: 0 588 756 1356
Size: 588 168 600
Fragments arranged by size:
600 588 168
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1268 1276 1356
Size: 1268 8 80
Fragments arranged by size:
1268 80 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1068 1298 1356
Size: 109 959 230 58
Fragments arranged by size:
959 230 109 58
EcoO109I rG'GnC_Cy
Cuts at: 0 493 1356
Size: 493 863
EcoRI G'AATT_C
Cuts at: 0 1039 1356
Size: 1039 317
HaeII r_GCGC'y
Cuts at: 0 364 711 1356
Size: 364 347 645
Fragments arranged by size:
645 364 347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 253 285 1356
Size: 151 32 70 32 1071
Fragments arranged by size:
1071 151 70 32 32
HincII GTy'rAC
Cuts at: 0 5 615 1356
Size: 5 610 741
Fragments arranged by size:
741 610 5
KasI G'GCGC_C
Cuts at: 0 707 1356
Size: 707 649
MscI TGG'CCA
Cuts at: 0 575 1271 1356
Size: 575 696 85
Fragments arranged by size:
696 575 85
MslI CAynn'nnrTG
Cuts at: 0 607 723 1356
Size: 607 116 633
Fragments arranged by size:
633 607 116
MspA1I CmG'CkG
Cuts at: 0 517 788 1356
Size: 517 271 568
Fragments arranged by size:
568 517 271
NarI GG'CG_CC
Cuts at: 0 708 1356
Size: 708 648
NcoI C'CATG_G
Cuts at: 0 447 1356
Size: 447 909
NdeI CA'TA_TG
Cuts at: 0 974 1356
Size: 974 382
NspI r_CATG'y
Cuts at: 0 1282 1356
Size: 1282 74
PfoI T'CCnGG_A
Cuts at: 0 15 1356
Size: 15 1341
PspOMI G'GGCC_C
Cuts at: 0 493 1356
Size: 493 863
SacII CC_GC'GG
Cuts at: 0 518 1356
Size: 518 838
SapI GCTCTTCn'nnn_
Cuts at: 0 756 1356
Size: 756 600
ScaI AGT'ACT
Cuts at: 0 301 1356
Size: 301 1055
SfoI GGC'GCC
Cuts at: 0 709 1356
Size: 709 647
SmlI C'TyrA_G
Cuts at: 0 364 1053 1356
Size: 364 689 303
Fragments arranged by size:
689 364 303
SpeI A'CTAG_T
Cuts at: 0 21 1356
Size: 21 1335
StyI C'CwwG_G
Cuts at: 0 447 576 872 1272 1356
Size: 447 129 296 400 84
Fragments arranged by size:
447 400 296 129 84
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 485 1356
Size: 485 871
TatI w'GTAC_w
Cuts at: 0 194 299 1356
Size: 194 105 1057
Fragments arranged by size:
1057 194 105
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 199 231 538 570 1356
Size: 199 32 307 32 786
Fragments arranged by size:
786 307 199 32 32
XbaI T'CTAG_A
Cuts at: 0 1101 1356
Size: 1101 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 504 972 1356
Size: 504 468 384
Fragments arranged by size:
504 468 384
Enzymes that do cut:
AcuI AleI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BclI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI
BstAPI BstEII BstYI BtgI EaeI EagI EarI EciI
Eco57MI EcoO109I EcoRI HaeII Hin4I HincII KasI MscI
MslI MspA1I NarI NcoI NdeI NspI PfoI PspOMI
SacII SapI ScaI SfoI SmlI SpeI StyI TaqII
TatI TstI XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BciVI BfrBI BglI BglII BlpI BmgBI
BmtI BplI BsaI BsaAI BsaBI BsaWI BsiHKAI BsiWI
BsmBI BspEI BsrGI BssHII BssSI BstBI BstXI BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EcoICRI EcoNI EcoRV FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NaeI NgoMIV NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SalI SanDI SbfI SexAI
SfcI SfiI SgfI SgrAI SmaI SnaBI SphI SrfI
SspI StuI SwaI Tth111I XhoI XmaI XmnI ZraI