Restriction map for
pFN21AB5803
(Linear) (Six-base) MAPSORT of: KIBB5803.seq Check: 6005 from: 1 to: 1350
KIBB5803.orf
With 182 enzymes: SgfI *
July 3, 2012 16:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 433 681 1350
Size: 247 186 248 669
Fragments arranged by size:
669 248 247 186
AflII C'TTAA_G
Cuts at: 0 1113 1350
Size: 1113 237
AlwNI CAG_nnn'CTG
Cuts at: 0 462 1350
Size: 462 888
ApoI r'AATT_y
Cuts at: 0 431 470 541 634 976 1014 1350
Size: 431 39 71 93 342 38 336
Fragments arranged by size:
431 342 336 93 71 39 38
AseI AT'TA_AT
Cuts at: 0 362 752 1350
Size: 362 390 598
Fragments arranged by size:
598 390 362
BanI G'GyrC_C
Cuts at: 0 142 1350
Size: 142 1208
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 697 1350
Size: 50 647 653
Fragments arranged by size:
653 647 50
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 51 85 1350
Size: 51 34 1265
Fragments arranged by size:
1265 51 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 115 1350
Size: 115 1235
BglII A'GATC_T
Cuts at: 0 556 1350
Size: 556 794
BmgBI CAC'GTC
Cuts at: 0 1169 1350
Size: 1169 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 1201 1350
Size: 130 1071 149
Fragments arranged by size:
1071 149 130
Bpu10I CC'TnA_GC
Cuts at: 0 1256 1350
Size: 1256 94
BsmI GAATG_Cn'
Cuts at: 0 41 1350
Size: 41 1309
BsrDI GCAATG_nn'
Cuts at: 0 540 636 1350
Size: 540 96 714
Fragments arranged by size:
714 540 96
BsrGI T'GTAC_A
Cuts at: 0 643 1222 1350
Size: 643 579 128
Fragments arranged by size:
643 579 128
BstYI r'GATC_y
Cuts at: 0 556 1350
Size: 556 794
BtsI GCAGTG_nn'
Cuts at: 0 368 679 1350
Size: 368 311 671
Fragments arranged by size:
671 368 311
EaeI y'GGCC_r
Cuts at: 0 578 1350
Size: 578 772
EarI CTCTTCn'nnn_
Cuts at: 0 437 1350
Size: 437 913
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 247 433 681 1201 1350
Size: 130 117 186 248 520 149
Fragments arranged by size:
520 248 186 149 130 117
EcoRI G'AATT_C
Cuts at: 0 431 541 1350
Size: 431 110 809
Fragments arranged by size:
809 431 110
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 238 562 1350
Size: 238 324 788
Fragments arranged by size:
788 324 238
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 770 802 859 891 1158 1190 1350
Size: 770 32 57 32 267 32 160
Fragments arranged by size:
770 267 160 57 32 32 32
HindIII A'AGCT_T
Cuts at: 0 658 1350
Size: 658 692
MfeI C'AATT_G
Cuts at: 0 938 1350
Size: 938 412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 1219 1350
Size: 812 407 131
Fragments arranged by size:
812 407 131
MscI TGG'CCA
Cuts at: 0 580 1350
Size: 580 770
NspI r_CATG'y
Cuts at: 0 592 1350
Size: 592 758
PasI CC'CwG_GG
Cuts at: 0 18 1350
Size: 18 1332
PfoI T'CCnGG_A
Cuts at: 0 107 1350
Size: 107 1243
PstI C_TGCA'G
Cuts at: 0 64 906 1350
Size: 64 842 444
Fragments arranged by size:
842 444 64
SapI GCTCTTCn'nnn_
Cuts at: 0 437 1350
Size: 437 913
SfcI C'TryA_G
Cuts at: 0 60 902 1350
Size: 60 842 448
Fragments arranged by size:
842 448 60
SmlI C'TyrA_G
Cuts at: 0 1113 1350
Size: 1113 237
StyI C'CwwG_G
Cuts at: 0 546 1350
Size: 546 804
TatI w'GTAC_w
Cuts at: 0 643 680 1222 1350
Size: 643 37 542 128
Fragments arranged by size:
643 542 128 37
XmnI GAAnn'nnTTC
Cuts at: 0 197 316 363 446 694 824 1274 1350
Size: 197 119 47 83 248 130 450 76
Fragments arranged by size:
450 248 197 130 119 83 76 47
Enzymes that do cut:
AcuI AflII AlwNI ApoI AseI BanI BbsI BcgI
BciVI BglII BmgBI BpmI Bpu10I BsmI BsrDI BsrGI
BstYI BtsI EaeI EarI Eco57MI EcoRI FalI Hin4I
HindIII MfeI MmeI MscI NspI PasI PfoI PstI
SapI SfcI SmlI StyI TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanII BbeI BbvCI BclI
BfrBI BglI BlpI Bme1580I BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV
FseI FspI FspAI HaeII HincII HpaI KasI KpnI
MluI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TstI
Tth111I XbaI XcmI XhoI XmaI ZraI