Restriction map for
pFN21AB5802
(Linear) (Six-base) MAPSORT of: KIBB5802.seq Check: 3156 from: 1 to: 1413
KIBB5802.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 140 1136 1413
Size: 140 996 277
Fragments arranged by size:
996 277 140
Acc65I G'GTAC_C
Cuts at: 0 413 1413
Size: 413 1000
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 854 1100 1379 1413
Size: 854 246 279 34
Fragments arranged by size:
854 279 246 34
AgeI A'CCGG_T
Cuts at: 0 416 1413
Size: 416 997
AleI CACnn'nnGTG
Cuts at: 0 189 1413
Size: 189 1224
ApaI G_GGCC'C
Cuts at: 0 1080 1413
Size: 1080 333
ApoI r'AATT_y
Cuts at: 0 1290 1413
Size: 1290 123
BanI G'GyrC_C
Cuts at: 0 176 413 1007 1413
Size: 176 237 594 406
Fragments arranged by size:
594 406 237 176
BanII G_rGCy'C
Cuts at: 0 37 1080 1413
Size: 37 1043 333
Fragments arranged by size:
1043 333 37
BbsI GAAGACnn'nnnn_
Cuts at: 0 860 975 1413
Size: 860 115 438
Fragments arranged by size:
860 438 115
BclI T'GATC_A
Cuts at: 0 752 1413
Size: 752 661
BfrBI ATG'CAT
Cuts at: 0 124 1413
Size: 124 1289
Bme1580I G_kGCm'C
Cuts at: 0 1012 1080 1413
Size: 1012 68 333
Fragments arranged by size:
1012 333 68
BmgBI CAC'GTC
Cuts at: 0 465 1413
Size: 465 948
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 196 546 766 1413
Size: 122 74 350 220 647
Fragments arranged by size:
647 350 220 122 74
BsaWI w'CCGG_w
Cuts at: 0 416 1413
Size: 416 997
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 194 224 895 925 1413
Size: 194 30 671 30 488
Fragments arranged by size:
671 488 194 30 30
BseYI C'CCAG_C
Cuts at: 0 366 972 1413
Size: 366 606 441
Fragments arranged by size:
606 441 366
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1413
Size: 464 949
BsiEI CG_ry'CG
Cuts at: 0 892 1413
Size: 892 521
BsiHKAI G_wGCw'C
Cuts at: 0 37 470 1413
Size: 37 433 943
Fragments arranged by size:
943 433 37
BsmI GAATG_Cn'
Cuts at: 0 324 1353 1413
Size: 324 1029 60
Fragments arranged by size:
1029 324 60
Bsp1286I G_dGCh'C
Cuts at: 0 37 470 1012 1080 1413
Size: 37 433 542 68 333
Fragments arranged by size:
542 433 333 68 37
BsrDI GCAATG_nn'
Cuts at: 0 587 954 1065 1213 1413
Size: 587 367 111 148 200
Fragments arranged by size:
587 367 200 148 111
BsrFI r'CCGG_y
Cuts at: 0 416 1242 1413
Size: 416 826 171
Fragments arranged by size:
826 416 171
BstBI TT'CG_AA
Cuts at: 0 655 1413
Size: 655 758
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1413
Size: 510 903
BtgI C'CryG_G
Cuts at: 0 476 1413
Size: 476 937
BtsI GCAGTG_nn'
Cuts at: 0 299 1413
Size: 299 1114
DrdI GACnn_nn'nnGTC
Cuts at: 0 520 936 1161 1413
Size: 520 416 225 252
Fragments arranged by size:
520 416 252 225
EaeI y'GGCC_r
Cuts at: 0 1034 1413
Size: 1034 379
EarI CTCTTCn'nnn_
Cuts at: 0 475 886 1119 1355 1413
Size: 475 411 233 236 58
Fragments arranged by size:
475 411 236 233 58
EcoICRI GAG'CTC
Cuts at: 0 35 1413
Size: 35 1378
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 122 196 546 766 854 1100 1379 1413
Size: 122 74 350 220 88 246 279 34
Fragments arranged by size:
350 279 246 220 122 88 74 34
EcoO109I rG'GnC_Cy
Cuts at: 0 911 1076 1077 1413
Size: 911 165 1 336
Fragments arranged by size:
911 336 165 1
EcoRI G'AATT_C
Cuts at: 0 1290 1413
Size: 1290 123
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 895 927 1092 1124 1413
Size: 82 32 781 32 165 32 289
Fragments arranged by size:
781 289 165 82 32 32 32
HincII GTy'rAC
Cuts at: 0 141 398 1125 1413
Size: 141 257 727 288
Fragments arranged by size:
727 288 257 141
HindIII A'AGCT_T
Cuts at: 0 436 1413
Size: 436 977
HpaI GTT'AAC
Cuts at: 0 398 1413
Size: 398 1015
KpnI G_GTAC'C
Cuts at: 0 417 1413
Size: 417 996
MslI CAynn'nnrTG
Cuts at: 0 189 292 385 748 1413
Size: 189 103 93 363 665
Fragments arranged by size:
665 363 189 103 93
MspA1I CmG'CkG
Cuts at: 0 888 1413
Size: 888 525
NsiI A_TGCA'T
Cuts at: 0 126 1413
Size: 126 1287
NspI r_CATG'y
Cuts at: 0 965 1413
Size: 965 448
PflMI CCAn_nnn'nTGG
Cuts at: 0 637 1413
Size: 637 776
PfoI T'CCnGG_A
Cuts at: 0 212 1413
Size: 212 1201
PpuMI rG'GwC_Cy
Cuts at: 0 911 1413
Size: 911 502
PspOMI G'GGCC_C
Cuts at: 0 1076 1413
Size: 1076 337
PstI C_TGCA'G
Cuts at: 0 317 1413
Size: 317 1096
SacI G_AGCT'C
Cuts at: 0 37 1413
Size: 37 1376
SalI G'TCGA_C
Cuts at: 0 139 1413
Size: 139 1274
SapI GCTCTTCn'nnn_
Cuts at: 0 475 886 1413
Size: 475 411 527
Fragments arranged by size:
527 475 411
SfcI C'TryA_G
Cuts at: 0 81 313 643 1158 1413
Size: 81 232 330 515 255
Fragments arranged by size:
515 330 255 232 81
SpeI A'CTAG_T
Cuts at: 0 1131 1413
Size: 1131 282
SphI G_CATG'C
Cuts at: 0 965 1413
Size: 965 448
TatI w'GTAC_w
Cuts at: 0 1193 1413
Size: 1193 220
Enzymes that do cut:
AccI Acc65I AcuI AgeI AleI ApaI ApoI BanI
BanII BbsI BclI BfrBI Bme1580I BmgBI BpmI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BsrDI
BsrFI BstBI BstXI BtgI BtsI DrdI EaeI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HincII HindIII HpaI
KpnI MslI MspA1I NsiI NspI PflMI PfoI PpuMI
PspOMI PstI SacI SalI SapI SfcI SpeI SphI
TatI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AhdI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BglI BglII
BlpI BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BseRI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII KasI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI PvuII
RsrII SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SrfI SspI StuI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI