Restriction map for
pFN21AB5784
(Linear) (Six-base) MAPSORT of: KIBB5784.seq Check: 3028 from: 1 to: 2310
KIBB5784.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1998 2310
Size: 1998 312
AccI GT'mk_AC
Cuts at: 0 658 982 2310
Size: 658 324 1328
Fragments arranged by size:
1328 658 324
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 1382 1595 2310
Size: 1328 54 213 715
Fragments arranged by size:
1328 715 213 54
AflIII A'CryG_T
Cuts at: 0 407 653 2310
Size: 407 246 1657
Fragments arranged by size:
1657 407 246
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2006 2310
Size: 2006 304
AlwNI CAG_nnn'CTG
Cuts at: 0 1708 1804 2310
Size: 1708 96 506
Fragments arranged by size:
1708 506 96
ApaI G_GGCC'C
Cuts at: 0 1004 1490 1723 2310
Size: 1004 486 233 587
Fragments arranged by size:
1004 587 486 233
ApoI r'AATT_y
Cuts at: 0 741 966 2310
Size: 741 225 1344
Fragments arranged by size:
1344 741 225
BamHI G'GATC_C
Cuts at: 0 2286 2310
Size: 2286 24
BanI G'GyrC_C
Cuts at: 0 1737 2310
Size: 1737 573
BanII G_rGCy'C
Cuts at: 0 1004 1490 1723 2310
Size: 1004 486 233 587
Fragments arranged by size:
1004 587 486 233
BbsI GAAGACnn'nnnn_
Cuts at: 0 1334 1551 2310
Size: 1334 217 759
Fragments arranged by size:
1334 759 217
BbvCI CC'TCA_GC
Cuts at: 0 1859 2310
Size: 1859 451
BfrBI ATG'CAT
Cuts at: 0 2197 2310
Size: 2197 113
BglI GCCn_nnn'nGGC
Cuts at: 0 2248 2310
Size: 2248 62
BglII A'GATC_T
Cuts at: 0 1046 2310
Size: 1046 1264
Bme1580I G_kGCm'C
Cuts at: 0 1004 1490 1723 2000 2310
Size: 1004 486 233 277 310
Fragments arranged by size:
1004 486 310 277 233
BmrI ACTGGGnnnn_n'
Cuts at: 0 335 1474 2310
Size: 335 1139 836
Fragments arranged by size:
1139 836 335
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1685 1881 2310
Size: 932 753 196 429
Fragments arranged by size:
932 753 429 196
Bpu10I CC'TnA_GC
Cuts at: 0 1076 1859 2310
Size: 1076 783 451
Fragments arranged by size:
1076 783 451
BsaI GGTCTCn'nnnn_
Cuts at: 0 257 2310
Size: 257 2053
BsaAI yAC'GTr
Cuts at: 0 408 2310
Size: 408 1902
BsaBI GATnn'nnATC
Cuts at: 0 110 2111 2310
Size: 110 2001 199
Fragments arranged by size:
2001 199 110
BsaHI Gr'CG_yC
Cuts at: 0 1408 2310
Size: 1408 902
BsaWI w'CCGG_w
Cuts at: 0 1055 2310
Size: 1055 1255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1455 1485 1683 1713 2310
Size: 1455 30 198 30 597
Fragments arranged by size:
1455 597 198 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 431 1370 1827 1844 1847 2310
Size: 431 939 457 17 3 463
Fragments arranged by size:
939 463 457 431 17 3
BseYI C'CCAG_C
Cuts at: 0 1112 2310
Size: 1112 1198
BsiHKAI G_wGCw'C
Cuts at: 0 1473 2310
Size: 1473 837
BsmI GAATG_Cn'
Cuts at: 0 2072 2091 2310
Size: 2072 19 219
Fragments arranged by size:
2072 219 19
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1392 2310
Size: 1392 918
Bsp1286I G_dGCh'C
Cuts at: 0 1004 1473 1490 1723 2000 2310
Size: 1004 469 17 233 277 310
Fragments arranged by size:
1004 469 310 277 233 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1998 2310
Size: 1998 312
BsrDI GCAATG_nn'
Cuts at: 0 368 2310
Size: 368 1942
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1744 2310
Size: 1744 566
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1449 2310
Size: 1449 861
BstYI r'GATC_y
Cuts at: 0 275 711 1046 1452 2286 2310
Size: 275 436 335 406 834 24
Fragments arranged by size:
834 436 406 335 275 24
Bsu36I CC'TnA_GG
Cuts at: 0 809 1526 2078 2310
Size: 809 717 552 232
Fragments arranged by size:
809 717 552 232
BtgI C'CryG_G
Cuts at: 0 1087 1537 2310
Size: 1087 450 773
Fragments arranged by size:
1087 773 450
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 149 2310
Size: 149 2161
BtsI GCAGTG_nn'
Cuts at: 0 1127 1910 2310
Size: 1127 783 400
Fragments arranged by size:
1127 783 400
DraI TTT'AAA
Cuts at: 0 2217 2310
Size: 2217 93
DrdI GACnn_nn'nnGTC
Cuts at: 0 1338 2310
Size: 1338 972
EarI CTCTTCn'nnn_
Cuts at: 0 797 1314 1462 2310
Size: 797 517 148 848
Fragments arranged by size:
848 797 517 148
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1328 1382 1595 1685 1881 2310
Size: 932 396 54 213 90 196 429
Fragments arranged by size:
932 429 396 213 196 90 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1208 1529 2310
Size: 1208 321 781
Fragments arranged by size:
1208 781 321
EcoO109I rG'GnC_Cy
Cuts at: 0 91 582 1001 1486 1487 1719 1720 2310
Size: 91 491 419 485 1 232 1 590
Fragments arranged by size:
590 491 485 419 232 91 1 1
EcoRI G'AATT_C
Cuts at: 0 741 966 2310
Size: 741 225 1344
Fragments arranged by size:
1344 741 225
EcoRV GAT'ATC
Cuts at: 0 699 2310
Size: 699 1611
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1260 2310
Size: 1260 1050
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1033 1065 1587 1619 2310
Size: 703 32 298 32 522 32 691
Fragments arranged by size:
703 691 522 298 32 32 32
HincII GTy'rAC
Cuts at: 0 285 630 1020 1606 1754 2310
Size: 285 345 390 586 148 556
Fragments arranged by size:
586 556 390 345 285 148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 1642 1998 2310
Size: 706 936 356 312
Fragments arranged by size:
936 706 356 312
MspA1I CmG'CkG
Cuts at: 0 244 990 1651 2310
Size: 244 746 661 659
Fragments arranged by size:
746 661 659 244
NcoI C'CATG_G
Cuts at: 0 1087 1537 2310
Size: 1087 450 773
Fragments arranged by size:
1087 773 450
NsiI A_TGCA'T
Cuts at: 0 2199 2310
Size: 2199 111
NspI r_CATG'y
Cuts at: 0 657 2310
Size: 657 1653
PciI A'CATG_T
Cuts at: 0 653 2310
Size: 653 1657
PflMI CCAn_nnn'nTGG
Cuts at: 0 1748 2310
Size: 1748 562
PfoI T'CCnGG_A
Cuts at: 0 1584 2310
Size: 1584 726
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 2310
Size: 1150 32 1128
Fragments arranged by size:
1150 1128 32
PspOMI G'GGCC_C
Cuts at: 0 1000 1486 1719 2310
Size: 1000 486 233 591
Fragments arranged by size:
1000 591 486 233
PstI C_TGCA'G
Cuts at: 0 1439 2310
Size: 1439 871
PvuII CAG'CTG
Cuts at: 0 244 1651 2310
Size: 244 1407 659
Fragments arranged by size:
1407 659 244
SfcI C'TryA_G
Cuts at: 0 1427 1435 2310
Size: 1427 8 875
Fragments arranged by size:
1427 875 8
SspI AAT'ATT
Cuts at: 0 1791 2310
Size: 1791 519
StyI C'CwwG_G
Cuts at: 0 1087 1537 1886 2310
Size: 1087 450 349 424
Fragments arranged by size:
1087 450 424 349
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 239 1428 1588 1755 2310
Size: 239 1189 160 167 555
Fragments arranged by size:
1189 555 239 167 160
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 855 887 2022 2054 2310
Size: 855 32 1135 32 256
Fragments arranged by size:
1135 855 256 32 32
XbaI T'CTAG_A
Cuts at: 0 594 2310
Size: 594 1716
XmnI GAAnn'nnTTC
Cuts at: 0 1162 2209 2310
Size: 1162 1047 101
Fragments arranged by size:
1162 1047 101
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApoI
BamHI BanI BanII BbsI BbvCI BfrBI BglI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EarI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI Hin4I HincII MmeI MspA1I NcoI NsiI
NspI PciI PflMI PfoI PpiI PspOMI PstI PvuII
SfcI SspI StyI TaqII TstI XbaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BcgI BciVI BclI BlpI BmgBI BmtI BplI
BpuEI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI CspCI
DraIII EaeI EagI EciI EcoICRI FseI FspI FspAI
HaeII HindIII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NdeI NgoMIV NheI NotI NruI
PacI PasI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TatI
Tth111I XcmI XhoI XmaI ZraI