(Linear) (Six-base) MAPSORT of: KIBB5781.seq Check: 5354 from: 1 to: 2478 KIBB5781.orf With 182 enzymes: SgfI * July 15, 2010 18:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1590 2478 Size: 1590 888 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 439 1694 2478 Size: 439 1255 784 Fragments arranged by size: 1255 784 439 AflIII A'CryG_T Cuts at: 0 29 2478 Size: 29 2449 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1307 2478 Size: 1307 1171 AlwNI CAG_nnn'CTG Cuts at: 0 370 409 2183 2478 Size: 370 39 1774 295 Fragments arranged by size: 1774 370 295 39 ApaI G_GGCC'C Cuts at: 0 83 2478 Size: 83 2395 ApaLI G'TGCA_C Cuts at: 0 1999 2471 2478 Size: 1999 472 7 Fragments arranged by size: 1999 472 7 ApoI r'AATT_y Cuts at: 0 557 1201 1404 1416 1824 2478 Size: 557 644 203 12 408 654 Fragments arranged by size: 654 644 557 408 203 12 AseI AT'TA_AT Cuts at: 0 1340 2478 Size: 1340 1138 AvaI C'yCGr_G Cuts at: 0 176 2478 Size: 176 2302 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 506 539 1297 1330 2478 Size: 506 33 758 33 1148 Fragments arranged by size: 1148 758 506 33 33 BamHI G'GATC_C Cuts at: 0 1188 2478 Size: 1188 1290 BanI G'GyrC_C Cuts at: 0 6 2478 Size: 6 2472 BanII G_rGCy'C Cuts at: 0 76 83 960 2478 Size: 76 7 877 1518 Fragments arranged by size: 1518 877 76 7 BbvCI CC'TCA_GC Cuts at: 0 1749 2478 Size: 1749 729 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 167 201 2478 Size: 167 34 2277 Fragments arranged by size: 2277 167 34 BciVI GTATCCnnnnn_n' Cuts at: 0 1926 2478 Size: 1926 552 BclI T'GATC_A Cuts at: 0 582 1064 1224 2271 2478 Size: 582 482 160 1047 207 Fragments arranged by size: 1047 582 482 207 160 BglII A'GATC_T Cuts at: 0 660 2478 Size: 660 1818 Bme1580I G_kGCm'C Cuts at: 0 83 1697 2003 2475 2478 Size: 83 1614 306 472 3 Fragments arranged by size: 1614 472 306 83 3 BmgBI CAC'GTC Cuts at: 0 107 2154 2478 Size: 107 2047 324 Fragments arranged by size: 2047 324 107 BmrI ACTGGGnnnn_n' Cuts at: 0 276 861 2066 2182 2478 Size: 276 585 1205 116 296 Fragments arranged by size: 1205 585 296 276 116 BmtI G_CTAG'C Cuts at: 0 838 2478 Size: 838 1640 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2115 2478 Size: 2115 363 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 90 260 844 944 1121 1382 2478 Size: 37 53 170 584 100 177 261 1096 Fragments arranged by size: 1096 584 261 177 170 100 53 37 Bpu10I CC'TnA_GC Cuts at: 0 1749 2478 Size: 1749 729 BsaAI yAC'GTr Cuts at: 0 30 2214 2478 Size: 30 2184 264 Fragments arranged by size: 2184 264 30 BsaBI GATnn'nnATC Cuts at: 0 921 1472 2478 Size: 921 551 1006 Fragments arranged by size: 1006 921 551 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1478 1508 1617 1647 2478 Size: 1478 30 109 30 831 Fragments arranged by size: 1478 831 109 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 369 647 1579 2130 2478 Size: 369 278 932 551 348 Fragments arranged by size: 932 551 369 348 278 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1379 1840 2478 Size: 1379 461 638 Fragments arranged by size: 1379 638 461 BsiHKAI G_wGCw'C Cuts at: 0 76 125 1457 2003 2123 2195 2475 2478 Size: 76 49 1332 546 120 72 280 3 Fragments arranged by size: 1332 546 280 120 76 72 49 3 Bsp1286I G_dGCh'C Cuts at: 0 76 83 125 960 1457 1697 2003 2123 Size: 76 7 42 835 497 240 306 120 Cuts at: 2123 2195 2475 2478 Size: 72 280 3 Fragments arranged by size: 835 497 306 280 240 120 76 72 42 7 3 BspHI T'CATG_A Cuts at: 0 843 2478 Size: 843 1635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1590 2478 Size: 1590 888 BsrDI GCAATG_nn' Cuts at: 0 549 1067 1340 2478 Size: 549 518 273 1138 Fragments arranged by size: 1138 549 518 273 BsrFI r'CCGG_y Cuts at: 0 838 2478 Size: 838 1640 BsrGI T'GTAC_A Cuts at: 0 379 2478 Size: 379 2099 BstAPI GCAn_nnn'nTGC Cuts at: 0 110 409 2401 2478 Size: 110 299 1992 77 Fragments arranged by size: 1992 299 110 77 BstXI CCAn_nnnn'nTGG Cuts at: 0 1130 2478 Size: 1130 1348 BstYI r'GATC_y Cuts at: 0 244 366 660 1188 2478 Size: 244 122 294 528 1290 Fragments arranged by size: 1290 528 294 244 122 BtgI C'CryG_G Cuts at: 0 116 932 1083 1249 2478 Size: 116 816 151 166 1229 Fragments arranged by size: 1229 816 166 151 116 BtsI GCAGTG_nn' Cuts at: 0 305 2376 2478 Size: 305 2071 102 Fragments arranged by size: 2071 305 102 DraI TTT'AAA Cuts at: 0 293 464 2478 Size: 293 171 2014 Fragments arranged by size: 2014 293 171 DraIII CAC_nnn'GTG Cuts at: 0 2445 2478 Size: 2445 33 EarI CTCTTCn'nnn_ Cuts at: 0 962 1249 1556 2478 Size: 962 287 307 922 Fragments arranged by size: 962 922 307 287 EcoICRI GAG'CTC Cuts at: 0 74 2478 Size: 74 2404 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 37 90 260 439 844 944 1121 1382 Size: 37 53 170 179 405 100 177 261 Cuts at: 1382 1694 2478 Size: 312 784 Fragments arranged by size: 784 405 312 261 179 177 170 100 53 37 EcoO109I rG'GnC_Cy Cuts at: 0 322 2478 Size: 322 2156 EcoRI G'AATT_C Cuts at: 0 557 2478 Size: 557 1921 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 2067 2265 2478 Size: 2067 198 213 Fragments arranged by size: 2067 213 198 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 351 383 955 987 1056 1088 1478 1510 Size: 351 32 572 32 69 32 390 32 Cuts at: 1510 1924 1956 2478 Size: 414 32 522 Fragments arranged by size: 572 522 414 390 351 69 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 725 1119 1574 2478 Size: 725 394 455 904 Fragments arranged by size: 904 725 455 394 HpaI GTT'AAC Cuts at: 0 1119 1574 2478 Size: 1119 455 904 Fragments arranged by size: 1119 904 455 MfeI C'AATT_G Cuts at: 0 183 2478 Size: 183 2295 MslI CAynn'nnrTG Cuts at: 0 1163 1313 1700 1855 2478 Size: 1163 150 387 155 623 Fragments arranged by size: 1163 623 387 155 150 MspA1I CmG'CkG Cuts at: 0 400 2173 2478 Size: 400 1773 305 Fragments arranged by size: 1773 400 305 NcoI C'CATG_G Cuts at: 0 932 1249 2478 Size: 932 317 1229 Fragments arranged by size: 1229 932 317 NheI G'CTAG_C Cuts at: 0 834 2478 Size: 834 1644 NspI r_CATG'y Cuts at: 0 343 539 1780 2478 Size: 343 196 1241 698 Fragments arranged by size: 1241 698 343 196 PfoI T'CCnGG_A Cuts at: 0 2147 2478 Size: 2147 331 PmlI CAC'GTG Cuts at: 0 30 2478 Size: 30 2448 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 58 90 2478 Size: 58 32 2388 Fragments arranged by size: 2388 58 32 PpuMI rG'GwC_Cy Cuts at: 0 322 2478 Size: 322 2156 PshAI GACnn'nnGTC Cuts at: 0 423 2173 2478 Size: 423 1750 305 Fragments arranged by size: 1750 423 305 PspOMI G'GGCC_C Cuts at: 0 79 2478 Size: 79 2399 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 848 880 2478 Size: 848 32 1598 Fragments arranged by size: 1598 848 32 PstI C_TGCA'G Cuts at: 0 399 405 1658 2478 Size: 399 6 1253 820 Fragments arranged by size: 1253 820 399 6 PvuII CAG'CTG Cuts at: 0 400 2173 2478 Size: 400 1773 305 Fragments arranged by size: 1773 400 305 SacI G_AGCT'C Cuts at: 0 76 2478 Size: 76 2402 ScaI AGT'ACT Cuts at: 0 1581 2478 Size: 1581 897 SexAI A'CCwGG_T Cuts at: 0 1909 2478 Size: 1909 569 SfcI C'TryA_G Cuts at: 0 395 401 901 1654 2478 Size: 395 6 500 753 824 Fragments arranged by size: 824 753 500 395 6 SpeI A'CTAG_T Cuts at: 0 1576 2478 Size: 1576 902 SphI G_CATG'C Cuts at: 0 343 1780 2478 Size: 343 1437 698 Fragments arranged by size: 1437 698 343 SspI AAT'ATT Cuts at: 0 530 1709 2478 Size: 530 1179 769 Fragments arranged by size: 1179 769 530 StuI AGG'CCT Cuts at: 0 1299 2478 Size: 1299 1179 StyI C'CwwG_G Cuts at: 0 932 1249 2478 Size: 932 317 1229 Fragments arranged by size: 1229 932 317 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 2146 2478 Size: 2146 332 TatI w'GTAC_w Cuts at: 0 379 1579 2478 Size: 379 1200 899 Fragments arranged by size: 1200 899 379 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2289 2321 2478 Size: 2289 32 157 Fragments arranged by size: 2289 157 32 Tth111I GACn'n_nGTC Cuts at: 0 1180 2478 Size: 1180 1298 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 90 2478 Size: 90 2388 XmnI GAAnn'nnTTC Cuts at: 0 992 1376 2478 Size: 992 384 1102 Fragments arranged by size: 1102 992 384 Enzymes that do cut: AarI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI AseI AvaI BaeI BamHI BanI BanII BbvCI BcgI BciVI BclI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtsI DraI DraIII EarI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI MslI MspA1I NcoI NheI NspI PfoI PmlI PpiI PpuMI PshAI PspOMI PsrI PstI PvuII SacI ScaI SexAI SfcI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AloI AscI AsiSI AvrII BbeI BbsI BfrBI BglI BlpI BpuEI BsaI BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DrdI EaeI EagI EciI EcoNI EcoRV FseI FspI FspAI HaeII HindIII KasI KpnI MluI MmeI MscI NaeI NarI NdeI NgoMIV NotI NruI NsiI PacI PasI PciI PflMI PmeI PsiI PspXI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI SwaI XbaI XhoI XmaI ZraI