Restriction map for
pFN21AB5778
(Linear) (Six-base) MAPSORT of: KIBB5778.seq Check: 6966 from: 1 to: 2298
KIBB5778.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 2203 2298
Size: 2203 95
AclI AA'CG_TT
Cuts at: 0 2004 2129 2298
Size: 2004 125 169
Fragments arranged by size:
2004 169 125
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 606 1061 1659 2298
Size: 184 422 455 598 639
Fragments arranged by size:
639 598 455 422 184
AflII C'TTAA_G
Cuts at: 0 177 2298
Size: 177 2121
AlwNI CAG_nnn'CTG
Cuts at: 0 781 1669 2298
Size: 781 888 629
Fragments arranged by size:
888 781 629
ApoI r'AATT_y
Cuts at: 0 534 929 1567 2052 2079 2298
Size: 534 395 638 485 27 219
Fragments arranged by size:
638 534 485 395 219 27
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 724 757 2298
Size: 724 33 1541
Fragments arranged by size:
1541 724 33
BanI G'GyrC_C
Cuts at: 0 1198 1959 2298
Size: 1198 761 339
Fragments arranged by size:
1198 761 339
BbeI G_GCGC'C
Cuts at: 0 1963 2298
Size: 1963 335
BbsI GAAGACnn'nnnn_
Cuts at: 0 10 612 1490 1665 1794 2036 2219 2298
Size: 10 602 878 175 129 242 183 79
Fragments arranged by size:
878 602 242 183 175 129 79 10
BciVI GTATCCnnnnn_n'
Cuts at: 0 1977 2298
Size: 1977 321
BfrBI ATG'CAT
Cuts at: 0 1773 2298
Size: 1773 525
BglII A'GATC_T
Cuts at: 0 2153 2298
Size: 2153 145
Bme1580I G_kGCm'C
Cuts at: 0 71 2298
Size: 71 2227
BmrI ACTGGGnnnn_n'
Cuts at: 0 609 1695 1801 2298
Size: 609 1086 106 497
Fragments arranged by size:
1086 609 497 106
BmtI G_CTAG'C
Cuts at: 0 1730 2231 2298
Size: 1730 501 67
Fragments arranged by size:
1730 501 67
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2009 2298
Size: 2009 289
Bpu10I CC'TnA_GC
Cuts at: 0 946 2298
Size: 946 1352
BsaI GGTCTCn'nnnn_
Cuts at: 0 653 2020 2298
Size: 653 1367 278
Fragments arranged by size:
1367 653 278
BsaAI yAC'GTr
Cuts at: 0 360 1902 2298
Size: 360 1542 396
Fragments arranged by size:
1542 396 360
BsaHI Gr'CG_yC
Cuts at: 0 1960 2298
Size: 1960 338
BsaWI w'CCGG_w
Cuts at: 0 1350 1417 2298
Size: 1350 67 881
Fragments arranged by size:
1350 881 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1886 1916 2298
Size: 1886 30 382
Fragments arranged by size:
1886 382 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1583 2298
Size: 1583 715
BseYI C'CCAG_C
Cuts at: 0 33 1055 2298
Size: 33 1022 1243
Fragments arranged by size:
1243 1022 33
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1164 2298
Size: 1164 1134
BsmI GAATG_Cn'
Cuts at: 0 375 1277 2298
Size: 375 902 1021
Fragments arranged by size:
1021 902 375
Bsp1286I G_dGCh'C
Cuts at: 0 71 2298
Size: 71 2227
BspEI T'CCGG_A
Cuts at: 0 1350 2298
Size: 1350 948
BspHI T'CATG_A
Cuts at: 0 195 2063 2298
Size: 195 1868 235
Fragments arranged by size:
1868 235 195
BsrDI GCAATG_nn'
Cuts at: 0 373 1134 2201 2298
Size: 373 761 1067 97
Fragments arranged by size:
1067 761 373 97
BsrGI T'GTAC_A
Cuts at: 0 1994 2298
Size: 1994 304
BstEII G'GTnAC_C
Cuts at: 0 470 1750 2298
Size: 470 1280 548
Fragments arranged by size:
1280 548 470
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1539 2166 2298
Size: 1539 627 132
Fragments arranged by size:
1539 627 132
BstYI r'GATC_y
Cuts at: 0 2153 2298
Size: 2153 145
BstZ17I GTA'TAC
Cuts at: 0 2204 2298
Size: 2204 94
BtgI C'CryG_G
Cuts at: 0 10 71 1955 2217 2298
Size: 10 61 1884 262 81
Fragments arranged by size:
1884 262 81 61 10
BtsI GCAGTG_nn'
Cuts at: 0 733 860 946 2298
Size: 733 127 86 1352
Fragments arranged by size:
1352 733 127 86
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1680 1715 2298
Size: 1680 35 583
Fragments arranged by size:
1680 583 35
DraI TTT'AAA
Cuts at: 0 410 2085 2298
Size: 410 1675 213
Fragments arranged by size:
1675 410 213
DraIII CAC_nnn'GTG
Cuts at: 0 458 2298
Size: 458 1840
DrdI GACnn_nn'nnGTC
Cuts at: 0 694 2298
Size: 694 1604
EarI CTCTTCn'nnn_
Cuts at: 0 428 605 2298
Size: 428 177 1693
Fragments arranged by size:
1693 428 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 606 1061 1659 2009 2298
Size: 184 422 455 598 350 289
Fragments arranged by size:
598 455 422 350 289 184
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 718 950 2053 2298
Size: 718 232 1103 245
Fragments arranged by size:
1103 718 245 232
HaeII r_GCGC'y
Cuts at: 0 1963 2298
Size: 1963 335
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 177 209 287 319 873 905 1209 1241
Size: 177 32 78 32 554 32 304 32
Cuts at: 1241 1575 1607 1751 1783 2298
Size: 334 32 144 32 515
Fragments arranged by size:
554 515 334 304 177 144 78 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1959 2298
Size: 1959 339
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1041 1326 2157 2298
Size: 814 227 285 831 141
Fragments arranged by size:
831 814 285 227 141
MslI CAynn'nnrTG
Cuts at: 0 64 1039 2298
Size: 64 975 1259
Fragments arranged by size:
1259 975 64
MspA1I CmG'CkG
Cuts at: 0 109 423 1407 2298
Size: 109 314 984 891
Fragments arranged by size:
984 891 314 109
NarI GG'CG_CC
Cuts at: 0 1960 2298
Size: 1960 338
NcoI C'CATG_G
Cuts at: 0 2217 2298
Size: 2217 81
NdeI CA'TA_TG
Cuts at: 0 393 495 672 1890 2298
Size: 393 102 177 1218 408
Fragments arranged by size:
1218 408 393 177 102
NheI G'CTAG_C
Cuts at: 0 1726 2227 2298
Size: 1726 501 71
Fragments arranged by size:
1726 501 71
NsiI A_TGCA'T
Cuts at: 0 1775 2298
Size: 1775 523
NspI r_CATG'y
Cuts at: 0 1331 1777 2298
Size: 1331 446 521
Fragments arranged by size:
1331 521 446
PfoI T'CCnGG_A
Cuts at: 0 1478 2298
Size: 1478 820
PsiI TTA'TAA
Cuts at: 0 1295 2298
Size: 1295 1003
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 521 553 2298
Size: 521 32 1745
Fragments arranged by size:
1745 521 32
PstI C_TGCA'G
Cuts at: 0 740 1514 2298
Size: 740 774 784
Fragments arranged by size:
784 774 740
PvuII CAG'CTG
Cuts at: 0 109 423 1407 2298
Size: 109 314 984 891
Fragments arranged by size:
984 891 314 109
ScaI AGT'ACT
Cuts at: 0 1047 1366 1981 2298
Size: 1047 319 615 317
Fragments arranged by size:
1047 615 319 317
SfcI C'TryA_G
Cuts at: 0 104 736 1510 2298
Size: 104 632 774 788
Fragments arranged by size:
788 774 632 104
SfoI GGC'GCC
Cuts at: 0 1961 2298
Size: 1961 337
SmlI C'TyrA_G
Cuts at: 0 177 2298
Size: 177 2121
SnaBI TAC'GTA
Cuts at: 0 1902 2298
Size: 1902 396
SphI G_CATG'C
Cuts at: 0 1777 2298
Size: 1777 521
StuI AGG'CCT
Cuts at: 0 1074 2298
Size: 1074 1224
StyI C'CwwG_G
Cuts at: 0 1755 2217 2298
Size: 1755 462 81
Fragments arranged by size:
1755 462 81
SwaI ATTT'AAAT
Cuts at: 0 410 2298
Size: 410 1888
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 25 957 2298
Size: 25 932 1341
Fragments arranged by size:
1341 932 25
TatI w'GTAC_w
Cuts at: 0 277 1045 1364 1979 1994 2298
Size: 277 768 319 615 15 304
Fragments arranged by size:
768 615 319 304 277 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1919 1940 1951 1972 2298
Size: 1919 21 11 21 326
Fragments arranged by size:
1919 326 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 0 1669 2298
Size: 1669 629
Enzymes that do cut:
AccI AclI AcuI AflII AlwNI ApoI BaeI BanI
BbeI BbsI BciVI BfrBI BglII Bme1580I BmrI BmtI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsmI Bsp1286I BspEI BspHI BsrDI BsrGI
BstEII BstXI BstYI BstZ17I BtgI BtsI CspCI DraI
DraIII DrdI EarI Eco57MI FalI HaeII Hin4I KasI
MmeI MslI MspA1I NarI NcoI NdeI NheI NsiI
NspI PfoI PsiI PsrI PstI PvuII ScaI SfcI
SfoI SmlI SnaBI SphI StuI StyI SwaI TaqII
TatI TstI Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanII BbvCI BcgI BclI BglI BlpI
BmgBI BplI BpuEI BsaBI BsiEI BsiHKAI BsiWI BsmBI
BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI Bsu36I
BtgZI ClaI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI
KpnI MfeI MluI MscI NaeI NgoMIV NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PpuMI
PshAI PspOMI PspXI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SpeI SrfI SspI XbaI XcmI XhoI XmaI XmnI
ZraI