Restriction map for
pFN21AB5773
(Linear) (Six-base) MAPSORT of: KIBB5773.seq Check: 2203 from: 1 to: 2466
KIBB5773.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AatII G_ACGT'C
Cuts at: 0 821 2466
Size: 821 1645
AccI GT'mk_AC
Cuts at: 0 75 2466
Size: 75 2391
Acc65I G'GTAC_C
Cuts at: 0 1379 2466
Size: 1379 1087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 688 1852 2110 2244 2403 2466
Size: 433 255 1164 258 134 159 63
Fragments arranged by size:
1164 433 258 255 159 134 63
AfeI AGC'GCT
Cuts at: 0 1109 2466
Size: 1109 1357
AflII C'TTAA_G
Cuts at: 0 1279 2466
Size: 1279 1187
AflIII A'CryG_T
Cuts at: 0 149 2225 2466
Size: 149 2076 241
Fragments arranged by size:
2076 241 149
AleI CACnn'nnGTG
Cuts at: 0 1756 2466
Size: 1756 710
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 724 1588 2466
Size: 724 864 878
Fragments arranged by size:
878 864 724
AlwNI CAG_nnn'CTG
Cuts at: 0 366 609 642 754 1059 1098 2005 2439
Size: 366 243 33 112 305 39 907 434
Cuts at: 2439 2466
Size: 27
Fragments arranged by size:
907 434 366 305 243 112 39 33
27
ApaI G_GGCC'C
Cuts at: 0 293 1177 1959 2466
Size: 293 884 782 507
Fragments arranged by size:
884 782 507 293
ApaLI G'TGCA_C
Cuts at: 0 1423 2466
Size: 1423 1043
AvaI C'yCGr_G
Cuts at: 0 1040 1395 2114 2466
Size: 1040 355 719 352
Fragments arranged by size:
1040 719 355 352
BamHI G'GATC_C
Cuts at: 0 1826 2466
Size: 1826 640
BanI G'GyrC_C
Cuts at: 0 1379 2466
Size: 1379 1087
BanII G_rGCy'C
Cuts at: 0 293 380 432 887 1013 1177 1187 1959
Size: 293 87 52 455 126 164 10 772
Cuts at: 1959 2466
Size: 507
Fragments arranged by size:
772 507 455 293 164 126 87 52
10
BbsI GAAGACnn'nnnn_
Cuts at: 0 1978 2466
Size: 1978 488
BbvCI CC'TCA_GC
Cuts at: 0 2290 2466
Size: 2290 176
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 316 350 2466
Size: 316 34 2116
Fragments arranged by size:
2116 316 34
BglI GCCn_nnn'nGGC
Cuts at: 0 32 2466
Size: 32 2434
BlpI GC'TnA_GC
Cuts at: 0 304 1412 2466
Size: 304 1108 1054
Fragments arranged by size:
1108 1054 304
Bme1580I G_kGCm'C
Cuts at: 0 293 450 1177 1427 1649 1959 2466
Size: 293 157 727 250 222 310 507
Fragments arranged by size:
727 507 310 293 250 222 157
BmgBI CAC'GTC
Cuts at: 0 1226 2226 2466
Size: 1226 1000 240
Fragments arranged by size:
1226 1000 240
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1801 2466
Size: 1801 665
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 712 871 892 1183 1291 2041 2466
Size: 100 612 159 21 291 108 750 425
Fragments arranged by size:
750 612 425 291 159 108 100 21
Bpu10I CC'TnA_GC
Cuts at: 0 247 295 2290 2466
Size: 247 48 1995 176
Fragments arranged by size:
1995 247 176 48
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1783 2466
Size: 874 909 683
Fragments arranged by size:
909 874 683
BsaI GGTCTCn'nnnn_
Cuts at: 0 2248 2466
Size: 2248 218
BsaAI yAC'GTr
Cuts at: 0 1473 2466
Size: 1473 993
BsaHI Gr'CG_yC
Cuts at: 0 818 1734 2466
Size: 818 916 732
Fragments arranged by size:
916 818 732
BsaWI w'CCGG_w
Cuts at: 0 1013 1344 2466
Size: 1013 331 1122
Fragments arranged by size:
1122 1013 331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 995 1025 1204 1234 2014 2044 2466
Size: 995 30 179 30 780 30 422
Fragments arranged by size:
995 780 422 179 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 871 1039 2202 2466
Size: 664 207 168 1163 264
Fragments arranged by size:
1163 664 264 207 168
BseYI C'CCAG_C
Cuts at: 0 104 561 1059 1136 1598 2387 2413 2466
Size: 104 457 498 77 462 789 26 53
Fragments arranged by size:
789 498 462 457 104 77 53 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 628 730 937 1072 1134 1372 1618
Size: 42 586 102 207 135 62 238 246
Cuts at: 1618 1860 1936 2466
Size: 242 76 530
Fragments arranged by size:
586 530 246 242 238 207 135 102
76 62 42
BsiEI CG_ry'CG
Cuts at: 0 2205 2466
Size: 2205 261
BsiHKAI G_wGCw'C
Cuts at: 0 380 432 887 1013 1169 1427 1597 2466
Size: 380 52 455 126 156 258 170 869
Fragments arranged by size:
869 455 380 258 170 156 126 52
BsmI GAATG_Cn'
Cuts at: 0 1565 2466
Size: 1565 901
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1774 1867 2466
Size: 1774 93 599
Fragments arranged by size:
1774 599 93
Bsp1286I G_dGCh'C
Cuts at: 0 293 380 432 450 887 1013 1169 1177
Size: 293 87 52 18 437 126 156 8
Cuts at: 1177 1187 1427 1597 1649 1959 2466
Size: 10 240 170 52 310 507
Fragments arranged by size:
507 437 310 293 240 170 156 126
87 52 52 18 10 8
BspEI T'CCGG_A
Cuts at: 0 1013 1344 2466
Size: 1013 331 1122
Fragments arranged by size:
1122 1013 331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 1092 2466
Size: 650 442 1374
Fragments arranged by size:
1374 650 442
BsrBI CCG'CTC
Cuts at: 0 1047 2466
Size: 1047 1419
BssSI C'ACGA_G
Cuts at: 0 646 2466
Size: 646 1820
BstAPI GCAn_nnn'nTGC
Cuts at: 0 54 366 642 754 1077 1098 1146 2033
Size: 54 312 276 112 323 21 48 887
Cuts at: 2033 2466
Size: 433
Fragments arranged by size:
887 433 323 312 276 112 54 48
21
BstXI CCAn_nnnn'nTGG
Cuts at: 0 966 2466
Size: 966 1500
BstYI r'GATC_y
Cuts at: 0 276 674 1274 1826 2466
Size: 276 398 600 552 640
Fragments arranged by size:
640 600 552 398 276
Bsu36I CC'TnA_GG
Cuts at: 0 770 2145 2186 2466
Size: 770 1375 41 280
Fragments arranged by size:
1375 770 280 41
BtgI C'CryG_G
Cuts at: 0 36 1383 2466
Size: 36 1347 1083
Fragments arranged by size:
1347 1083 36
BtsI GCAGTG_nn'
Cuts at: 0 361 631 847 1087 2389 2466
Size: 361 270 216 240 1302 77
Fragments arranged by size:
1302 361 270 240 216 77
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1602 1637 2466
Size: 1602 35 829
Fragments arranged by size:
1602 829 35
DraIII CAC_nnn'GTG
Cuts at: 0 829 2466
Size: 829 1637
EaeI y'GGCC_r
Cuts at: 0 725 956 2202 2466
Size: 725 231 1246 264
Fragments arranged by size:
1246 725 264 231
EagI C'GGCC_G
Cuts at: 0 2202 2466
Size: 2202 264
EarI CTCTTCn'nnn_
Cuts at: 0 15 409 1016 1612 2466
Size: 15 394 607 596 854
Fragments arranged by size:
854 607 596 394 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1294 2466
Size: 1294 1172
EcoICRI GAG'CTC
Cuts at: 0 378 430 885 1011 2466
Size: 378 52 455 126 1455
Fragments arranged by size:
1455 455 378 126 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 433 688 712 871 892 1183 1291
Size: 100 333 255 24 159 21 291 108
Cuts at: 1291 1852 2041 2110 2244 2403 2466
Size: 561 189 69 134 159 63
Fragments arranged by size:
561 333 291 255 189 159 159 134
108 100 69 63 24 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 646 709 2466
Size: 646 63 1757
Fragments arranged by size:
1757 646 63
EcoO109I rG'GnC_Cy
Cuts at: 0 42 243 289 290 639 1174 1677 1955
Size: 42 201 46 1 349 535 503 278
Cuts at: 1955 2046 2151 2190 2270 2466
Size: 91 105 39 80 196
Fragments arranged by size:
535 503 349 278 201 196 105 91
80 46 42 39 1
FspI TGC'GCA
Cuts at: 0 520 578 2466
Size: 520 58 1888
Fragments arranged by size:
1888 520 58
HaeII r_GCGC'y
Cuts at: 0 1111 2466
Size: 1111 1355
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 268 300 1204 1236 2466
Size: 268 32 904 32 1230
Fragments arranged by size:
1230 904 268 32 32
KpnI G_GTAC'C
Cuts at: 0 1383 2466
Size: 1383 1083
MscI TGG'CCA
Cuts at: 0 727 958 2466
Size: 727 231 1508
Fragments arranged by size:
1508 727 231
MslI CAynn'nnrTG
Cuts at: 0 964 1756 1903 2466
Size: 964 792 147 563
Fragments arranged by size:
964 792 563 147
MspA1I CmG'CkG
Cuts at: 0 108 366 751 870 891 999 1056 1332
Size: 108 258 385 119 21 108 57 276
Cuts at: 1332 1374 1730 2002 2409 2466
Size: 42 356 272 407 57
Fragments arranged by size:
407 385 356 276 272 258 119 108
108 57 57 42 21
NspI r_CATG'y
Cuts at: 0 153 2466
Size: 153 2313
PasI CC'CwG_GG
Cuts at: 0 30 2466
Size: 30 2436
PciI A'CATG_T
Cuts at: 0 149 2466
Size: 149 2317
PflMI CCAn_nnn'nTGG
Cuts at: 0 1524 2466
Size: 1524 942
PfoI T'CCnGG_A
Cuts at: 0 317 2466
Size: 317 2149
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 955 987 2466
Size: 955 32 1479
Fragments arranged by size:
1479 955 32
PpuMI rG'GwC_Cy
Cuts at: 0 42 639 1677 2046 2466
Size: 42 597 1038 369 420
Fragments arranged by size:
1038 597 420 369 42
PspOMI G'GGCC_C
Cuts at: 0 289 1173 1955 2466
Size: 289 884 782 511
Fragments arranged by size:
884 782 511 289
PstI C_TGCA'G
Cuts at: 0 50 161 371 638 683 750 1094 1103
Size: 50 111 210 267 45 67 344 9
Cuts at: 1103 1133 1154 1208 1337 1985 2444 2466
Size: 30 21 54 129 648 459 22
Fragments arranged by size:
648 459 344 267 210 129 111 67
54 50 45 30 22 21 9
PvuII CAG'CTG
Cuts at: 0 366 751 891 999 1056 1374 2002 2466
Size: 366 385 140 108 57 318 628 464
Fragments arranged by size:
628 464 385 366 318 140 108 57
SacI G_AGCT'C
Cuts at: 0 380 432 887 1013 2466
Size: 380 52 455 126 1453
Fragments arranged by size:
1453 455 380 126 52
SapI GCTCTTCn'nnn_
Cuts at: 0 409 2466
Size: 409 2057
SbfI CC_TGCA'GG
Cuts at: 0 161 683 1208 2466
Size: 161 522 525 1258
Fragments arranged by size:
1258 525 522 161
SexAI A'CCwGG_T
Cuts at: 0 1679 2466
Size: 1679 787
SfcI C'TryA_G
Cuts at: 0 46 157 345 367 634 679 746 1090
Size: 46 111 188 22 267 45 67 344
Cuts at: 1090 1099 1129 1150 1204 1333 1813 1981 2440
Size: 9 30 21 54 129 480 168 459
Cuts at: 2440 2466
Size: 26
Fragments arranged by size:
480 459 344 267 188 168 129 111
67 54 46 45 30 26 22 21
9
SmaI CCC'GGG
Cuts at: 0 1042 2466
Size: 1042 1424
SmlI C'TyrA_G
Cuts at: 0 853 1279 1798 2466
Size: 853 426 519 668
Fragments arranged by size:
853 668 519 426
StyI C'CwwG_G
Cuts at: 0 503 783 1523 1649 2466
Size: 503 280 740 126 817
Fragments arranged by size:
817 740 503 280 126
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1048 1278 1612 1650 1678 1894 2466
Size: 1048 230 334 38 28 216 572
Fragments arranged by size:
1048 572 334 230 216 38 28
TatI w'GTAC_w
Cuts at: 0 1838 2466
Size: 1838 628
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 136 168 803 835 2014 2046 2466
Size: 136 32 635 32 1179 32 420
Fragments arranged by size:
1179 635 420 136 32 32 32
XmaI C'CCGG_G
Cuts at: 0 1040 2466
Size: 1040 1426
XmnI GAAnn'nnTTC
Cuts at: 0 1556 2466
Size: 1556 910
ZraI GAC'GTC
Cuts at: 0 819 2466
Size: 819 1647
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AflII AflIII AleI
AlfI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BglI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI
MscI MslI MspA1I NspI PasI PciI PflMI PfoI
PpiI PpuMI PspOMI PstI PvuII SacI SapI SbfI
SexAI SfcI SmaI SmlI StyI TaqII TatI TstI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AgeI AhdI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbeI BciVI BclI BfrBI BglII
BmrI BmtI BsaBI BsiWI BspHI BsrDI BsrFI BsrGI
BssHII BstBI BstEII BstZ17I BtgZI ClaI DraI DrdI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI ScaI SfiI SfoI SgfI SgrAI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI
XhoI