Restriction map for
pFN21AB5762
(Linear) (Six-base) MAPSORT of: KIBB5762.seq Check: 4511 from: 1 to: 2253
KIBB5762.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 693 1098 2253
Size: 693 405 1155
Fragments arranged by size:
1155 693 405
AccI GT'mk_AC
Cuts at: 0 1373 1553 2253
Size: 1373 180 700
Fragments arranged by size:
1373 700 180
Acc65I G'GTAC_C
Cuts at: 0 398 1856 2253
Size: 398 1458 397
Fragments arranged by size:
1458 398 397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 912 1354 2253
Size: 142 770 442 899
Fragments arranged by size:
899 770 442 142
AfeI AGC'GCT
Cuts at: 0 613 2182 2253
Size: 613 1569 71
Fragments arranged by size:
1569 613 71
AleI CACnn'nnGTG
Cuts at: 0 556 1270 2253
Size: 556 714 983
Fragments arranged by size:
983 714 556
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 964 1516 2253
Size: 964 552 737
Fragments arranged by size:
964 737 552
AlwNI CAG_nnn'CTG
Cuts at: 0 682 1008 1347 1593 1812 2204 2253
Size: 682 326 339 246 219 392 49
Fragments arranged by size:
682 392 339 326 246 219 49
ApaI G_GGCC'C
Cuts at: 0 353 365 2253
Size: 353 12 1888
Fragments arranged by size:
1888 353 12
ApaLI G'TGCA_C
Cuts at: 0 130 1015 1540 2253
Size: 130 885 525 713
Fragments arranged by size:
885 713 525 130
AvaI C'yCGr_G
Cuts at: 0 66 566 1238 1474 1632 2153 2253
Size: 66 500 672 236 158 521 100
Fragments arranged by size:
672 521 500 236 158 100 66
BamHI G'GATC_C
Cuts at: 0 2186 2253
Size: 2186 67
BanI G'GyrC_C
Cuts at: 0 398 1272 1570 1856 2253
Size: 398 874 298 286 397
Fragments arranged by size:
874 398 397 298 286
BanII G_rGCy'C
Cuts at: 0 62 353 365 656 736 1245 1633 2253
Size: 62 291 12 291 80 509 388 620
Fragments arranged by size:
620 509 388 291 291 80 62 12
BbvCI CC'TCA_GC
Cuts at: 0 160 247 2253
Size: 160 87 2006
Fragments arranged by size:
2006 160 87
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1229 1263 2253
Size: 1229 34 990
Fragments arranged by size:
1229 990 34
BfrBI ATG'CAT
Cuts at: 0 1437 2253
Size: 1437 816
BglI GCCn_nnn'nGGC
Cuts at: 0 1281 2253
Size: 1281 972
Bme1580I G_kGCm'C
Cuts at: 0 134 353 365 689 1019 1277 1544 2253
Size: 134 219 12 324 330 258 267 709
Fragments arranged by size:
709 330 324 267 258 219 134 12
BmgBI CAC'GTC
Cuts at: 0 777 2253
Size: 777 1476
BmrI ACTGGGnnnn_n'
Cuts at: 0 653 699 2253
Size: 653 46 1554
Fragments arranged by size:
1554 653 46
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2133 2253
Size: 2133 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 670 808 955 984 1647 1681 2253
Size: 159 511 138 147 29 663 34 572
Fragments arranged by size:
663 572 511 159 147 138 34 29
Bpu10I CC'TnA_GC
Cuts at: 0 106 160 247 2253
Size: 106 54 87 2006
Fragments arranged by size:
2006 106 87 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 1171 2253
Size: 1171 1082
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 391 421 691 721 2253
Size: 391 30 270 30 1532
Fragments arranged by size:
1532 391 270 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 646 837 844 978 981 1846 2253
Size: 439 207 191 7 134 3 865 407
Fragments arranged by size:
865 439 407 207 191 134 7 3
BseYI C'CCAG_C
Cuts at: 0 14 682 874 1124 2253
Size: 14 668 192 250 1129
Fragments arranged by size:
1129 668 250 192 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 1486 1917 2005 2253
Size: 619 867 431 88 248
Fragments arranged by size:
867 619 431 248 88
BsiHKAI G_wGCw'C
Cuts at: 0 134 381 656 676 953 1019 1544 2253
Size: 134 247 275 20 277 66 525 709
Fragments arranged by size:
709 525 277 275 247 134 66 20
Bsp1286I G_dGCh'C
Cuts at: 0 62 134 353 365 381 656 676 689
Size: 62 72 219 12 16 275 20 13
Cuts at: 689 736 953 1019 1245 1277 1544 1633 2253
Size: 47 217 66 226 32 267 89 620
Fragments arranged by size:
620 275 267 226 219 217 89 72
66 62 47 32 20 16 13 12
BspHI T'CATG_A
Cuts at: 0 1146 2253
Size: 1146 1107
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 693 993 1098 1736 2253
Size: 693 300 105 638 517
Fragments arranged by size:
693 638 517 300 105
BsrBI CCG'CTC
Cuts at: 0 893 2253
Size: 893 1360
BsrDI GCAATG_nn'
Cuts at: 0 1034 2253
Size: 1034 1219
BsrFI r'CCGG_y
Cuts at: 0 3 2253
Size: 3 2250
BssSI C'ACGA_G
Cuts at: 0 658 2253
Size: 658 1595
BstAPI GCAn_nnn'nTGC
Cuts at: 0 279 442 1169 2253
Size: 279 163 727 1084
Fragments arranged by size:
1084 727 279 163
BstEII G'GTnAC_C
Cuts at: 0 310 2253
Size: 310 1943
BstXI CCAn_nnnn'nTGG
Cuts at: 0 189 648 1191 1516 2253
Size: 189 459 543 325 737
Fragments arranged by size:
737 543 459 325 189
BstYI r'GATC_y
Cuts at: 0 1787 2186 2253
Size: 1787 399 67
Fragments arranged by size:
1787 399 67
Bsu36I CC'TnA_GG
Cuts at: 0 2192 2253
Size: 2192 61
BtgI C'CryG_G
Cuts at: 0 20 50 188 695 861 915 1488 1796
Size: 20 30 138 507 166 54 573 308
Cuts at: 1796 1940 2072 2253
Size: 144 132 181
Fragments arranged by size:
573 507 308 181 166 144 138 132
54 30 20
BtsI GCAGTG_nn'
Cuts at: 0 134 1070 1591 1874 2253
Size: 134 936 521 283 379
Fragments arranged by size:
936 521 379 283 134
DraI TTT'AAA
Cuts at: 0 1305 2253
Size: 1305 948
DraIII CAC_nnn'GTG
Cuts at: 0 1539 1644 2253
Size: 1539 105 609
Fragments arranged by size:
1539 609 105
DrdI GACnn_nn'nnGTC
Cuts at: 0 781 2253
Size: 781 1472
EaeI y'GGCC_r
Cuts at: 0 47 191 582 1799 2089 2253
Size: 47 144 391 1217 290 164
Fragments arranged by size:
1217 391 290 164 144 47
EarI CTCTTCn'nnn_
Cuts at: 0 847 2253
Size: 847 1406
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 70 1040 2253
Size: 70 970 1213
Fragments arranged by size:
1213 970 70
EcoICRI GAG'CTC
Cuts at: 0 654 2253
Size: 654 1599
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 159 670 808 912 955 984 1354
Size: 142 17 511 138 104 43 29 370
Cuts at: 1354 1647 1681 2253
Size: 293 34 572
Fragments arranged by size:
572 511 370 293 142 138 104 43
34 29 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1851 2253
Size: 1851 402
EcoO109I rG'GnC_Cy
Cuts at: 0 349 350 361 362 792 1005 1128 1932
Size: 349 1 11 1 430 213 123 804
Cuts at: 1932 1989 2112 2237 2253
Size: 57 123 125 16
Fragments arranged by size:
804 430 349 213 125 123 123 57
16 11 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1599 2253
Size: 1599 654
HaeII r_GCGC'y
Cuts at: 0 591 615 1072 1689 2184 2253
Size: 591 24 457 617 495 69
Fragments arranged by size:
617 591 495 457 69 24
KpnI G_GTAC'C
Cuts at: 0 402 1860 2253
Size: 402 1458 393
Fragments arranged by size:
1458 402 393
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1322 2253
Size: 1322 931
MscI TGG'CCA
Cuts at: 0 193 584 2253
Size: 193 391 1669
Fragments arranged by size:
1669 391 193
MslI CAynn'nnrTG
Cuts at: 0 34 187 217 326 556 886 1208 1270
Size: 34 153 30 109 230 330 322 62
Cuts at: 1270 2253
Size: 983
Fragments arranged by size:
983 330 322 230 153 109 62 34
30
MspA1I CmG'CkG
Cuts at: 0 798 863 2253
Size: 798 65 1390
Fragments arranged by size:
1390 798 65
NaeI GCC'GGC
Cuts at: 0 5 2253
Size: 5 2248
NcoI C'CATG_G
Cuts at: 0 50 188 1796 2253
Size: 50 138 1608 457
Fragments arranged by size:
1608 457 138 50
NgoMIV G'CCGG_C
Cuts at: 0 3 2253
Size: 3 2250
NsiI A_TGCA'T
Cuts at: 0 1439 2253
Size: 1439 814
NspI r_CATG'y
Cuts at: 0 375 1101 1441 2253
Size: 375 726 340 812
Fragments arranged by size:
812 726 375 340
PasI CC'CwG_GG
Cuts at: 0 9 1757 1928 2253
Size: 9 1748 171 325
Fragments arranged by size:
1748 325 171 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 1625 1797 1847 2253
Size: 1347 278 172 50 406
Fragments arranged by size:
1347 406 278 172 50
PfoI T'CCnGG_A
Cuts at: 0 785 1190 2253
Size: 785 405 1063
Fragments arranged by size:
1063 785 405
PpuMI rG'GwC_Cy
Cuts at: 0 792 1005 1932 1989 2112 2237 2253
Size: 792 213 927 57 123 125 16
Fragments arranged by size:
927 792 213 125 123 57 16
PspOMI G'GGCC_C
Cuts at: 0 349 361 2253
Size: 349 12 1892
Fragments arranged by size:
1892 349 12
PstI C_TGCA'G
Cuts at: 0 44 170 467 1004 1095 1598 1787 2253
Size: 44 126 297 537 91 503 189 466
Fragments arranged by size:
537 503 466 297 189 126 91 44
SacI G_AGCT'C
Cuts at: 0 656 2253
Size: 656 1597
SacII CC_GC'GG
Cuts at: 0 864 2253
Size: 864 1389
SanDI GG'GwC_CC
Cuts at: 0 1932 2253
Size: 1932 321
SbfI CC_TGCA'GG
Cuts at: 0 467 1095 2253
Size: 467 628 1158
Fragments arranged by size:
1158 628 467
ScaI AGT'ACT
Cuts at: 0 1675 2253
Size: 1675 578
SexAI A'CCwGG_T
Cuts at: 0 1805 1991 2253
Size: 1805 186 262
Fragments arranged by size:
1805 262 186
SfcI C'TryA_G
Cuts at: 0 40 166 463 1000 1091 1594 1783 2241
Size: 40 126 297 537 91 503 189 458
Cuts at: 2241 2253
Size: 12
Fragments arranged by size:
537 503 458 297 189 126 91 40
12
SmaI CCC'GGG
Cuts at: 0 68 568 2253
Size: 68 500 1685
Fragments arranged by size:
1685 500 68
SphI G_CATG'C
Cuts at: 0 1101 1441 2253
Size: 1101 340 812
Fragments arranged by size:
1101 812 340
StuI AGG'CCT
Cuts at: 0 393 2253
Size: 393 1860
StyI C'CwwG_G
Cuts at: 0 50 188 1796 2253
Size: 50 138 1608 457
Fragments arranged by size:
1608 457 138 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1522 1992 2253
Size: 1522 470 261
Fragments arranged by size:
1522 470 261
TatI w'GTAC_w
Cuts at: 0 1673 2253
Size: 1673 580
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 691 723 1279 1311 2253
Size: 691 32 556 32 942
Fragments arranged by size:
942 691 556 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1225 2253
Size: 1225 1028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1419 2253
Size: 1419 834
XmaI C'CCGG_G
Cuts at: 0 66 566 2253
Size: 66 500 1687
Fragments arranged by size:
1687 500 66
XmnI GAAnn'nnTTC
Cuts at: 0 1582 2253
Size: 1582 671
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbvCI BcgI
BfrBI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BsaI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
KpnI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PasI PflMI PfoI PpuMI PspOMI PstI
SacI SacII SanDI SbfI ScaI SexAI SfcI SmaI
SphI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AflIII AgeI AhdI AloI ApoI
AscI AseI AsiSI AvrII BaeI BbeI BbsI BciVI
BclI BglII BlpI BmtI BpuEI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrGI BssHII
BstBI BstZ17I BtgZI ClaI CspCI EagI EcoRI EcoRV
FseI FspI FspAI Hin4I HincII HindIII HpaI KasI
MfeI MluI NarI NdeI NheI NotI NruI PacI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SalI SapI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SrfI SspI SwaI XbaI
XhoI ZraI