Restriction map for
pFN21AB5757
(Linear) (Six-base) MAPSORT of: KIBB5757.seq Check: 5983 from: 1 to: 2442
KIBB5757.orf
With 182 enzymes: SgfI *
December 5, 2014 12:53 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 681 2442
Size: 681 1761
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 588 2442
Size: 336 252 1854
Fragments arranged by size:
1854 336 252
AfeI AGC'GCT
Cuts at: 0 563 1781 2030 2442
Size: 563 1218 249 412
Fragments arranged by size:
1218 563 412 249
AflIII A'CryG_T
Cuts at: 0 701 2333 2442
Size: 701 1632 109
Fragments arranged by size:
1632 701 109
AhdI GACnn_n'nnGTC
Cuts at: 0 1645 2442
Size: 1645 797
AleI CACnn'nnGTG
Cuts at: 0 508 1177 2442
Size: 508 669 1265
Fragments arranged by size:
1265 669 508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1067 1930 2442
Size: 1067 863 512
Fragments arranged by size:
1067 863 512
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 2442
Size: 27 2415
AlwNI CAG_nnn'CTG
Cuts at: 0 222 727 1282 1722 2303 2442
Size: 222 505 555 440 581 139
Fragments arranged by size:
581 555 505 440 222 139
ApaI G_GGCC'C
Cuts at: 0 1545 2312 2442
Size: 1545 767 130
Fragments arranged by size:
1545 767 130
AvaI C'yCGr_G
Cuts at: 0 25 272 544 595 772 1196 1689 1901
Size: 25 247 272 51 177 424 493 212
Cuts at: 1901 2102 2442
Size: 201 340
Fragments arranged by size:
493 424 340 272 247 212 201 177
51 25
BamHI G'GATC_C
Cuts at: 0 385 1289 2442
Size: 385 904 1153
Fragments arranged by size:
1153 904 385
BanI G'GyrC_C
Cuts at: 0 101 108 502 1161 1399 1510 2342 2442
Size: 101 7 394 659 238 111 832 100
Fragments arranged by size:
832 659 394 238 111 101 100 7
BanII G_rGCy'C
Cuts at: 0 272 602 620 1115 1545 1567 1583 2251
Size: 272 330 18 495 430 22 16 668
Cuts at: 2251 2312 2442
Size: 61 130
Fragments arranged by size:
668 495 430 330 272 130 61 22
18 16
BbeI G_GCGC'C
Cuts at: 0 105 112 1514 2442
Size: 105 7 1402 928
Fragments arranged by size:
1402 928 105 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 273 303 346 1262 2442
Size: 273 30 43 916 1180
Fragments arranged by size:
1180 916 273 43 30
BbvCI CC'TCA_GC
Cuts at: 0 732 1600 1669 1804 2442
Size: 732 868 69 135 638
Fragments arranged by size:
868 732 638 135 69
BciVI GTATCCnnnnn_n'
Cuts at: 0 171 2442
Size: 171 2271
BglI GCCn_nnn'nGGC
Cuts at: 0 811 1060 1745 2326 2442
Size: 811 249 685 581 116
Fragments arranged by size:
811 685 581 249 116
BlpI GC'TnA_GC
Cuts at: 0 1064 1228 1309 1561 2032 2245 2442
Size: 1064 164 81 252 471 213 197
Fragments arranged by size:
1064 471 252 213 197 164 81
Bme1580I G_kGCm'C
Cuts at: 0 1164 1545 1798 2312 2345 2412 2442
Size: 1164 381 253 514 33 67 30
Fragments arranged by size:
1164 514 381 253 67 33 30
BmrI ACTGGGnnnn_n'
Cuts at: 0 888 2171 2313 2442
Size: 888 1283 142 129
Fragments arranged by size:
1283 888 142 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 733 2057 2442
Size: 733 1324 385
Fragments arranged by size:
1324 733 385
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 415 433 505 728 1441 1614 2442
Size: 415 18 72 223 713 173 828
Fragments arranged by size:
828 713 415 223 173 72 18
Bpu10I CC'TnA_GC
Cuts at: 0 118 732 1600 1669 1804 2442
Size: 118 614 868 69 135 638
Fragments arranged by size:
868 638 614 135 118 69
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1315 2442
Size: 1315 1127
BsaAI yAC'GTr
Cuts at: 0 1305 2442
Size: 1305 1137
BsaHI Gr'CG_yC
Cuts at: 0 5 102 109 278 837 1318 1373 1511
Size: 5 97 7 169 559 481 55 138
Cuts at: 1511 1757 1994 2442
Size: 246 237 448
Fragments arranged by size:
559 481 448 246 237 169 138 97
55 7 5
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 815 845 2442
Size: 815 30 1597
Fragments arranged by size:
1597 815 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 7 90 280 1492 1996 2442
Size: 7 83 190 1212 504 446
Fragments arranged by size:
1212 504 446 190 83 7
BseYI C'CCAG_C
Cuts at: 0 222 1298 1379 1703 2093 2442
Size: 222 1076 81 324 390 349
Fragments arranged by size:
1076 390 349 324 222 81
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1045 1117 1494 1645 1903 2071 2442
Size: 512 533 72 377 151 258 168 371
Fragments arranged by size:
533 512 377 371 258 168 151 72
BsiEI CG_ry'CG
Cuts at: 0 1587 1636 2442
Size: 1587 49 806
Fragments arranged by size:
1587 806 49
BsiHKAI G_wGCw'C
Cuts at: 0 602 620 1310 1583 2251 2442
Size: 602 18 690 273 668 191
Fragments arranged by size:
690 668 602 273 191 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 184 541 704 2442
Size: 184 357 163 1738
Fragments arranged by size:
1738 357 184 163
Bsp1286I G_dGCh'C
Cuts at: 0 272 602 620 1115 1164 1310 1545 1567
Size: 272 330 18 495 49 146 235 22
Cuts at: 1567 1583 1798 2251 2312 2345 2412 2442
Size: 16 215 453 61 33 67 30
Fragments arranged by size:
495 453 330 272 235 215 146 67
61 49 33 30 22 18 16
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 681 869 1535 2442
Size: 681 188 666 907
Fragments arranged by size:
907 681 666 188
BsrBI CCG'CTC
Cuts at: 0 1068 2442
Size: 1068 1374
BsrFI r'CCGG_y
Cuts at: 0 443 1396 2442
Size: 443 953 1046
Fragments arranged by size:
1046 953 443
BsrGI T'GTAC_A
Cuts at: 0 674 2442
Size: 674 1768
BssHII G'CGCG_C
Cuts at: 0 1624 2442
Size: 1624 818
BssSI C'ACGA_G
Cuts at: 0 1867 2442
Size: 1867 575
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1472 2442
Size: 1472 970
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 1359 2124 2442
Size: 411 948 765 318
Fragments arranged by size:
948 765 411 318
BstYI r'GATC_y
Cuts at: 0 385 629 1289 1415 2442
Size: 385 244 660 126 1027
Fragments arranged by size:
1027 660 385 244 126
Bsu36I CC'TnA_GG
Cuts at: 0 1057 2442
Size: 1057 1385
BtgI C'CryG_G
Cuts at: 0 404 1514 1536 2442
Size: 404 1110 22 906
Fragments arranged by size:
1110 906 404 22
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2115 2285 2442
Size: 2115 170 157
Fragments arranged by size:
2115 170 157
BtsI GCAGTG_nn'
Cuts at: 0 725 2410 2442
Size: 725 1685 32
Fragments arranged by size:
1685 725 32
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 863 898 2442
Size: 863 35 1544
Fragments arranged by size:
1544 863 35
EaeI y'GGCC_r
Cuts at: 0 445 474 884 1181 1284 1584 1633 1832
Size: 445 29 410 297 103 300 49 199
Cuts at: 1832 2442
Size: 610
Fragments arranged by size:
610 445 410 300 297 199 103 49
29
EagI C'GGCC_G
Cuts at: 0 1584 1633 2442
Size: 1584 49 809
Fragments arranged by size:
1584 809 49
EarI CTCTTCn'nnn_
Cuts at: 0 607 2442
Size: 607 1835
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 712 2442
Size: 712 1730
EcoICRI GAG'CTC
Cuts at: 0 600 618 1581 2249 2442
Size: 600 18 963 668 193
Fragments arranged by size:
963 668 600 193 18
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 336 415 433 505 588 728 1441 1614
Size: 336 79 18 72 83 140 713 173
Cuts at: 1614 2442
Size: 828
Fragments arranged by size:
828 713 336 173 140 83 79 72
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 460 583 1153 1363 1603 2442
Size: 460 123 570 210 240 839
Fragments arranged by size:
839 570 460 240 210 123
EcoO109I rG'GnC_Cy
Cuts at: 0 134 250 399 858 978 1035 1200 1524
Size: 134 116 149 459 120 57 165 324
Cuts at: 1524 1541 1542 1647 2308 2309 2442
Size: 17 1 105 661 1 133
Fragments arranged by size:
661 459 324 165 149 134 133 120
116 105 57 17 1 1
HaeII r_GCGC'y
Cuts at: 0 105 112 565 1514 1783 1841 2032 2442
Size: 105 7 453 949 269 58 191 410
Fragments arranged by size:
949 453 410 269 191 105 58 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1340 1360 1372 1392 1631 1663 1934 1966
Size: 1340 20 12 20 239 32 271 32
Cuts at: 1966 2442
Size: 476
Fragments arranged by size:
1340 476 271 239 32 32 20 20
12
HincII GTy'rAC
Cuts at: 0 1611 2442
Size: 1611 831
KasI G'GCGC_C
Cuts at: 0 101 108 1510 2442
Size: 101 7 1402 932
Fragments arranged by size:
1402 932 101 7
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 2336 2442
Size: 188 2148 106
Fragments arranged by size:
2148 188 106
MscI TGG'CCA
Cuts at: 0 886 1183 1834 2442
Size: 886 297 651 608
Fragments arranged by size:
886 651 608 297
MslI CAynn'nnrTG
Cuts at: 0 508 1177 2442
Size: 508 669 1265
Fragments arranged by size:
1265 669 508
MspA1I CmG'CkG
Cuts at: 0 222 821 1041 1218 1560 1742 2013 2442
Size: 222 599 220 177 342 182 271 429
Fragments arranged by size:
599 429 342 271 222 220 182 177
NarI GG'CG_CC
Cuts at: 0 102 109 1511 2442
Size: 102 7 1402 931
Fragments arranged by size:
1402 931 102 7
NotI GC'GGCC_GC
Cuts at: 0 1633 2442
Size: 1633 809
NspI r_CATG'y
Cuts at: 0 705 1096 2337 2442
Size: 705 391 1241 105
Fragments arranged by size:
1241 705 391 105
PciI A'CATG_T
Cuts at: 0 701 2333 2442
Size: 701 1632 109
Fragments arranged by size:
1632 701 109
PfoI T'CCnGG_A
Cuts at: 0 66 899 1124 1418 1682 2442
Size: 66 833 225 294 264 760
Fragments arranged by size:
833 760 294 264 225 66
PmlI CAC'GTG
Cuts at: 0 1305 2442
Size: 1305 1137
PpuMI rG'GwC_Cy
Cuts at: 0 250 399 858 1200 1524 1647 2442
Size: 250 149 459 342 324 123 795
Fragments arranged by size:
795 459 342 324 250 149 123
PspOMI G'GGCC_C
Cuts at: 0 1541 2308 2442
Size: 1541 767 134
Fragments arranged by size:
1541 767 134
PstI C_TGCA'G
Cuts at: 0 461 584 692 1046 1223 2166 2442
Size: 461 123 108 354 177 943 276
Fragments arranged by size:
943 461 354 276 177 123 108
PvuII CAG'CTG
Cuts at: 0 222 1218 1560 2013 2442
Size: 222 996 342 453 429
Fragments arranged by size:
996 453 429 342 222
SacI G_AGCT'C
Cuts at: 0 602 620 1583 2251 2442
Size: 602 18 963 668 191
Fragments arranged by size:
963 668 602 191 18
SanDI GG'GwC_CC
Cuts at: 0 250 1200 2442
Size: 250 950 1242
Fragments arranged by size:
1242 950 250
SapI GCTCTTCn'nnn_
Cuts at: 0 607 2442
Size: 607 1835
SbfI CC_TGCA'GG
Cuts at: 0 461 692 2442
Size: 461 231 1750
Fragments arranged by size:
1750 461 231
SexAI A'CCwGG_T
Cuts at: 0 1643 2442
Size: 1643 799
SfcI C'TryA_G
Cuts at: 0 237 457 580 688 1042 1219 2162 2442
Size: 237 220 123 108 354 177 943 280
Fragments arranged by size:
943 354 280 237 220 177 123 108
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 811 2442
Size: 811 1631
SfoI GGC'GCC
Cuts at: 0 103 110 1512 2442
Size: 103 7 1402 930
Fragments arranged by size:
1402 930 103 7
SmaI CCC'GGG
Cuts at: 0 274 1198 2442
Size: 274 924 1244
Fragments arranged by size:
1244 924 274
SmlI C'TyrA_G
Cuts at: 0 1330 2442
Size: 1330 1112
StuI AGG'CCT
Cuts at: 0 1499 1658 1802 2442
Size: 1499 159 144 640
Fragments arranged by size:
1499 640 159 144
StyI C'CwwG_G
Cuts at: 0 1079 1238 1488 2442
Size: 1079 159 250 954
Fragments arranged by size:
1079 954 250 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 870 2442
Size: 870 1572
TatI w'GTAC_w
Cuts at: 0 674 2442
Size: 674 1768
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 242 274 815 847 2442
Size: 242 32 541 32 1595
Fragments arranged by size:
1595 541 242 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1756 1948 2442
Size: 1756 192 494
Fragments arranged by size:
1756 494 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1416 2121 2442
Size: 1416 705 321
Fragments arranged by size:
1416 705 321
XmaI C'CCGG_G
Cuts at: 0 272 1196 2442
Size: 272 924 1246
Fragments arranged by size:
1246 924 272
XmnI GAAnn'nnTTC
Cuts at: 0 310 2442
Size: 310 2132
Enzymes that do cut:
AarI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApaI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BciVI BglI BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NotI NspI PciI PfoI PmlI
PpuMI PspOMI PstI PvuII SacI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI
BglII BmgBI BmtI BsaI BsaBI BsaWI BsiWI BsmI
BspEI BspHI BsrDI BstBI BstEII BstZ17I ClaI DraI
DraIII DrdI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NruI NsiI PacI PasI PflMI PmeI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacII
SalI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI XbaI XhoI ZraI