Restriction map for
pFN21AB5755
(Linear) (Six-base) MAPSORT of: KIBB5755.seq Check: 2465 from: 1 to: 2430
KIBB5755.orf
With 182 enzymes: SgfI *
May 6, 2016 19:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 187 423 2430
Size: 187 236 2007
Fragments arranged by size:
2007 236 187
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 847 2430
Size: 706 141 1583
Fragments arranged by size:
1583 706 141
AfeI AGC'GCT
Cuts at: 0 757 2430
Size: 757 1673
AleI CACnn'nnGTG
Cuts at: 0 896 1817 2430
Size: 896 921 613
Fragments arranged by size:
921 896 613
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 914 2430
Size: 914 1516
AlwNI CAG_nnn'CTG
Cuts at: 0 825 2430
Size: 825 1605
ApaI G_GGCC'C
Cuts at: 0 485 996 2430
Size: 485 511 1434
Fragments arranged by size:
1434 511 485
ApaLI G'TGCA_C
Cuts at: 0 899 1913 2351 2430
Size: 899 1014 438 79
Fragments arranged by size:
1014 899 438 79
ApoI r'AATT_y
Cuts at: 0 172 587 667 951 2430
Size: 172 415 80 284 1479
Fragments arranged by size:
1479 415 284 172 80
BanI G'GyrC_C
Cuts at: 0 1001 1223 1825 2430
Size: 1001 222 602 605
Fragments arranged by size:
1001 605 602 222
BanII G_rGCy'C
Cuts at: 0 485 640 996 1529 1982 2430
Size: 485 155 356 533 453 448
Fragments arranged by size:
533 485 453 448 356 155
BbsI GAAGACnn'nnnn_
Cuts at: 0 1237 1442 2430
Size: 1237 205 988
Fragments arranged by size:
1237 988 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2215 2249 2430
Size: 2215 34 181
Fragments arranged by size:
2215 181 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 646 1080 1456 2430
Size: 646 434 376 974
Fragments arranged by size:
974 646 434 376
BfrBI ATG'CAT
Cuts at: 0 558 2430
Size: 558 1872
BlpI GC'TnA_GC
Cuts at: 0 2011 2430
Size: 2011 419
Bme1580I G_kGCm'C
Cuts at: 0 485 881 903 996 1917 2355 2430
Size: 485 396 22 93 921 438 75
Fragments arranged by size:
921 485 438 396 93 75 22
BmrI ACTGGGnnnn_n'
Cuts at: 0 323 1060 1502 1521 2430
Size: 323 737 442 19 909
Fragments arranged by size:
909 737 442 323 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 915 1535 2430
Size: 460 455 620 895
Fragments arranged by size:
895 620 460 455
Bpu10I CC'TnA_GC
Cuts at: 0 1103 1362 2430
Size: 1103 259 1068
Fragments arranged by size:
1103 1068 259
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 1694 2131 2430
Size: 556 1138 437 299
Fragments arranged by size:
1138 556 437 299
BsaHI Gr'CG_yC
Cuts at: 0 1498 2430
Size: 1498 932
BsaWI w'CCGG_w
Cuts at: 0 2084 2430
Size: 2084 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 777 807 2430
Size: 777 30 1623
Fragments arranged by size:
1623 777 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 175 457 1050 2430
Size: 175 282 593 1380
Fragments arranged by size:
1380 593 282 175
BseYI C'CCAG_C
Cuts at: 0 120 758 1324 2046 2108 2156 2236 2430
Size: 120 638 566 722 62 48 80 194
Fragments arranged by size:
722 638 566 194 120 80 62 48
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 167 768 2430
Size: 167 601 1662
Fragments arranged by size:
1662 601 167
BsiHKAI G_wGCw'C
Cuts at: 0 903 1917 2355 2430
Size: 903 1014 438 75
Fragments arranged by size:
1014 903 438 75
BsmI GAATG_Cn'
Cuts at: 0 187 619 1814 2430
Size: 187 432 1195 616
Fragments arranged by size:
1195 616 432 187
Bsp1286I G_dGCh'C
Cuts at: 0 485 640 881 903 996 1529 1917 1982
Size: 485 155 241 22 93 533 388 65
Cuts at: 1982 2355 2430
Size: 373 75
Fragments arranged by size:
533 485 388 373 241 155 93 75
65 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 187 423 2430
Size: 187 236 2007
Fragments arranged by size:
2007 236 187
BsrDI GCAATG_nn'
Cuts at: 0 558 2430
Size: 558 1872
BsrFI r'CCGG_y
Cuts at: 0 1220 2430
Size: 1220 1210
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1141 1772 2430
Size: 1141 631 658
Fragments arranged by size:
1141 658 631
BtgI C'CryG_G
Cuts at: 0 997 1073 1134 1564 2430
Size: 997 76 61 430 866
Fragments arranged by size:
997 866 430 76 61
BtsI GCAGTG_nn'
Cuts at: 0 570 1863 2430
Size: 570 1293 567
Fragments arranged by size:
1293 570 567
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1009 1044 2054 2089 2430
Size: 1009 35 1010 35 341
Fragments arranged by size:
1010 1009 341 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1327 2430
Size: 1327 1103
EaeI y'GGCC_r
Cuts at: 0 2246 2430
Size: 2246 184
EarI CTCTTCn'nnn_
Cuts at: 0 539 2205 2430
Size: 539 1666 225
Fragments arranged by size:
1666 539 225
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1382 2430
Size: 1382 1048
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 706 847 915 1535 2430
Size: 460 246 141 68 620 895
Fragments arranged by size:
895 620 460 246 141 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2146 2430
Size: 2146 284
EcoO109I rG'GnC_Cy
Cuts at: 0 481 482 992 993 1300 2284 2430
Size: 481 1 510 1 307 984 146
Fragments arranged by size:
984 510 481 307 146 1 1
EcoRI G'AATT_C
Cuts at: 0 172 2430
Size: 172 2258
EcoRV GAT'ATC
Cuts at: 0 1835 2430
Size: 1835 595
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 37 1807 2430
Size: 37 1770 623
Fragments arranged by size:
1770 623 37
HaeII r_GCGC'y
Cuts at: 0 759 2430
Size: 759 1671
HincII GTy'rAC
Cuts at: 0 1853 2430
Size: 1853 577
MfeI C'AATT_G
Cuts at: 0 1081 2430
Size: 1081 1349
MslI CAynn'nnrTG
Cuts at: 0 259 563 647 896 1139 1817 2430
Size: 259 304 84 249 243 678 613
Fragments arranged by size:
678 613 304 259 249 243 84
MspA1I CmG'CkG
Cuts at: 0 120 782 2430
Size: 120 662 1648
Fragments arranged by size:
1648 662 120
NcoI C'CATG_G
Cuts at: 0 997 1134 2430
Size: 997 137 1296
Fragments arranged by size:
1296 997 137
NsiI A_TGCA'T
Cuts at: 0 560 2430
Size: 560 1870
NspI r_CATG'y
Cuts at: 0 1945 2430
Size: 1945 485
PasI CC'CwG_GG
Cuts at: 0 84 2430
Size: 84 2346
PflMI CCAn_nnn'nTGG
Cuts at: 0 805 1255 1869 2430
Size: 805 450 614 561
Fragments arranged by size:
805 614 561 450
PfoI T'CCnGG_A
Cuts at: 0 1121 1731 2430
Size: 1121 610 699
Fragments arranged by size:
1121 699 610
PpuMI rG'GwC_Cy
Cuts at: 0 2284 2430
Size: 2284 146
PspOMI G'GGCC_C
Cuts at: 0 481 992 2430
Size: 481 511 1438
Fragments arranged by size:
1438 511 481
PstI C_TGCA'G
Cuts at: 0 184 457 1279 2430
Size: 184 273 822 1151
Fragments arranged by size:
1151 822 273 184
PvuII CAG'CTG
Cuts at: 0 120 2430
Size: 120 2310
RsrII CG'GwC_CG
Cuts at: 0 2087 2430
Size: 2087 343
SbfI CC_TGCA'GG
Cuts at: 0 184 2430
Size: 184 2246
ScaI AGT'ACT
Cuts at: 0 1036 1342 2430
Size: 1036 306 1088
Fragments arranged by size:
1088 1036 306
SfcI C'TryA_G
Cuts at: 0 180 330 453 1275 2430
Size: 180 150 123 822 1155
Fragments arranged by size:
1155 822 180 150 123
SmlI C'TyrA_G
Cuts at: 0 535 1709 2146 2430
Size: 535 1174 437 284
Fragments arranged by size:
1174 535 437 284
StuI AGG'CCT
Cuts at: 0 622 1887 2430
Size: 622 1265 543
Fragments arranged by size:
1265 622 543
StyI C'CwwG_G
Cuts at: 0 727 997 1134 1882 2121 2430
Size: 727 270 137 748 239 309
Fragments arranged by size:
748 727 309 270 239 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1778 1836 1900 2104 2430
Size: 1778 58 64 204 326
Fragments arranged by size:
1778 326 204 64 58
TatI w'GTAC_w
Cuts at: 0 1034 1340 2430
Size: 1034 306 1090
Fragments arranged by size:
1090 1034 306
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 777 809 1623 1655 1753 1785 2430
Size: 777 32 814 32 98 32 645
Fragments arranged by size:
814 777 645 98 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 342 600 2430
Size: 342 258 1830
Fragments arranged by size:
1830 342 258
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 606 1080 1508 2128 2275 2430
Size: 606 474 428 620 147 155
Fragments arranged by size:
620 606 474 428 155 147
XmnI GAAnn'nnTTC
Cuts at: 0 17 2430
Size: 17 2413
Enzymes that do cut:
AarI AcuI AfeI AleI AlfI AlwNI ApaI ApaLI
ApoI BanI BanII BbsI BcgI BciVI BfrBI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BsrFI BstXI BtgI BtsI CspCI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
HincII MfeI MslI MspA1I NcoI NsiI NspI PasI
PflMI PfoI PpuMI PspOMI PstI PvuII RsrII SbfI
ScaI SfcI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AloI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BclI BglI BglII BmgBI BmtI BplI
BsaI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EcoICRI
FseI FspI FspAI Hin4I HindIII HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PciI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI SacI SacII SalI SanDI
SapI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI
ZraI