Restriction map for
pFN21AB5753
(Linear) (Six-base) MAPSORT of: KIBB5753.seq Check: 1089 from: 1 to: 2268
KIBB5753.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 443 961 979 1690 1870 2011 2268
Size: 428 15 518 18 711 180 141 257
Fragments arranged by size:
711 518 428 257 180 141 18 15
AflIII A'CryG_T
Cuts at: 0 1280 2268
Size: 1280 988
ApaLI G'TGCA_C
Cuts at: 0 838 1048 2268
Size: 838 210 1220
Fragments arranged by size:
1220 838 210
ApoI r'AATT_y
Cuts at: 0 702 774 1503 2111 2167 2257 2268
Size: 702 72 729 608 56 90 11
Fragments arranged by size:
729 702 608 90 72 56 11
AseI AT'TA_AT
Cuts at: 0 938 1733 2268
Size: 938 795 535
Fragments arranged by size:
938 795 535
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 683 716 2268
Size: 683 33 1552
Fragments arranged by size:
1552 683 33
BanII G_rGCy'C
Cuts at: 0 1144 2268
Size: 1144 1124
BbsI GAAGACnn'nnnn_
Cuts at: 0 1235 1529 1643 2268
Size: 1235 294 114 625
Fragments arranged by size:
1235 625 294 114
BciVI GTATCCnnnnn_n'
Cuts at: 0 1374 2268
Size: 1374 894
BfrBI ATG'CAT
Cuts at: 0 1459 2268
Size: 1459 809
BglI GCCn_nnn'nGGC
Cuts at: 0 49 2268
Size: 49 2219
BglII A'GATC_T
Cuts at: 0 13 332 748 2268
Size: 13 319 416 1520
Fragments arranged by size:
1520 416 319 13
Bme1580I G_kGCm'C
Cuts at: 0 842 1052 2268
Size: 842 210 1216
Fragments arranged by size:
1216 842 210
BmgBI CAC'GTC
Cuts at: 0 1283 2268
Size: 1283 985
BmrI ACTGGGnnnn_n'
Cuts at: 0 164 346 2268
Size: 164 182 1922
Fragments arranged by size:
1922 182 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1934 2268
Size: 1934 334
Bpu10I CC'TnA_GC
Cuts at: 0 26 2268
Size: 26 2242
BsaI GGTCTCn'nnnn_
Cuts at: 0 250 2268
Size: 250 2018
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 2268
Size: 2 2266
BsiHKAI G_wGCw'C
Cuts at: 0 296 842 1052 2268
Size: 296 546 210 1216
Fragments arranged by size:
1216 546 296 210
Bsp1286I G_dGCh'C
Cuts at: 0 296 842 1052 1144 2268
Size: 296 546 210 92 1124
Fragments arranged by size:
1124 546 296 210 92
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2224 2268
Size: 2224 44
BstEII G'GTnAC_C
Cuts at: 0 6 2268
Size: 6 2262
BstYI r'GATC_y
Cuts at: 0 13 118 332 516 748 2268
Size: 13 105 214 184 232 1520
Fragments arranged by size:
1520 232 214 184 105 13
BtsI GCAGTG_nn'
Cuts at: 0 1344 2268
Size: 1344 924
EaeI y'GGCC_r
Cuts at: 0 1407 2268
Size: 1407 861
EarI CTCTTCn'nnn_
Cuts at: 0 196 1196 1370 1511 1556 1562 2268
Size: 196 1000 174 141 45 6 706
Fragments arranged by size:
1000 706 196 174 141 45 6
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 443 961 979 1690 1870 1934 2011
Size: 428 15 518 18 711 180 64 77
Cuts at: 2011 2268
Size: 257
Fragments arranged by size:
711 518 428 257 180 77 64 18
15
EcoRI G'AATT_C
Cuts at: 0 702 2111 2167 2268
Size: 702 1409 56 101
Fragments arranged by size:
1409 702 101 56
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 790 822 2268
Size: 256 32 502 32 1446
Fragments arranged by size:
1446 502 256 32 32
HincII GTy'rAC
Cuts at: 0 1676 2082 2268
Size: 1676 406 186
Fragments arranged by size:
1676 406 186
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 2268
Size: 412 1856
MscI TGG'CCA
Cuts at: 0 1409 2268
Size: 1409 859
MspA1I CmG'CkG
Cuts at: 0 957 2092 2268
Size: 957 1135 176
Fragments arranged by size:
1135 957 176
NsiI A_TGCA'T
Cuts at: 0 1461 2268
Size: 1461 807
NspI r_CATG'y
Cuts at: 0 347 2268
Size: 347 1921
PfoI T'CCnGG_A
Cuts at: 0 1482 2268
Size: 1482 786
PshAI GACnn'nnGTC
Cuts at: 0 1697 2268
Size: 1697 571
PsiI TTA'TAA
Cuts at: 0 1216 2268
Size: 1216 1052
PstI C_TGCA'G
Cuts at: 0 1814 2268
Size: 1814 454
PvuII CAG'CTG
Cuts at: 0 957 2092 2268
Size: 957 1135 176
Fragments arranged by size:
1135 957 176
SapI GCTCTTCn'nnn_
Cuts at: 0 196 2268
Size: 196 2072
ScaI AGT'ACT
Cuts at: 0 114 2059 2194 2268
Size: 114 1945 135 74
Fragments arranged by size:
1945 135 114 74
SfcI C'TryA_G
Cuts at: 0 212 479 1753 1810 2268
Size: 212 267 1274 57 458
Fragments arranged by size:
1274 458 267 212 57
SpeI A'CTAG_T
Cuts at: 0 1326 2268
Size: 1326 942
SphI G_CATG'C
Cuts at: 0 347 2268
Size: 347 1921
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1708 2268
Size: 1708 560
TatI w'GTAC_w
Cuts at: 0 112 2057 2192 2268
Size: 112 1945 135 76
Fragments arranged by size:
1945 135 112 76
XbaI T'CTAG_A
Cuts at: 0 451 2268
Size: 451 1817
Enzymes that do cut:
AcuI AflIII ApaLI ApoI AseI BaeI BanII BbsI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaI BsgI BsiHKAI Bsp1286I BstAPI BstEII BstYI
BtsI EaeI EarI Eco57MI EcoRI Hin4I HincII MmeI
MscI MspA1I NsiI NspI PfoI PshAI PsiI PstI
PvuII SapI ScaI SfcI SpeI SphI TaqII TatI
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AsiSI
AvaI AvrII BamHI BanI BbeI BbvCI BcgI BclI
BlpI BmtI BplI BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI MslI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI PacI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SrfI
SspI StuI StyI SwaI TstI Tth111I XcmI XhoI
XmaI XmnI ZraI