Restriction map for
pFN21AB5745
(Linear) (Six-base) MAPSORT of: KIBB5745.seq Check: 8843 from: 1 to: 2379
KIBB5745.orf
With 182 enzymes: SgfI *
July 3, 2012 16:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1122 1294 1532 2379
Size: 1122 172 238 847
Fragments arranged by size:
1122 847 238 172
Acc65I G'GTAC_C
Cuts at: 0 1321 2379
Size: 1321 1058
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 395 678 1018 1978 2087 2379
Size: 227 168 283 340 960 109 292
Fragments arranged by size:
960 340 292 283 227 168 109
AfeI AGC'GCT
Cuts at: 0 1414 2379
Size: 1414 965
AflIII A'CryG_T
Cuts at: 0 491 1379 2379
Size: 491 888 1000
Fragments arranged by size:
1000 888 491
AgeI A'CCGG_T
Cuts at: 0 828 2379
Size: 828 1551
AleI CACnn'nnGTG
Cuts at: 0 1298 2379
Size: 1298 1081
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1431 2379
Size: 1431 948
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 306 338 2379
Size: 27 279 32 2041
Fragments arranged by size:
2041 279 32 27
AlwNI CAG_nnn'CTG
Cuts at: 0 250 850 2304 2379
Size: 250 600 1454 75
Fragments arranged by size:
1454 600 250 75
ApaI G_GGCC'C
Cuts at: 0 1798 2379
Size: 1798 581
ApaLI G'TGCA_C
Cuts at: 0 2161 2379
Size: 2161 218
ApoI r'AATT_y
Cuts at: 0 369 703 898 1532 1684 2379
Size: 369 334 195 634 152 695
Fragments arranged by size:
695 634 369 334 195 152
AseI AT'TA_AT
Cuts at: 0 100 2379
Size: 100 2279
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1313 1346 2379
Size: 1313 33 1033
Fragments arranged by size:
1313 1033 33
BamHI G'GATC_C
Cuts at: 0 1400 2379
Size: 1400 979
BanI G'GyrC_C
Cuts at: 0 183 1209 1321 2379
Size: 183 1026 112 1058
Fragments arranged by size:
1058 1026 183 112
BanII G_rGCy'C
Cuts at: 0 578 1798 2379
Size: 578 1220 581
Fragments arranged by size:
1220 581 578
BbsI GAAGACnn'nnnn_
Cuts at: 0 1440 2244 2379
Size: 1440 804 135
Fragments arranged by size:
1440 804 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1212 1246 2379
Size: 1212 34 1133
Fragments arranged by size:
1212 1133 34
BclI T'GATC_A
Cuts at: 0 1697 2099 2379
Size: 1697 402 280
Fragments arranged by size:
1697 402 280
BfrBI ATG'CAT
Cuts at: 0 1359 2353 2379
Size: 1359 994 26
Fragments arranged by size:
1359 994 26
Bme1580I G_kGCm'C
Cuts at: 0 1410 1798 1905 2165 2205 2379
Size: 1410 388 107 260 40 174
Fragments arranged by size:
1410 388 260 174 107 40
BmgBI CAC'GTC
Cuts at: 0 1380 2379
Size: 1380 999
BmrI ACTGGGnnnn_n'
Cuts at: 0 1020 1899 2075 2379
Size: 1020 879 176 304
Fragments arranged by size:
1020 879 304 176
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1489 1897 2239 2379
Size: 377 1112 408 342 140
Fragments arranged by size:
1112 408 377 342 140
Bpu10I CC'TnA_GC
Cuts at: 0 2206 2379
Size: 2206 173
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1219 1228 2379
Size: 1219 9 1151
Fragments arranged by size:
1219 1151 9
BsaI GGTCTCn'nnnn_
Cuts at: 0 1988 2379
Size: 1988 391
BsaBI GATnn'nnATC
Cuts at: 0 2104 2379
Size: 2104 275
BsaWI w'CCGG_w
Cuts at: 0 665 828 2379
Size: 665 163 1551
Fragments arranged by size:
1551 665 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 390 420 1003 1033 2379
Size: 390 30 583 30 1346
Fragments arranged by size:
1346 583 390 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1588 1591 1894 1981 2379
Size: 1588 3 303 87 398
Fragments arranged by size:
1588 398 303 87 3
BseYI C'CCAG_C
Cuts at: 0 520 2379
Size: 520 1859
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 893 1322 2140 2356 2379
Size: 893 429 818 216 23
Fragments arranged by size:
893 818 429 216 23
BsiEI CG_ry'CG
Cuts at: 0 558 1226 2379
Size: 558 668 1153
Fragments arranged by size:
1153 668 558
BsiHKAI G_wGCw'C
Cuts at: 0 2165 2212 2379
Size: 2165 47 167
Fragments arranged by size:
2165 167 47
BsmI GAATG_Cn'
Cuts at: 0 2353 2379
Size: 2353 26
Bsp1286I G_dGCh'C
Cuts at: 0 578 1410 1798 1905 2165 2205 2212 2379
Size: 578 832 388 107 260 40 7 167
Fragments arranged by size:
832 578 388 260 167 107 40 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1122 1294 1532 2379
Size: 1122 172 238 847
Fragments arranged by size:
1122 847 238 172
BsrDI GCAATG_nn'
Cuts at: 0 902 2379
Size: 902 1477
BsrFI r'CCGG_y
Cuts at: 0 828 1763 2030 2379
Size: 828 935 267 349
Fragments arranged by size:
935 828 349 267
BsrGI T'GTAC_A
Cuts at: 0 488 2379
Size: 488 1891
BstEII G'GTnAC_C
Cuts at: 0 880 987 2004 2379
Size: 880 107 1017 375
Fragments arranged by size:
1017 880 375 107
BstXI CCAn_nnnn'nTGG
Cuts at: 0 984 2056 2379
Size: 984 1072 323
Fragments arranged by size:
1072 984 323
BstYI r'GATC_y
Cuts at: 0 601 1400 2036 2379
Size: 601 799 636 343
Fragments arranged by size:
799 636 601 343
BtgI C'CryG_G
Cuts at: 0 638 2055 2379
Size: 638 1417 324
Fragments arranged by size:
1417 638 324
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2094 2379
Size: 2094 285
BtsI GCAGTG_nn'
Cuts at: 0 39 1293 1305 2163 2379
Size: 39 1254 12 858 216
Fragments arranged by size:
1254 858 216 39 12
ClaI AT'CG_AT
Cuts at: 0 780 2379
Size: 780 1599
DrdI GACnn_nn'nnGTC
Cuts at: 0 2002 2379
Size: 2002 377
EaeI y'GGCC_r
Cuts at: 0 555 596 1033 1195 1912 2379
Size: 555 41 437 162 717 467
Fragments arranged by size:
717 555 467 437 162 41
EagI C'GGCC_G
Cuts at: 0 555 2379
Size: 555 1824
EarI CTCTTCn'nnn_
Cuts at: 0 128 647 697 1715 1805 2379
Size: 128 519 50 1018 90 574
Fragments arranged by size:
1018 574 519 128 90 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 963 2379
Size: 963 1416
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 377 395 678 1018 1489 1897 1978
Size: 227 150 18 283 340 471 408 81
Cuts at: 1978 2087 2239 2379
Size: 109 152 140
Fragments arranged by size:
471 408 340 283 227 152 150 140
109 81 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 83 1246 2379
Size: 83 1163 1133
Fragments arranged by size:
1163 1133 83
EcoO109I rG'GnC_Cy
Cuts at: 0 214 1794 1795 2192 2379
Size: 214 1580 1 397 187
Fragments arranged by size:
1580 397 214 187 1
EcoRI G'AATT_C
Cuts at: 0 369 703 1684 2379
Size: 369 334 981 695
Fragments arranged by size:
981 695 369 334
HaeII r_GCGC'y
Cuts at: 0 1167 1416 2174 2379
Size: 1167 249 758 205
Fragments arranged by size:
1167 758 249 205
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1831 1863 2341 2373 2379
Size: 1831 32 478 32 6
Fragments arranged by size:
1831 478 32 32 6
HincII GTy'rAC
Cuts at: 0 1592 2379
Size: 1592 787
HpaI GTT'AAC
Cuts at: 0 1592 2379
Size: 1592 787
KpnI G_GTAC'C
Cuts at: 0 1325 2379
Size: 1325 1054
MfeI C'AATT_G
Cuts at: 0 1811 2379
Size: 1811 568
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 575 2379
Size: 178 397 1804
Fragments arranged by size:
1804 397 178
MscI TGG'CCA
Cuts at: 0 598 1035 1197 1914 2379
Size: 598 437 162 717 465
Fragments arranged by size:
717 598 465 437 162
MslI CAynn'nnrTG
Cuts at: 0 982 1298 2054 2158 2379
Size: 982 316 756 104 221
Fragments arranged by size:
982 756 316 221 104
MspA1I CmG'CkG
Cuts at: 0 1193 2379
Size: 1193 1186
NaeI GCC'GGC
Cuts at: 0 2032 2379
Size: 2032 347
NcoI C'CATG_G
Cuts at: 0 2055 2379
Size: 2055 324
NdeI CA'TA_TG
Cuts at: 0 1680 2379
Size: 1680 699
NgoMIV G'CCGG_C
Cuts at: 0 2030 2379
Size: 2030 349
NsiI A_TGCA'T
Cuts at: 0 1361 2355 2379
Size: 1361 994 24
Fragments arranged by size:
1361 994 24
NspI r_CATG'y
Cuts at: 0 495 725 1233 2379
Size: 495 230 508 1146
Fragments arranged by size:
1146 508 495 230
PacI TTA_AT'TAA
Cuts at: 0 104 2379
Size: 104 2275
PasI CC'CwG_GG
Cuts at: 0 359 1549 2379
Size: 359 1190 830
Fragments arranged by size:
1190 830 359
PciI A'CATG_T
Cuts at: 0 491 2379
Size: 491 1888
PflMI CCAn_nnn'nTGG
Cuts at: 0 1134 1204 1921 2304 2379
Size: 1134 70 717 383 75
Fragments arranged by size:
1134 717 383 75 70
PfoI T'CCnGG_A
Cuts at: 0 2216 2379
Size: 2216 163
PpuMI rG'GwC_Cy
Cuts at: 0 214 2192 2379
Size: 214 1978 187
Fragments arranged by size:
1978 214 187
PshAI GACnn'nnGTC
Cuts at: 0 1929 2379
Size: 1929 450
PspOMI G'GGCC_C
Cuts at: 0 1794 2379
Size: 1794 585
PstI C_TGCA'G
Cuts at: 0 1300 1529 2170 2379
Size: 1300 229 641 209
Fragments arranged by size:
1300 641 229 209
PvuI CG_AT'CG
Cuts at: 0 1226 2379
Size: 1226 1153
PvuII CAG'CTG
Cuts at: 0 1193 2379
Size: 1193 1186
SanDI GG'GwC_CC
Cuts at: 0 214 2379
Size: 214 2165
SapI GCTCTTCn'nnn_
Cuts at: 0 697 2379
Size: 697 1682
ScaI AGT'ACT
Cuts at: 0 1030 1909 2379
Size: 1030 879 470
Fragments arranged by size:
1030 879 470
SfcI C'TryA_G
Cuts at: 0 83 287 1076 1296 1525 2166 2278 2379
Size: 83 204 789 220 229 641 112 101
Fragments arranged by size:
789 641 229 220 204 112 101 83
SmlI C'TyrA_G
Cuts at: 0 1234 1243 2379
Size: 1234 9 1136
Fragments arranged by size:
1234 1136 9
SphI G_CATG'C
Cuts at: 0 1233 2379
Size: 1233 1146
SspI AAT'ATT
Cuts at: 0 1065 2379
Size: 1065 1314
StuI AGG'CCT
Cuts at: 0 1258 2379
Size: 1258 1121
StyI C'CwwG_G
Cuts at: 0 755 1041 2055 2379
Size: 755 286 1014 324
Fragments arranged by size:
1014 755 324 286
TatI w'GTAC_w
Cuts at: 0 488 1028 1907 2379
Size: 488 540 879 472
Fragments arranged by size:
879 540 488 472
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1003 1035 2184 2216 2279 2311 2379
Size: 1003 32 1149 32 63 32 68
Fragments arranged by size:
1149 1003 68 63 32 32 32
XbaI T'CTAG_A
Cuts at: 0 811 1512 2379
Size: 811 701 867
Fragments arranged by size:
867 811 701
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 314 981 2379
Size: 314 667 1398
Fragments arranged by size:
1398 667 314
XmnI GAAnn'nnTTC
Cuts at: 0 839 898 940 2379
Size: 839 59 42 1439
Fragments arranged by size:
1439 839 59 42
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AleI AlfI
AloI AlwNI ApaI ApaLI ApoI AseI BaeI BamHI
BanI BanII BbsI BcgI BclI BfrBI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI KpnI
MfeI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NsiI NspI PacI PasI PciI PflMI PfoI
PpuMI PshAI PspOMI PstI PvuI PvuII SanDI SapI
ScaI SfcI SmlI SphI SspI StuI StyI TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AscI AsiSI AvaI
AvrII BbeI BbvCI BciVI BglI BglII BlpI BmtI
BplI BsaAI BsaHI BsiWI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstAPI BstBI BstZ17I Bsu36I CspCI DraI
DraIII EcoICRI EcoRV FalI FseI FspI FspAI HindIII
KasI MluI NarI NheI NotI NruI PmeI PmlI
PpiI PsiI PspXI PsrI RsrII SacI SacII SalI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI TaqII Tth111I XhoI XmaI ZraI