Restriction map for
pFN21AB5742
(Linear) (Six-base) MAPSORT of: KIBB5742.seq Check: 3808 from: 1 to: 2223
KIBB5742.orf
With 182 enzymes: SgfI *
July 3, 2012 16:04 ..
AccI GT'mk_AC
Cuts at: 0 2108 2223
Size: 2108 115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 874 1019 1060 1106 2223
Size: 874 145 41 46 1117
Fragments arranged by size:
1117 874 145 46 41
AflIII A'CryG_T
Cuts at: 0 537 1713 2223
Size: 537 1176 510
Fragments arranged by size:
1176 537 510
AhdI GACnn_n'nnGTC
Cuts at: 0 1579 2223
Size: 1579 644
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 218 250 2223
Size: 218 32 1973
Fragments arranged by size:
1973 218 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1320 1455 2223
Size: 1320 135 768
Fragments arranged by size:
1320 768 135
ApaLI G'TGCA_C
Cuts at: 0 1199 1339 2223
Size: 1199 140 884
Fragments arranged by size:
1199 884 140
ApoI r'AATT_y
Cuts at: 0 424 453 1375 2001 2223
Size: 424 29 922 626 222
Fragments arranged by size:
922 626 424 222 29
AseI AT'TA_AT
Cuts at: 0 797 1907 2223
Size: 797 1110 316
Fragments arranged by size:
1110 797 316
BanII G_rGCy'C
Cuts at: 0 637 1021 2223
Size: 637 384 1202
Fragments arranged by size:
1202 637 384
BbsI GAAGACnn'nnnn_
Cuts at: 0 77 724 1007 1180 2086 2223
Size: 77 647 283 173 906 137
Fragments arranged by size:
906 647 283 173 137 77
BbvCI CC'TCA_GC
Cuts at: 0 490 2223
Size: 490 1733
BciVI GTATCCnnnnn_n'
Cuts at: 0 1432 2223
Size: 1432 791
BglI GCCn_nnn'nGGC
Cuts at: 0 1280 2223
Size: 1280 943
BglII A'GATC_T
Cuts at: 0 1557 2091 2223
Size: 1557 534 132
Fragments arranged by size:
1557 534 132
Bme1580I G_kGCm'C
Cuts at: 0 1203 1343 2223
Size: 1203 140 880
Fragments arranged by size:
1203 880 140
BmgBI CAC'GTC
Cuts at: 0 41 2223
Size: 41 2182
BmrI ACTGGGnnnn_n'
Cuts at: 0 1035 2223
Size: 1035 1188
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 796 1553 1646 2223
Size: 30 766 757 93 577
Fragments arranged by size:
766 757 577 93 30
Bpu10I CC'TnA_GC
Cuts at: 0 490 631 2223
Size: 490 141 1592
Fragments arranged by size:
1592 490 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 2223
Size: 1066 1157
BsaI GGTCTCn'nnnn_
Cuts at: 0 2051 2223
Size: 2051 172
BsaAI yAC'GTr
Cuts at: 0 540 1198 2223
Size: 540 658 1025
Fragments arranged by size:
1025 658 540
BsaWI w'CCGG_w
Cuts at: 0 1997 2223
Size: 1997 226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 134 164 218 248 335 365 434 464
Size: 134 30 54 30 87 30 69 30
Cuts at: 464 2223
Size: 1759
Fragments arranged by size:
1759 134 87 69 54 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 455 2223
Size: 455 1768
BseYI C'CCAG_C
Cuts at: 0 1740 2223
Size: 1740 483
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 2223
Size: 784 1439
BsiHKAI G_wGCw'C
Cuts at: 0 637 788 1021 1203 1343 2223
Size: 637 151 233 182 140 880
Fragments arranged by size:
880 637 233 182 151 140
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1699 2223
Size: 1699 524
Bsp1286I G_dGCh'C
Cuts at: 0 637 788 1021 1203 1343 2223
Size: 637 151 233 182 140 880
Fragments arranged by size:
880 637 233 182 151 140
BspEI T'CCGG_A
Cuts at: 0 1997 2223
Size: 1997 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1745 2223
Size: 1745 478
BsrBI CCG'CTC
Cuts at: 0 486 2223
Size: 486 1737
BstAPI GCAn_nnn'nTGC
Cuts at: 0 301 672 2223
Size: 301 371 1551
Fragments arranged by size:
1551 371 301
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2205 2223
Size: 2205 18
BstYI r'GATC_y
Cuts at: 0 906 1044 1452 1536 1557 1620 1838 2091
Size: 906 138 408 84 21 63 218 253
Cuts at: 2091 2223
Size: 132
Fragments arranged by size:
906 408 253 218 138 132 84 63
21
BtgI C'CryG_G
Cuts at: 0 681 2223
Size: 681 1542
BtsI GCAGTG_nn'
Cuts at: 0 71 743 826 2223
Size: 71 672 83 1397
Fragments arranged by size:
1397 672 83 71
DraIII CAC_nnn'GTG
Cuts at: 0 1251 2223
Size: 1251 972
EaeI y'GGCC_r
Cuts at: 0 2207 2223
Size: 2207 16
EarI CTCTTCn'nnn_
Cuts at: 0 158 1637 1702 2223
Size: 158 1479 65 521
Fragments arranged by size:
1479 521 158 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 942 2223
Size: 942 1281
EcoICRI GAG'CTC
Cuts at: 0 635 1019 2223
Size: 635 384 1204
Fragments arranged by size:
1204 635 384
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 30 796 874 1019 1060 1106 1553 1646
Size: 30 766 78 145 41 46 447 93
Cuts at: 1646 2223
Size: 577
Fragments arranged by size:
766 577 447 145 93 78 46 41
30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 949 2223
Size: 949 1274
EcoO109I rG'GnC_Cy
Cuts at: 0 34 1566 1680 2223
Size: 34 1532 114 543
Fragments arranged by size:
1532 543 114 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 768 2223
Size: 768 1455
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 335 367 609 641 1565 1597 1710
Size: 29 306 32 242 32 924 32 113
Cuts at: 1710 1742 2223
Size: 32 481
Fragments arranged by size:
924 481 306 242 113 32 32 32
32 29
HincII GTy'rAC
Cuts at: 0 114 2223
Size: 114 2109
MfeI C'AATT_G
Cuts at: 0 127 2223
Size: 127 2096
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1832 2223
Size: 1832 391
MscI TGG'CCA
Cuts at: 0 2209 2223
Size: 2209 14
MslI CAynn'nnrTG
Cuts at: 0 2018 2223
Size: 2018 205
MspA1I CmG'CkG
Cuts at: 0 50 397 1206 2223
Size: 50 347 809 1017
Fragments arranged by size:
1017 809 347 50
NcoI C'CATG_G
Cuts at: 0 681 2223
Size: 681 1542
NspI r_CATG'y
Cuts at: 0 1717 2223
Size: 1717 506
PasI CC'CwG_GG
Cuts at: 0 2215 2223
Size: 2215 8
PciI A'CATG_T
Cuts at: 0 1713 2223
Size: 1713 510
PfoI T'CCnGG_A
Cuts at: 0 158 2223
Size: 158 2065
PmlI CAC'GTG
Cuts at: 0 1198 2223
Size: 1198 1025
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 2223
Size: 218 32 1973
Fragments arranged by size:
1973 218 32
PpuMI rG'GwC_Cy
Cuts at: 0 34 1566 1680 2223
Size: 34 1532 114 543
Fragments arranged by size:
1532 543 114 34
PshAI GACnn'nnGTC
Cuts at: 0 39 2223
Size: 39 2184
PsiI TTA'TAA
Cuts at: 0 434 2223
Size: 434 1789
PstI C_TGCA'G
Cuts at: 0 66 2223
Size: 66 2157
SacI G_AGCT'C
Cuts at: 0 637 1021 2223
Size: 637 384 1202
Fragments arranged by size:
1202 637 384
SanDI GG'GwC_CC
Cuts at: 0 34 2223
Size: 34 2189
SexAI A'CCwGG_T
Cuts at: 0 116 2223
Size: 116 2107
SfcI C'TryA_G
Cuts at: 0 62 1776 2119 2223
Size: 62 1714 343 104
Fragments arranged by size:
1714 343 104 62
SmlI C'TyrA_G
Cuts at: 0 1081 2223
Size: 1081 1142
StyI C'CwwG_G
Cuts at: 0 681 1569 2223
Size: 681 888 654
Fragments arranged by size:
888 681 654
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1459 1966 2207 2223
Size: 1459 507 241 16
Fragments arranged by size:
1459 507 241 16
TatI w'GTAC_w
Cuts at: 0 198 892 2223
Size: 198 694 1331
Fragments arranged by size:
1331 694 198
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 434 466 2223
Size: 434 32 1757
Fragments arranged by size:
1757 434 32
Tth111I GACn'n_nGTC
Cuts at: 0 118 2223
Size: 118 2105
XbaI T'CTAG_A
Cuts at: 0 1560 2223
Size: 1560 663
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 513 2223
Size: 513 1710
XmnI GAAnn'nnTTC
Cuts at: 0 328 983 1177 2223
Size: 328 655 194 1046
Fragments arranged by size:
1046 655 328 194
Enzymes that do cut:
AccI AcuI AflIII AhdI AloI AlwNI ApaLI ApoI
AseI BanII BbsI BbvCI BciVI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrBI BstAPI BstXI BstYI BtgI BtsI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I HincII MfeI MmeI MscI MslI MspA1I NcoI
NspI PasI PciI PfoI PmlI PpiI PpuMI PshAI
PsiI PstI SacI SanDI SexAI SfcI SmlI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AlfI ApaI AscI AsiSI AvaI AvrII BaeI BamHI
BanI BbeI BcgI BclI BfrBI BlpI BmtI BplI
BsaBI BsaHI BsiEI BsiWI BsmI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DrdI EagI EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MluI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PflMI PmeI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SapI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI XhoI XmaI ZraI