Restriction map for
pFN21AB5739
(Linear) (Six-base) MAPSORT of: KIBB5739.seq Check: 6864 from: 1 to: 2367
KIBB5739.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2227 2367
Size: 2227 140
AclI AA'CG_TT
Cuts at: 0 1754 2367
Size: 1754 613
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 362 940 1345 1353 1663 1837 2367
Size: 362 578 405 8 310 174 530
Fragments arranged by size:
578 530 405 362 310 174 8
AflIII A'CryG_T
Cuts at: 0 1079 2367
Size: 1079 1288
AhdI GACnn_n'nnGTC
Cuts at: 0 2295 2367
Size: 2295 72
AlwNI CAG_nnn'CTG
Cuts at: 0 465 1279 1416 2016 2219 2237 2367
Size: 465 814 137 600 203 18 130
Fragments arranged by size:
814 600 465 203 137 130 18
ApoI r'AATT_y
Cuts at: 0 218 299 425 2367
Size: 218 81 126 1942
Fragments arranged by size:
1942 218 126 81
AvaI C'yCGr_G
Cuts at: 0 1583 2367
Size: 1583 784
BanI G'GyrC_C
Cuts at: 0 9 1959 2367
Size: 9 1950 408
Fragments arranged by size:
1950 408 9
BanII G_rGCy'C
Cuts at: 0 2333 2367
Size: 2333 34
BbeI G_GCGC'C
Cuts at: 0 13 2367
Size: 13 2354
BbsI GAAGACnn'nnnn_
Cuts at: 0 610 2367
Size: 610 1757
BbvCI CC'TCA_GC
Cuts at: 0 224 2367
Size: 224 2143
BciVI GTATCCnnnnn_n'
Cuts at: 0 514 711 762 2367
Size: 514 197 51 1605
Fragments arranged by size:
1605 514 197 51
BfrBI ATG'CAT
Cuts at: 0 1122 1762 2367
Size: 1122 640 605
Fragments arranged by size:
1122 640 605
BglI GCCn_nnn'nGGC
Cuts at: 0 64 2367
Size: 64 2303
Bme1580I G_kGCm'C
Cuts at: 0 943 1964 2367
Size: 943 1021 403
Fragments arranged by size:
1021 943 403
BmgBI CAC'GTC
Cuts at: 0 1082 2367
Size: 1082 1285
BmrI ACTGGGnnnn_n'
Cuts at: 0 1098 2367
Size: 1098 1269
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2250 2367
Size: 2250 117
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 1696 2367
Size: 50 1646 671
Fragments arranged by size:
1646 671 50
Bpu10I CC'TnA_GC
Cuts at: 0 224 2367
Size: 224 2143
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 277 519 849 1426 2367
Size: 220 57 242 330 577 941
Fragments arranged by size:
941 577 330 242 220 57
BsaAI yAC'GTr
Cuts at: 0 2007 2367
Size: 2007 360
BsaBI GATnn'nnATC
Cuts at: 0 448 2367
Size: 448 1919
BsaHI Gr'CG_yC
Cuts at: 0 10 1179 2367
Size: 10 1169 1188
Fragments arranged by size:
1188 1169 10
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2254 2284 2367
Size: 2254 30 83
Fragments arranged by size:
2254 83 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 296 1045 1075 2341 2367
Size: 223 73 749 30 1266 26
Fragments arranged by size:
1266 749 223 73 30 26
BseYI C'CCAG_C
Cuts at: 0 22 1094 2367
Size: 22 1072 1273
Fragments arranged by size:
1273 1072 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 226 2029 2182 2367
Size: 184 42 1803 153 185
Fragments arranged by size:
1803 185 184 153 42
BsiEI CG_ry'CG
Cuts at: 0 39 52 90 94 144 2367
Size: 39 13 38 4 50 2223
Fragments arranged by size:
2223 50 39 38 13 4
BsiHKAI G_wGCw'C
Cuts at: 0 1043 1151 1664 1712 2126 2367
Size: 1043 108 513 48 414 241
Fragments arranged by size:
1043 513 414 241 108 48
BsmI GAATG_Cn'
Cuts at: 0 878 1269 1762 1936 2367
Size: 878 391 493 174 431
Fragments arranged by size:
878 493 431 391 174
BsmBI CGTCTCn'nnnn_
Cuts at: 0 39 2367
Size: 39 2328
Bsp1286I G_dGCh'C
Cuts at: 0 943 1043 1151 1664 1712 1964 2126 2333
Size: 943 100 108 513 48 252 162 207
Cuts at: 2333 2367
Size: 34
Fragments arranged by size:
943 513 252 207 162 108 100 48
34
BspHI T'CATG_A
Cuts at: 0 1538 2367
Size: 1538 829
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1524 2108 2177 2204 2227 2367
Size: 1524 584 69 27 23 140
Fragments arranged by size:
1524 584 140 69 27 23
BsrBI CCG'CTC
Cuts at: 0 897 2085 2367
Size: 897 1188 282
Fragments arranged by size:
1188 897 282
BsrFI r'CCGG_y
Cuts at: 0 12 34 89 139 2291 2367
Size: 12 22 55 50 2152 76
Fragments arranged by size:
2152 76 55 50 22 12
BsrGI T'GTAC_A
Cuts at: 0 526 1133 2367
Size: 526 607 1234
Fragments arranged by size:
1234 607 526
BssSI C'ACGA_G
Cuts at: 0 1381 2367
Size: 1381 986
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2196 2367
Size: 2196 171
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1854 2367
Size: 1854 513
Bsu36I CC'TnA_GG
Cuts at: 0 1457 2367
Size: 1457 910
BtgI C'CryG_G
Cuts at: 0 94 317 1293 2367
Size: 94 223 976 1074
Fragments arranged by size:
1074 976 223 94
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 190 195 225 230 2367
Size: 190 5 30 5 2137
Fragments arranged by size:
2137 190 30 5 5
EaeI y'GGCC_r
Cuts at: 0 36 87 91 141 1022 2367
Size: 36 51 4 50 881 1345
Fragments arranged by size:
1345 881 51 50 36 4
EagI C'GGCC_G
Cuts at: 0 36 87 91 141 2367
Size: 36 51 4 50 2226
Fragments arranged by size:
2226 51 50 36 4
EarI CTCTTCn'nnn_
Cuts at: 0 314 338 817 962 1501 1987 2367
Size: 314 24 479 145 539 486 380
Fragments arranged by size:
539 486 479 380 314 145 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 92 1975 2367
Size: 92 1883 392
Fragments arranged by size:
1883 392 92
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 362 940 1345 1353 1663 1696 1837
Size: 50 312 578 405 8 310 33 141
Cuts at: 1837 2367
Size: 530
Fragments arranged by size:
578 530 405 312 310 141 50 33
8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2200 2367
Size: 2200 167
EcoO109I rG'GnC_Cy
Cuts at: 0 147 838 2367
Size: 147 691 1529
Fragments arranged by size:
1529 691 147
EcoRI G'AATT_C
Cuts at: 0 218 2367
Size: 218 2149
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 513 582 627 843 886 1530 2367
Size: 513 69 45 216 43 644 837
Fragments arranged by size:
837 644 513 216 69 45 43
FseI GG_CCGG'CC
Cuts at: 0 93 2367
Size: 93 2274
HaeII r_GCGC'y
Cuts at: 0 13 2367
Size: 13 2354
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 187 219 469 501 1164 1196 1681 1713
Size: 187 32 250 32 663 32 485 32
Cuts at: 1713 1909 1941 2254 2281 2286 2313 2367
Size: 196 32 313 27 5 27 54
Fragments arranged by size:
663 485 313 250 196 187 54 32
32 32 32 32 27 27 5
HincII GTy'rAC
Cuts at: 0 935 2367
Size: 935 1432
KasI G'GCGC_C
Cuts at: 0 9 2367
Size: 9 2358
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 690 944 1871 2367
Size: 690 254 927 496
Fragments arranged by size:
927 690 496 254
MscI TGG'CCA
Cuts at: 0 1024 2367
Size: 1024 1343
MslI CAynn'nnrTG
Cuts at: 0 1855 1969 2005 2367
Size: 1855 114 36 362
Fragments arranged by size:
1855 362 114 36
MspA1I CmG'CkG
Cuts at: 0 96 120 135 228 850 1098 2036 2115
Size: 96 24 15 93 622 248 938 79
Cuts at: 2115 2367
Size: 252
Fragments arranged by size:
938 622 252 248 96 93 79 24
15
NaeI GCC'GGC
Cuts at: 0 36 91 2367
Size: 36 55 2276
Fragments arranged by size:
2276 55 36
NarI GG'CG_CC
Cuts at: 0 10 2367
Size: 10 2357
NcoI C'CATG_G
Cuts at: 0 317 1293 2367
Size: 317 976 1074
Fragments arranged by size:
1074 976 317
NgoMIV G'CCGG_C
Cuts at: 0 34 89 2367
Size: 34 55 2278
Fragments arranged by size:
2278 55 34
NsiI A_TGCA'T
Cuts at: 0 1124 1764 2367
Size: 1124 640 603
Fragments arranged by size:
1124 640 603
NspI r_CATG'y
Cuts at: 0 1854 2367
Size: 1854 513
PmlI CAC'GTG
Cuts at: 0 2007 2367
Size: 2007 360
PstI C_TGCA'G
Cuts at: 0 119 849 1535 2114 2367
Size: 119 730 686 579 253
Fragments arranged by size:
730 686 579 253 119
PvuII CAG'CTG
Cuts at: 0 120 135 228 850 1098 2036 2367
Size: 120 15 93 622 248 938 331
Fragments arranged by size:
938 622 331 248 120 93 15
RsrII CG'GwC_CG
Cuts at: 0 2289 2367
Size: 2289 78
SacII CC_GC'GG
Cuts at: 0 97 2367
Size: 97 2270
SapI GCTCTTCn'nnn_
Cuts at: 0 817 2367
Size: 817 1550
ScaI AGT'ACT
Cuts at: 0 1393 2367
Size: 1393 974
SexAI A'CCwGG_T
Cuts at: 0 1190 1895 2367
Size: 1190 705 472
Fragments arranged by size:
1190 705 472
SfcI C'TryA_G
Cuts at: 0 115 845 1317 1531 2110 2367
Size: 115 730 472 214 579 257
Fragments arranged by size:
730 579 472 257 214 115
SfoI GGC'GCC
Cuts at: 0 11 2367
Size: 11 2356
SmlI C'TyrA_G
Cuts at: 0 235 292 498 828 1441 2367
Size: 235 57 206 330 613 926
Fragments arranged by size:
926 613 330 235 206 57
StuI AGG'CCT
Cuts at: 0 438 1652 2367
Size: 438 1214 715
Fragments arranged by size:
1214 715 438
StyI C'CwwG_G
Cuts at: 0 317 488 1293 2367
Size: 317 171 805 1074
Fragments arranged by size:
1074 805 317 171
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 634 1898 2367
Size: 634 1264 469
Fragments arranged by size:
1264 634 469
TatI w'GTAC_w
Cuts at: 0 526 1133 1391 2367
Size: 526 607 258 976
Fragments arranged by size:
976 607 526 258
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1675 1707 2367
Size: 1675 32 660
Fragments arranged by size:
1675 660 32
Tth111I GACn'n_nGTC
Cuts at: 0 2268 2367
Size: 2268 99
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 324 762 2367
Size: 324 438 1605
Fragments arranged by size:
1605 438 324
XmnI GAAnn'nnTTC
Cuts at: 0 607 2367
Size: 607 1760
Enzymes that do cut:
AarI AclI AcuI AflIII AhdI AlwNI ApoI AvaI
BanI BanII BbeI BbsI BbvCI BciVI BfrBI BglI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI
BssSI BstAPI BstXI Bsu36I BtgI CspCI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PmlI PstI
PvuII RsrII SacII SapI ScaI SexAI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflII AgeI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BcgI BclI BglII BlpI BmtI BsaI BsaWI
BsiWI BspEI BsrDI BssHII BstBI BstEII BstYI BstZ17I
BtgZI BtsI ClaI DraI DraIII DrdI EcoICRI EcoRV
FspI FspAI HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI SacI SalI SanDI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI XmaI ZraI