Restriction map for
pFN21AB5712
(Linear) (Six-base) MAPSORT of: KIBB5712.seq Check: 6350 from: 1 to: 2160
KIBB5712.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 591 735 1665 2160
Size: 591 144 930 495
Fragments arranged by size:
930 591 495 144
AatII G_ACGT'C
Cuts at: 0 965 2160
Size: 965 1195
Acc65I G'GTAC_C
Cuts at: 0 392 1313 2160
Size: 392 921 847
Fragments arranged by size:
921 847 392
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 862 977 2051 2120 2160
Size: 862 115 1074 69 40
Fragments arranged by size:
1074 862 115 69 40
AgeI A'CCGG_T
Cuts at: 0 6 2160
Size: 6 2154
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 127 1495 2160
Size: 127 1368 665
Fragments arranged by size:
1368 665 127
AlwNI CAG_nnn'CTG
Cuts at: 0 207 490 891 1024 1791 1908 2160
Size: 207 283 401 133 767 117 252
Fragments arranged by size:
767 401 283 252 207 133 117
ApaI G_GGCC'C
Cuts at: 0 951 1264 1530 2160
Size: 951 313 266 630
Fragments arranged by size:
951 630 313 266
ApaLI G'TGCA_C
Cuts at: 0 1858 2160
Size: 1858 302
ApoI r'AATT_y
Cuts at: 0 632 2160
Size: 632 1528
AvaI C'yCGr_G
Cuts at: 0 136 595 1538 2160
Size: 136 459 943 622
Fragments arranged by size:
943 622 459 136
AvrII C'CTAG_G
Cuts at: 0 1773 2160
Size: 1773 387
BanI G'GyrC_C
Cuts at: 0 392 1242 1271 1313 2160
Size: 392 850 29 42 847
Fragments arranged by size:
850 847 392 42 29
BanII G_rGCy'C
Cuts at: 0 176 214 916 951 1046 1109 1264 1530
Size: 176 38 702 35 95 63 155 266
Cuts at: 1530 1816 2160
Size: 286 344
Fragments arranged by size:
702 344 286 266 176 155 95 63
38 35
BbeI G_GCGC'C
Cuts at: 0 1275 2160
Size: 1275 885
BbsI GAAGACnn'nnnn_
Cuts at: 0 965 974 1553 2160
Size: 965 9 579 607
Fragments arranged by size:
965 607 579 9
BbvCI CC'TCA_GC
Cuts at: 0 1340 1750 1841 2160
Size: 1340 410 91 319
Fragments arranged by size:
1340 410 319 91
BclI T'GATC_A
Cuts at: 0 1386 2160
Size: 1386 774
BglII A'GATC_T
Cuts at: 0 1464 2160
Size: 1464 696
BlpI GC'TnA_GC
Cuts at: 0 385 457 1171 2160
Size: 385 72 714 989
Fragments arranged by size:
989 714 385 72
Bme1580I G_kGCm'C
Cuts at: 0 505 951 1264 1530 1862 2160
Size: 505 446 313 266 332 298
Fragments arranged by size:
505 446 332 313 298 266
BmrI ACTGGGnnnn_n'
Cuts at: 0 986 2160
Size: 986 1174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2010 2160
Size: 2010 150
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 184 198 737 1085 1502 2160
Size: 100 84 14 539 348 417 658
Fragments arranged by size:
658 539 417 348 100 84 14
Bpu10I CC'TnA_GC
Cuts at: 0 208 1040 1340 1750 1841 1940 2160
Size: 208 832 300 410 91 99 220
Fragments arranged by size:
832 410 300 220 208 99 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1276 1607 1873 2160
Size: 1276 331 266 287
Fragments arranged by size:
1276 331 287 266
BsaI GGTCTCn'nnnn_
Cuts at: 0 7 161 856 1555 1829 2160
Size: 7 154 695 699 274 331
Fragments arranged by size:
699 695 331 274 154 7
BsaAI yAC'GTr
Cuts at: 0 528 2160
Size: 528 1632
BsaHI Gr'CG_yC
Cuts at: 0 368 877 962 1272 2160
Size: 368 509 85 310 888
Fragments arranged by size:
888 509 368 310 85
BsaWI w'CCGG_w
Cuts at: 0 6 858 2160
Size: 6 852 1302
Fragments arranged by size:
1302 852 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 385 415 710 740 1012 1042 2160
Size: 385 30 295 30 272 30 1118
Fragments arranged by size:
1118 385 295 272 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 364 466 775 924 2160
Size: 364 102 309 149 1236
Fragments arranged by size:
1236 364 309 149 102
BseYI C'CCAG_C
Cuts at: 0 532 1003 1068 1080 1200 1591 1612 1627
Size: 532 471 65 12 120 391 21 15
Cuts at: 1627 1929 2160
Size: 302 231
Fragments arranged by size:
532 471 391 302 231 120 65 21
15 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 625 2160
Size: 359 266 1535
Fragments arranged by size:
1535 359 266
BsiEI CG_ry'CG
Cuts at: 0 1210 2160
Size: 1210 950
BsiHKAI G_wGCw'C
Cuts at: 0 176 214 596 1292 1816 1862 1885 2108
Size: 176 38 382 696 524 46 23 223
Cuts at: 2108 2160
Size: 52
Fragments arranged by size:
696 524 382 223 176 52 46 38
23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 713 2160
Size: 713 1447
Bsp1286I G_dGCh'C
Cuts at: 0 176 214 505 596 916 951 1046 1109
Size: 176 38 291 91 320 35 95 63
Cuts at: 1109 1264 1292 1530 1816 1862 1885 2108 2160
Size: 155 28 238 286 46 23 223 52
Fragments arranged by size:
320 291 286 238 223 176 155 95
91 63 52 46 38 35 28 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 119 242 380 591 735 1504 1665 1902
Size: 119 123 138 211 144 769 161 237
Cuts at: 1902 2160
Size: 258
Fragments arranged by size:
769 258 237 211 161 144 138 123
119
BsrFI r'CCGG_y
Cuts at: 0 6 1178 1205 2160
Size: 6 1172 27 955
Fragments arranged by size:
1172 955 27 6
BsrGI T'GTAC_A
Cuts at: 0 1427 2160
Size: 1427 733
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1866 1908 2160
Size: 1866 42 252
Fragments arranged by size:
1866 252 42
BstEII G'GTnAC_C
Cuts at: 0 1933 2160
Size: 1933 227
BstXI CCAn_nnnn'nTGG
Cuts at: 0 532 2160
Size: 532 1628
BstYI r'GATC_y
Cuts at: 0 434 515 536 707 848 1464 2160
Size: 434 81 21 171 141 616 696
Fragments arranged by size:
696 616 434 171 141 81 21
Bsu36I CC'TnA_GG
Cuts at: 0 511 884 2160
Size: 511 373 1276
Fragments arranged by size:
1276 511 373
BtgI C'CryG_G
Cuts at: 0 1641 1684 2160
Size: 1641 43 476
Fragments arranged by size:
1641 476 43
BtsI GCAGTG_nn'
Cuts at: 0 1722 2160
Size: 1722 438
DraIII CAC_nnn'GTG
Cuts at: 0 663 1866 2160
Size: 663 1203 294
Fragments arranged by size:
1203 663 294
DrdI GACnn_nn'nnGTC
Cuts at: 0 727 2160
Size: 727 1433
EaeI y'GGCC_r
Cuts at: 0 1207 2160
Size: 1207 953
EagI C'GGCC_G
Cuts at: 0 1207 2160
Size: 1207 953
EarI CTCTTCn'nnn_
Cuts at: 0 382 403 1715 2096 2160
Size: 382 21 1312 381 64
Fragments arranged by size:
1312 382 381 64 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1824 1951 2160
Size: 1824 127 209
Fragments arranged by size:
1824 209 127
EcoICRI GAG'CTC
Cuts at: 0 174 212 1814 2160
Size: 174 38 1602 346
Fragments arranged by size:
1602 346 174 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 184 198 737 862 977 1085 1502
Size: 100 84 14 539 125 115 108 417
Cuts at: 1502 2051 2120 2160
Size: 549 69 40
Fragments arranged by size:
549 539 417 125 115 108 100 84
69 40 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 1795 2160
Size: 247 1548 365
Fragments arranged by size:
1548 365 247
EcoO109I rG'GnC_Cy
Cuts at: 0 204 888 947 948 1344 1527 1575 1595
Size: 204 684 59 1 396 183 48 20
Cuts at: 1595 1914 2001 2009 2160
Size: 319 87 8 151
Fragments arranged by size:
684 396 319 204 183 151 87 59
48 20 8 1
FseI GG_CCGG'CC
Cuts at: 0 1209 2160
Size: 1209 951
HaeII r_GCGC'y
Cuts at: 0 1275 1876 2160
Size: 1275 601 284
Fragments arranged by size:
1275 601 284
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 692 724 867 899 1012 1044 1289 1321
Size: 692 32 143 32 113 32 245 32
Cuts at: 1321 1579 1611 2160
Size: 258 32 549
Fragments arranged by size:
692 549 258 245 143 113 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 1271 2160
Size: 1271 889
KpnI G_GTAC'C
Cuts at: 0 396 1317 2160
Size: 396 921 843
Fragments arranged by size:
921 843 396
MfeI C'AATT_G
Cuts at: 0 648 2160
Size: 648 1512
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1120 1385 2160
Size: 428 692 265 775
Fragments arranged by size:
775 692 428 265
MslI CAynn'nnrTG
Cuts at: 0 410 476 1477 2160
Size: 410 66 1001 683
Fragments arranged by size:
1001 683 410 66
MspA1I CmG'CkG
Cuts at: 0 96 343 487 609 1280 2160
Size: 96 247 144 122 671 880
Fragments arranged by size:
880 671 247 144 122 96
NaeI GCC'GGC
Cuts at: 0 1180 1207 2160
Size: 1180 27 953
Fragments arranged by size:
1180 953 27
NarI GG'CG_CC
Cuts at: 0 1272 2160
Size: 1272 888
NcoI C'CATG_G
Cuts at: 0 1641 2160
Size: 1641 519
NgoMIV G'CCGG_C
Cuts at: 0 1178 1205 2160
Size: 1178 27 955
Fragments arranged by size:
1178 955 27
NspI r_CATG'y
Cuts at: 0 428 1476 2160
Size: 428 1048 684
Fragments arranged by size:
1048 684 428
PasI CC'CwG_GG
Cuts at: 0 944 2160
Size: 944 1216
PfoI T'CCnGG_A
Cuts at: 0 890 996 2160
Size: 890 106 1164
Fragments arranged by size:
1164 890 106
PmlI CAC'GTG
Cuts at: 0 528 2160
Size: 528 1632
PpuMI rG'GwC_Cy
Cuts at: 0 888 1575 1595 1914 2160
Size: 888 687 20 319 246
Fragments arranged by size:
888 687 319 246 20
PshAI GACnn'nnGTC
Cuts at: 0 1127 2160
Size: 1127 1033
PspOMI G'GGCC_C
Cuts at: 0 947 1260 1526 2160
Size: 947 313 266 634
Fragments arranged by size:
947 634 313 266
PspXI vC'TCGA_Gb
Cuts at: 0 136 595 2160
Size: 136 459 1565
Fragments arranged by size:
1565 459 136
PstI C_TGCA'G
Cuts at: 0 71 523 707 1662 1904 1913 2160
Size: 71 452 184 955 242 9 247
Fragments arranged by size:
955 452 247 242 184 71 9
PvuII CAG'CTG
Cuts at: 0 96 343 609 2160
Size: 96 247 266 1551
Fragments arranged by size:
1551 266 247 96
SacI G_AGCT'C
Cuts at: 0 176 214 1816 2160
Size: 176 38 1602 344
Fragments arranged by size:
1602 344 176 38
SanDI GG'GwC_CC
Cuts at: 0 1595 2160
Size: 1595 565
SapI GCTCTTCn'nnn_
Cuts at: 0 2096 2160
Size: 2096 64
SbfI CC_TGCA'GG
Cuts at: 0 71 2160
Size: 71 2089
SfcI C'TryA_G
Cuts at: 0 67 220 519 703 1365 1554 1658 1876
Size: 67 153 299 184 662 189 104 218
Cuts at: 1876 1900 1909 2160
Size: 24 9 251
Fragments arranged by size:
662 299 251 218 189 184 153 104
67 24 9
SfoI GGC'GCC
Cuts at: 0 1273 2160
Size: 1273 887
SgrAI Cr'CCGG_yG
Cuts at: 0 1178 2160
Size: 1178 982
SmlI C'TyrA_G
Cuts at: 0 136 595 1291 1622 1888 2160
Size: 136 459 696 331 266 272
Fragments arranged by size:
696 459 331 272 266 136
SphI G_CATG'C
Cuts at: 0 428 2160
Size: 428 1732
StuI AGG'CCT
Cuts at: 0 1157 2160
Size: 1157 1003
StyI C'CwwG_G
Cuts at: 0 291 566 1032 1641 1773 2160
Size: 291 275 466 609 132 387
Fragments arranged by size:
609 466 387 291 275 132
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 694 1432 1467 1579 1921 2160
Size: 694 738 35 112 342 239
Fragments arranged by size:
738 694 342 239 112 35
TatI w'GTAC_w
Cuts at: 0 1427 1991 2160
Size: 1427 564 169
Fragments arranged by size:
1427 564 169
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 649 669 681 701 2160
Size: 649 20 12 20 1459
Fragments arranged by size:
1459 649 20 20 12
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 573 1368 2160
Size: 573 795 792
Fragments arranged by size:
795 792 573
XhoI C'TCGA_G
Cuts at: 0 136 595 2160
Size: 136 459 1565
Fragments arranged by size:
1565 459 136
ZraI GAC'GTC
Cuts at: 0 963 2160
Size: 963 1197
Enzymes that do cut:
AarI AatII Acc65I AcuI AgeI AlfI AlwNI ApaI
ApaLI ApoI AvaI AvrII BanI BanII BbeI BbsI
BbvCI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrFI
BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I FseI HaeII Hin4I KasI KpnI MfeI
MmeI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PfoI PmlI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SanDI SapI SbfI SfcI SfoI SgrAI
SmlI SphI StuI StyI TaqII TatI TstI XcmI
XhoI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AhdI AleI AloI
AscI AseI AsiSI BaeI BamHI BcgI BciVI BfrBI
BglI BmgBI BmtI BsaBI BsiWI BsmI BspEI BspHI
BsrBI BsrDI BssHII BssSI BstBI BstZ17I BtgZI ClaI
CspCI DraI EcoRI EcoRV FalI FspI FspAI HincII
HindIII HpaI MluI MscI NdeI NheI NotI NruI
NsiI PacI PciI PflMI PmeI PpiI PsiI PsrI
PvuI RsrII SacII SalI ScaI SexAI SfiI SgfI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI
XmaI XmnI