Restriction map for
pFN21AB5686
(Linear) (Six-base) MAPSORT of: KIBB5686.seq Check: 2340 from: 1 to: 1440
KIBB5686.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AfeI AGC'GCT
Cuts at: 0 176 712 1440
Size: 176 536 728
Fragments arranged by size:
728 536 176
AflII C'TTAA_G
Cuts at: 0 1420 1440
Size: 1420 20
AflIII A'CryG_T
Cuts at: 0 701 703 820 1440
Size: 701 2 117 620
Fragments arranged by size:
701 620 117 2
AhdI GACnn_n'nnGTC
Cuts at: 0 196 1440
Size: 196 1244
AleI CACnn'nnGTG
Cuts at: 0 254 745 764 1440
Size: 254 491 19 676
Fragments arranged by size:
676 491 254 19
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 479 763 1440
Size: 479 284 677
Fragments arranged by size:
677 479 284
AlwNI CAG_nnn'CTG
Cuts at: 0 1101 1266 1440
Size: 1101 165 174
Fragments arranged by size:
1101 174 165
ApaI G_GGCC'C
Cuts at: 0 81 1440
Size: 81 1359
ApoI r'AATT_y
Cuts at: 0 977 1440
Size: 977 463
AvaI C'yCGr_G
Cuts at: 0 170 544 1440
Size: 170 374 896
Fragments arranged by size:
896 374 170
BanI G'GyrC_C
Cuts at: 0 739 1440
Size: 739 701
BanII G_rGCy'C
Cuts at: 0 81 737 1095 1440
Size: 81 656 358 345
Fragments arranged by size:
656 358 345 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 140 1440
Size: 140 1300
BclI T'GATC_A
Cuts at: 0 615 1440
Size: 615 825
BglI GCCn_nnn'nGGC
Cuts at: 0 265 1440
Size: 265 1175
BglII A'GATC_T
Cuts at: 0 563 1440
Size: 563 877
BlpI GC'TnA_GC
Cuts at: 0 115 127 1440
Size: 115 12 1313
Fragments arranged by size:
1313 115 12
Bme1580I G_kGCm'C
Cuts at: 0 81 261 1440
Size: 81 180 1179
Fragments arranged by size:
1179 180 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 420 1440
Size: 420 1020
Bpu10I CC'TnA_GC
Cuts at: 0 1064 1273 1440
Size: 1064 209 167
Fragments arranged by size:
1064 209 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 183 1199 1440
Size: 183 1016 241
Fragments arranged by size:
1016 241 183
BsaAI yAC'GTr
Cuts at: 0 704 821 1440
Size: 704 117 619
Fragments arranged by size:
704 619 117
BsaBI GATnn'nnATC
Cuts at: 0 281 1440
Size: 281 1159
BsaHI Gr'CG_yC
Cuts at: 0 1319 1440
Size: 1319 121
BsaWI w'CCGG_w
Cuts at: 0 1016 1440
Size: 1016 424
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 819 849 1024 1054 1440
Size: 819 30 175 30 386
Fragments arranged by size:
819 386 175 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 562 1414 1440
Size: 562 852 26
Fragments arranged by size:
852 562 26
BseYI C'CCAG_C
Cuts at: 0 407 506 1387 1440
Size: 407 99 881 53
Fragments arranged by size:
881 407 99 53
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1018 1440
Size: 769 249 422
Fragments arranged by size:
769 422 249
BsiEI CG_ry'CG
Cuts at: 0 85 1440
Size: 85 1355
BsiHKAI G_wGCw'C
Cuts at: 0 16 1440
Size: 16 1424
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1102 1440
Size: 1102 338
Bsp1286I G_dGCh'C
Cuts at: 0 16 81 261 737 1095 1440
Size: 16 65 180 476 358 345
Fragments arranged by size:
476 358 345 180 65 16
BspEI T'CCGG_A
Cuts at: 0 1016 1440
Size: 1016 424
BsrDI GCAATG_nn'
Cuts at: 0 753 1440
Size: 753 687
BsrFI r'CCGG_y
Cuts at: 0 57 1253 1440
Size: 57 1196 187
Fragments arranged by size:
1196 187 57
BssSI C'ACGA_G
Cuts at: 0 252 1440
Size: 252 1188
BstXI CCAn_nnnn'nTGG
Cuts at: 0 766 1440
Size: 766 674
BstYI r'GATC_y
Cuts at: 0 563 1440
Size: 563 877
Bsu36I CC'TnA_GG
Cuts at: 0 623 1440
Size: 623 817
BtgI C'CryG_G
Cuts at: 0 49 840 1349 1440
Size: 49 791 509 91
Fragments arranged by size:
791 509 91 49
EaeI y'GGCC_r
Cuts at: 0 82 266 837 942 1440
Size: 82 184 571 105 498
Fragments arranged by size:
571 498 184 105 82
EagI C'GGCC_G
Cuts at: 0 82 1440
Size: 82 1358
EarI CTCTTCn'nnn_
Cuts at: 0 442 591 1076 1440
Size: 442 149 485 364
Fragments arranged by size:
485 442 364 149
EcoO109I rG'GnC_Cy
Cuts at: 0 44 77 529 799 1098 1440
Size: 44 33 452 270 299 342
Fragments arranged by size:
452 342 299 270 44 33
HaeII r_GCGC'y
Cuts at: 0 178 714 1440
Size: 178 536 726
Fragments arranged by size:
726 536 178
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 182 214 565 597 636 668 899 931
Size: 182 32 351 32 39 32 231 32
Cuts at: 931 1024 1056 1440
Size: 93 32 384
Fragments arranged by size:
384 351 231 182 93 39 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 644 672 1440
Size: 644 28 768
Fragments arranged by size:
768 644 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1067 1440
Size: 1067 373
MscI TGG'CCA
Cuts at: 0 268 944 1440
Size: 268 676 496
Fragments arranged by size:
676 496 268
MslI CAynn'nnrTG
Cuts at: 0 254 745 764 1440
Size: 254 491 19 676
Fragments arranged by size:
676 491 254 19
MspA1I CmG'CkG
Cuts at: 0 51 411 500 842 1440
Size: 51 360 89 342 598
Fragments arranged by size:
598 360 342 89 51
NaeI GCC'GGC
Cuts at: 0 59 1255 1440
Size: 59 1196 185
Fragments arranged by size:
1196 185 59
NcoI C'CATG_G
Cuts at: 0 1349 1440
Size: 1349 91
NgoMIV G'CCGG_C
Cuts at: 0 57 1253 1440
Size: 57 1196 187
Fragments arranged by size:
1196 187 57
PasI CC'CwG_GG
Cuts at: 0 89 1095 1132 1440
Size: 89 1006 37 308
Fragments arranged by size:
1006 308 89 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 23 1440
Size: 23 1417
PmlI CAC'GTG
Cuts at: 0 704 821 1440
Size: 704 117 619
Fragments arranged by size:
704 619 117
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 538 570 1440
Size: 538 32 870
Fragments arranged by size:
870 538 32
PpuMI rG'GwC_Cy
Cuts at: 0 44 1440
Size: 44 1396
PspOMI G'GGCC_C
Cuts at: 0 77 1440
Size: 77 1363
PstI C_TGCA'G
Cuts at: 0 480 877 930 1440
Size: 480 397 53 510
Fragments arranged by size:
510 480 397 53
PvuII CAG'CTG
Cuts at: 0 411 1440
Size: 411 1029
SacII CC_GC'GG
Cuts at: 0 52 843 1440
Size: 52 791 597
Fragments arranged by size:
791 597 52
SanDI GG'GwC_CC
Cuts at: 0 44 1440
Size: 44 1396
SapI GCTCTTCn'nnn_
Cuts at: 0 1076 1440
Size: 1076 364
SbfI CC_TGCA'GG
Cuts at: 0 930 1440
Size: 930 510
SexAI A'CCwGG_T
Cuts at: 0 192 1440
Size: 192 1248
SfcI C'TryA_G
Cuts at: 0 476 873 926 1058 1440
Size: 476 397 53 132 382
Fragments arranged by size:
476 397 382 132 53
SmlI C'TyrA_G
Cuts at: 0 1420 1440
Size: 1420 20
StyI C'CwwG_G
Cuts at: 0 392 743 1349 1440
Size: 392 351 606 91
Fragments arranged by size:
606 392 351 91
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1153 1440
Size: 1153 287
TatI w'GTAC_w
Cuts at: 0 235 1440
Size: 235 1205
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 819 851 1440
Size: 819 32 589
Fragments arranged by size:
819 589 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 96 1440
Size: 96 1344
Enzymes that do cut:
AfeI AflII AflIII AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BclI BglI BglII
BlpI Bme1580I BplI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BsrDI BsrFI BssSI BstXI BstYI Bsu36I
BtgI EaeI EagI EarI EcoO109I HaeII Hin4I HincII
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV PasI
PflMI PmlI PpiI PpuMI PspOMI PstI PvuII SacII
SanDI SapI SbfI SexAI SfcI SmlI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BfrBI BmgBI BmrI BmtI BpmI
BpuEI BsiWI BsmI BspHI BspMI BsrBI BsrGI BssHII
BstAPI BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HindIII HpaI KasI
KpnI MfeI MluI NarI NdeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SalI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI