Restriction map for
pFN21AB5624
(Linear) (Six-base) MAPSORT of: KIBB5624.seq Check: 2568 from: 1 to: 6858
KIBB5624.orf
With 182 enzymes: SgfI *
August 3, 2016 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 4930 5054 6858
Size: 4930 124 1804
Fragments arranged by size:
4930 1804 124
AccI GT'mk_AC
Cuts at: 0 3151 3530 4181 6858
Size: 3151 379 651 2677
Fragments arranged by size:
3151 2677 651 379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 727 1849 4702 4798 5176 5358 5404
Size: 178 549 1122 2853 96 378 182 46
Cuts at: 5404 6685 6858
Size: 1281 173
Fragments arranged by size:
2853 1281 1122 549 378 182 178 173
96 46
AfeI AGC'GCT
Cuts at: 0 229 2377 4493 6858
Size: 229 2148 2116 2365
Fragments arranged by size:
2365 2148 2116 229
AflIII A'CryG_T
Cuts at: 0 554 1047 1328 1593 3477 4214 6320 6858
Size: 554 493 281 265 1884 737 2106 538
Fragments arranged by size:
2106 1884 737 554 538 493 281 265
AleI CACnn'nnGTG
Cuts at: 0 2212 6070 6858
Size: 2212 3858 788
Fragments arranged by size:
3858 2212 788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1943 4078 4582 5988 6858
Size: 1943 2135 504 1406 870
Fragments arranged by size:
2135 1943 1406 870 504
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6412 6444 6858
Size: 6412 32 414
Fragments arranged by size:
6412 414 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1362 1387 2736 3676 3882 4675 5199 6654
Size: 1362 25 1349 940 206 793 524 1455
Cuts at: 6654 6858
Size: 204
Fragments arranged by size:
1455 1362 1349 940 793 524 206 204
25
ApaI G_GGCC'C
Cuts at: 0 2807 4311 4627 4745 6858
Size: 2807 1504 316 118 2113
Fragments arranged by size:
2807 2113 1504 316 118
ApaLI G'TGCA_C
Cuts at: 0 1443 4315 6858
Size: 1443 2872 2543
Fragments arranged by size:
2872 2543 1443
ApoI r'AATT_y
Cuts at: 0 2622 4796 4813 5734 6858
Size: 2622 2174 17 921 1124
Fragments arranged by size:
2622 2174 1124 921 17
AvaI C'yCGr_G
Cuts at: 0 1121 1639 2111 3250 3910 6858
Size: 1121 518 472 1139 660 2948
Fragments arranged by size:
2948 1139 1121 660 518 472
BamHI G'GATC_C
Cuts at: 0 2054 5378 5751 6858
Size: 2054 3324 373 1107
Fragments arranged by size:
3324 2054 1107 373
BanI G'GyrC_C
Cuts at: 0 70 1194 2680 4358 4579 4824 6573 6858
Size: 70 1124 1486 1678 221 245 1749 285
Fragments arranged by size:
1749 1678 1486 1124 285 245 221 70
BanII G_rGCy'C
Cuts at: 0 1466 1489 2312 2472 2807 2903 3095 3259
Size: 1466 23 823 160 335 96 192 164
Cuts at: 3259 3308 3349 3557 4311 4627 4634 4745 4887
Size: 49 41 208 754 316 7 111 142
Cuts at: 4887 6129 6176 6248 6858
Size: 1242 47 72 610
Fragments arranged by size:
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192 164 160 142 111 96 72 49
47 41 23 7
BbeI G_GCGC'C
Cuts at: 0 74 1198 2684 4583 6577 6858
Size: 74 1124 1486 1899 1994 281
Fragments arranged by size:
1994 1899 1486 1124 281 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 181 993 1732 2622 2888 3611 4690 6741
Size: 181 812 739 890 266 723 1079 2051
Cuts at: 6741 6858
Size: 117
Fragments arranged by size:
2051 1079 890 812 739 723 266 181
117
BbvCI CC'TCA_GC
Cuts at: 0 2366 6043 6858
Size: 2366 3677 815
Fragments arranged by size:
3677 2366 815
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 822 856 6422 6456 6571 6605 6858
Size: 822 34 5566 34 115 34 253
Fragments arranged by size:
5566 822 253 115 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 4459 6858
Size: 4459 2399
BclI T'GATC_A
Cuts at: 0 5306 6140 6858
Size: 5306 834 718
Fragments arranged by size:
5306 834 718
BfrBI ATG'CAT
Cuts at: 0 198 6858
Size: 198 6660
BglI GCCn_nnn'nGGC
Cuts at: 0 1285 3025 4025 6115 6552 6858
Size: 1285 1740 1000 2090 437 306
Fragments arranged by size:
2090 1740 1285 1000 437 306
BglII A'GATC_T
Cuts at: 0 506 2948 6858
Size: 506 2442 3910
Fragments arranged by size:
3910 2442 506
BlpI GC'TnA_GC
Cuts at: 0 2741 2897 4078 4247 6858
Size: 2741 156 1181 169 2611
Fragments arranged by size:
2741 2611 1181 169 156
Bme1580I G_kGCm'C
Cuts at: 0 1447 2807 3749 4311 4319 4627 4745 5334
Size: 1447 1360 942 562 8 308 118 589
Cuts at: 5334 6858
Size: 1524
Fragments arranged by size:
1524 1447 1360 942 589 562 308 118
8
BmgBI CAC'GTC
Cuts at: 0 1050 1878 3045 5277 6858
Size: 1050 828 1167 2232 1581
Fragments arranged by size:
2232 1581 1167 1050 828
BmrI ACTGGGnnnn_n'
Cuts at: 0 1194 3463 4416 5415 6420 6858
Size: 1194 2269 953 999 1005 438
Fragments arranged by size:
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BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 636 2766 3114 4428 5007 6858
Size: 636 2130 348 1314 579 1851
Fragments arranged by size:
2130 1851 1314 636 579 348
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1997 2089 3535 3586 3904 4423 4633 5014
Size: 1997 92 1446 51 318 519 210 381
Cuts at: 5014 5203 5317 6041 6325 6832 6841 6858
Size: 189 114 724 284 507 9 17
Fragments arranged by size:
1997 1446 724 519 507 381 318 284
210 189 114 92 51 17 9
Bpu10I CC'TnA_GC
Cuts at: 0 231 2366 3904 4628 6043 6858
Size: 231 2135 1538 724 1415 815
Fragments arranged by size:
2135 1538 1415 815 724 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 832 1255 1765 2887 2965 3325 4414
Size: 79 753 423 510 1122 78 360 1089
Cuts at: 4414 6858
Size: 2444
Fragments arranged by size:
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78
BsaI GGTCTCn'nnnn_
Cuts at: 0 1051 1603 3188 4006 4375 5686 6374 6428
Size: 1051 552 1585 818 369 1311 688 54
Cuts at: 6428 6679 6808 6858
Size: 251 129 50
Fragments arranged by size:
1585 1311 1051 818 688 552 369 251
129 54 50
BsaAI yAC'GTr
Cuts at: 0 717 1370 1596 1629 2262 3478 4101 5658
Size: 717 653 226 33 633 1216 623 1557
Cuts at: 5658 6321 6453 6858
Size: 663 132 405
Fragments arranged by size:
1557 1216 717 663 653 633 623 405
226 132 33
BsaBI GATnn'nnATC
Cuts at: 0 2203 2947 4507 6858
Size: 2203 744 1560 2351
Fragments arranged by size:
2351 2203 1560 744
BsaHI Gr'CG_yC
Cuts at: 0 71 1195 2681 4580 6574 6858
Size: 71 1124 1486 1899 1994 284
Fragments arranged by size:
1994 1899 1486 1124 284 71
BsaWI w'CCGG_w
Cuts at: 0 759 3329 3815 6773 6858
Size: 759 2570 486 2958 85
Fragments arranged by size:
2958 2570 759 486 85
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1315 1345 2383 2413 3133 3163 3223 3253
Size: 1315 30 1038 30 720 30 60 30
Cuts at: 3253 6412 6442 6858
Size: 3159 30 416
Fragments arranged by size:
3159 1315 1038 720 416 60 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 24 1768 3733 4141 4597 4744 5881 5899
Size: 24 1744 1965 408 456 147 1137 18
Cuts at: 5899 5902 6090 6093 6433 6576 6858
Size: 3 188 3 340 143 282
Fragments arranged by size:
1965 1744 1137 456 408 340 282 188
147 143 24 18 3 3
BseYI C'CCAG_C
Cuts at: 0 1840 3408 3666 3942 4370 4570 4769 5402
Size: 1840 1568 258 276 428 200 199 633
Cuts at: 5402 5729 6785 6849 6858
Size: 327 1056 64 9
Fragments arranged by size:
1840 1568 1056 633 428 327 276 258
200 199 64 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1540 3484 4021 5494 6858
Size: 260 1280 1944 537 1473 1364
Fragments arranged by size:
1944 1473 1364 1280 537 260
BsiEI CG_ry'CG
Cuts at: 0 1280 2345 5479 6122 6858
Size: 1280 1065 3134 643 736
Fragments arranged by size:
3134 1280 1065 736 643
BsiHKAI G_wGCw'C
Cuts at: 0 1447 2903 3095 3349 3557 3627 3905 4319
Size: 1447 1456 192 254 208 70 278 414
Cuts at: 4319 4400 4601 4634 5365 6057 6067 6176 6248
Size: 81 201 33 731 692 10 109 72
Cuts at: 6248 6858
Size: 610
Fragments arranged by size:
1456 1447 731 692 610 414 278 254
208 201 192 109 81 72 70 33
10
BsmBI CGTCTCn'nnnn_
Cuts at: 0 823 2230 6858
Size: 823 1407 4628
Fragments arranged by size:
4628 1407 823
Bsp1286I G_dGCh'C
Cuts at: 0 1447 1466 1489 2312 2472 2807 2903 3095
Size: 1447 19 23 823 160 335 96 192
Cuts at: 3095 3259 3308 3349 3557 3627 3749 3905 4311
Size: 164 49 41 208 70 122 156 406
Cuts at: 4311 4319 4400 4601 4627 4634 4745 4887 5334
Size: 8 81 201 26 7 111 142 447
Cuts at: 5334 5365 6057 6067 6129 6176 6248 6858
Size: 31 692 10 62 47 72 610
Fragments arranged by size:
1447 823 692 610 447 406 335 208
201 192 164 160 156 142 122 111
96 81 72 70 62 49 47 41
31 26 23 19 10 8 7
BspEI T'CCGG_A
Cuts at: 0 3329 6773 6858
Size: 3329 3444 85
Fragments arranged by size:
3444 3329 85
BspHI T'CATG_A
Cuts at: 0 2121 5496 5990 6858
Size: 2121 3375 494 868
Fragments arranged by size:
3375 2121 868 494
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3447 4930 4940 5054 6392 6858
Size: 3447 1483 10 114 1338 466
Fragments arranged by size:
3447 1483 1338 466 114 10
BsrBI CCG'CTC
Cuts at: 0 14 153 2771 3405 6446 6858
Size: 14 139 2618 634 3041 412
Fragments arranged by size:
3041 2618 634 412 139 14
BsrDI GCAATG_nn'
Cuts at: 0 314 6779 6858
Size: 314 6465 79
Fragments arranged by size:
6465 314 79
BsrFI r'CCGG_y
Cuts at: 0 1673 4024 6858
Size: 1673 2351 2834
Fragments arranged by size:
2834 2351 1673
BsrGI T'GTAC_A
Cuts at: 0 1406 3050 5620 6858
Size: 1406 1644 2570 1238
Fragments arranged by size:
2570 1644 1406 1238
BssHII G'CGCG_C
Cuts at: 0 27 6858
Size: 27 6831
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2504 3999 5955 6858
Size: 2504 1495 1956 903
Fragments arranged by size:
2504 1956 1495 903
BstBI TT'CG_AA
Cuts at: 0 4658 6858
Size: 4658 2200
BstEII G'GTnAC_C
Cuts at: 0 1077 6858
Size: 1077 5781
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1074 2490 3942 6858
Size: 1074 1416 1452 2916
Fragments arranged by size:
2916 1452 1416 1074
BstYI r'GATC_y
Cuts at: 0 411 483 506 710 2054 2198 2948 4502
Size: 411 72 23 204 1344 144 750 1554
Cuts at: 4502 5378 5751 6858
Size: 876 373 1107
Fragments arranged by size:
1554 1344 1107 876 750 411 373 204
144 72 23
Bsu36I CC'TnA_GG
Cuts at: 0 1222 6461 6858
Size: 1222 5239 397
Fragments arranged by size:
5239 1222 397
BtgI C'CryG_G
Cuts at: 0 1134 1307 1343 1457 1931 2345 2483 2925
Size: 1134 173 36 114 474 414 138 442
Cuts at: 2925 3308 3935 3988 4878 6672 6858
Size: 383 627 53 890 1794 186
Fragments arranged by size:
1794 1134 890 627 474 442 414 383
186 173 138 114 53 36
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 4737 5541 5634 6858
Size: 4737 804 93 1224
Fragments arranged by size:
4737 1224 804 93
BtsI GCAGTG_nn'
Cuts at: 0 243 248 2014 4062 4065 6024 6057 6858
Size: 243 5 1766 2048 3 1959 33 801
Fragments arranged by size:
2048 1959 1766 801 243 33 5 3
ClaI AT'CG_AT
Cuts at: 0 899 6858
Size: 899 5959
DraIII CAC_nnn'GTG
Cuts at: 0 435 477 1499 2319 5174 6858
Size: 435 42 1022 820 2855 1684
Fragments arranged by size:
2855 1684 1022 820 435 42
DrdI GACnn_nn'nnGTC
Cuts at: 0 3202 6858
Size: 3202 3656
EaeI y'GGCC_r
Cuts at: 0 903 1105 1277 1340 1364 2342 3179 3590
Size: 903 202 172 63 24 978 837 411
Cuts at: 3590 4787 5201 5336 6014 6858
Size: 1197 414 135 678 844
Fragments arranged by size:
1197 978 903 844 837 678 414 411
202 172 135 63 24
EagI C'GGCC_G
Cuts at: 0 1277 2342 6858
Size: 1277 1065 4516
Fragments arranged by size:
4516 1277 1065
EarI CTCTTCn'nnn_
Cuts at: 0 605 1228 1745 1784 2152 2213 2854 2993
Size: 605 623 517 39 368 61 641 139
Cuts at: 2993 3354 3562 3682 3865 4501 4774 5017 5380
Size: 361 208 120 183 636 273 243 363
Cuts at: 5380 5548 5888 6522 6858
Size: 168 340 634 336
Fragments arranged by size:
641 636 634 623 605 517 368 363
361 340 336 273 243 208 183 168
139 120 61 39
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 679 2185 4441 5783 5914 6858
Size: 679 1506 2256 1342 131 944
Fragments arranged by size:
2256 1506 1342 944 679 131
EcoICRI GAG'CTC
Cuts at: 0 2901 3093 3347 3555 4632 6174 6246 6858
Size: 2901 192 254 208 1077 1542 72 612
Fragments arranged by size:
2901 1542 1077 612 254 208 192 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 727 1849 1997 2089 3535 3586 3904
Size: 178 549 1122 148 92 1446 51 318
Cuts at: 3904 4423 4633 4702 4798 5014 5176 5203 5317
Size: 519 210 69 96 216 162 27 114
Cuts at: 5317 5358 5404 6041 6325 6685 6832 6841 6858
Size: 41 46 637 284 360 147 9 17
Fragments arranged by size:
1446 1122 637 549 519 360 318 284
216 210 178 162 148 147 114 96
92 69 51 46 41 27 17 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3454 4615 4930 4981 6858
Size: 3454 1161 315 51 1877
Fragments arranged by size:
3454 1877 1161 315 51
EcoO109I rG'GnC_Cy
Cuts at: 0 988 1635 2564 2804 3729 4158 4308 4624
Size: 988 647 929 240 925 429 150 316
Cuts at: 4624 4741 4860 6651 6858
Size: 117 119 1791 207
Fragments arranged by size:
1791 988 929 925 647 429 316 240
207 150 119 117
EcoRI G'AATT_C
Cuts at: 0 4813 6858
Size: 4813 2045
EcoRV GAT'ATC
Cuts at: 0 1665 6858
Size: 1665 5193
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2727 5559 5712 6858
Size: 2727 2832 153 1146
Fragments arranged by size:
2832 2727 1146 153
FseI GG_CCGG'CC
Cuts at: 0 4028 6858
Size: 4028 2830
FspI TGC'GCA
Cuts at: 0 2461 4555 6858
Size: 2461 2094 2303
Fragments arranged by size:
2461 2303 2094
HaeII r_GCGC'y
Cuts at: 0 74 102 231 1198 2379 2684 3177 3419
Size: 74 28 129 967 1181 305 493 242
Cuts at: 3419 3858 4048 4261 4495 4583 4737 4878 5037
Size: 439 190 213 234 88 154 141 159
Cuts at: 5037 5574 5634 6577 6858
Size: 537 60 943 281
Fragments arranged by size:
1181 967 943 537 493 439 305 281
242 234 213 190 159 154 141 129
88 74 60 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3133 3165 3864 3896 4374 4398 4406 4430
Size: 3133 32 699 32 478 24 8 24
Cuts at: 4430 4700 4732 6858
Size: 270 32 2126
Fragments arranged by size:
3133 2126 699 478 270 32 32 32
24 24 8
HincII GTy'rAC
Cuts at: 0 5208 5235 6858
Size: 5208 27 1623
Fragments arranged by size:
5208 1623 27
HindIII A'AGCT_T
Cuts at: 0 1816 6858
Size: 1816 5042
KasI G'GCGC_C
Cuts at: 0 70 1194 2680 4579 6573 6858
Size: 70 1124 1486 1899 1994 285
Fragments arranged by size:
1994 1899 1486 1124 285 70
MluI A'CGCG_T
Cuts at: 0 554 6858
Size: 554 6304
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 4196 5030 5933 6102 6587 6858
Size: 623 3573 834 903 169 485 271
Fragments arranged by size:
3573 903 834 623 485 271 169
MscI TGG'CCA
Cuts at: 0 905 1107 1342 1366 4789 6858
Size: 905 202 235 24 3423 2069
Fragments arranged by size:
3423 2069 905 235 202 24
MslI CAynn'nnrTG
Cuts at: 0 193 1072 1210 2212 2392 2488 5395 6070
Size: 193 879 138 1002 180 96 2907 675
Cuts at: 6070 6598 6697 6858
Size: 528 99 161
Fragments arranged by size:
2907 1002 879 675 528 193 180 161
138 99 96
MspA1I CmG'CkG
Cuts at: 0 1716 1992 2067 2244 2415 2927 2940 3840
Size: 1716 276 75 177 171 512 13 900
Cuts at: 3840 4350 4769 5468 6479 6674 6710 6849 6858
Size: 510 419 699 1011 195 36 139 9
Fragments arranged by size:
1716 1011 900 699 512 510 419 276
195 177 171 139 75 36 13 9
NaeI GCC'GGC
Cuts at: 0 4026 6858
Size: 4026 2832
NarI GG'CG_CC
Cuts at: 0 71 1195 2681 4580 6574 6858
Size: 71 1124 1486 1899 1994 284
Fragments arranged by size:
1994 1899 1486 1124 284 71
NcoI C'CATG_G
Cuts at: 0 1457 1931 2483 4878 6858
Size: 1457 474 552 2395 1980
Fragments arranged by size:
2395 1980 1457 552 474
NgoMIV G'CCGG_C
Cuts at: 0 4024 6858
Size: 4024 2834
NotI GC'GGCC_GC
Cuts at: 0 1277 6858
Size: 1277 5581
NsiI A_TGCA'T
Cuts at: 0 200 6858
Size: 200 6658
NspI r_CATG'y
Cuts at: 0 198 663 1149 1332 3072 4218 6696 6801
Size: 198 465 486 183 1740 1146 2478 105
Cuts at: 6801 6858
Size: 57
Fragments arranged by size:
2478 1740 1146 486 465 198 183 105
57
PasI CC'CwG_GG
Cuts at: 0 3780 6858
Size: 3780 3078
PciI A'CATG_T
Cuts at: 0 1328 4214 6858
Size: 1328 2886 2644
Fragments arranged by size:
2886 2644 1328
PflMI CCAn_nnn'nTGG
Cuts at: 0 477 1362 2736 6858
Size: 477 885 1374 4122
Fragments arranged by size:
4122 1374 885 477
PfoI T'CCnGG_A
Cuts at: 0 1052 2282 3920 3977 4610 4862 6132 6809
Size: 1052 1230 1638 57 633 252 1270 677
Cuts at: 6809 6858
Size: 49
Fragments arranged by size:
1638 1270 1230 1052 677 633 252 57
49
PmlI CAC'GTG
Cuts at: 0 717 1596 1629 3478 6858
Size: 717 879 33 1849 3380
Fragments arranged by size:
3380 1849 879 717 33
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1552 1584 6412 6444 6858
Size: 1552 32 4828 32 414
Fragments arranged by size:
4828 1552 414 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 3729 4158 4860 6651 6858
Size: 3729 429 702 1791 207
Fragments arranged by size:
3729 1791 702 429 207
PshAI GACnn'nnGTC
Cuts at: 0 1615 5105 6858
Size: 1615 3490 1753
Fragments arranged by size:
3490 1753 1615
PspOMI G'GGCC_C
Cuts at: 0 2803 4307 4623 4741 6858
Size: 2803 1504 316 118 2117
Fragments arranged by size:
2803 2117 1504 316 118
PspXI vC'TCGA_Gb
Cuts at: 0 1639 6858
Size: 1639 5219
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2857 2889 6858
Size: 2857 32 3969
Fragments arranged by size:
3969 2857 32
PstI C_TGCA'G
Cuts at: 0 587 1715 1922 2414 2939 3455 3845 4696
Size: 587 1128 207 492 525 516 390 851
Cuts at: 4696 5552 6650 6709 6836 6858
Size: 856 1098 59 127 22
Fragments arranged by size:
1128 1098 856 851 587 525 516 492
390 207 127 59 22
PvuII CAG'CTG
Cuts at: 0 2067 2415 2940 3840 4350 4769 6479 6710
Size: 2067 348 525 900 510 419 1710 231
Cuts at: 6710 6849 6858
Size: 139 9
Fragments arranged by size:
2067 1710 900 525 510 419 348 231
139 9
SacI G_AGCT'C
Cuts at: 0 2903 3095 3349 3557 4634 6176 6248 6858
Size: 2903 192 254 208 1077 1542 72 610
Fragments arranged by size:
2903 1542 1077 610 254 208 192 72
SacII CC_GC'GG
Cuts at: 0 2928 6675 6858
Size: 2928 3747 183
Fragments arranged by size:
3747 2928 183
SapI GCTCTTCn'nnn_
Cuts at: 0 2213 3354 3562 4501 5380 5548 6858
Size: 2213 1141 208 939 879 168 1310
Fragments arranged by size:
2213 1310 1141 939 879 208 168
SbfI CC_TGCA'GG
Cuts at: 0 6650 6858
Size: 6650 208
SexAI A'CCwGG_T
Cuts at: 0 5447 6359 6653 6858
Size: 5447 912 294 205
Fragments arranged by size:
5447 912 294 205
SfcI C'TryA_G
Cuts at: 0 583 1711 1918 2410 2935 3014 3451 3841
Size: 583 1128 207 492 525 79 437 390
Cuts at: 3841 4692 5063 5548 6619 6646 6705 6832 6858
Size: 851 371 485 1071 27 59 127 26
Fragments arranged by size:
1128 1071 851 583 525 492 485 437
390 371 207 127 79 59 27 26
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1285 6858
Size: 1285 5573
SfoI GGC'GCC
Cuts at: 0 72 1196 2682 4581 6575 6858
Size: 72 1124 1486 1899 1994 283
Fragments arranged by size:
1994 1899 1486 1124 283 72
SmaI CCC'GGG
Cuts at: 0 1123 3912 6858
Size: 1123 2789 2946
Fragments arranged by size:
2946 2789 1123
SmlI C'TyrA_G
Cuts at: 0 94 847 1270 1639 1744 2902 2980 3340
Size: 94 753 423 369 105 1158 78 360
Cuts at: 3340 4393 6858
Size: 1053 2465
Fragments arranged by size:
2465 1158 1053 753 423 369 360 105
94 78
SphI G_CATG'C
Cuts at: 0 1149 6696 6858
Size: 1149 5547 162
Fragments arranged by size:
5547 1149 162
SrfI GCCC'GGGC
Cuts at: 0 1123 6858
Size: 1123 5735
SspI AAT'ATT
Cuts at: 0 6770 6858
Size: 6770 88
StuI AGG'CCT
Cuts at: 0 1507 1525 2974 3598 4936 5290 6858
Size: 1507 18 1449 624 1338 354 1568
Fragments arranged by size:
1568 1507 1449 1338 624 354 18
StyI C'CwwG_G
Cuts at: 0 1283 1457 1931 2210 2483 2834 3020 4575
Size: 1283 174 474 279 273 351 186 1555
Cuts at: 4575 4878 6858
Size: 303 1980
Fragments arranged by size:
1980 1555 1283 474 351 303 279 273
186 174
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1218 1439 2545 3680 5071 5394 5434 5465
Size: 1218 221 1106 1135 1391 323 40 31
Cuts at: 5465 6757 6858
Size: 1292 101
Fragments arranged by size:
1391 1292 1218 1135 1106 323 221 101
40 31
TatI w'GTAC_w
Cuts at: 0 1187 1406 3050 4586 5620 5663 6701 6858
Size: 1187 219 1644 1536 1034 43 1038 157
Fragments arranged by size:
1644 1536 1187 1038 1034 219 157 43
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1315 1347 1437 1469 3973 4005 6858
Size: 1315 32 90 32 2504 32 2853
Fragments arranged by size:
2853 2504 1315 90 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 6655 6858
Size: 6655 203
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 1455 5175 5655 6858
Size: 411 1044 3720 480 1203
Fragments arranged by size:
3720 1203 1044 480 411
XhoI C'TCGA_G
Cuts at: 0 1639 6858
Size: 1639 5219
XmaI C'CCGG_G
Cuts at: 0 1121 3910 6858
Size: 1121 2789 2948
Fragments arranged by size:
2948 2789 1121
XmnI GAAnn'nnTTC
Cuts at: 0 2440 5542 5738 5784 5983 6858
Size: 2440 3102 196 46 199 875
Fragments arranged by size:
3102 2440 875 199 196 46
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI HaeII
Hin4I HincII HindIII KasI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NsiI NspI
PasI PciI PflMI PfoI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PstI PvuII SacI SacII SapI
SbfI SexAI SfcI SfiI SfoI SmaI SmlI SphI
SrfI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AgeI AhdI AscI AseI
AsiSI AvrII BaeI BmtI BsiWI BsmI BssSI BstZ17I
CspCI DraI FspAI HpaI KpnI MfeI NdeI NheI
NruI PacI PmeI PsiI PvuI RsrII SalI SanDI
ScaI SgfI SgrAI SnaBI SpeI SwaI XbaI ZraI