Restriction map for
pFN21AB5617
(Linear) (Six-base) MAPSORT of: KIBB5617.seq Check: 6523 from: 1 to: 4224
KIBB5617.orf
With 182 enzymes: SgfI *
April 1, 2016 14:38 ..
AccI GT'mk_AC
Cuts at: 0 365 3056 4224
Size: 365 2691 1168
Fragments arranged by size:
2691 1168 365
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 934 2190 2675 2741 4224
Size: 217 717 1256 485 66 1483
Fragments arranged by size:
1483 1256 717 485 217 66
AflIII A'CryG_T
Cuts at: 0 429 2086 3143 3737 4224
Size: 429 1657 1057 594 487
Fragments arranged by size:
1657 1057 594 487 429
AgeI A'CCGG_T
Cuts at: 0 569 4224
Size: 569 3655
AhdI GACnn_n'nnGTC
Cuts at: 0 462 4224
Size: 462 3762
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 52 4224
Size: 52 4172
AlwNI CAG_nnn'CTG
Cuts at: 0 842 1057 4224
Size: 842 215 3167
Fragments arranged by size:
3167 842 215
ApaI G_GGCC'C
Cuts at: 0 981 3272 4224
Size: 981 2291 952
Fragments arranged by size:
2291 981 952
ApaLI G'TGCA_C
Cuts at: 0 1715 4224
Size: 1715 2509
ApoI r'AATT_y
Cuts at: 0 603 1212 1431 1783 2055 2237 2355 2841
Size: 603 609 219 352 272 182 118 486
Cuts at: 2841 3365 3552 3697 4224
Size: 524 187 145 527
Fragments arranged by size:
609 603 527 524 486 352 272 219
187 182 145 118
AvaI C'yCGr_G
Cuts at: 0 2370 4224
Size: 2370 1854
BamHI G'GATC_C
Cuts at: 0 1965 2528 4224
Size: 1965 563 1696
Fragments arranged by size:
1965 1696 563
BanI G'GyrC_C
Cuts at: 0 193 1619 4013 4224
Size: 193 1426 2394 211
Fragments arranged by size:
2394 1426 211 193
BanII G_rGCy'C
Cuts at: 0 506 981 3272 3897 4224
Size: 506 475 2291 625 327
Fragments arranged by size:
2291 625 506 475 327
BbsI GAAGACnn'nnnn_
Cuts at: 0 233 3601 3960 4224
Size: 233 3368 359 264
Fragments arranged by size:
3368 359 264 233
BciVI GTATCCnnnnn_n'
Cuts at: 0 3140 4157 4224
Size: 3140 1017 67
Fragments arranged by size:
3140 1017 67
BclI T'GATC_A
Cuts at: 0 381 4224
Size: 381 3843
BglII A'GATC_T
Cuts at: 0 1676 2940 3543 3591 4224
Size: 1676 1264 603 48 633
Fragments arranged by size:
1676 1264 633 603 48
Bme1580I G_kGCm'C
Cuts at: 0 981 1719 3012 3272 4224
Size: 981 738 1293 260 952
Fragments arranged by size:
1293 981 952 738 260
BmrI ACTGGGnnnn_n'
Cuts at: 0 266 1526 3266 4224
Size: 266 1260 1740 958
Fragments arranged by size:
1740 1260 958 266
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 907 1141 1445 4224
Size: 907 234 304 2779
Fragments arranged by size:
2779 907 304 234
Bpu10I CC'TnA_GC
Cuts at: 0 1654 4224
Size: 1654 2570
BsaI GGTCTCn'nnnn_
Cuts at: 0 1337 3512 4224
Size: 1337 2175 712
Fragments arranged by size:
2175 1337 712
BsaBI GATnn'nnATC
Cuts at: 0 1244 4224
Size: 1244 2980
BsaWI w'CCGG_w
Cuts at: 0 569 4224
Size: 569 3655
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 533 563 4224
Size: 533 30 3661
Fragments arranged by size:
3661 533 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 2975 3859 3905 4224
Size: 75 2900 884 46 319
Fragments arranged by size:
2900 884 319 75 46
BseYI C'CCAG_C
Cuts at: 0 505 980 1754 2133 4224
Size: 505 475 774 379 2091
Fragments arranged by size:
2091 774 505 475 379
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1812 3145 3271 4224
Size: 76 1736 1333 126 953
Fragments arranged by size:
1736 1333 953 126 76
BsiEI CG_ry'CG
Cuts at: 0 1642 4224
Size: 1642 2582
BsiHKAI G_wGCw'C
Cuts at: 0 41 1187 1719 1852 2329 3225 3869 4097
Size: 41 1146 532 133 477 896 644 228
Cuts at: 4097 4224
Size: 127
Fragments arranged by size:
1146 896 644 532 477 228 133 127
41
BsmI GAATG_Cn'
Cuts at: 0 633 864 1090 1654 4224
Size: 633 231 226 564 2570
Fragments arranged by size:
2570 633 564 231 226
Bsp1286I G_dGCh'C
Cuts at: 0 41 506 981 1187 1719 1852 2329 3012
Size: 41 465 475 206 532 133 477 683
Cuts at: 3012 3225 3272 3869 3897 4097 4224
Size: 213 47 597 28 200 127
Fragments arranged by size:
683 597 532 477 475 465 213 206
200 133 127 47 41 28
BspHI T'CATG_A
Cuts at: 0 1179 3443 4224
Size: 1179 2264 781
Fragments arranged by size:
2264 1179 781
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 1380 2759 4224
Size: 427 953 1379 1465
Fragments arranged by size:
1465 1379 953 427
BsrBI CCG'CTC
Cuts at: 0 1658 4224
Size: 1658 2566
BsrDI GCAATG_nn'
Cuts at: 0 72 628 2619 4224
Size: 72 556 1991 1605
Fragments arranged by size:
1991 1605 556 72
BsrFI r'CCGG_y
Cuts at: 0 569 4224
Size: 569 3655
BsrGI T'GTAC_A
Cuts at: 0 2559 3762 4224
Size: 2559 1203 462
Fragments arranged by size:
2559 1203 462
BssHII G'CGCG_C
Cuts at: 0 1231 4224
Size: 1231 2993
BssSI C'ACGA_G
Cuts at: 0 34 4224
Size: 34 4190
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1583 4224
Size: 1583 2641
BstBI TT'CG_AA
Cuts at: 0 3549 3561 4224
Size: 3549 12 663
Fragments arranged by size:
3549 663 12
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3797 4224
Size: 3797 427
BstYI r'GATC_y
Cuts at: 0 919 1535 1676 1965 2220 2528 2940 3543
Size: 919 616 141 289 255 308 412 603
Cuts at: 3543 3591 4224
Size: 48 633
Fragments arranged by size:
919 633 616 603 412 308 289 255
141 48
BtgI C'CryG_G
Cuts at: 0 140 1929 4224
Size: 140 1789 2295
Fragments arranged by size:
2295 1789 140
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2413 4224
Size: 2413 1811
BtsI GCAGTG_nn'
Cuts at: 0 474 1185 2955 3254 4224
Size: 474 711 1770 299 970
Fragments arranged by size:
1770 970 711 474 299
ClaI AT'CG_AT
Cuts at: 0 1074 4224
Size: 1074 3150
DraI TTT'AAA
Cuts at: 0 218 1793 2096 4224
Size: 218 1575 303 2128
Fragments arranged by size:
2128 1575 303 218
DrdI GACnn_nn'nnGTC
Cuts at: 0 693 3144 4224
Size: 693 2451 1080
Fragments arranged by size:
2451 1080 693
EaeI y'GGCC_r
Cuts at: 0 1926 4224
Size: 1926 2298
EarI CTCTTCn'nnn_
Cuts at: 0 494 3303 4224
Size: 494 2809 921
Fragments arranged by size:
2809 921 494
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 907 934 1141 1445 2190 2675 2741
Size: 217 690 27 207 304 745 485 66
Cuts at: 2741 4224
Size: 1483
Fragments arranged by size:
1483 745 690 485 304 217 207 66
27
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 4224
Size: 235 3989
EcoO109I rG'GnC_Cy
Cuts at: 0 6 416 977 4224
Size: 6 410 561 3247
Fragments arranged by size:
3247 561 410 6
EcoRI G'AATT_C
Cuts at: 0 2055 2355 4224
Size: 2055 300 1869
Fragments arranged by size:
2055 1869 300
EcoRV GAT'ATC
Cuts at: 0 641 4064 4224
Size: 641 3423 160
Fragments arranged by size:
3423 641 160
HaeII r_GCGC'y
Cuts at: 0 536 4224
Size: 536 3688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 448 480 533 565 1653 1685 3561 3593
Size: 448 32 53 32 1088 32 1876 32
Cuts at: 3593 3838 3870 4224
Size: 245 32 354
Fragments arranged by size:
1876 1088 448 354 245 53 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 366 408 1110 1134 1281 2425 4224
Size: 366 42 702 24 147 1144 1799
Fragments arranged by size:
1799 1144 702 366 147 42 24
MfeI C'AATT_G
Cuts at: 0 2072 4224
Size: 2072 2152
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 2269 2664 3326 3604 4224
Size: 446 1823 395 662 278 620
Fragments arranged by size:
1823 662 620 446 395 278
MscI TGG'CCA
Cuts at: 0 1928 4224
Size: 1928 2296
MslI CAynn'nnrTG
Cuts at: 0 34 349 877 1866 2386 3293 3464 4224
Size: 34 315 528 989 520 907 171 760
Fragments arranged by size:
989 907 760 528 520 315 171 34
MspA1I CmG'CkG
Cuts at: 0 13 142 2478 3513 4224
Size: 13 129 2336 1035 711
Fragments arranged by size:
2336 1035 711 129 13
NdeI CA'TA_TG
Cuts at: 0 1747 4224
Size: 1747 2477
NspI r_CATG'y
Cuts at: 0 433 2090 2423 3147 3259 3741 4224
Size: 433 1657 333 724 112 482 483
Fragments arranged by size:
1657 724 483 482 433 333 112
PasI CC'CwG_GG
Cuts at: 0 537 4224
Size: 537 3687
PciI A'CATG_T
Cuts at: 0 429 2086 3143 3737 4224
Size: 429 1657 1057 594 487
Fragments arranged by size:
1657 1057 594 487 429
PflMI CCAn_nnn'nTGG
Cuts at: 0 1546 4224
Size: 1546 2678
PfoI T'CCnGG_A
Cuts at: 0 1439 4057 4224
Size: 1439 2618 167
Fragments arranged by size:
2618 1439 167
PpuMI rG'GwC_Cy
Cuts at: 0 416 4224
Size: 416 3808
PspOMI G'GGCC_C
Cuts at: 0 977 3268 4224
Size: 977 2291 956
Fragments arranged by size:
2291 977 956
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1242 1274 4224
Size: 1242 32 2950
Fragments arranged by size:
2950 1242 32
PstI C_TGCA'G
Cuts at: 0 304 1561 1579 4224
Size: 304 1257 18 2645
Fragments arranged by size:
2645 1257 304 18
PvuI CG_AT'CG
Cuts at: 0 1642 4224
Size: 1642 2582
PvuII CAG'CTG
Cuts at: 0 2478 4224
Size: 2478 1746
SacII CC_GC'GG
Cuts at: 0 143 4224
Size: 143 4081
SalI G'TCGA_C
Cuts at: 0 364 4224
Size: 364 3860
SapI GCTCTTCn'nnn_
Cuts at: 0 494 4224
Size: 494 3730
ScaI AGT'ACT
Cuts at: 0 2265 4224
Size: 2265 1959
SfcI C'TryA_G
Cuts at: 0 300 1557 1575 3029 3053 3756 4224
Size: 300 1257 18 1454 24 703 468
Fragments arranged by size:
1454 1257 703 468 300 24 18
SpeI A'CTAG_T
Cuts at: 0 3160 4224
Size: 3160 1064
StyI C'CwwG_G
Cuts at: 0 50 189 1812 2811 4224
Size: 50 139 1623 999 1413
Fragments arranged by size:
1623 1413 999 139 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 165 290 3445 4224
Size: 165 125 3155 779
Fragments arranged by size:
3155 779 165 125
TatI w'GTAC_w
Cuts at: 0 248 1948 2089 2263 2559 2622 2976 3762
Size: 248 1700 141 174 296 63 354 786
Cuts at: 3762 4224
Size: 462
Fragments arranged by size:
1700 786 462 354 296 248 174 141
63
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 14 46 1863 1895 4224
Size: 14 32 1817 32 2329
Fragments arranged by size:
2329 1817 32 32 14
XbaI T'CTAG_A
Cuts at: 0 804 2059 4224
Size: 804 1255 2165
Fragments arranged by size:
2165 1255 804
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 4208 4224
Size: 4208 16
XmnI GAAnn'nnTTC
Cuts at: 0 230 2957 4224
Size: 230 2727 1267
Fragments arranged by size:
2727 1267 230
Enzymes that do cut:
AccI AcuI AflIII AgeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BamHI BanI BanII BbsI BciVI
BclI BglII Bme1580I BmrI BpmI Bpu10I BsaI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
ClaI DraI DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV HaeII Hin4I HincII MfeI MmeI MscI
MslI MspA1I NdeI NspI PasI PciI PflMI PfoI
PpuMI PspOMI PsrI PstI PvuI PvuII SacII SalI
SapI ScaI SfcI SpeI StyI TaqII TatI TstI
XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AleI AloI
AscI AseI AsiSI AvrII BaeI BbeI BbvCI BcgI
BfrBI BglI BlpI BmgBI BmtI BplI BpuEI BsaAI
BsaHI BsiWI BsmBI BspEI BstEII BstZ17I Bsu36I CspCI
DraIII EagI EciI EcoICRI FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MluI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PpiI PshAI PsiI PspXI RsrII SacI SanDI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SphI SrfI SspI StuI SwaI Tth111I XhoI XmaI
ZraI