Restriction map for
pFN21AB5347
(Linear) (Six-base) MAPSORT of: KIBB5347.seq Check: 2592 from: 1 to: 1305
KIBB5347.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 255 1305
Size: 255 1050
AatII G_ACGT'C
Cuts at: 0 524 1305
Size: 524 781
AccI GT'mk_AC
Cuts at: 0 17 1305
Size: 17 1288
Acc65I G'GTAC_C
Cuts at: 0 1241 1305
Size: 1241 64
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 1240 1305
Size: 52 1188 65
Fragments arranged by size:
1188 65 52
AflIII A'CryG_T
Cuts at: 0 77 221 1305
Size: 77 144 1084
Fragments arranged by size:
1084 144 77
AleI CACnn'nnGTG
Cuts at: 0 755 1305
Size: 755 550
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 406 619 1225 1305
Size: 406 213 606 80
Fragments arranged by size:
606 406 213 80
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 730 762 1305
Size: 730 32 543
Fragments arranged by size:
730 543 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1251 1305
Size: 1251 54
ApaI G_GGCC'C
Cuts at: 0 438 1110 1305
Size: 438 672 195
Fragments arranged by size:
672 438 195
AvaI C'yCGr_G
Cuts at: 0 648 658 1042 1080 1305
Size: 648 10 384 38 225
Fragments arranged by size:
648 384 225 38 10
BanI G'GyrC_C
Cuts at: 0 1241 1305
Size: 1241 64
BanII G_rGCy'C
Cuts at: 0 350 438 625 827 1110 1305
Size: 350 88 187 202 283 195
Fragments arranged by size:
350 283 202 195 187 88
BbsI GAAGACnn'nnnn_
Cuts at: 0 633 1145 1305
Size: 633 512 160
Fragments arranged by size:
633 512 160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 597 631 1305
Size: 597 34 674
Fragments arranged by size:
674 597 34
BclI T'GATC_A
Cuts at: 0 917 1305
Size: 917 388
Bme1580I G_kGCm'C
Cuts at: 0 438 986 1110 1305
Size: 438 548 124 195
Fragments arranged by size:
548 438 195 124
BmgBI CAC'GTC
Cuts at: 0 72 852 1305
Size: 72 780 453
Fragments arranged by size:
780 453 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 661 909 1305
Size: 661 248 396
Fragments arranged by size:
661 396 248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 754 1305
Size: 313 441 551
Fragments arranged by size:
551 441 313
BsaAI yAC'GTr
Cuts at: 0 21 1305
Size: 21 1284
BsaBI GATnn'nnATC
Cuts at: 0 868 1305
Size: 868 437
BsaHI Gr'CG_yC
Cuts at: 0 521 1305
Size: 521 784
BsaWI w'CCGG_w
Cuts at: 0 1295 1305
Size: 1295 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 139 310 313 1305
Size: 136 3 171 3 992
Fragments arranged by size:
992 171 136 3 3
BseYI C'CCAG_C
Cuts at: 0 570 1305
Size: 570 735
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 1305
Size: 283 1022
BsiHKAI G_wGCw'C
Cuts at: 0 47 95 350 827 1305
Size: 47 48 255 477 478
Fragments arranged by size:
478 477 255 48 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 844 1305
Size: 844 461
Bsp1286I G_dGCh'C
Cuts at: 0 47 95 350 438 625 827 986 1110
Size: 47 48 255 88 187 202 159 124
Cuts at: 1110 1305
Size: 195
Fragments arranged by size:
255 202 195 187 159 124 88 48
47
BspEI T'CCGG_A
Cuts at: 0 1295 1305
Size: 1295 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 255 1305
Size: 255 1050
BsrDI GCAATG_nn'
Cuts at: 0 603 1305
Size: 603 702
BstAPI GCAn_nnn'nTGC
Cuts at: 0 99 1305
Size: 99 1206
BstXI CCAn_nnnn'nTGG
Cuts at: 0 111 963 1305
Size: 111 852 342
Fragments arranged by size:
852 342 111
BstYI r'GATC_y
Cuts at: 0 386 968 1178 1305
Size: 386 582 210 127
Fragments arranged by size:
582 386 210 127
BtgI C'CryG_G
Cuts at: 0 63 153 439 708 725 1305
Size: 63 90 286 269 17 580
Fragments arranged by size:
580 286 269 90 63 17
EaeI y'GGCC_r
Cuts at: 0 442 1305
Size: 442 863
EarI CTCTTCn'nnn_
Cuts at: 0 1228 1305
Size: 1228 77
EcoICRI GAG'CTC
Cuts at: 0 348 825 1305
Size: 348 477 480
Fragments arranged by size:
480 477 348
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 313 754 1240 1305
Size: 52 261 441 486 65
Fragments arranged by size:
486 441 261 65 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 253 1305
Size: 253 1052
EcoO109I rG'GnC_Cy
Cuts at: 0 434 435 687 1038 1248 1305
Size: 434 1 252 351 210 57
Fragments arranged by size:
434 351 252 210 57 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 708 1305
Size: 708 597
FspI TGC'GCA
Cuts at: 0 607 1305
Size: 607 698
HaeII r_GCGC'y
Cuts at: 0 14 590 1305
Size: 14 576 715
Fragments arranged by size:
715 576 14
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 814 846 982 1014 1305
Size: 814 32 136 32 291
Fragments arranged by size:
814 291 136 32 32
HincII GTy'rAC
Cuts at: 0 537 950 1305
Size: 537 413 355
Fragments arranged by size:
537 413 355
KpnI G_GTAC'C
Cuts at: 0 1245 1305
Size: 1245 60
MluI A'CGCG_T
Cuts at: 0 221 1305
Size: 221 1084
MslI CAynn'nnrTG
Cuts at: 0 109 631 755 979 1207 1305
Size: 109 522 124 224 228 98
Fragments arranged by size:
522 228 224 124 109 98
MspA1I CmG'CkG
Cuts at: 0 441 710 1305
Size: 441 269 595
Fragments arranged by size:
595 441 269
NspI r_CATG'y
Cuts at: 0 81 657 1305
Size: 81 576 648
Fragments arranged by size:
648 576 81
PasI CC'CwG_GG
Cuts at: 0 1256 1305
Size: 1256 49
PciI A'CATG_T
Cuts at: 0 77 1305
Size: 77 1228
PflMI CCAn_nnn'nTGG
Cuts at: 0 423 1305
Size: 423 882
PfoI T'CCnGG_A
Cuts at: 0 308 1305
Size: 308 997
PpuMI rG'GwC_Cy
Cuts at: 0 687 1038 1305
Size: 687 351 267
Fragments arranged by size:
687 351 267
PspOMI G'GGCC_C
Cuts at: 0 434 1106 1305
Size: 434 672 199
Fragments arranged by size:
672 434 199
PstI C_TGCA'G
Cuts at: 0 395 1305
Size: 395 910
RsrII CG'GwC_CG
Cuts at: 0 479 1305
Size: 479 826
SacI G_AGCT'C
Cuts at: 0 350 827 1305
Size: 350 477 478
Fragments arranged by size:
478 477 350
SacII CC_GC'GG
Cuts at: 0 442 711 1305
Size: 442 269 594
Fragments arranged by size:
594 442 269
SanDI GG'GwC_CC
Cuts at: 0 1038 1305
Size: 1038 267
SbfI CC_TGCA'GG
Cuts at: 0 395 1305
Size: 395 910
ScaI AGT'ACT
Cuts at: 0 280 1305
Size: 280 1025
SfcI C'TryA_G
Cuts at: 0 391 1305
Size: 391 914
SmaI CCC'GGG
Cuts at: 0 1082 1305
Size: 1082 223
SphI G_CATG'C
Cuts at: 0 657 1305
Size: 657 648
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 862 1017 1248 1305
Size: 862 155 231 57
Fragments arranged by size:
862 231 155 57
TatI w'GTAC_w
Cuts at: 0 278 398 911 1305
Size: 278 120 513 394
Fragments arranged by size:
513 394 278 120
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 48 80 1305
Size: 48 32 1225
Fragments arranged by size:
1225 48 32
Tth111I GACn'n_nGTC
Cuts at: 0 685 1305
Size: 685 620
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 563 1305
Size: 563 742
XmaI C'CCGG_G
Cuts at: 0 1080 1305
Size: 1080 225
ZraI GAC'GTC
Cuts at: 0 522 1305
Size: 522 783
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AleI AlfI
AloI AlwNI ApaI AvaI BanI BanII BbsI BcgI
BclI Bme1580I BmgBI BplI BpmI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspMI BsrDI BstAPI BstXI BstYI BtgI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KpnI MluI MslI MspA1I NspI PasI PciI
PflMI PfoI PpuMI PspOMI PstI RsrII SacI SacII
SanDI SbfI ScaI SfcI SmaI SphI TaqII TatI
TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AhdI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI
BfrBI BglI BglII BlpI BmrI BmtI Bpu10I BpuEI
BsaI BsaXI BsiEI BsiWI BsmI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI EcoRV FseI FspAI HindIII HpaI KasI MfeI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII SalI SapI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI
SspI StuI StyI SwaI XbaI XhoI XmnI