Restriction map for
pFN21AB5305
(Linear) (Six-base) MAPSORT of: KIBB5305.seq Check: 9531 from: 1 to: 1563
KIBB5305.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1027 1294 1563
Size: 1027 267 269
Fragments arranged by size:
1027 269 267
AccI GT'mk_AC
Cuts at: 0 1322 1563
Size: 1322 241
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 980 1471 1563
Size: 980 491 92
Fragments arranged by size:
980 491 92
ApoI r'AATT_y
Cuts at: 0 1236 1563
Size: 1236 327
AseI AT'TA_AT
Cuts at: 0 191 1563
Size: 191 1372
BanI G'GyrC_C
Cuts at: 0 1012 1399 1563
Size: 1012 387 164
Fragments arranged by size:
1012 387 164
BanII G_rGCy'C
Cuts at: 0 285 1563
Size: 285 1278
BbsI GAAGACnn'nnnn_
Cuts at: 0 1459 1563
Size: 1459 104
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 40 74 1563
Size: 40 34 1489
Fragments arranged by size:
1489 40 34
BfrBI ATG'CAT
Cuts at: 0 1156 1563
Size: 1156 407
BglI GCCn_nnn'nGGC
Cuts at: 0 1472 1563
Size: 1472 91
BglII A'GATC_T
Cuts at: 0 1421 1563
Size: 1421 142
Bme1580I G_kGCm'C
Cuts at: 0 1015 1563
Size: 1015 548
BmrI ACTGGGnnnn_n'
Cuts at: 0 1151 1563
Size: 1151 412
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1563
Size: 224 1339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1563
Size: 1043 520
BsaWI w'CCGG_w
Cuts at: 0 63 650 1563
Size: 63 587 913
Fragments arranged by size:
913 587 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 818 848 1563
Size: 818 30 715
Fragments arranged by size:
818 715 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1078 1563
Size: 1078 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1563
Size: 622 941
BsiEI CG_ry'CG
Cuts at: 0 68 1563
Size: 68 1495
Bsp1286I G_dGCh'C
Cuts at: 0 285 1015 1563
Size: 285 730 548
Fragments arranged by size:
730 548 285
BspHI T'CATG_A
Cuts at: 0 498 1563
Size: 498 1065
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1027 1294 1563
Size: 1027 267 269
Fragments arranged by size:
1027 269 267
BsrDI GCAATG_nn'
Cuts at: 0 569 1106 1259 1563
Size: 569 537 153 304
Fragments arranged by size:
569 537 304 153
BsrFI r'CCGG_y
Cuts at: 0 1267 1563
Size: 1267 296
BstAPI GCAn_nnn'nTGC
Cuts at: 0 636 1019 1563
Size: 636 383 544
Fragments arranged by size:
636 544 383
BstXI CCAn_nnnn'nTGG
Cuts at: 0 732 1123 1563
Size: 732 391 440
Fragments arranged by size:
732 440 391
BstYI r'GATC_y
Cuts at: 0 1067 1421 1563
Size: 1067 354 142
Fragments arranged by size:
1067 354 142
BtgI C'CryG_G
Cuts at: 0 23 1377 1563
Size: 23 1354 186
Fragments arranged by size:
1354 186 23
BtsI GCAGTG_nn'
Cuts at: 0 1278 1563
Size: 1278 285
DrdI GACnn_nn'nnGTC
Cuts at: 0 316 1563
Size: 316 1247
EaeI y'GGCC_r
Cuts at: 0 125 734 1464 1563
Size: 125 609 730 99
Fragments arranged by size:
730 609 125 99
EarI CTCTTCn'nnn_
Cuts at: 0 24 98 218 378 470 572 806 1563
Size: 24 74 120 160 92 102 234 757
Fragments arranged by size:
757 234 160 120 102 92 74 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 980 1471 1563
Size: 224 756 491 92
Fragments arranged by size:
756 491 224 92
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1075 1563
Size: 1075 488
EcoO109I rG'GnC_Cy
Cuts at: 0 370 617 1173 1299 1538 1563
Size: 370 247 556 126 239 25
Fragments arranged by size:
556 370 247 239 126 25
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 178 346 1563
Size: 178 168 1217
Fragments arranged by size:
1217 178 168
HaeII r_GCGC'y
Cuts at: 0 1041 1563
Size: 1041 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 105 137 442 474 1059 1091 1178 1210
Size: 105 32 305 32 585 32 87 32
Cuts at: 1210 1563
Size: 353
Fragments arranged by size:
585 353 305 105 87 32 32 32
32
HincII GTy'rAC
Cuts at: 0 879 1563
Size: 879 684
HindIII A'AGCT_T
Cuts at: 0 323 913 1563
Size: 323 590 650
Fragments arranged by size:
650 590 323
MfeI C'AATT_G
Cuts at: 0 830 1563
Size: 830 733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 727 1329 1563
Size: 727 602 234
Fragments arranged by size:
727 602 234
MscI TGG'CCA
Cuts at: 0 127 736 1466 1563
Size: 127 609 730 97
Fragments arranged by size:
730 609 127 97
MslI CAynn'nnrTG
Cuts at: 0 1096 1121 1563
Size: 1096 25 442
Fragments arranged by size:
1096 442 25
MspA1I CmG'CkG
Cuts at: 0 1311 1449 1563
Size: 1311 138 114
Fragments arranged by size:
1311 138 114
NcoI C'CATG_G
Cuts at: 0 1377 1563
Size: 1377 186
NdeI CA'TA_TG
Cuts at: 0 1530 1563
Size: 1530 33
NruI TCG'CGA
Cuts at: 0 12 1563
Size: 12 1551
NsiI A_TGCA'T
Cuts at: 0 1158 1563
Size: 1158 405
PasI CC'CwG_GG
Cuts at: 0 557 1563
Size: 557 1006
PflMI CCAn_nnn'nTGG
Cuts at: 0 1092 1424 1563
Size: 1092 332 139
Fragments arranged by size:
1092 332 139
PpuMI rG'GwC_Cy
Cuts at: 0 370 617 1173 1299 1538 1563
Size: 370 247 556 126 239 25
Fragments arranged by size:
556 370 247 239 126 25
PshAI GACnn'nnGTC
Cuts at: 0 1357 1563
Size: 1357 206
SapI GCTCTTCn'nnn_
Cuts at: 0 218 1563
Size: 218 1345
SexAI A'CCwGG_T
Cuts at: 0 490 1563
Size: 490 1073
SmlI C'TyrA_G
Cuts at: 0 1058 1563
Size: 1058 505
SspI AAT'ATT
Cuts at: 0 189 1563
Size: 189 1374
StyI C'CwwG_G
Cuts at: 0 338 860 1377 1563
Size: 338 522 517 186
Fragments arranged by size:
522 517 338 186
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1084 1448 1563
Size: 1084 364 115
Fragments arranged by size:
1084 364 115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 858 1563
Size: 858 705
Enzymes that do cut:
AarI AccI AcuI ApoI AseI BanI BanII BbsI
BcgI BfrBI BglI BglII Bme1580I BmrI BpmI BpuEI
BsaWI BsaXI BseRI BsgI BsiEI Bsp1286I BspHI BspMI
BsrDI BsrFI BstAPI BstXI BstYI BtgI BtsI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
HincII HindIII MfeI MmeI MscI MslI MspA1I NcoI
NdeI NruI NsiI PasI PflMI PpuMI PshAI SapI
SexAI SmlI SspI StyI TaqII XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BciVI BclI
BlpI BmgBI BmtI BplI Bpu10I BsaI BsaAI BsaBI
BsaHI BseYI BsiHKAI BsiWI BsmI BsmBI BspEI BsrBI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HpaI KasI KpnI MluI
NaeI NarI NgoMIV NheI NotI NspI PacI PciI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI