Restriction map for
pFN21AB5246
(Linear) (Six-base) MAPSORT of: KIBB5246.seq Check: 266 from: 1 to: 1440
KIBB5246.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AatII G_ACGT'C
Cuts at: 0 1298 1352 1440
Size: 1298 54 88
Fragments arranged by size:
1298 88 54
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1213 1440
Size: 715 498 227
Fragments arranged by size:
715 498 227
AflIII A'CryG_T
Cuts at: 0 179 1440
Size: 179 1261
AlwNI CAG_nnn'CTG
Cuts at: 0 1273 1440
Size: 1273 167
ApaI G_GGCC'C
Cuts at: 0 174 244 1440
Size: 174 70 1196
Fragments arranged by size:
1196 174 70
AscI GG'CGCG_CC
Cuts at: 0 206 1440
Size: 206 1234
AvaI C'yCGr_G
Cuts at: 0 74 173 211 981 1440
Size: 74 99 38 770 459
Fragments arranged by size:
770 459 99 74 38
BanI G'GyrC_C
Cuts at: 0 51 90 107 217 270 282 594 703
Size: 51 39 17 110 53 12 312 109
Cuts at: 703 1202 1440
Size: 499 238
Fragments arranged by size:
499 312 238 110 109 53 51 39
17 12
BanII G_rGCy'C
Cuts at: 0 174 244 926 1440
Size: 174 70 682 514
Fragments arranged by size:
682 514 174 70
BbeI G_GCGC'C
Cuts at: 0 55 94 221 1440
Size: 55 39 127 1219
Fragments arranged by size:
1219 127 55 39
BciVI GTATCCnnnnn_n'
Cuts at: 0 1162 1440
Size: 1162 278
BclI T'GATC_A
Cuts at: 0 1409 1440
Size: 1409 31
BglI GCCn_nnn'nGGC
Cuts at: 0 248 1440
Size: 248 1192
Bme1580I G_kGCm'C
Cuts at: 0 112 174 244 275 1440
Size: 112 62 70 31 1165
Fragments arranged by size:
1165 112 70 62 31
BmgBI CAC'GTC
Cuts at: 0 522 1172 1221 1440
Size: 522 650 49 219
Fragments arranged by size:
650 522 219 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 1440
Size: 1267 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 1285 1332 1440
Size: 1285 47 108
Fragments arranged by size:
1285 108 47
BsaAI yAC'GTr
Cuts at: 0 660 1440
Size: 660 780
BsaHI Gr'CG_yC
Cuts at: 0 52 91 218 365 959 1295 1349 1440
Size: 52 39 127 147 594 336 54 91
Fragments arranged by size:
594 336 147 127 91 54 52 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 229 259 818 848 1440
Size: 229 30 559 30 592
Fragments arranged by size:
592 559 229 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 798 1440
Size: 798 642
BseYI C'CCAG_C
Cuts at: 0 899 918 1263 1440
Size: 899 19 345 177
Fragments arranged by size:
899 345 177 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 1440
Size: 409 1031
BsiEI CG_ry'CG
Cuts at: 0 84 168 433 823 1440
Size: 84 84 265 390 617
Fragments arranged by size:
617 390 265 84 84
BsiHKAI G_wGCw'C
Cuts at: 0 155 895 1440
Size: 155 740 545
Fragments arranged by size:
740 545 155
Bsp1286I G_dGCh'C
Cuts at: 0 112 155 174 244 275 895 926 1440
Size: 112 43 19 70 31 620 31 514
Fragments arranged by size:
620 514 112 70 43 31 31 19
BspHI T'CATG_A
Cuts at: 0 1406 1440
Size: 1406 34
BsrBI CCG'CTC
Cuts at: 0 19 86 413 806 1440
Size: 19 67 327 393 634
Fragments arranged by size:
634 393 327 67 19
BsrFI r'CCGG_y
Cuts at: 0 79 126 319 348 428 435 597 1199
Size: 79 47 193 29 80 7 162 602
Cuts at: 1199 1440
Size: 241
Fragments arranged by size:
602 241 193 162 80 79 47 29
7
BssHII G'CGCG_C
Cuts at: 0 12 130 206 309 311 396 1440
Size: 12 118 76 103 2 85 1044
Fragments arranged by size:
1044 118 103 85 76 12 2
BstEII G'GTnAC_C
Cuts at: 0 663 1440
Size: 663 777
BstYI r'GATC_y
Cuts at: 0 5 480 1440
Size: 5 475 960
Fragments arranged by size:
960 475 5
BtgI C'CryG_G
Cuts at: 0 296 545 1322 1440
Size: 296 249 777 118
Fragments arranged by size:
777 296 249 118
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 735 1440
Size: 735 705
BtsI GCAGTG_nn'
Cuts at: 0 153 268 1440
Size: 153 115 1172
Fragments arranged by size:
1172 153 115
DraIII CAC_nnn'GTG
Cuts at: 0 663 1440
Size: 663 777
EaeI y'GGCC_r
Cuts at: 0 81 98 165 293 430 465 801 820
Size: 81 17 67 128 137 35 336 19
Cuts at: 820 1440
Size: 620
Fragments arranged by size:
620 336 137 128 81 67 35 19
17
EagI C'GGCC_G
Cuts at: 0 81 165 430 820 1440
Size: 81 84 265 390 620
Fragments arranged by size:
620 390 265 84 81
EarI CTCTTCn'nnn_
Cuts at: 0 26 1440
Size: 26 1414
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1320 1440
Size: 1320 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 715 1213 1440
Size: 715 498 227
Fragments arranged by size:
715 498 227
EcoNI CCTnn'n_nnAGG
Cuts at: 0 235 1440
Size: 235 1205
EcoO109I rG'GnC_Cy
Cuts at: 0 240 1440
Size: 240 1200
EcoRV GAT'ATC
Cuts at: 0 1404 1440
Size: 1404 36
FseI GG_CCGG'CC
Cuts at: 0 83 323 1440
Size: 83 240 1117
Fragments arranged by size:
1117 240 83
HaeII r_GCGC'y
Cuts at: 0 55 94 221 1440
Size: 55 39 127 1219
Fragments arranged by size:
1219 127 55 39
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1440
Size: 1210 32 198
Fragments arranged by size:
1210 198 32
HindIII A'AGCT_T
Cuts at: 0 571 1440
Size: 571 869
KasI G'GCGC_C
Cuts at: 0 51 90 217 1440
Size: 51 39 127 1223
Fragments arranged by size:
1223 127 51 39
MluI A'CGCG_T
Cuts at: 0 179 1440
Size: 179 1261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 608 1440
Size: 608 832
MspA1I CmG'CkG
Cuts at: 0 30 69 119 393 1045 1440
Size: 30 39 50 274 652 395
Fragments arranged by size:
652 395 274 50 39 30
NaeI GCC'GGC
Cuts at: 0 81 128 321 1440
Size: 81 47 193 1119
Fragments arranged by size:
1119 193 81 47
NarI GG'CG_CC
Cuts at: 0 52 91 218 1440
Size: 52 39 127 1222
Fragments arranged by size:
1222 127 52 39
NgoMIV G'CCGG_C
Cuts at: 0 79 126 319 1440
Size: 79 47 193 1121
Fragments arranged by size:
1121 193 79 47
PmlI CAC'GTG
Cuts at: 0 660 1440
Size: 660 780
PspOMI G'GGCC_C
Cuts at: 0 170 240 1440
Size: 170 70 1200
Fragments arranged by size:
1200 170 70
PstI C_TGCA'G
Cuts at: 0 236 263 452 1440
Size: 236 27 189 988
Fragments arranged by size:
988 236 189 27
PvuII CAG'CTG
Cuts at: 0 69 393 1045 1440
Size: 69 324 652 395
Fragments arranged by size:
652 395 324 69
SapI GCTCTTCn'nnn_
Cuts at: 0 26 1440
Size: 26 1414
SbfI CC_TGCA'GG
Cuts at: 0 236 1440
Size: 236 1204
SfcI C'TryA_G
Cuts at: 0 232 259 448 1440
Size: 232 27 189 992
Fragments arranged by size:
992 232 189 27
SfoI GGC'GCC
Cuts at: 0 53 92 219 1440
Size: 53 39 127 1221
Fragments arranged by size:
1221 127 53 39
SmaI CCC'GGG
Cuts at: 0 76 175 1440
Size: 76 99 1265
Fragments arranged by size:
1265 99 76
SmlI C'TyrA_G
Cuts at: 0 1282 1440
Size: 1282 158
StyI C'CwwG_G
Cuts at: 0 468 1440
Size: 468 972
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 913 970 1121 1440
Size: 913 57 151 319
Fragments arranged by size:
913 319 151 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 229 261 629 661 818 850 1307 1339
Size: 229 32 368 32 157 32 457 32
Cuts at: 1339 1404 1436 1440
Size: 65 32 4
Fragments arranged by size:
457 368 229 157 65 32 32 32
32 32 4
XmaI C'CCGG_G
Cuts at: 0 74 173 1440
Size: 74 99 1267
Fragments arranged by size:
1267 99 74
XmnI GAAnn'nnTTC
Cuts at: 0 563 1250 1440
Size: 563 687 190
Fragments arranged by size:
687 563 190
ZraI GAC'GTC
Cuts at: 0 1296 1350 1440
Size: 1296 54 90
Fragments arranged by size:
1296 90 54
Enzymes that do cut:
AatII AcuI AflIII AlwNI ApaI AscI AvaI BanI
BanII BbeI BciVI BclI BglI Bme1580I BmgBI BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspHI BsrBI BsrFI BssHII BstEII BstYI
BtgI BtgZI BtsI DraIII EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRV FseI HaeII Hin4I HindIII
KasI MluI MmeI MspA1I NaeI NarI NgoMIV PmlI
PspOMI PstI PvuII SapI SbfI SfcI SfoI SmaI
SmlI StyI TaqII TstI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BfrBI BglII BlpI
BmrI BmtI BplI BpmI Bpu10I BsaBI BsaWI BsiWI
BsmI BsmBI BspEI BspMI BsrDI BsrGI BssSI BstAPI
BstBI BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI
EcoICRI EcoRI FalI FspI FspAI HincII HpaI KpnI
MfeI MscI MslI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XcmI XhoI