Restriction map for
pFN21AB5174
(Linear) (Six-base) MAPSORT of: KIBB5174.seq Check: 8396 from: 1 to: 1194
KIBB5174.orf
With 182 enzymes: SgfI *
July 18, 2007 13:37 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1062 1194
Size: 1062 132
AfeI AGC'GCT
Cuts at: 0 509 1093 1194
Size: 509 584 101
Fragments arranged by size:
584 509 101
ApaI G_GGCC'C
Cuts at: 0 425 796 1194
Size: 425 371 398
Fragments arranged by size:
425 398 371
ApaLI G'TGCA_C
Cuts at: 0 626 1148 1194
Size: 626 522 46
Fragments arranged by size:
626 522 46
AvaI C'yCGr_G
Cuts at: 0 493 688 713 993 1059 1194
Size: 493 195 25 280 66 135
Fragments arranged by size:
493 280 195 135 66 25
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1157 1190 1194
Size: 1157 33 4
Fragments arranged by size:
1157 33 4
BanI G'GyrC_C
Cuts at: 0 24 97 256 671 1194
Size: 24 73 159 415 523
Fragments arranged by size:
523 415 159 73 24
BanII G_rGCy'C
Cuts at: 0 425 500 796 1194
Size: 425 75 296 398
Fragments arranged by size:
425 398 296 75
BbeI G_GCGC'C
Cuts at: 0 28 260 675 1194
Size: 28 232 415 519
Fragments arranged by size:
519 415 232 28
BbsI GAAGACnn'nnnn_
Cuts at: 0 336 1194
Size: 336 858
BbvCI CC'TCA_GC
Cuts at: 0 1025 1194
Size: 1025 169
BglI GCCn_nnn'nGGC
Cuts at: 0 684 781 1194
Size: 684 97 413
Fragments arranged by size:
684 413 97
BlpI GC'TnA_GC
Cuts at: 0 370 757 1194
Size: 370 387 437
Fragments arranged by size:
437 387 370
Bme1580I G_kGCm'C
Cuts at: 0 425 630 796 832 1152 1194
Size: 425 205 166 36 320 42
Fragments arranged by size:
425 320 205 166 42 36
BmgBI CAC'GTC
Cuts at: 0 822 1194
Size: 822 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 420 1194
Size: 412 8 774
Fragments arranged by size:
774 412 8
Bpu10I CC'TnA_GC
Cuts at: 0 1025 1194
Size: 1025 169
BsaI GGTCTCn'nnnn_
Cuts at: 0 983 1194
Size: 983 211
BsaAI yAC'GTr
Cuts at: 0 579 1194
Size: 579 615
BsaHI Gr'CG_yC
Cuts at: 0 25 116 186 257 341 672 972 1194
Size: 25 91 70 71 84 331 300 222
Fragments arranged by size:
331 300 222 91 84 71 70 25
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 529 559 1194
Size: 529 30 635
Fragments arranged by size:
635 529 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 481 1194
Size: 481 713
BseYI C'CCAG_C
Cuts at: 0 510 1004 1103 1194
Size: 510 494 99 91
Fragments arranged by size:
510 494 99 91
BsiEI CG_ry'CG
Cuts at: 0 22 1194
Size: 22 1172
BsiHKAI G_wGCw'C
Cuts at: 0 182 584 596 630 770 1152 1194
Size: 182 402 12 34 140 382 42
Fragments arranged by size:
402 382 182 140 42 34 12
BsmBI CGTCTCn'nnnn_
Cuts at: 0 438 1194
Size: 438 756
Bsp1286I G_dGCh'C
Cuts at: 0 182 425 500 584 596 630 770 796
Size: 182 243 75 84 12 34 140 26
Cuts at: 796 832 1152 1194
Size: 36 320 42
Fragments arranged by size:
320 243 182 140 84 75 42 36
34 26 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 944 1194
Size: 944 250
BsrBI CCG'CTC
Cuts at: 0 294 561 1194
Size: 294 267 633
Fragments arranged by size:
633 294 267
BsrFI r'CCGG_y
Cuts at: 0 21 27 64 100 678 1182 1194
Size: 21 6 37 36 578 504 12
Fragments arranged by size:
578 504 37 36 21 12 6
BssHII G'CGCG_C
Cuts at: 0 82 108 412 637 835 1194
Size: 82 26 304 225 198 359
Fragments arranged by size:
359 304 225 198 82 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 358 647 1194
Size: 358 289 547
Fragments arranged by size:
547 358 289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 390 1194
Size: 390 804
BstYI r'GATC_y
Cuts at: 0 243 1194
Size: 243 951
BtgI C'CryG_G
Cuts at: 0 75 119 407 467 884 1194
Size: 75 44 288 60 417 310
Fragments arranged by size:
417 310 288 75 60 44
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 369 472 1082 1194
Size: 369 103 610 112
Fragments arranged by size:
610 369 112 103
BtsI GCAGTG_nn'
Cuts at: 0 356 1194
Size: 356 838
EaeI y'GGCC_r
Cuts at: 0 19 380 614 881 887 1194
Size: 19 361 234 267 6 307
Fragments arranged by size:
361 307 267 234 19 6
EagI C'GGCC_G
Cuts at: 0 19 1194
Size: 19 1175
EarI CTCTTCn'nnn_
Cuts at: 0 589 805 1194
Size: 589 216 389
Fragments arranged by size:
589 389 216
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 420 1062 1194
Size: 412 8 642 132
Fragments arranged by size:
642 412 132 8
EcoO109I rG'GnC_Cy
Cuts at: 0 421 792 793 1038 1194
Size: 421 371 1 245 156
Fragments arranged by size:
421 371 245 156 1
FspI TGC'GCA
Cuts at: 0 751 1194
Size: 751 443
HaeII r_GCGC'y
Cuts at: 0 28 260 511 675 1095 1194
Size: 28 232 251 164 420 99
Fragments arranged by size:
420 251 232 164 99 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 529 561 1081 1113 1194
Size: 529 32 520 32 81
Fragments arranged by size:
529 520 81 32 32
KasI G'GCGC_C
Cuts at: 0 24 256 671 1194
Size: 24 232 415 523
Fragments arranged by size:
523 415 232 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 1194
Size: 221 973
MscI TGG'CCA
Cuts at: 0 382 883 889 1194
Size: 382 501 6 305
Fragments arranged by size:
501 382 305 6
MspA1I CmG'CkG
Cuts at: 0 77 469 1049 1082 1127 1194
Size: 77 392 580 33 45 67
Fragments arranged by size:
580 392 77 67 45 33
NaeI GCC'GGC
Cuts at: 0 23 102 680 1194
Size: 23 79 578 514
Fragments arranged by size:
578 514 79 23
NarI GG'CG_CC
Cuts at: 0 25 257 672 1194
Size: 25 232 415 522
Fragments arranged by size:
522 415 232 25
NcoI C'CATG_G
Cuts at: 0 407 884 1194
Size: 407 477 310
Fragments arranged by size:
477 407 310
NgoMIV G'CCGG_C
Cuts at: 0 21 100 678 1194
Size: 21 79 578 516
Fragments arranged by size:
578 516 79 21
NspI r_CATG'y
Cuts at: 0 788 1194
Size: 788 406
PspOMI G'GGCC_C
Cuts at: 0 421 792 1194
Size: 421 371 402
Fragments arranged by size:
421 402 371
PstI C_TGCA'G
Cuts at: 0 860 1191 1194
Size: 860 331 3
Fragments arranged by size:
860 331 3
SacII CC_GC'GG
Cuts at: 0 78 470 1194
Size: 78 392 724
Fragments arranged by size:
724 392 78
SapI GCTCTTCn'nnn_
Cuts at: 0 589 1194
Size: 589 605
SbfI CC_TGCA'GG
Cuts at: 0 860 1194
Size: 860 334
SfcI C'TryA_G
Cuts at: 0 856 1187 1194
Size: 856 331 7
Fragments arranged by size:
856 331 7
SfoI GGC'GCC
Cuts at: 0 26 258 673 1194
Size: 26 232 415 521
Fragments arranged by size:
521 415 232 26
SgrAI Cr'CCGG_yG
Cuts at: 0 27 1194
Size: 27 1167
SmaI CCC'GGG
Cuts at: 0 690 715 1061 1194
Size: 690 25 346 133
Fragments arranged by size:
690 346 133 25
StuI AGG'CCT
Cuts at: 0 574 1194
Size: 574 620
StyI C'CwwG_G
Cuts at: 0 407 569 884 1194
Size: 407 162 315 310
Fragments arranged by size:
407 315 310 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1167 1194
Size: 1167 27
TatI w'GTAC_w
Cuts at: 0 849 1194
Size: 849 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 512 1194
Size: 512 682
XmaI C'CCGG_G
Cuts at: 0 688 713 1059 1194
Size: 688 25 346 135
Fragments arranged by size:
688 346 135 25
Enzymes that do cut:
AcuI AfeI ApaI ApaLI AvaI BaeI BanI BanII
BbeI BbsI BbvCI BglI BlpI Bme1580I BmgBI BpmI
Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
Eco57MI EcoO109I FspI HaeII Hin4I KasI MmeI MscI
MspA1I NaeI NarI NcoI NgoMIV NspI PspOMI PstI
SacII SapI SbfI SfcI SfoI SgrAI SmaI StuI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApoI AscI AseI
AsiSI AvrII BamHI BcgI BciVI BclI BfrBI BglII
BmrI BmtI BplI BpuEI BsaBI BsaWI BsgI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EciI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI HincII
HindIII HpaI KpnI MfeI MluI MslI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI