Restriction map for
pFN21AB5104
(Linear) (Six-base) MAPSORT of: KIBB5104.seq Check: 5689 from: 1 to: 1644
KIBB5104.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 125 178 271 1644
Size: 35 90 53 93 1373
Fragments arranged by size:
1373 93 90 53 35
AflIII A'CryG_T
Cuts at: 0 664 1644
Size: 664 980
AleI CACnn'nnGTG
Cuts at: 0 253 1644
Size: 253 1391
AlwNI CAG_nnn'CTG
Cuts at: 0 294 297 1644
Size: 294 3 1347
Fragments arranged by size:
1347 294 3
ApoI r'AATT_y
Cuts at: 0 185 304 421 655 1011 1557 1644
Size: 185 119 117 234 356 546 87
Fragments arranged by size:
546 356 234 185 119 117 87
AseI AT'TA_AT
Cuts at: 0 1202 1644
Size: 1202 442
BanI G'GyrC_C
Cuts at: 0 99 1356 1644
Size: 99 1257 288
Fragments arranged by size:
1257 288 99
BanII G_rGCy'C
Cuts at: 0 69 995 1644
Size: 69 926 649
Fragments arranged by size:
926 649 69
BbsI GAAGACnn'nnnn_
Cuts at: 0 1088 1644
Size: 1088 556
BbvCI CC'TCA_GC
Cuts at: 0 416 1644
Size: 416 1228
BclI T'GATC_A
Cuts at: 0 407 1644
Size: 407 1237
Bme1580I G_kGCm'C
Cuts at: 0 102 1317 1644
Size: 102 1215 327
Fragments arranged by size:
1215 327 102
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 1570 1644
Size: 1328 242 74
Fragments arranged by size:
1328 242 74
Bpu10I CC'TnA_GC
Cuts at: 0 416 1644
Size: 416 1228
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1251 1644
Size: 1111 140 393
Fragments arranged by size:
1111 393 140
BsaHI Gr'CG_yC
Cuts at: 0 1626 1644
Size: 1626 18
BsaWI w'CCGG_w
Cuts at: 0 1015 1644
Size: 1015 629
BseYI C'CCAG_C
Cuts at: 0 994 1644
Size: 994 650
BsiHKAI G_wGCw'C
Cuts at: 0 69 1644
Size: 69 1575
BsmI GAATG_Cn'
Cuts at: 0 353 453 1644
Size: 353 100 1191
Fragments arranged by size:
1191 353 100
Bsp1286I G_dGCh'C
Cuts at: 0 69 102 995 1317 1644
Size: 69 33 893 322 327
Fragments arranged by size:
893 327 322 69 33
BspEI T'CCGG_A
Cuts at: 0 1015 1644
Size: 1015 629
BstAPI GCAn_nnn'nTGC
Cuts at: 0 297 736 1644
Size: 297 439 908
Fragments arranged by size:
908 439 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 535 942 1002 1644
Size: 535 407 60 642
Fragments arranged by size:
642 535 407 60
BstYI r'GATC_y
Cuts at: 0 360 720 1644
Size: 360 360 924
Fragments arranged by size:
924 360 360
BtgI C'CryG_G
Cuts at: 0 935 1644
Size: 935 709
BtsI GCAGTG_nn'
Cuts at: 0 260 1644
Size: 260 1384
DraI TTT'AAA
Cuts at: 0 440 1644
Size: 440 1204
EarI CTCTTCn'nnn_
Cuts at: 0 329 1571 1644
Size: 329 1242 73
Fragments arranged by size:
1242 329 73
EcoICRI GAG'CTC
Cuts at: 0 67 1644
Size: 67 1577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 125 178 271 1328 1570 1644
Size: 35 90 53 93 1057 242 74
Fragments arranged by size:
1057 242 93 90 74 53 35
EcoO109I rG'GnC_Cy
Cuts at: 0 593 1644
Size: 593 1051
EcoRI G'AATT_C
Cuts at: 0 421 655 1644
Size: 421 234 989
Fragments arranged by size:
989 421 234
EcoRV GAT'ATC
Cuts at: 0 573 1644
Size: 573 1071
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 110 301 741 1644
Size: 110 191 440 903
Fragments arranged by size:
903 440 191 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 1357 1389 1644
Size: 110 32 1215 32 255
Fragments arranged by size:
1215 255 110 32 32
HincII GTy'rAC
Cuts at: 0 1253 1644
Size: 1253 391
HindIII A'AGCT_T
Cuts at: 0 1235 1644
Size: 1235 409
HpaI GTT'AAC
Cuts at: 0 1253 1644
Size: 1253 391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1180 1383 1644
Size: 1180 203 261
Fragments arranged by size:
1180 261 203
MslI CAynn'nnrTG
Cuts at: 0 137 253 929 1391 1644
Size: 137 116 676 462 253
Fragments arranged by size:
676 462 253 137 116
MspA1I CmG'CkG
Cuts at: 0 198 294 949 1644
Size: 198 96 655 695
Fragments arranged by size:
695 655 198 96
NcoI C'CATG_G
Cuts at: 0 935 1644
Size: 935 709
NdeI CA'TA_TG
Cuts at: 0 134 1644
Size: 134 1510
NspI r_CATG'y
Cuts at: 0 211 1644
Size: 211 1433
PpuMI rG'GwC_Cy
Cuts at: 0 593 1644
Size: 593 1051
PsiI TTA'TAA
Cuts at: 0 383 1644
Size: 383 1261
PvuII CAG'CTG
Cuts at: 0 198 294 949 1644
Size: 198 96 655 695
Fragments arranged by size:
695 655 198 96
SacI G_AGCT'C
Cuts at: 0 69 1644
Size: 69 1575
SfcI C'TryA_G
Cuts at: 0 1174 1644
Size: 1174 470
SmlI C'TyrA_G
Cuts at: 0 1126 1266 1644
Size: 1126 140 378
Fragments arranged by size:
1126 378 140
SpeI A'CTAG_T
Cuts at: 0 985 1644
Size: 985 659
StuI AGG'CCT
Cuts at: 0 758 1644
Size: 758 886
StyI C'CwwG_G
Cuts at: 0 935 1644
Size: 935 709
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1260 1644
Size: 1260 384
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 656 688 1644
Size: 656 32 956
Fragments arranged by size:
956 656 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1350 1644
Size: 1350 294
XmnI GAAnn'nnTTC
Cuts at: 0 629 655 1532 1644
Size: 629 26 877 112
Fragments arranged by size:
877 629 112 26
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApoI AseI BanI BanII
BbsI BbvCI BclI Bme1580I BpmI Bpu10I BpuEI BsaHI
BsaWI BseYI BsiHKAI BsmI Bsp1286I BspEI BstAPI BstXI
BstYI BtgI BtsI DraI EarI EcoICRI Eco57MI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII HpaI MmeI
MslI MspA1I NcoI NdeI NspI PpuMI PsiI PvuII
SacI SfcI SmlI SpeI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BsaI BsaAI
BsaBI BsaXI BseRI BsgI BsiEI BsiWI BsmBI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EaeI EagI EciI EcoNI FseI FspI FspAI HaeII
KasI KpnI MfeI MluI MscI NaeI NarI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI