Restriction map for
pFN21AB5056
(Linear) (Six-base) MAPSORT of: KIBB5056.seq Check: 8342 from: 1 to: 1671
KIBB5056.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AatII G_ACGT'C
Cuts at: 0 566 1671
Size: 566 1105
AccI GT'mk_AC
Cuts at: 0 566 1671
Size: 566 1105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 292 504 1047 1671
Size: 216 76 212 543 624
Fragments arranged by size:
624 543 216 212 76
AflIII A'CryG_T
Cuts at: 0 1552 1562 1671
Size: 1552 10 109
Fragments arranged by size:
1552 109 10
AhdI GACnn_n'nnGTC
Cuts at: 0 387 1484 1671
Size: 387 1097 187
Fragments arranged by size:
1097 387 187
AlwNI CAG_nnn'CTG
Cuts at: 0 215 1671
Size: 215 1456
ApaI G_GGCC'C
Cuts at: 0 49 309 555 1124 1671
Size: 49 260 246 569 547
Fragments arranged by size:
569 547 260 246 49
ApaLI G'TGCA_C
Cuts at: 0 1509 1671
Size: 1509 162
ApoI r'AATT_y
Cuts at: 0 1093 1671
Size: 1093 578
AvaI C'yCGr_G
Cuts at: 0 981 1117 1465 1671
Size: 981 136 348 206
Fragments arranged by size:
981 348 206 136
BanII G_rGCy'C
Cuts at: 0 49 122 163 309 555 1124 1671
Size: 49 73 41 146 246 569 547
Fragments arranged by size:
569 547 246 146 73 49 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 474 918 1671
Size: 474 444 753
Fragments arranged by size:
753 474 444
BbvCI CC'TCA_GC
Cuts at: 0 181 1180 1671
Size: 181 999 491
Fragments arranged by size:
999 491 181
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 432 466 1671
Size: 432 34 1205
Fragments arranged by size:
1205 432 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1324 1671
Size: 1324 347
BglI GCCn_nnn'nGGC
Cuts at: 0 748 1190 1671
Size: 748 442 481
Fragments arranged by size:
748 481 442
BlpI GC'TnA_GC
Cuts at: 0 1393 1671
Size: 1393 278
Bme1580I G_kGCm'C
Cuts at: 0 49 309 555 1118 1124 1365 1513 1671
Size: 49 260 246 563 6 241 148 158
Fragments arranged by size:
563 260 246 241 158 148 49 6
BmgBI CAC'GTC
Cuts at: 0 861 1671
Size: 861 810
BmrI ACTGGGnnnn_n'
Cuts at: 0 1352 1671
Size: 1352 319
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 318 1671
Size: 318 1353
Bpu10I CC'TnA_GC
Cuts at: 0 181 1180 1671
Size: 181 999 491
Fragments arranged by size:
999 491 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 1671
Size: 1579 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 949 1671
Size: 949 722
BsaAI yAC'GTr
Cuts at: 0 1025 1400 1671
Size: 1025 375 271
Fragments arranged by size:
1025 375 271
BsaBI GATnn'nnATC
Cuts at: 0 1279 1671
Size: 1279 392
BsaHI Gr'CG_yC
Cuts at: 0 563 584 1016 1223 1671
Size: 563 21 432 207 448
Fragments arranged by size:
563 448 432 207 21
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1651 1671
Size: 1651 20
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 439 1671
Size: 439 1232
BseYI C'CCAG_C
Cuts at: 0 109 123 315 1671
Size: 109 14 192 1356
Fragments arranged by size:
1356 192 109 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1159 1240 1422 1591 1671
Size: 1111 48 81 182 169 80
Fragments arranged by size:
1111 182 169 81 80 48
BsiEI CG_ry'CG
Cuts at: 0 539 631 658 752 1048 1052 1199 1671
Size: 539 92 27 94 296 4 147 472
Fragments arranged by size:
539 472 296 147 94 92 27 4
BsiHKAI G_wGCw'C
Cuts at: 0 25 614 902 1513 1671
Size: 25 589 288 611 158
Fragments arranged by size:
611 589 288 158 25
BsmI GAATG_Cn'
Cuts at: 0 454 1671
Size: 454 1217
Bsp1286I G_dGCh'C
Cuts at: 0 25 49 122 163 309 555 614 902
Size: 25 24 73 41 146 246 59 288
Cuts at: 902 1118 1124 1365 1513 1671
Size: 216 6 241 148 158
Fragments arranged by size:
288 246 241 216 158 148 146 73
59 41 25 24 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 836 1064 1671
Size: 836 228 607
Fragments arranged by size:
836 607 228
BsrBI CCG'CTC
Cuts at: 0 302 546 1308 1671
Size: 302 244 762 363
Fragments arranged by size:
762 363 302 244
BsrFI r'CCGG_y
Cuts at: 0 747 754 807 850 935 1047 1201 1671
Size: 747 7 53 43 85 112 154 470
Fragments arranged by size:
747 470 154 112 85 53 43 7
BssHII G'CGCG_C
Cuts at: 0 685 800 846 854 889 915 917 989
Size: 685 115 46 8 35 26 2 72
Cuts at: 989 1001 1671
Size: 12 670
Fragments arranged by size:
685 670 115 72 46 35 26 12
8 2
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1517 1671
Size: 1517 154
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1299 1365 1671
Size: 1299 66 306
Fragments arranged by size:
1299 306 66
BstYI r'GATC_y
Cuts at: 0 167 1274 1671
Size: 167 1107 397
Fragments arranged by size:
1107 397 167
Bsu36I CC'TnA_GG
Cuts at: 0 422 1070 1671
Size: 422 648 601
Fragments arranged by size:
648 601 422
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 435 1517 1671
Size: 435 1082 154
Fragments arranged by size:
1082 435 154
BtsI GCAGTG_nn'
Cuts at: 0 107 295 1671
Size: 107 188 1376
Fragments arranged by size:
1376 188 107
DraIII CAC_nnn'GTG
Cuts at: 0 1025 1533 1671
Size: 1025 508 138
Fragments arranged by size:
1025 508 138
EaeI y'GGCC_r
Cuts at: 0 536 628 655 749 950 1045 1049 1196
Size: 536 92 27 94 201 95 4 147
Cuts at: 1196 1301 1557 1671
Size: 105 256 114
Fragments arranged by size:
536 256 201 147 114 105 95 94
92 27 4
EagI C'GGCC_G
Cuts at: 0 536 628 655 749 1045 1049 1196 1671
Size: 536 92 27 94 296 4 147 475
Fragments arranged by size:
536 475 296 147 94 92 27 4
EarI CTCTTCn'nnn_
Cuts at: 0 145 1252 1671
Size: 145 1107 419
Fragments arranged by size:
1107 419 145
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 691 1671
Size: 691 980
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 292 318 504 1047 1671
Size: 216 76 26 186 543 624
Fragments arranged by size:
624 543 216 186 76 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1597 1671
Size: 1597 74
EcoO109I rG'GnC_Cy
Cuts at: 0 45 46 305 306 333 552 1074 1121
Size: 45 1 259 1 27 219 522 47
Cuts at: 1121 1671
Size: 550
Fragments arranged by size:
550 522 259 219 47 45 27 1
1
EcoRI G'AATT_C
Cuts at: 0 1093 1671
Size: 1093 578
FseI GG_CCGG'CC
Cuts at: 0 1051 1671
Size: 1051 620
HaeII r_GCGC'y
Cuts at: 0 418 1671
Size: 418 1253
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 1470 1502 1671
Size: 373 32 1065 32 169
Fragments arranged by size:
1065 373 169 32 32
HincII GTy'rAC
Cuts at: 0 1158 1671
Size: 1158 513
MscI TGG'CCA
Cuts at: 0 1559 1671
Size: 1559 112
MspA1I CmG'CkG
Cuts at: 0 113 135 524 591 1133 1212 1671
Size: 113 22 389 67 542 79 459
Fragments arranged by size:
542 459 389 113 79 67 22
NaeI GCC'GGC
Cuts at: 0 749 756 809 852 937 1049 1203 1671
Size: 749 7 53 43 85 112 154 468
Fragments arranged by size:
749 468 154 112 85 53 43 7
NgoMIV G'CCGG_C
Cuts at: 0 747 754 807 850 935 1047 1201 1671
Size: 747 7 53 43 85 112 154 470
Fragments arranged by size:
747 470 154 112 85 53 43 7
NotI GC'GGCC_GC
Cuts at: 0 628 1196 1671
Size: 628 568 475
Fragments arranged by size:
628 568 475
NspI r_CATG'y
Cuts at: 0 1209 1556 1566 1671
Size: 1209 347 10 105
Fragments arranged by size:
1209 347 105 10
PasI CC'CwG_GG
Cuts at: 0 1365 1671
Size: 1365 306
PciI A'CATG_T
Cuts at: 0 1552 1562 1671
Size: 1552 10 109
Fragments arranged by size:
1552 109 10
PfoI T'CCnGG_A
Cuts at: 0 614 1671
Size: 614 1057
PmlI CAC'GTG
Cuts at: 0 1025 1400 1671
Size: 1025 375 271
Fragments arranged by size:
1025 375 271
PpuMI rG'GwC_Cy
Cuts at: 0 333 1671
Size: 333 1338
PspOMI G'GGCC_C
Cuts at: 0 45 305 551 1120 1671
Size: 45 260 246 569 551
Fragments arranged by size:
569 551 260 246 45
PvuII CAG'CTG
Cuts at: 0 591 1212 1671
Size: 591 621 459
Fragments arranged by size:
621 591 459
SapI GCTCTTCn'nnn_
Cuts at: 0 145 1252 1671
Size: 145 1107 419
Fragments arranged by size:
1107 419 145
SexAI A'CCwGG_T
Cuts at: 0 383 1671
Size: 383 1288
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 748 1671
Size: 748 923
SgrAI Cr'CCGG_yG
Cuts at: 0 850 1671
Size: 850 821
SmaI CCC'GGG
Cuts at: 0 1119 1671
Size: 1119 552
SmlI C'TyrA_G
Cuts at: 0 1594 1671
Size: 1594 77
SphI G_CATG'C
Cuts at: 0 1209 1671
Size: 1209 462
SrfI GCCC'GGGC
Cuts at: 0 1119 1671
Size: 1119 552
StyI C'CwwG_G
Cuts at: 0 155 410 1671
Size: 155 255 1261
Fragments arranged by size:
1261 255 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 450 1671
Size: 450 1221
Tth111I GACn'n_nGTC
Cuts at: 0 1540 1671
Size: 1540 131
XmaI C'CCGG_G
Cuts at: 0 1117 1671
Size: 1117 554
ZraI GAC'GTC
Cuts at: 0 564 1671
Size: 564 1107
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BbvCI BcgI BciVI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstYI Bsu36I BtgZI BtsI DraIII EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI HaeII
Hin4I HincII MscI MspA1I NaeI NgoMIV NotI NspI
PasI PciI PfoI PmlI PpuMI PspOMI PvuII SapI
SexAI SfiI SgrAI SmaI SmlI SphI SrfI StyI
TaqII Tth111I XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BclI BfrBI BglII BmtI BplI BsaWI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstZ17I BtgI ClaI CspCI DraI DrdI EcoICRI EcoRV
FalI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NruI
NsiI PacI PflMI PmeI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SnaBI SpeI SspI
StuI SwaI TatI TstI XbaI XcmI XhoI XmnI