Restriction map for
pFN21AB5054
(Linear) (Six-base) MAPSORT of: KIBB5054.seq Check: 6255 from: 1 to: 1593
KIBB5054.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
AccI GT'mk_AC
Cuts at: 0 1057 1593
Size: 1057 536
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 353 376 380 1360 1593
Size: 353 23 4 980 233
Fragments arranged by size:
980 353 233 23 4
AflIII A'CryG_T
Cuts at: 0 701 1593
Size: 701 892
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 202 1593
Size: 202 1391
AlwNI CAG_nnn'CTG
Cuts at: 0 511 1231 1593
Size: 511 720 362
Fragments arranged by size:
720 511 362
ApaI G_GGCC'C
Cuts at: 0 119 1593
Size: 119 1474
ApoI r'AATT_y
Cuts at: 0 203 732 793 999 1394 1593
Size: 203 529 61 206 395 199
Fragments arranged by size:
529 395 206 203 199 61
AseI AT'TA_AT
Cuts at: 0 875 1593
Size: 875 718
AvaI C'yCGr_G
Cuts at: 0 41 1593
Size: 41 1552
BanI G'GyrC_C
Cuts at: 0 124 1234 1593
Size: 124 1110 359
Fragments arranged by size:
1110 359 124
BanII G_rGCy'C
Cuts at: 0 119 1593
Size: 119 1474
BbsI GAAGACnn'nnnn_
Cuts at: 0 19 1571 1593
Size: 19 1552 22
Fragments arranged by size:
1552 22 19
BclI T'GATC_A
Cuts at: 0 1124 1593
Size: 1124 469
BfrBI ATG'CAT
Cuts at: 0 682 686 1327 1593
Size: 682 4 641 266
Fragments arranged by size:
682 641 266 4
BglI GCCn_nnn'nGGC
Cuts at: 0 37 1593
Size: 37 1556
Bme1580I G_kGCm'C
Cuts at: 0 119 127 1299 1593
Size: 119 8 1172 294
Fragments arranged by size:
1172 294 119 8
BmgBI CAC'GTC
Cuts at: 0 96 1593
Size: 96 1497
BmrI ACTGGGnnnn_n'
Cuts at: 0 1274 1593
Size: 1274 319
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 790 989 1593
Size: 790 199 604
Fragments arranged by size:
790 604 199
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 1593
Size: 173 1420
Bpu10I CC'TnA_GC
Cuts at: 0 100 1593
Size: 100 1493
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 1593
Size: 1203 390
BsaBI GATnn'nnATC
Cuts at: 0 574 1593
Size: 574 1019
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1506 1593
Size: 776 730 87
Fragments arranged by size:
776 730 87
BsiHKAI G_wGCw'C
Cuts at: 0 648 1593
Size: 648 945
Bsp1286I G_dGCh'C
Cuts at: 0 119 127 648 1299 1593
Size: 119 8 521 651 294
Fragments arranged by size:
651 521 294 119 8
BspHI T'CATG_A
Cuts at: 0 566 1593
Size: 566 1027
BsrDI GCAATG_nn'
Cuts at: 0 186 1256 1327 1593
Size: 186 1070 71 266
Fragments arranged by size:
1070 266 186 71
BssSI C'ACGA_G
Cuts at: 0 775 1593
Size: 775 818
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 1593
Size: 96 1497
BstYI r'GATC_y
Cuts at: 0 318 1593
Size: 318 1275
BstZ17I GTA'TAC
Cuts at: 0 1058 1593
Size: 1058 535
BtgI C'CryG_G
Cuts at: 0 639 1238 1593
Size: 639 599 355
Fragments arranged by size:
639 599 355
BtsI GCAGTG_nn'
Cuts at: 0 1175 1593
Size: 1175 418
EaeI y'GGCC_r
Cuts at: 0 452 530 1593
Size: 452 78 1063
Fragments arranged by size:
1063 452 78
EarI CTCTTCn'nnn_
Cuts at: 0 470 568 755 1064 1593
Size: 470 98 187 309 529
Fragments arranged by size:
529 470 309 187 98
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 331 1593
Size: 331 1262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 353 376 380 1360 1593
Size: 173 180 23 4 980 233
Fragments arranged by size:
980 233 180 173 23 4
EcoO109I rG'GnC_Cy
Cuts at: 0 115 387 1593
Size: 115 272 1206
Fragments arranged by size:
1206 272 115
EcoRI G'AATT_C
Cuts at: 0 732 999 1593
Size: 732 267 594
Fragments arranged by size:
732 594 267
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1098 1593
Size: 1098 495
FspI TGC'GCA
Cuts at: 0 940 1593
Size: 940 653
HaeII r_GCGC'y
Cuts at: 0 58 152 1593
Size: 58 94 1441
Fragments arranged by size:
1441 94 58
HincII GTy'rAC
Cuts at: 0 66 1593
Size: 66 1527
HindIII A'AGCT_T
Cuts at: 0 612 1593
Size: 612 981
MscI TGG'CCA
Cuts at: 0 454 532 1593
Size: 454 78 1061
Fragments arranged by size:
1061 454 78
MslI CAynn'nnrTG
Cuts at: 0 133 583 1593
Size: 133 450 1010
Fragments arranged by size:
1010 450 133
NsiI A_TGCA'T
Cuts at: 0 684 688 1329 1593
Size: 684 4 641 264
Fragments arranged by size:
684 641 264 4
NspI r_CATG'y
Cuts at: 0 686 1039 1593
Size: 686 353 554
Fragments arranged by size:
686 554 353
PflMI CCAn_nnn'nTGG
Cuts at: 0 1231 1593
Size: 1231 362
PshAI GACnn'nnGTC
Cuts at: 0 1016 1593
Size: 1016 577
PspOMI G'GGCC_C
Cuts at: 0 115 1593
Size: 115 1478
PstI C_TGCA'G
Cuts at: 0 92 1593
Size: 92 1501
SfcI C'TryA_G
Cuts at: 0 88 512 1593
Size: 88 424 1081
Fragments arranged by size:
1081 424 88
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 37 1593
Size: 37 1556
SmlI C'TyrA_G
Cuts at: 0 1182 1593
Size: 1182 411
SphI G_CATG'C
Cuts at: 0 686 1593
Size: 686 907
StuI AGG'CCT
Cuts at: 0 338 1593
Size: 338 1255
StyI C'CwwG_G
Cuts at: 0 26 119 1343 1593
Size: 26 93 1224 250
Fragments arranged by size:
1224 250 93 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 227 1420 1593
Size: 227 1193 173
Fragments arranged by size:
1193 227 173
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 828 860 1593
Size: 828 32 733
Fragments arranged by size:
828 733 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 1545 1593
Size: 33 1512 48
Fragments arranged by size:
1512 48 33
XmnI GAAnn'nnTTC
Cuts at: 0 338 1578 1593
Size: 338 1240 15
Fragments arranged by size:
1240 338 15
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApoI AseI
AvaI BanI BanII BbsI BclI BfrBI BglI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaBI BsgI
BsiHKAI Bsp1286I BspHI BsrDI BssSI BstAPI BstYI BstZ17I
BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI HaeII HincII HindIII MscI MslI NsiI
NspI PflMI PshAI PspOMI PstI SfcI SfiI SmlI
SphI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BglII BlpI BmtI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BstBI BstEII BstXI Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoNI EcoRV FseI FspAI
Hin4I HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI
PsiI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI ZraI