Restriction map for
pFN21AB4905
(Linear) (Six-base) MAPSORT of: KIBB4905.seq Check: 2774 from: 1 to: 1056
KIBB4905.orf
With 182 enzymes: SgfI *
July 15, 2010 18:29 ..
Acc65I G'GTAC_C
Cuts at: 0 512 1056
Size: 512 544
AfeI AGC'GCT
Cuts at: 0 148 1056
Size: 148 908
AflIII A'CryG_T
Cuts at: 0 854 1056
Size: 854 202
AleI CACnn'nnGTG
Cuts at: 0 472 1056
Size: 472 584
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 490 1056
Size: 490 566
BanI G'GyrC_C
Cuts at: 0 241 512 527 1056
Size: 241 271 15 529
Fragments arranged by size:
529 271 241 15
BanII G_rGCy'C
Cuts at: 0 773 928 1001 1056
Size: 773 155 73 55
Fragments arranged by size:
773 155 73 55
BbvCI CC'TCA_GC
Cuts at: 0 728 1056
Size: 728 328
BciVI GTATCCnnnnn_n'
Cuts at: 0 136 1056
Size: 136 920
BglI GCCn_nnn'nGGC
Cuts at: 0 193 656 754 953 1056
Size: 193 463 98 199 103
Fragments arranged by size:
463 199 193 103 98
BlpI GC'TnA_GC
Cuts at: 0 787 1056
Size: 787 269
Bme1580I G_kGCm'C
Cuts at: 0 479 530 922 1056
Size: 479 51 392 134
Fragments arranged by size:
479 392 134 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 66 1056
Size: 66 990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 934 1011 1015 1056
Size: 267 667 77 4 41
Fragments arranged by size:
667 267 77 41 4
Bpu10I CC'TnA_GC
Cuts at: 0 728 826 1056
Size: 728 98 230
Fragments arranged by size:
728 230 98
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1021 1056
Size: 1021 35
BsaAI yAC'GTr
Cuts at: 0 714 1056
Size: 714 342
BsaHI Gr'CG_yC
Cuts at: 0 615 934 1056
Size: 615 319 122
Fragments arranged by size:
615 319 122
BseYI C'CCAG_C
Cuts at: 0 517 1056
Size: 517 539
BsiEI CG_ry'CG
Cuts at: 0 660 1056
Size: 660 396
BsiHKAI G_wGCw'C
Cuts at: 0 674 809 1056
Size: 674 135 247
Fragments arranged by size:
674 247 135
Bsp1286I G_dGCh'C
Cuts at: 0 479 530 674 773 809 922 928 1001
Size: 479 51 144 99 36 113 6 73
Cuts at: 1001 1056
Size: 55
Fragments arranged by size:
479 144 113 99 73 55 51 36
6
BsrBI CCG'CTC
Cuts at: 0 344 980 991 1056
Size: 344 636 11 65
Fragments arranged by size:
636 344 65 11
BsrFI r'CCGG_y
Cuts at: 0 655 748 862 1056
Size: 655 93 114 194
Fragments arranged by size:
655 194 114 93
BssHII G'CGCG_C
Cuts at: 0 740 1056
Size: 740 316
BssSI C'ACGA_G
Cuts at: 0 772 1056
Size: 772 284
BstXI CCAn_nnnn'nTGG
Cuts at: 0 396 912 1056
Size: 396 516 144
Fragments arranged by size:
516 396 144
BtgI C'CryG_G
Cuts at: 0 508 948 1056
Size: 508 440 108
Fragments arranged by size:
508 440 108
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 409 795 1056
Size: 409 386 261
Fragments arranged by size:
409 386 261
BtsI GCAGTG_nn'
Cuts at: 0 155 206 1019 1056
Size: 155 51 813 37
Fragments arranged by size:
813 155 51 37
DraIII CAC_nnn'GTG
Cuts at: 0 37 717 1056
Size: 37 680 339
Fragments arranged by size:
680 339 37
EaeI y'GGCC_r
Cuts at: 0 185 194 326 443 593 657 746 750
Size: 185 9 132 117 150 64 89 4
Cuts at: 750 1056
Size: 306
Fragments arranged by size:
306 185 150 132 117 89 64 9
4
EagI C'GGCC_G
Cuts at: 0 657 1056
Size: 657 399
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 628 1056
Size: 628 428
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 934 1011 1015 1056
Size: 267 667 77 4 41
Fragments arranged by size:
667 267 77 41 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 727 1056
Size: 727 329
FseI GG_CCGG'CC
Cuts at: 0 752 1056
Size: 752 304
HaeII r_GCGC'y
Cuts at: 0 85 150 636 1056
Size: 85 65 486 420
Fragments arranged by size:
486 420 85 65
KpnI G_GTAC'C
Cuts at: 0 516 1056
Size: 516 540
MluI A'CGCG_T
Cuts at: 0 854 1056
Size: 854 202
MscI TGG'CCA
Cuts at: 0 445 595 1056
Size: 445 150 461
Fragments arranged by size:
461 445 150
MslI CAynn'nnrTG
Cuts at: 0 328 424 472 1056
Size: 328 96 48 584
Fragments arranged by size:
584 328 96 48
MspA1I CmG'CkG
Cuts at: 0 510 950 1056
Size: 510 440 106
Fragments arranged by size:
510 440 106
NaeI GCC'GGC
Cuts at: 0 750 864 1056
Size: 750 114 192
Fragments arranged by size:
750 192 114
NgoMIV G'CCGG_C
Cuts at: 0 748 862 1056
Size: 748 114 194
Fragments arranged by size:
748 194 114
NspI r_CATG'y
Cuts at: 0 303 1056
Size: 303 753
PmlI CAC'GTG
Cuts at: 0 714 1056
Size: 714 342
PstI C_TGCA'G
Cuts at: 0 21 1056
Size: 21 1035
SacII CC_GC'GG
Cuts at: 0 511 951 1056
Size: 511 440 105
Fragments arranged by size:
511 440 105
SfcI C'TryA_G
Cuts at: 0 17 622 1056
Size: 17 605 434
Fragments arranged by size:
605 434 17
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 193 754 1056
Size: 193 561 302
Fragments arranged by size:
561 302 193
SgrAI Cr'CCGG_yG
Cuts at: 0 862 1056
Size: 862 194
SmlI C'TyrA_G
Cuts at: 0 1036 1056
Size: 1036 20
StyI C'CwwG_G
Cuts at: 0 237 389 1056
Size: 237 152 667
Fragments arranged by size:
667 237 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 1056
Size: 414 642
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 806 838 1056
Size: 806 32 218
Fragments arranged by size:
806 218 32
XbaI T'CTAG_A
Cuts at: 0 336 1056
Size: 336 720
Enzymes that do cut:
Acc65I AfeI AflIII AleI AlfI BanI BanII BbvCI
BciVI BglI BlpI Bme1580I BplI BpmI Bpu10I BpuEI
BsaAI BsaHI BseYI BsiEI BsiHKAI Bsp1286I BsrBI BsrFI
BssHII BssSI BstXI BtgI BtgZI BtsI DraIII EaeI
EagI EciI Eco57MI EcoNI FseI HaeII KpnI MluI
MscI MslI MspA1I NaeI NgoMIV NspI PmlI PstI
SacII SfcI SfiI SgrAI SmlI StyI TaqII TstI
XbaI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BbeI BbsI BcgI BclI
BfrBI BglII BmgBI BmrI BmtI BsaI BsaBI BsaWI
BsaXI BseRI BsgI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I ClaI CspCI DraI DrdI EarI EcoICRI EcoO109I
EcoRI EcoRV FalI FspI FspAI Hin4I HincII HindIII
HpaI KasI MfeI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SexAI SfoI SgfI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI Tth111I XcmI XhoI
XmaI XmnI ZraI