Restriction map for
pFN21AB4810
(Linear) (Six-base) MAPSORT of: KIBB4810.seq Check: 7929 from: 1 to: 915
KIBB4810.orf
With 182 enzymes: SgfI *
January 17, 2012 13:59 ..
AccI GT'mk_AC
Cuts at: 0 512 915
Size: 512 403
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 915
Size: 812 103
AleI CACnn'nnGTG
Cuts at: 0 520 670 915
Size: 520 150 245
Fragments arranged by size:
520 245 150
AlwNI CAG_nnn'CTG
Cuts at: 0 200 915
Size: 200 715
ApoI r'AATT_y
Cuts at: 0 231 546 832 915
Size: 231 315 286 83
Fragments arranged by size:
315 286 231 83
BanII G_rGCy'C
Cuts at: 0 401 915
Size: 401 514
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 839 915
Size: 116 723 76
Fragments arranged by size:
723 116 76
BmgBI CAC'GTC
Cuts at: 0 522 915
Size: 522 393
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 639 915
Size: 639 276
BsaBI GATnn'nnATC
Cuts at: 0 175 915
Size: 175 740
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 25 357 811 915
Size: 22 3 332 454 104
Fragments arranged by size:
454 332 104 22 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 445 915
Size: 445 470
BsiHKAI G_wGCw'C
Cuts at: 0 685 915
Size: 685 230
BsmI GAATG_Cn'
Cuts at: 0 123 915
Size: 123 792
BsmBI CGTCTCn'nnnn_
Cuts at: 0 368 915
Size: 368 547
Bsp1286I G_dGCh'C
Cuts at: 0 401 685 915
Size: 401 284 230
Fragments arranged by size:
401 284 230
BsrFI r'CCGG_y
Cuts at: 0 574 915
Size: 574 341
BssSI C'ACGA_G
Cuts at: 0 166 915
Size: 166 749
BstXI CCAn_nnnn'nTGG
Cuts at: 0 213 915
Size: 213 702
BstYI r'GATC_y
Cuts at: 0 65 197 915
Size: 65 132 718
Fragments arranged by size:
718 132 65
BtgI C'CryG_G
Cuts at: 0 122 668 915
Size: 122 546 247
Fragments arranged by size:
546 247 122
BtsI GCAGTG_nn'
Cuts at: 0 19 428 915
Size: 19 409 487
Fragments arranged by size:
487 409 19
ClaI AT'CG_AT
Cuts at: 0 494 915
Size: 494 421
DraI TTT'AAA
Cuts at: 0 837 915
Size: 837 78
EaeI y'GGCC_r
Cuts at: 0 56 915
Size: 56 859
EarI CTCTTCn'nnn_
Cuts at: 0 334 673 788 915
Size: 334 339 115 127
Fragments arranged by size:
339 334 127 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 556 915
Size: 556 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 915
Size: 812 103
EcoO109I rG'GnC_Cy
Cuts at: 0 86 801 854 915
Size: 86 715 53 61
Fragments arranged by size:
715 86 61 53
HaeII r_GCGC'y
Cuts at: 0 53 915
Size: 53 862
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 255 287 333 365 710 742 915
Size: 255 32 46 32 345 32 173
Fragments arranged by size:
345 255 173 46 32 32 32
HindIII A'AGCT_T
Cuts at: 0 776 915
Size: 776 139
MscI TGG'CCA
Cuts at: 0 58 915
Size: 58 857
MslI CAynn'nnrTG
Cuts at: 0 166 211 356 520 556 670 915
Size: 166 45 145 164 36 114 245
Fragments arranged by size:
245 166 164 145 114 45 36
MspA1I CmG'CkG
Cuts at: 0 533 915
Size: 533 382
NaeI GCC'GGC
Cuts at: 0 576 915
Size: 576 339
NcoI C'CATG_G
Cuts at: 0 122 915
Size: 122 793
NdeI CA'TA_TG
Cuts at: 0 696 915
Size: 696 219
NgoMIV G'CCGG_C
Cuts at: 0 574 915
Size: 574 341
PpuMI rG'GwC_Cy
Cuts at: 0 86 915
Size: 86 829
PstI C_TGCA'G
Cuts at: 0 583 915
Size: 583 332
SapI GCTCTTCn'nnn_
Cuts at: 0 673 915
Size: 673 242
SfcI C'TryA_G
Cuts at: 0 579 841 915
Size: 579 262 74
Fragments arranged by size:
579 262 74
SmlI C'TyrA_G
Cuts at: 0 618 915
Size: 618 297
StuI AGG'CCT
Cuts at: 0 601 915
Size: 601 314
StyI C'CwwG_G
Cuts at: 0 122 915
Size: 122 793
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 550 915
Size: 550 365
Enzymes that do cut:
AccI AcuI AleI AlwNI ApoI BanII BbsI BmgBI
BpuEI BsaBI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BsrFI BssSI BstXI BstYI BtgI BtsI ClaI DraI
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HindIII
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV PpuMI
PstI SapI SfcI SmlI StuI StyI TaqII
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BspEI BspHI BspMI BsrBI
BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI CspCI DraIII DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI NarI NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI