Restriction map for
pFN21AB4605
(Linear) (Six-base) MAPSORT of: KIBB4605.seq Check: 840 from: 1 to: 1416
KIBB4605.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 737 1178 1416
Size: 565 172 441 238
Fragments arranged by size:
565 441 238 172
AflII C'TTAA_G
Cuts at: 0 820 1416
Size: 820 596
AflIII A'CryG_T
Cuts at: 0 384 1416
Size: 384 1032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 638 1007 1416
Size: 638 369 409
Fragments arranged by size:
638 409 369
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1210 1242 1416
Size: 1210 32 174
Fragments arranged by size:
1210 174 32
ApaLI G'TGCA_C
Cuts at: 0 1299 1416
Size: 1299 117
ApoI r'AATT_y
Cuts at: 0 725 1115 1416
Size: 725 390 301
Fragments arranged by size:
725 390 301
BamHI G'GATC_C
Cuts at: 0 1225 1416
Size: 1225 191
BanII G_rGCy'C
Cuts at: 0 657 761 1416
Size: 657 104 655
Fragments arranged by size:
657 655 104
BbvCI CC'TCA_GC
Cuts at: 0 497 1416
Size: 497 919
BciVI GTATCCnnnnn_n'
Cuts at: 0 856 1416
Size: 856 560
BglII A'GATC_T
Cuts at: 0 307 778 1416
Size: 307 471 638
Fragments arranged by size:
638 471 307
BlpI GC'TnA_GC
Cuts at: 0 677 1416
Size: 677 739
Bme1580I G_kGCm'C
Cuts at: 0 1303 1416
Size: 1303 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 206 980 1416
Size: 38 168 774 436
Fragments arranged by size:
774 436 168 38
Bpu10I CC'TnA_GC
Cuts at: 0 497 1327 1416
Size: 497 830 89
Fragments arranged by size:
830 497 89
BsaHI Gr'CG_yC
Cuts at: 0 85 1416
Size: 85 1331
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1232 1236 1262 1266 1416
Size: 1232 4 26 4 150
Fragments arranged by size:
1232 150 26 4 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1397 1416
Size: 1397 19
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 464 1097 1416
Size: 464 633 319
Fragments arranged by size:
633 464 319
BsiHKAI G_wGCw'C
Cuts at: 0 657 1211 1303 1416
Size: 657 554 92 113
Fragments arranged by size:
657 554 113 92
Bsp1286I G_dGCh'C
Cuts at: 0 657 761 1211 1303 1416
Size: 657 104 450 92 113
Fragments arranged by size:
657 450 113 104 92
BspHI T'CATG_A
Cuts at: 0 536 1416
Size: 536 880
BstYI r'GATC_y
Cuts at: 0 54 307 617 778 1225 1416
Size: 54 253 310 161 447 191
Fragments arranged by size:
447 310 253 191 161 54
BtsI GCAGTG_nn'
Cuts at: 0 455 1416
Size: 455 961
DraIII CAC_nnn'GTG
Cuts at: 0 1040 1416
Size: 1040 376
EaeI y'GGCC_r
Cuts at: 0 292 408 1416
Size: 292 116 1008
Fragments arranged by size:
1008 292 116
EarI CTCTTCn'nnn_
Cuts at: 0 470 1329 1374 1416
Size: 470 859 45 42
Fragments arranged by size:
859 470 45 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 23 1416
Size: 23 1393
EcoICRI GAG'CTC
Cuts at: 0 655 1416
Size: 655 761
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 206 565 737 980 1178 1416
Size: 38 168 359 172 243 198 238
Fragments arranged by size:
359 243 238 198 172 168 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1376 1408 1416
Size: 1376 32 8
Fragments arranged by size:
1376 32 8
HincII GTy'rAC
Cuts at: 0 83 399 792 1235 1416
Size: 83 316 393 443 181
Fragments arranged by size:
443 393 316 181 83
HindIII A'AGCT_T
Cuts at: 0 238 268 1416
Size: 238 30 1148
Fragments arranged by size:
1148 238 30
MfeI C'AATT_G
Cuts at: 0 137 1416
Size: 137 1279
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1062 1416
Size: 996 66 354
Fragments arranged by size:
996 354 66
MscI TGG'CCA
Cuts at: 0 410 1416
Size: 410 1006
MspA1I CmG'CkG
Cuts at: 0 681 1416
Size: 681 735
NspI r_CATG'y
Cuts at: 0 388 1416
Size: 388 1028
PciI A'CATG_T
Cuts at: 0 384 1416
Size: 384 1032
PsiI TTA'TAA
Cuts at: 0 266 1416
Size: 266 1150
PvuII CAG'CTG
Cuts at: 0 681 1416
Size: 681 735
SacI G_AGCT'C
Cuts at: 0 657 1416
Size: 657 759
SapI GCTCTTCn'nnn_
Cuts at: 0 470 1416
Size: 470 946
ScaI AGT'ACT
Cuts at: 0 1070 1416
Size: 1070 346
SexAI A'CCwGG_T
Cuts at: 0 891 1416
Size: 891 525
SfcI C'TryA_G
Cuts at: 0 527 1416
Size: 527 889
SmlI C'TyrA_G
Cuts at: 0 820 1416
Size: 820 596
SspI AAT'ATT
Cuts at: 0 228 693 1416
Size: 228 465 723
Fragments arranged by size:
723 465 228
StyI C'CwwG_G
Cuts at: 0 65 633 1416
Size: 65 568 783
Fragments arranged by size:
783 568 65
TatI w'GTAC_w
Cuts at: 0 322 710 1068 1416
Size: 322 388 358 348
Fragments arranged by size:
388 358 348 322
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1236 1268 1416
Size: 1236 32 148
Fragments arranged by size:
1236 148 32
Enzymes that do cut:
AcuI AflII AflIII AlfI AloI ApaLI ApoI BamHI
BanII BbvCI BciVI BglII BlpI Bme1580I BpmI Bpu10I
BsaHI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI BstYI
BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI Hin4I
HincII HindIII MfeI MmeI MscI MspA1I NspI PciI
PsiI PvuII SacI SapI ScaI SexAI SfcI SmlI
SspI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlwNI ApaI AscI AseI AsiSI AvaI AvrII
BaeI BanI BbeI BbsI BcgI BclI BfrBI BglI
BmgBI BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HpaI KasI KpnI MluI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmaI XmnI ZraI