Restriction map for
pFN21AB4483
(Linear) (Six-base) MAPSORT of: KIBB4483.seq Check: 557 from: 1 to: 1599
KIBB4483.orf
With 182 enzymes: SgfI *
June 25, 2013 10:30 ..
AatII G_ACGT'C
Cuts at: 0 1436 1599
Size: 1436 163
AccI GT'mk_AC
Cuts at: 0 524 1599
Size: 524 1075
Acc65I G'GTAC_C
Cuts at: 0 1508 1599
Size: 1508 91
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 484 1480 1599
Size: 484 996 119
Fragments arranged by size:
996 484 119
AfeI AGC'GCT
Cuts at: 0 828 1599
Size: 828 771
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 499 1599
Size: 499 1100
AlwNI CAG_nnn'CTG
Cuts at: 0 511 1197 1599
Size: 511 686 402
Fragments arranged by size:
686 511 402
ApaI G_GGCC'C
Cuts at: 0 21 1599
Size: 21 1578
ApoI r'AATT_y
Cuts at: 0 1384 1599
Size: 1384 215
AvaI C'yCGr_G
Cuts at: 0 80 145 1225 1599
Size: 80 65 1080 374
Fragments arranged by size:
1080 374 80 65
AvrII C'CTAG_G
Cuts at: 0 549 1599
Size: 549 1050
BanI G'GyrC_C
Cuts at: 0 120 483 553 1213 1508 1599
Size: 120 363 70 660 295 91
Fragments arranged by size:
660 363 295 120 91 70
BanII G_rGCy'C
Cuts at: 0 21 65 173 813 882 1211 1268 1599
Size: 21 44 108 640 69 329 57 331
Fragments arranged by size:
640 331 329 108 69 57 44 21
BbeI G_GCGC'C
Cuts at: 0 124 1217 1599
Size: 124 1093 382
Fragments arranged by size:
1093 382 124
BbsI GAAGACnn'nnnn_
Cuts at: 0 1071 1599
Size: 1071 528
BbvCI CC'TCA_GC
Cuts at: 0 331 1363 1599
Size: 331 1032 236
Fragments arranged by size:
1032 331 236
BciVI GTATCCnnnnn_n'
Cuts at: 0 904 1599
Size: 904 695
BfrBI ATG'CAT
Cuts at: 0 1450 1599
Size: 1450 149
BglII A'GATC_T
Cuts at: 0 156 612 1163 1599
Size: 156 456 551 436
Fragments arranged by size:
551 456 436 156
BlpI GC'TnA_GC
Cuts at: 0 493 1198 1599
Size: 493 705 401
Fragments arranged by size:
705 493 401
Bme1580I G_kGCm'C
Cuts at: 0 21 1599
Size: 21 1578
BmgBI CAC'GTC
Cuts at: 0 564 1116 1599
Size: 564 552 483
Fragments arranged by size:
564 552 483
BmrI ACTGGGnnnn_n'
Cuts at: 0 807 1599
Size: 807 792
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 607 1599
Size: 607 992
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 602 617 1120 1141 1599
Size: 418 184 15 503 21 458
Fragments arranged by size:
503 458 418 184 21 15
Bpu10I CC'TnA_GC
Cuts at: 0 167 331 1363 1599
Size: 167 164 1032 236
Fragments arranged by size:
1032 236 167 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1019 1599
Size: 1019 580
BsaI GGTCTCn'nnnn_
Cuts at: 0 800 1599
Size: 800 799
BsaAI yAC'GTr
Cuts at: 0 972 1053 1599
Size: 972 81 546
Fragments arranged by size:
972 546 81
BsaHI Gr'CG_yC
Cuts at: 0 121 1129 1214 1433 1599
Size: 121 1008 85 219 166
Fragments arranged by size:
1008 219 166 121 85
BsaWI w'CCGG_w
Cuts at: 0 713 1599
Size: 713 886
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 396 1599
Size: 396 1203
BseYI C'CCAG_C
Cuts at: 0 6 57 393 1599
Size: 6 51 336 1206
Fragments arranged by size:
1206 336 51 6
BsiHKAI G_wGCw'C
Cuts at: 0 79 1268 1599
Size: 79 1189 331
Fragments arranged by size:
1189 331 79
Bsp1286I G_dGCh'C
Cuts at: 0 21 65 79 173 813 882 1211 1268
Size: 21 44 14 94 640 69 329 57
Cuts at: 1268 1599
Size: 331
Fragments arranged by size:
640 331 329 94 69 57 44 21
14
BsrBI CCG'CTC
Cuts at: 0 432 1125 1599
Size: 432 693 474
Fragments arranged by size:
693 474 432
BsrFI r'CCGG_y
Cuts at: 0 1553 1574 1599
Size: 1553 21 25
Fragments arranged by size:
1553 25 21
BssSI C'ACGA_G
Cuts at: 0 1261 1599
Size: 1261 338
BstEII G'GTnAC_C
Cuts at: 0 915 1290 1599
Size: 915 375 309
Fragments arranged by size:
915 375 309
BstYI r'GATC_y
Cuts at: 0 156 612 1163 1599
Size: 156 456 551 436
Fragments arranged by size:
551 456 436 156
BstZ17I GTA'TAC
Cuts at: 0 525 1599
Size: 525 1074
Bsu36I CC'TnA_GG
Cuts at: 0 469 1599
Size: 469 1130
BtgI C'CryG_G
Cuts at: 0 115 920 1599
Size: 115 805 679
Fragments arranged by size:
805 679 115
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 912 1599
Size: 912 687
BtsI GCAGTG_nn'
Cuts at: 0 1588 1599
Size: 1588 11
DrdI GACnn_nn'nnGTC
Cuts at: 0 1075 1599
Size: 1075 524
EaeI y'GGCC_r
Cuts at: 0 1025 1544 1599
Size: 1025 519 55
Fragments arranged by size:
1025 519 55
EarI CTCTTCn'nnn_
Cuts at: 0 1567 1599
Size: 1567 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 886 1599
Size: 886 713
EcoICRI GAG'CTC
Cuts at: 0 1266 1599
Size: 1266 333
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 484 602 617 1120 1141 1480 1599
Size: 418 66 118 15 503 21 339 119
Fragments arranged by size:
503 418 339 119 118 66 21 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 307 1599
Size: 307 1292
EcoO109I rG'GnC_Cy
Cuts at: 0 18 261 1599
Size: 18 243 1338
Fragments arranged by size:
1338 243 18
EcoRI G'AATT_C
Cuts at: 0 1384 1599
Size: 1384 215
HaeII r_GCGC'y
Cuts at: 0 124 830 1217 1599
Size: 124 706 387 382
Fragments arranged by size:
706 387 382 124
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1435 1467 1599
Size: 1435 32 132
Fragments arranged by size:
1435 132 32
HincII GTy'rAC
Cuts at: 0 1308 1599
Size: 1308 291
HpaI GTT'AAC
Cuts at: 0 1308 1599
Size: 1308 291
KasI G'GCGC_C
Cuts at: 0 120 1213 1599
Size: 120 1093 386
Fragments arranged by size:
1093 386 120
KpnI G_GTAC'C
Cuts at: 0 1512 1599
Size: 1512 87
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1454 1599
Size: 1454 145
MscI TGG'CCA
Cuts at: 0 1546 1599
Size: 1546 53
MslI CAynn'nnrTG
Cuts at: 0 481 1599
Size: 481 1118
MspA1I CmG'CkG
Cuts at: 0 397 405 1134 1194 1599
Size: 397 8 729 60 405
Fragments arranged by size:
729 405 397 60 8
NarI GG'CG_CC
Cuts at: 0 121 1214 1599
Size: 121 1093 385
Fragments arranged by size:
1093 385 121
NsiI A_TGCA'T
Cuts at: 0 1452 1599
Size: 1452 147
PasI CC'CwG_GG
Cuts at: 0 631 804 1599
Size: 631 173 795
Fragments arranged by size:
795 631 173
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 346 378 968 1000 1599
Size: 346 32 590 32 599
Fragments arranged by size:
599 590 346 32 32
PspOMI G'GGCC_C
Cuts at: 0 17 1599
Size: 17 1582
PstI C_TGCA'G
Cuts at: 0 241 327 1583 1599
Size: 241 86 1256 16
Fragments arranged by size:
1256 241 86 16
PvuII CAG'CTG
Cuts at: 0 397 405 1194 1599
Size: 397 8 789 405
Fragments arranged by size:
789 405 397 8
SacI G_AGCT'C
Cuts at: 0 1268 1599
Size: 1268 331
SapI GCTCTTCn'nnn_
Cuts at: 0 1567 1599
Size: 1567 32
SexAI A'CCwGG_T
Cuts at: 0 527 1139 1599
Size: 527 612 460
Fragments arranged by size:
612 527 460
SfcI C'TryA_G
Cuts at: 0 237 323 445 1579 1599
Size: 237 86 122 1134 20
Fragments arranged by size:
1134 237 122 86 20
SfoI GGC'GCC
Cuts at: 0 122 1215 1599
Size: 122 1093 384
Fragments arranged by size:
1093 384 122
SmaI CCC'GGG
Cuts at: 0 82 1599
Size: 82 1517
SmlI C'TyrA_G
Cuts at: 0 1034 1599
Size: 1034 565
StuI AGG'CCT
Cuts at: 0 313 370 1223 1599
Size: 313 57 853 376
Fragments arranged by size:
853 376 313 57
StyI C'CwwG_G
Cuts at: 0 308 549 1468 1599
Size: 308 241 919 131
Fragments arranged by size:
919 308 241 131
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1050 1599
Size: 1050 549
TatI w'GTAC_w
Cuts at: 0 815 1599
Size: 815 784
Tth111I GACn'n_nGTC
Cuts at: 0 709 1075 1599
Size: 709 366 524
Fragments arranged by size:
709 524 366
XmaI C'CCGG_G
Cuts at: 0 80 1599
Size: 80 1519
ZraI GAC'GTC
Cuts at: 0 1434 1599
Size: 1434 165
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AlfI AlwNI ApaI
ApoI AvaI AvrII BanI BanII BbeI BbsI BbvCI
BciVI BfrBI BglII BlpI Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BseRI
BseYI BsiHKAI Bsp1286I BsrBI BsrFI BssSI BstEII BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII HpaI KasI KpnI MmeI MscI MslI MspA1I
NarI NsiI PasI PpiI PspOMI PstI PvuII SacI
SapI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI Tth111I XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AleI AloI
ApaLI AscI AseI AsiSI BaeI BamHI BcgI BclI
BglI BmtI BsaBI BsaXI BsgI BsiEI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrDI BsrGI BssHII BstAPI
BstBI BstXI ClaI CspCI DraI DraIII EagI EcoRV
FalI FseI FspI FspAI HindIII MfeI MluI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NspI PacI
PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI TstI XbaI XcmI XhoI XmnI